Comparison of TerpeneProfiles of Chamaecyparis thyoides...
Transcript of Comparison of TerpeneProfiles of Chamaecyparis thyoides...
![Page 1: Comparison of TerpeneProfiles of Chamaecyparis thyoides ...atlantic-white-cedar.org/pubs/AWC2012proceedings/pdf-awc/Waters… · Presented at the Ecology & Management of Atlantic](https://reader036.fdocuments.in/reader036/viewer/2022071217/604a37efc6c72e4aa80bf6fc/html5/thumbnails/1.jpg)
Kristen A. Hallock‐Waters, Jessica Favorito, Kimberlee Downey, Amanda Burkert, & George Zimmermann, The Richard Stockton College of New Jersey
Presented at the Ecology & Management of Atlantic White Cedar Symposium Suffolk, VA June 12‐14, 2012
Comparison of Terpene Profiles of Chamaecyparis thyoidesFoliage at Various Levels of Deer Browse from Long‐term
Study Sites
![Page 2: Comparison of TerpeneProfiles of Chamaecyparis thyoides ...atlantic-white-cedar.org/pubs/AWC2012proceedings/pdf-awc/Waters… · Presented at the Ecology & Management of Atlantic](https://reader036.fdocuments.in/reader036/viewer/2022071217/604a37efc6c72e4aa80bf6fc/html5/thumbnails/2.jpg)
What is a terpene?
Terpenes are carbon based secondary metabolites synthesized by plants, particularly conifers.
They have many functions including acting as a protective chemical defense against herbivory.
![Page 3: Comparison of TerpeneProfiles of Chamaecyparis thyoides ...atlantic-white-cedar.org/pubs/AWC2012proceedings/pdf-awc/Waters… · Presented at the Ecology & Management of Atlantic](https://reader036.fdocuments.in/reader036/viewer/2022071217/604a37efc6c72e4aa80bf6fc/html5/thumbnails/3.jpg)
Monoterpenes: C10H16Sesquiterpenes: C15H24Diterpenes: C20H32
Examples:
Oxidation produces compounds referred to as terpenoids.
monoterpenes
sesquiterpenes
diterpenebeyerene
![Page 4: Comparison of TerpeneProfiles of Chamaecyparis thyoides ...atlantic-white-cedar.org/pubs/AWC2012proceedings/pdf-awc/Waters… · Presented at the Ecology & Management of Atlantic](https://reader036.fdocuments.in/reader036/viewer/2022071217/604a37efc6c72e4aa80bf6fc/html5/thumbnails/4.jpg)
Previous Research on Other Species
Based on olfactory and taste senses, herbivory preferentially browse vegetation with lower concentrations of terpenes(Cooper & Owen, 1985; Suomela, et al., 1997).
Oxygenated monoterpenes may inhibit microbial action in ruminants impairing digestion (Oh et al., 1967; Schwartz et al., 1980; Vourc’h et al., 2002).
![Page 5: Comparison of TerpeneProfiles of Chamaecyparis thyoides ...atlantic-white-cedar.org/pubs/AWC2012proceedings/pdf-awc/Waters… · Presented at the Ecology & Management of Atlantic](https://reader036.fdocuments.in/reader036/viewer/2022071217/604a37efc6c72e4aa80bf6fc/html5/thumbnails/5.jpg)
The production of terpenesin conifers as a chemical defense can be stimulated by herbivory (Bohlmann & Croteau, 1999; Lewinsohn et al., 1991).
Defoliation by herbivory may reduce the carbon pool available to produce secondary metabolites (Bryant et al., 1983; Raffa & Smalley, 1995).
![Page 6: Comparison of TerpeneProfiles of Chamaecyparis thyoides ...atlantic-white-cedar.org/pubs/AWC2012proceedings/pdf-awc/Waters… · Presented at the Ecology & Management of Atlantic](https://reader036.fdocuments.in/reader036/viewer/2022071217/604a37efc6c72e4aa80bf6fc/html5/thumbnails/6.jpg)
Colletti SiteBrendan T. Byrne State Forest
1) Herbicide/ Electric Fence
2) Herbicide/ No Deer Control
3) Herbicide/ Hinder
4) Control/No treatment
![Page 7: Comparison of TerpeneProfiles of Chamaecyparis thyoides ...atlantic-white-cedar.org/pubs/AWC2012proceedings/pdf-awc/Waters… · Presented at the Ecology & Management of Atlantic](https://reader036.fdocuments.in/reader036/viewer/2022071217/604a37efc6c72e4aa80bf6fc/html5/thumbnails/7.jpg)
Terpene Extraction• 1.00 g AWC foliage extracted in 10mL Ultima‐grade methanol at room temperature for 48 hrs.
• Foliage from each tree extracted in triplicate.
• Extract decanted & syringe filtered through 0.45 m PTFE filter.
• Deuterated toluene added to a concentration of 10.0 ppm as an internal standard.
• Extracts stored at ‐70C in air tight aluminum crimp seal vials until analysis by gas chromatography/mass spectrometry (GC/MS).
![Page 8: Comparison of TerpeneProfiles of Chamaecyparis thyoides ...atlantic-white-cedar.org/pubs/AWC2012proceedings/pdf-awc/Waters… · Presented at the Ecology & Management of Atlantic](https://reader036.fdocuments.in/reader036/viewer/2022071217/604a37efc6c72e4aa80bf6fc/html5/thumbnails/8.jpg)
GC/MS Analysis
Agilent 6890N GC/5973 Mass Selective Detector
All extracts analyzed in triplicate using GC/MS.
Carrier Gas: He
Flow Rate: 1.0 mL/min
Injection Volume: 2.0 L
Injector Temperature: 270 C
Column Temperature : 60 – 300 C; ramp 3 C/min
Column: Agilent HP-5MS; 0.25mm x 30 m x 0.25m
Split Ratio: 1:20
![Page 9: Comparison of TerpeneProfiles of Chamaecyparis thyoides ...atlantic-white-cedar.org/pubs/AWC2012proceedings/pdf-awc/Waters… · Presented at the Ecology & Management of Atlantic](https://reader036.fdocuments.in/reader036/viewer/2022071217/604a37efc6c72e4aa80bf6fc/html5/thumbnails/9.jpg)
Ionization source: EI (electron impact)
Source Temperature: 200 C
The sample molecules eject an electron leaving behind a molecular ion with a positive charge.
e-
M M+• + e-
molecule radicalcation
The molecular ion is inherently unstable and fragments. Bonds break resulting in fragments having a lower mass than the molecular ion.
The mass fragmentation pattern of a molecule is a characteristic similar to a fingerprint – it can aid in identification.
![Page 10: Comparison of TerpeneProfiles of Chamaecyparis thyoides ...atlantic-white-cedar.org/pubs/AWC2012proceedings/pdf-awc/Waters… · Presented at the Ecology & Management of Atlantic](https://reader036.fdocuments.in/reader036/viewer/2022071217/604a37efc6c72e4aa80bf6fc/html5/thumbnails/10.jpg)
Total Ion ChromatogramAWC Extract – Minimal Browsing
monoterpenes sesquiterpenes diterpenes
![Page 11: Comparison of TerpeneProfiles of Chamaecyparis thyoides ...atlantic-white-cedar.org/pubs/AWC2012proceedings/pdf-awc/Waters… · Presented at the Ecology & Management of Atlantic](https://reader036.fdocuments.in/reader036/viewer/2022071217/604a37efc6c72e4aa80bf6fc/html5/thumbnails/11.jpg)
Total Ion ChromatogramAWC Extract - Heavily Browsed
monoterpenes sesquiterpenes diterpenes
![Page 12: Comparison of TerpeneProfiles of Chamaecyparis thyoides ...atlantic-white-cedar.org/pubs/AWC2012proceedings/pdf-awc/Waters… · Presented at the Ecology & Management of Atlantic](https://reader036.fdocuments.in/reader036/viewer/2022071217/604a37efc6c72e4aa80bf6fc/html5/thumbnails/12.jpg)
Total Ion ChromatogramAWC Extract – Minimal Browsing
Total Ion ChromatogramAWC Extract - Heavily Browsed
monoterpenes sesquiterpenes diterpenes
monoterpenes sesquiterpenes diterpenes
![Page 13: Comparison of TerpeneProfiles of Chamaecyparis thyoides ...atlantic-white-cedar.org/pubs/AWC2012proceedings/pdf-awc/Waters… · Presented at the Ecology & Management of Atlantic](https://reader036.fdocuments.in/reader036/viewer/2022071217/604a37efc6c72e4aa80bf6fc/html5/thumbnails/13.jpg)
Molecular ion m/z = 136monoterpene
![Page 14: Comparison of TerpeneProfiles of Chamaecyparis thyoides ...atlantic-white-cedar.org/pubs/AWC2012proceedings/pdf-awc/Waters… · Presented at the Ecology & Management of Atlantic](https://reader036.fdocuments.in/reader036/viewer/2022071217/604a37efc6c72e4aa80bf6fc/html5/thumbnails/14.jpg)
![Page 15: Comparison of TerpeneProfiles of Chamaecyparis thyoides ...atlantic-white-cedar.org/pubs/AWC2012proceedings/pdf-awc/Waters… · Presented at the Ecology & Management of Atlantic](https://reader036.fdocuments.in/reader036/viewer/2022071217/604a37efc6c72e4aa80bf6fc/html5/thumbnails/15.jpg)
Molecular ion m/z = 286C20H30O
![Page 16: Comparison of TerpeneProfiles of Chamaecyparis thyoides ...atlantic-white-cedar.org/pubs/AWC2012proceedings/pdf-awc/Waters… · Presented at the Ecology & Management of Atlantic](https://reader036.fdocuments.in/reader036/viewer/2022071217/604a37efc6c72e4aa80bf6fc/html5/thumbnails/16.jpg)
![Page 17: Comparison of TerpeneProfiles of Chamaecyparis thyoides ...atlantic-white-cedar.org/pubs/AWC2012proceedings/pdf-awc/Waters… · Presented at the Ecology & Management of Atlantic](https://reader036.fdocuments.in/reader036/viewer/2022071217/604a37efc6c72e4aa80bf6fc/html5/thumbnails/17.jpg)
C10H164.7%
Other19.9%
C15H24Ox40.4%
C15H245.9%
C20H3217.6%
C20H32Ox5.4%
C10H16Ox0.8% C12H20O2
1.0%
% Total Ion ChromatogramMinimally Browsed AWC (lower 4ft)
![Page 18: Comparison of TerpeneProfiles of Chamaecyparis thyoides ...atlantic-white-cedar.org/pubs/AWC2012proceedings/pdf-awc/Waters… · Presented at the Ecology & Management of Atlantic](https://reader036.fdocuments.in/reader036/viewer/2022071217/604a37efc6c72e4aa80bf6fc/html5/thumbnails/18.jpg)
Other24.7%
C15H24Ox54.7%
C15H249.9%
C10H164.3%
C20H329.9%
% Total Ion ChromatogramMinimally Browsed AWC (above 4ft )
C10H16Ox1.5%
C12H20O21.4%
C20H32Ox1.9%
![Page 19: Comparison of TerpeneProfiles of Chamaecyparis thyoides ...atlantic-white-cedar.org/pubs/AWC2012proceedings/pdf-awc/Waters… · Presented at the Ecology & Management of Atlantic](https://reader036.fdocuments.in/reader036/viewer/2022071217/604a37efc6c72e4aa80bf6fc/html5/thumbnails/19.jpg)
C10H164.4%
C15H248.8%
C15H24Ox53.6%
C20H326.3%
Other20.9%
C20H32Ox1.6%
C10H16Ox0.3% C12H20O2
1.6%
%Total Ion ChromatogramSemi-browsed AWC (lower 4 ft)
![Page 20: Comparison of TerpeneProfiles of Chamaecyparis thyoides ...atlantic-white-cedar.org/pubs/AWC2012proceedings/pdf-awc/Waters… · Presented at the Ecology & Management of Atlantic](https://reader036.fdocuments.in/reader036/viewer/2022071217/604a37efc6c72e4aa80bf6fc/html5/thumbnails/20.jpg)
Other17.0%
C10H168.8%
C15H246.7%
C15H24Ox48.6%
C10H168.8%
C15H246.7%
C20H325.2%
C20H32Ox3.8%
%Total Ion ChromatogramHeavily Browsed AWC C10H16Ox
0.5%C12H20O22.0%
![Page 21: Comparison of TerpeneProfiles of Chamaecyparis thyoides ...atlantic-white-cedar.org/pubs/AWC2012proceedings/pdf-awc/Waters… · Presented at the Ecology & Management of Atlantic](https://reader036.fdocuments.in/reader036/viewer/2022071217/604a37efc6c72e4aa80bf6fc/html5/thumbnails/21.jpg)
ConclusionsMonoterpene contribution to the total ion chromatogram in heavily browsed AWC foliage was nearly twice that observed in minimally browsed and semi-browsed trees. This may be result of a stress response to herbivory.
OR
Measuring remaining AWC that survived and perhaps these trees survived because a higher abundance of monoterpenes made them less palatable.
![Page 22: Comparison of TerpeneProfiles of Chamaecyparis thyoides ...atlantic-white-cedar.org/pubs/AWC2012proceedings/pdf-awc/Waters… · Presented at the Ecology & Management of Atlantic](https://reader036.fdocuments.in/reader036/viewer/2022071217/604a37efc6c72e4aa80bf6fc/html5/thumbnails/22.jpg)
ConclusionsOxygenated monoterpenoids which inhibit microbial action in ruminants making digestion difficult represented only 2-3% of the total ion chromatogram regardless of browse level.
The diterpene fraction represented 23% of the total ion chromatogram for minimally browsed AWC foliage, but only 8-9% for browsed foliage suggesting browsed AWC lack the carbon pool to synthesize the larger terpenes.
![Page 23: Comparison of TerpeneProfiles of Chamaecyparis thyoides ...atlantic-white-cedar.org/pubs/AWC2012proceedings/pdf-awc/Waters… · Presented at the Ecology & Management of Atlantic](https://reader036.fdocuments.in/reader036/viewer/2022071217/604a37efc6c72e4aa80bf6fc/html5/thumbnails/23.jpg)
Future Directions
Increase sample population!!!
Investigate terpene profile:
geographically seasonally intra-tree greenhouse experiments & browsing studies
![Page 24: Comparison of TerpeneProfiles of Chamaecyparis thyoides ...atlantic-white-cedar.org/pubs/AWC2012proceedings/pdf-awc/Waters… · Presented at the Ecology & Management of Atlantic](https://reader036.fdocuments.in/reader036/viewer/2022071217/604a37efc6c72e4aa80bf6fc/html5/thumbnails/24.jpg)
Acknowledgements
Past undergraduate students:Victoria JohnsonDana BezaireAlyssa SchlueterRyan Roff
Support: The Richard Stockton College of New Jersey, Career Development Award