HPHE 6720 - Topic 4

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    Topic 04:

    Anthropometry and BodyComposition Measurement

    Laboratory Manual Section 04

    HPHE 6720

    Dr. Cheatham

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    Outline

    Importance of Body Composition

    General Principles Basic principles

    Anthropometric Methods Body Mass Index (BMI)

    Circumferences / Waist to Hip Ratio

    Skinfold Measurements

    Densitometry Methods Hydrostatic (Underwater) Weighing

    Plethysmography (Air Displacement)

    Other Methods Bioelectrical Impedance Analysis (BIA)

    Dual Energy X-Ray Absorptiometry (DEXA)

    Interpretation of Results

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    Introduction

    Definition: Relative proportion of fat and fat-free tissue in the body.

    Clinical significance: Obesity is correlated to an

    increased risk of: Coronary artery disease (CAD)

    Noninsulin-dependent diabetes mellitus (NIDDM)

    Hypertension (HTN) Certain cancers

    Hyperlipidemia (high blood cholesterol)

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    Why Do We Measure BC?

    Excess body fat is associated with: Hypertension, Type 2 Diabetes, Stroke, CAD, Hyperlipidemia

    To assess the decrease in body fat weight that occurs

    in response to a weight management program.

    To help athletes determine the best body composition

    for performance.

    To monitor fat and fat-free weight in patients with

    disease.

    To track long-term changes that occur in body fat and

    fat-free mass with aging.

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    Basic Principles

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    Basic Principles

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    Terminology

    Percent Body Fat (%BF)

    The percentage of the bodyweight that is adipose tissue

    Fat Weight (FW)

    The total weight of the adipose tissue

    Fat-Free Weight (FFW)

    The total weight of everything that is not fat Lean Body Mass (LBW)

    More so refers to the weight of muscle

    Overweight

    Deviation in body weight from some standard or ideal weight in relation to

    height

    Overfat

    Undesirable percent body fat (difference males vs. females)

    Obesity

    Surplus of adipose tissue resulting from excessive energy intake relative to

    energy expenditure

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    Body Composition Techniques

    Definition: Measurement of the human body

    Techniques include:

    Height and weight (body mass index [BMI], waist-to-hip

    ratio [WHR])

    Circumferences and girths

    Skinfolds

    Bioelectrical impedance analysis

    Hydrostatic weighing

    Purpose: To evaluate body weight and composition

    in the health and fitness field, often to establish an

    individuals target, desirable, or optimal weight

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    Anthropometry - Height and Weight

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    Anthropometry - Body Mass Index (BMI)

    Body mass index = __Weight in kg__(Height in meters)2

    Statistics: For most people, obesity-related health problems

    increase beyond a BMI of 25.0

    In increased risk of hypertension, TC/HDL ratio, CAD andmortality rate are associated with a BMI 30

    Limitations: It is difficult for clients to interpret weight loss and gain

    There is no differentiation between fat weight and fat-free weight

    There is only a modest correlation with percent body fatdetermined by hydrostatic weight

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    Anthropometry - Body Mass Index (BMI)

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    Anthropometry - Body Mass Index (BMI)

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    Anthropometry - Circumferences

    Uses: Pre-post changes in body measurements

    Useful for weight management programs/motivation forclients/patients

    Waist circumference used along with BMI can be used

    as an estimate of disease risk (ACSM Manual, Table 4-3) Estimate % BF (limited accuracy)

    Calculate Waist-to-Hip Ratio

    Advantages: Easily learned Quickly administered

    Quantifies changes in muscle with specific training(muscle girth size)

    Easy to document changes in body size

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    Anthropometry - Waist Circumference

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    Anthropometry - Circumferences - Sites

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    Anthropometry - Circumferences - Sites

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    Anthropometry - Circumferences - Procedures

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    Anthropometry - Waist-to-Hip Ratio

    Definition Comparison between the circumference of the

    waist and hips, representing the personsdistribution of body fat

    WHR = Waist circumference (cm)

    Hip circumference (cm)

    Waist circumference alone may be used as an

    indicator of health risk Truncal adiposity increases the risk of chronic

    disease

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    Anthropometry - Waist-to-Hip Ratio

    The pattern of body fatdistribution is an important

    predictor of the health risks

    of obesity.

    Android (male pattern)

    Trunk/abdominal fat

    Increased risk for hypertension,

    diabetes, dyslipidemia, CAD,

    premature death Gynoid (female pattern

    Hip and thigh

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    Anthropometry - Waist-to-Hip Ratio

    ACSM Box 4-1 (Waist)

    ACSM Box 4-1 (Buttocks/Hips)

    Health risks are very high forthe young when:

    Males > 0.95

    Females > 0.86

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    Anthropometry - Waist-to-Hip Ratio

    Waist Measurement

    Hip Measurement

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    Anthropometry - Skinfold Assessment

    Principle ~33% of our total body

    fat lies directlybeneath the skin

    (subcutaneous)

    Therefore, bymeasuring skinfold

    thickness we canestimate body densityand % body fat

    Accuracy ( 4-5%)

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    Anthropometry - Skinfold Assessment - Sites

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    Anthropometry - Skinfold Assessment - Sites

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    Anthropometry - SkinfoldSites (Chest)

    Diagonal fold; one-half the distance between the anterior axillary line and the

    nipple (men), or one-third of the distance between the anterior axillary line and

    the nipple (women)

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    Anthropometry - SkinfoldSites (Abdomen)

    Vertical fold; 2 cm to the right side of the umbilicus

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    Anthropometry - SkinfoldSites (Thigh)

    Vertical fold; on the anterior midline of the thigh, mid-way between the proximal

    border of the patella and the inguinal crease (hip)

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    Anthropometry - SkinfoldSites (Tricep)

    Vertical fold; on the posterior midline of the upper arm, halfway between the

    acromion and olecranon processes, with the arm held freely to the side of the

    body

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    Anthropometry - SkinfoldSites (Suprailiac)

    Diagonal fold; in line with the natural

    angle of the iliac crest taken in the anterioraxillary line immediately superior to the

    iliac crest

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    Anthropometry - SkinfoldSites (Midaxillary)

    Vertical fold; on the midaxillary line at

    the level of the xiphoid process of the

    sternum.

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    Anthropometry - SkinfoldSites (Subscapular)

    Diagonal fold (at a 45 degree angle); 1to 2 cm below the inferior angle of the

    scapula

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    Anthropometry - SkinfoldSites (Calf)

    Vertical fold; at the maximumcircumference of the calf on the midline

    of its medial border

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    Anthropometry - Skinfolds Assessment - Procedures

    Technique: Firmly grasp all subcutaneous fat (without muscle)

    Use two fingers (thumb and index) about 8 cmapart, perpendicular to long axis of site 1 cm above

    the site to be measured

    Release the scissor grip of the caliper but supportits weight while measuring no longer than 1 to 2seconds to the nearest 0.5 mm

    Measure each site at least two times, rotatingthrough the sites (should be within 1 to 2 mm)

    Use the average of each skinfold site for use in theregression formula

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    Anthropometry - Skinfolds Assessment - Procedures

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    Anthropometry - Skinfolds Assessment - Procedures

    Note: Make sure to carry body density out to three decimal places.

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    Anthropometry - Skinfolds Assessment - Procedures

    Note: Make sure to carry body density out to three decimal places.

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    Anthropometry - Skinfolds Assessment - Procedures

    Converting Body Density to % Body Fat Generalized Equations:

    % Fat = (457 / Body Density) 414.2 (Brozek)

    % Fat = (495 / Body Density) 450 (Siri)

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    Anthropometry - Skinfolds Assessment - Procedures

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    Densitometry - Hydrostatic Weighing

    Measurement of Density Density = mass/volume

    Mass = The bodyweight we measure in air onthe scale

    Volume = Why we are doing hydrostatic

    weighing Use of hydrostatic weighing to measure

    volume Based on Archimedes Principle:

    "When a solid body is partially or completelyimmersed in water, the apparent loss in weightwill be equal to the weight of the displacedliquid.

    By knowing the density of water, it is easy to gofrom weight to volume.

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    Densitometry - Hydrostatic Weighing

    d h

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    Densitometry - Hydrostatic Weighing

    Considered the Gold Standard Measurement of Body Density

    Underwater, muscle and bone is more dense than

    fat Therefore, the more muscle/bone, the more the

    subject will weigh underwater or vice versa

    By weighing the subject in the air AND underwater,

    we can measure BODY DENSITY

    From body density, % body fat is calculated

    Accuracy ( 3%)

    i d i i hi

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    Densitometry - Hydrostatic Weighing

    More Muscle More FatSame Weight

    D i H d i W i hi

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    Densitometry - Hydrostatic Weighing

    Body Density =

    BWAIR

    BWAIR (BWUWW Tare)

    H2O Density- (RV +100)( )( )

    BWAIR = Bodyweight in air in gramsBWUW = Bodyweight underwater in grams

    Tare = Chair weight (and any other apparatus) in grams

    H2O Density = Density of water at specific temperature

    RV = Residual volume in mL

    Residual Volume (males) = (0.027 * Ht) + (0.017 * age) - 3.45

    Residual Volume (females) = (0.032 * Ht) + (0.009 * age) - 3.90

    Residual Volume (both) = 0.24 x FVC

    % Body Fat = (457 body density) 414.2 (Brozek)

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    Densitometry - Air Displacement Plethysmography

    Bi l t i l I d A l i

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    Bioelectrical Impedance Analysis

    Premise: The volume of fat-free tissue is proportional to the

    electrical conductivity of the body

    Fat (tissue with little water: 14% to 22%) is a poorelectrical conductor

    Lean tissue (mostly water: >90%) is a good electrical

    conductor

    Accuracy is highly variable

    Greatly affected by caffeine, alcohol, fluid intake

    Bi l t i l I d A l i22

    XcRZ 22

    XcRZ

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    Bioelectrical Impedance Analysis

    Principle:

    Impedance (Z): A measure of how an electrical current is

    slowed or stopped as it passes through a material.

    Impedance has two components: Resistance (R) and

    Reactance (Xc). Approximately 95% of impedance is

    determined by resistance.

    Resistance (R): A measure of the amount of electrical

    current a substance will stop.

    Reactance (Xc): A measure of a materials ability to slow an

    electrical current.

    Therefore, the equation for Impedance is:

    22XcRZ

    Bi l t i l I d A l i

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    Bioelectrical Impedance Analysis

    Principle (contd):

    A BIA analyzer sends a weak electrical current via two

    electrodes through the body. That current is then detected

    by two other electrodes. The extent to which the electrical

    current is impeded or resisted is related to the type andamount of tissue through which it passes.

    Water is a very good conductor of electricity and has a very

    low level of resistance. Adipose tissue has a very low water

    content (~20%) and thus has a high level of resistance orimpedance. Muscle has a very high water content (~75%)

    and thus has a low level of resistance or impedance.

    Bi l t i l I d A l i

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    Bioelectrical Impedance Analysis

    Principle (contd):

    Essentially, BIA measures an individuals total body water

    based on the principle of the following equation:

    Volume = p x L2 / Z

    Where:

    p = a constant representing a materials ability to impede an

    electrical current at a given temperature

    L = Length (or in the case of humans, height)

    Z = Impedance

    Finally, since the approximate water content of adiposetissue and fat-free mass (muscle, bone, fluid, etc.) are

    known, the measurement of total body water can be

    converted to fat-free mass and then fat-mass and % body fat

    can be calculated.

    Bi l t i l I d A l i

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    Bioelectrical Impedance Analysis

    Bioelectrical Impedance Anal sis

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    Bioelectrical Impedance Analysis

    Bioelectrical Impedance Analysis

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    Bioelectrical Impedance Analysis

    BIA Procedures

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    BIA Procedures

    Conditions: No eating or drinking within 4 hours of the test

    No exercise within 12 hours of the test

    Urinate completely within 30 minutes of the test No alcohol consumption in the previous 48 hours

    No diuretics in the previous 7 days

    Limited use of diuretic agents before the test

    BIA Procedures

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    BIA Procedures

    Pre-Test Procedures: Record age, gender, height, weight, activity level, frame

    size

    Performing the test: Calibrate the instrument

    Prepare the subject for the test by having them liedown on the table Have subject remove all jewelry, right sock and shoe

    Arms and legs should not touch each other

    Clean electrode sites with alcohol pad, allow to dry Attach electrodes and wires

    Allow values to stabilize (~30 seconds)

    Enter information into BIA software

    BIA Procedures

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    BIA Procedures

    BIA Procedures

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    BIA Procedures

    Summary of Methods

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    Summary of Methods

    Interpretation of Data

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    Interpretation of Data

    Based on BMI:

    Interpretation of Data

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    Interpretation of Data

    Based on Circumferences:

    Pre- to Post-Changes

    Waist Circumference

    Waist-to-Hip Ratio

    WHR

    Waist

    Circumference

    Interpretation of Data

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    Interpretation of Data

    Based on Skinfolds, BIA, any technique thatprovides a calculation of %BF

    Pre- to Post-Changes

    Population Norms Calculations of Fat-Weight, Fat-Free Weight, Ideal

    Body Weight

    Interpretation of Data

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    Interpretation of Data

    Interpretation of Data

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    Interpretation of Data

    % BF Population Norms See previous slide

    % BF considered satisfactory for health:

    Men: 10 to 22% Women: 20 to 32%

    Interpretation of Data

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    Interpretation of Data

    Fat Weight: FW = BW x % BF

    % BF needs to be in decimal form (i.e. 25% = 0.25)

    Fat-Free Weight: FFW = BW x (1-%BF)

    OR FFW = BW - FW

    % BF needs to be in decimal form Ideal Body Weight:

    IBW = FFW / (1 Desired %BF)

    Desired %BF must be in decimal form

    Interpretation of Data

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    Interpretation of Data

    Ideal Body Weight (contd) How do you pick a desired %BF?

    Use population norm charts

    Based on attainable or incremental goals

    Based on %BF considered satisfactory for health

    Laboratory Exercise

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    Laboratory Exercise

    Procedures: Each student will collect the following data on him or

    herself using the following procedures: BMI, Skinfolds, Circumferences, BIA

    At least six students (hopefully more) will undergo

    underwater weighing Research Question

    Is there a statistically significant difference in %BF, FW,FFW between the three methods:

    UWW vs. Skinfolds UWW vs. BIA

    Skinfolds vs. BIA

    What is the correlation between the three methods forthese same variables?