Ebola Virus Disease

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Ebola Virus Disease. วิเชฎฐ์ ยาทองไชย นายสัตวแพทย์ชำนาญการพิเศษ สำนักงานปศุสัตว์จังหวัดสกลนคร. ตระกูลและสายพันธุ์. Family: Filoviridae Filovirus Genus: Marburgvirus Ebolavirus Cuevavirus. Nakayama, E., & Saijo , M., 2013. Marburgvirus. 1967 Marburg ในเยอรมนี และ ยูโกสลาเวีย - PowerPoint PPT Presentation

Transcript of Ebola Virus Disease

Ebola Virus Disease

Ebola Virus Disease 2Family: FiloviridaeFilovirusGenus: MarburgvirusEbolavirusCuevavirus

Nakayama, E., & Saijo, M., 2013

Marburgvirus 1967_Germany and yugoslavia2Marburgvirus1967Marburg (Africal green vervet monkey) 31 7 3

during an outbreak in laboratory staff exposed to tissues from monkeys imported from Uganda.34Family: FiloviridaeFilovirusGenus: MarburgvirusEbolavirusCuevavirus

Nakayama, E., & Saijo, M., 2013

Marburgvirus 1967_Germany and yugoslavia4Cuevavirus2010 Lloviu cuevavirus Miniopterus schreibersii filovirus 5

56Family: FiloviridaeFilovirusGenus: MarburgvirusEbolavirusCuevavirus

Nakayama, E., & Saijo, M., 2013

Marburgvirus 1967_Germany and yugoslavia6Ebola VirusGenus Ebolavirus is 1 of 3 members of the Filoviridae family (filovirus). Genus Marburg virus and genus Cueva virus. Comprises 5 distinct species: Bundibugyo ebolavirus (BDBV) Zaire ebolavirus (EBOV) Reston ebolavirus (RESTV) Sudan ebolavirus (SUDV) Ta Forest ebolavirus (TAFV)

Ebola Virus DiseaseBDBV EBOV SUDV EVD RESTV Ebola Virus Disease : EVD/ 50-90%

Group 4 biological agent means one that causes severe human disease and is a serious hazard to workers; it may present a high risk of spreading to the community;there is usually no effective prophylaxis or treatment available.


10Ebola Virus Disease

Ebola Virus Disease2-21 Hypsignathus monstrosus(hammer-headed fruit bat), Epomops franqueti(Franquet's epauletted bat) Myonycteris torquata(little collared fruit bat) (host)

Hypsignathus monstrosus(hammer-headed fruit bat)

Epomops franqueti(Franquet's epauletted bat)

Myonycteris torquata(little collared fruit bat)

1976 151 284 (53%) - (Sudan; SEBOV) (: ) 280 318 (88%) - (Zaire; ZEBOV)

18Feldmann & Geisbert, 2010


18- . Chloroquin 5 . 7

82 (25% ) 191976- Nzara128 . Maridi

201976 Nzara3 48 Maridi 128 .


1994TAFV made its first and thus far only known appearance in 1994 during a viral hemorrhagic feverepizooticamong western chimpanzees (Pan troglodytes verus) inTa National Park,Cte d'Ivoire. As more dead western chimpanzees were discovered, many tested positive for infection with an ebolavirus distinct from those already known. One of the scientists performing the necropsies on the infected western chimpanzees contracted TAFV. She developed symptoms similar to those ofdengue feverapproximately a week after the necropsy, and was transported to Switzerland for treatment. She was discharged from hospital after two weeks and had fully recovered six weeks after the infection.

1994 (W) 2wk . 6wk



- 34%22




2014While investigating on an outbreak ofSimian hemorrhagic fever(SHFV) in November 1989, anelectron microscopistfromUSAMRIIDnamed Thomas W. Geisbert discovered filoviruses similar in appearance toEbola virusin tissue samples taken fromCrab-eating Macaqueimported from thePhilippinestoHazleton Laboratoriesin Reston, Virginia. The filovirus was further isolated by Dr.Peter B. Jahrling, and over the period of three months over a third of the monkeys diedat a rate of two or three a day.[10]Blood samples were taken from 178 animal handlers during the incident.[11]Of them, six eventuallyseroconverted, testing positive usingELISA. They remained, however, asymptomatic. In January 1990, an animal handler at Hazelton cut himself while performing anecropsyon the liver of an infected Cynomolgus. Under the direction of theCenter for Disease Control and Prevention(CDC) the animal handler was placed under surveillance for the duration of theincubation period. When the animal handler failed to become ill, it was concluded that the virus had a lowpathogenicityin humans.[12]The monkeys were imported from the Philippines, which had no previous record of SHFV or ebolavirus infections23RESTV EVD (macaque monkeys:Macaca fascicularis) ()

Ebola-R) .. 1989, 1990 1996 1992 YearCountryEbolavirus speciesCase fatality2012Democratic Republic of CongoBundibugyo51%2012UgandaSudan57%2012UgandaSudan71%2011UgandaSudan100%2008Democratic Republic of CongoZaire44%2007UgandaBundibugyo25%2007Democratic Republic of CongoZaire71%2005CongoZaire83%2004SudanSudan41%2003 (Nov-Dec)CongoZaire83%2003 (Jan-Apr)CongoZaire90%2001-2002CongoZaire75%2001-2002GabonZaire82%2000UgandaSudan53%1996South Africa (ex-Gabon)Zaire100%1996 (Jul-Dec)GabonZaire75%1996 (Jan-Apr)GabonZaire68%1995Democratic Republic of CongoZaire81%1994Cote d'IvoireTa Forest0%1994GabonZaire60%1979SudanSudan65%1977Democratic Republic of CongoZaire100%1976SudanSudan53%1976Democratic Republic of CongoZaire88%25WHO, 20142. ???

?? 2-3 Antigen-capture enzyme-linked immunosorbent assay (ELISA) testingIgM ELISART-PCR/ real-time RT-PCRVirus isolationIgM and IgG antibodiesImmunohistochemistry testingRT-PCR/ real-time RT-PCRVirus isolation ???

Texas Biomedical Research Institute antigen-RNA genes ( , 2557) ??? (dried material) 4C

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( reservoir host)


Center of Disease Control and Prevention (CDC), 2014World Health Organization website (WHO): Ebola factsheetTakada, A. (2012). Filovirus tropism: cellular molecules for viral entry. Frontiers in microbiology, 3.Nakayama, E., & Saijo, M. (2013). Animal models for Ebola and Marburg virus infections.Frontiers in microbiology,4.Rouquet, P., Froment, J. M., Bermejo, M., Kilbourn, A., Karesh, W., Reed, P., ... & Leroy, E. M. (2005). Wild animal mortality monitoring and human Ebola outbreaks, Gabon and Republic of Congo, 2001-2003. Emerging infectious diseases, 11(2).http://followtheoutbreak.wordpress.com/2014/04/30/re- emergence-of-ebola-focuses-need-for-global-surveillance- strategies/https://microbewiki.kenyon.edu/index.php/Infection_Mechanism_of_Genus_Ebolavirus