Gleb Sukhorukov

/Queen Mary University of London, United Kingdom/

Micro- and NanoencapsulationLaboratory

St. Petersburg State Polytechnic University

Technology of Layer –by-Layer encapsulation (Max Planck Institute of Colloids and Interfaces)

- >25 groups working in area of LbL encapsulations in Europe, USA, China, Japan, Singapore, Australia and worldwide

- ~ 3,000 publications over 10 years

- Reserach impact (high citation numbers)

- Versatile applications (Biomedical, Pharma, Cosmetic, Food, Materials)

- Strong industrial interest (Bayer AG, BASF, L‘Oreal, Nestle...)

- Spin-off company Capsulution AG

Multifunctionality to Microcapsules (50nm – 50 Multifunctionality to Microcapsules (50nm – 50 mkm)mkm)

Macromolecule encapsulation

Macromolecule encapsulation

Cell-/Tissue-Targeting

Cell-/Tissue-Targeting

Visualization, Sensors

Visualization, Sensors

Controlled release

Controlled release Remote activationRemote activation

A+B

C

MicroreactorsMicroreactors

MagnetismMagnetism

ProtectionProtection

Most of activities are in:-Novel Phys/Chem properties;-Deliver systems;-Cell biology tool

Delivering/Sensing Submarine

Floating in body Submarine

External triggering / reporting inside cells/tissues

Perspective Research Activities at SPbSPU

Single-cell biopsy/ Cell-to Cell communicationSingle-cell biopsy/ Cell-to Cell communication Capsule-Surface interactionsCapsule-Surface interactions Fabrication of Release control over depot-systemsFabrication of Release control over depot-systems In vivo studies for encapsulated drugs deliveryIn vivo studies for encapsulated drugs delivery and more…and more…

- BioMedical & BioPhysics

Single-cell biopsy / Cell-to-Cell Communication

Capturing molecules intracellularly and delivery to other cells – artificial exosomes exchange

Available expertise: Mass-Spec, Cell facilities

Collaboration: Prof. M.KhodorkovskiiDr. Irina Nazarenko (Freiburg), Dr. Arkady Shipuline

Capsule – Surface Interaction- One of major problems of LbL microencapsulation technology – aggregation and sticking One of major problems of LbL microencapsulation technology – aggregation and sticking

to cell membranes when used in vivo.to cell membranes when used in vivo.- Some coatings (PLL-g-PEG, Dextran) are proposed to cope with the problem, but are not Some coatings (PLL-g-PEG, Dextran) are proposed to cope with the problem, but are not

absolute solution.absolute solution.

Study adhesion aiming to optimize selectivity to cell surface

Optical tweezers technique (M. Khodorkovski’s group) to measure

interaction forces.

Fabrication and Release control of depot-systems

Fabrication and controlled gradual release from microcapsules and its assemblies as a depot-system.

Monitoring on cell culture/ Tissue scaffolds

.Modelling of Release kinetics

We open for New Ideas on Other Areas!

Animal Testing and More……

Partner for sample testing -

St. Petersburg Institute of Vaccines and Sera

Russian Ministry of Health

Outlook / Key Performance Indicators

In 2-3 years time:

- 3-4 academic staff/postdocs, PhD students, master students

Publications (IF>3):

-1-2 (2015), 3-4 (2016, 2017..... and beyond)

Indusrial contracts and/or joint research grant (1-2 per year)

Research grants (RSF, ....EU, International) – 1 per year from 2016