Mucosal healing and risk for lymphoproliferative malignancy in celiac disease
Lymphoproliferative Disease Virus (LPDV)whippslab.weebly.com/uploads/3/1/6/2/31625533/... ·...
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Epidemiology and Genetic Analysis of
Lymphoproliferative Disease Virus (LPDV) of Wild Turkeys (Meleagrisy ( ggallopavo) in New York
Katrina Alger1
Elizabeth Bunting VMD2, Krysten Schuler PhD2Krysten Schuler PhD2,
Christopher M. Whipps PhD1
Affiliations: 1SUNY-ESF, State University of New York College of Environmental Science and Forestry, Department of Environmental and
Forest Biology, Syracuse, NY, 2Animal Health Diagnostic Center, Department of Population Medicine and Diagnostic Services, College of
Veterinary Services, Cornell University, Ithaca, NY
Lymphoproliferative Disease Virus (LPDV)
• Rarest of three oncogenic avian retroviruses
• In domestic flocks rapid clinical course with few signs In domestic flocks, rapid clinical course with few signs prior to death and high flock mortality
• Signs of disease in wild birds:• External tumors on head, neck, and feet,
similar to pox
• Internal lesions on spleen liver thymus
Photo credit: Allison, et. al (2014) - Virology, 450-451, 2–12
• Internal lesions on spleen, liver, thymus, and other organs
• Many birds appear asymptomatic (lack gross lesions)
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LPDV in Wild Turkeys• First detection in wild turkeys by Southeastern
Cooperative Wildlife Disease Study (SCWDS) in 2009
W d d • Widespread -estimated prevalence for states ranges from 25-85%
Vi • Virus appears endemic in United States
Confirmed LPDV Cases
Thomas, et. al (2015) – PLOSOne DOI:10.1371/journal.pone.0122644; Allison, et. al (2014) - Virology, 450-451, 2–12Map courtesy of Justin Brown, DVM (PA Game Commission)
Objectives1. Develop a simple, reliable method for diagnosing LPDV in
living birds using PCR of whole blood and blood clots; track disease progress in living birds via telemetry data/band returns
2. Determine prevalence of LPDV in wild turkeys in New York State; investigate potential demographic, environmental, and anthropogenic risk factors for infection
3 A l ti l di t ib ti f LPDV i f ti i N Y k 3. Analyze spatial distribution of LPDV infection in New York by looking for clusters of higher or lower than expected prevalence; create a risk surface map displaying pattern of estimated prevalence throughout state
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Whole Blood vs. Buffy Coat
Whole Blood vs. Bone Marrow
Result 95% CI Result 95% CI Apparent prevalence 81% 73-87% 75% 57-89%True prevalence 83% 76-89% 72% 53-86%
Sensitivity 97% 93-99% 100% 79-100%y
Specificity 100% 79-100% 89% 52-100%Cohen's kappa 0.93 0.84-1.00 0.92 0.77-1.07P-value <0.0001 <0.0001
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N=2536; Prevalence=55% (95% CI: 53-57)
No difference across study years:2012-2013: X2=1.77, p=0.1842012-2014: X2=2.98, p=0.0842013-2014: X2=0.068, p=0.793, p
Risk Factors
•Odds of testing positive f LPDV for LPDV were:• Adult > Juvenile
• Female > Male
• Counties with steady fall h i < harvest estimates < Counties with decline in 2012-2014 fall harvest
http://www.gameandfishmag.com/forecasts/oklahoma-turkey-forecast-2015/
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Possible Explanations• Juveniles falling below detection threshold
LPDV l t d lt t lit b i k d?
So what?
• LPDV – related poult mortality being masked?
• Social dynamics of turkey flocks (age and sex segregation)
• Females drive population via production
• Importance of subclinical infections
• Transmission implications – multiple routes?
Dual Kernel Density Estimation• Cases/Controls• Adaptive bandwidth
smoothing parameter
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Clusters of low prevalence
Anselin’sLocal Moran’s I (LISA), p<0.001
SaTScan(Kulldorff’sspatial scan t ti ti )statistic),
p=0.007
Cluster of high prevalenceAnselin’s Local Moran’s I (LISA), p≤ 0.001
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Sequencing• 138 unique sequences • 56 samples from GenBank
(Allison et al. 2014 & Thomas et al. 2015)
• 18 additional New York samples• 5 from Clade 1 (Israel and South Carolina)• 33 samples from other New England states that
received translocated turkeys from New York
Clades often shared common translocation origin
Birds l d translocated
from Sullivan County
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Unknown relationship
Common origin of translocated birdsbirds
Common origin of translocated birds and geographic association
Geographic association
82% (23/28) clades could be explained geographically, or by translocation records
Future directions1. Track progression of
disease in living birds
l2. Investigate potential reproductive or immunological impacts of LPDV (i.e. decreased production, or increased susceptibility to cosusceptibility to co-infections)
3. Continue monitoring relationship between LPDV and harvest decline
Photo by: Rick Taylor
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Funding:• Department of Environmental
and Forest Biology• New York State Department of Environmental Conservation• Edna Bailey Sussman Foundation• Animal Health Diagnostic Center at Cornell University
Special Thanks:• My committee: Dr. Chris Whipps, Dr. Krysten Schuler,
Dr. Beth Bunting, Dr. Shannon Farrell, and Dr. Richard Hawks
k h d f ld b l ( )
Animal Health Diagnostic Center at Cornell University• ESF Graduate School
Photo credit: National Wild Turkey Federation
• Mike Schiavone and field biologists (NYSDEC)• Nikki Dean and Alyssa Wetterau (Cornell)• Josh Enck, Kristen Doerr, Elle Palmer, Tiquasha Thompson,
Cassandra Elliot (SUNY-ESF)• Jesse Thomas (UGA) & Justin Brown DVM (PA Game
Commission, formerly SCWDS)
Questions?
http://funny-pics-fun.com/funny-compilations/thanksgiving-day-turkey