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CurriculumVitae TereseM.Bergfors(néeHirth)

Dept.ofCellandMolecularBiology,BiomedicalCenter,Box596UppsalaUniversity,SE‐75124Uppsala,SwedenDateofBirth:January29,1955Telephone:+46(18)471‐4543Fax:+46(18)536971E‐mail:terese@xray.bmc.uu.seURL:http://xray.bmc.uu.se/terese SynopsisMy area of expertise is protein crystallization. I am part of the structural biologygroup of Prof. Alwyn Jones at Uppsala University where I have headed thecrystallizationactivitiessince1984. AsoneoftheeightPrincipal InvestigatorsonagrantfundedbytheSwedishFoundationforStrategicResearch,Iexpress,purify,andcrystallize tuberculosis proteins and work on the development of crystallizationmethodology.Education 1974B.A.inNaturalSciences,BellarmineCollege,LouisvilleKentucky,

USAEmploymentHistorySince1992 Department of Cell and Molecular Biology, Biomedical Center, Box

596,UppsalaUniversity,Sweden(HeadResearchEngineer)1984‐1991 DepartmentofMolecularBiology,BiomedicalCenterBox590,

Uppsala University,Sweden1982‐1984 CellMotilityGroup,StockholmUniversity,Sweden1980‐1981 DepartmentofPathology,UppsalaUniversity,Sweden1977‐1980 Biology Department, Southern Methodist University, Dallas Texas,

USA Consultancies2007 Microlytic,A/S;www.microlytic.com1992‐1996 AstraHässle,Mölndal,SwedenOther NovozymesA/S,Denmark;KaroBio,Stockholm;Bioventia,Uppsala.ProfessionalActivitiesSince2006 Co‐editor,ActaCrystallographicaFSince2004 Scientific Advisory Board, International Organization for Biological

Crystallization(IOBC)2007 GuestCo‐editor,ICCBM11Proceedings,JournalofCrystalGrowth

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2007 Thesisdefensecommittee:MichaelNielsen,“Expression,Purificationand Characterization of Tryptophan Hydroxylases”, Dept. ofChemistry,TheTechnicalUniversityofDenmark.

2005 Thesisdefensecommittee“Cristalizacióndeproteínasdeuncampoeléctricointerno”NuritMirkin,Dept.ofChemistry,UNAM,MexicoCity.

PublicationsBooksBergfors, T., Editor. Protein Crystallization: Second Edition. 2009. InternationalUniversityLine,LaJolla,California,500pp.Bergfors,T.,Editor.ProteinCrystallization:Techniques,Strategies,andTips.1999.InternationalUniversityLine,LaJolla,California,300pp.Journalarticlesorbookchapters20081. Bergfors, T. The RAPID crystallization strategy for structure‐based inhibitor

design. Integrating Crystallography in Drug Discovery. Editor: Sussman, J.SpringerPress,1‐10.

2. Ericsson,D.,Kasrayan,A.,Johansson,P.,Bergfors,T.,Sandström,A,Bäckvall,J‐E,Mowbray, S. X‐ray structure ofCandida antarctica lipase A shows a novel lidstructureandalikelymodeofinterfacialactivation.J.Mol.Biol.376,109‐119.

3. Covarrubias, A. S., Högbom, M., Bergfors, T., Carroll, P., Mannerstedt, K.,

Oscarson,S.,Parish,T., Jones,T.A.,Mowbray,S.Structural,biochemical,and invivo investigationsof thethreoninesynthase fromMycobacteriumtuberculosis.J.Mol.Biol.381,622‐633.

20074. Ubhayasekera,W.,Tang,C.M.,Ho,S.,Berglund,G.,Bergfors,T.,Chye,M‐L,and

Mowbray, S. Crystal structures of a family 19 chitinase from Brassica junceashowflexibilityofbindingcleftloops.FEBSLetters.274,3695‐3703.

5. Bergfors,T.Automatedliquid‐handlingsystemsforsubmicrolitercrystallization.

Protein Crystallization for Structural Genomics. Ed. Chayen, N. InternationalUniversityLine,LaJolla,California.

6. Bergfors,T.Succeedingwithseeding:somepracticaladvice.EvolvingMethods

forMacromolecularCrystallography. Editors:Read,R.&Sussman, J. SpringerPress,1‐10.

20067. Bergfors, T. Crystallization strategy at the Uppsala University RAPID Center.

SynchrotronRadiationScience&Technology13,5‐9.

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8. Bergfors, T. Screening and optimization methods for non‐automatedcrystallizationlaboratories.MethodsMol.Biol.363,131‐151.

20059. Covarrubias,A.,Larsson,A.,Högbom,M.,Lindberg,J.,Bergfors,T.,Björkelid,C.,

Mowbray, S., Unge, T. and Jones, T.A. Structure and function of carbonicanhydrassesfromMycobacteriumtuberculosis.J.Biol.Chem.280,18782‐18789.

10. Johansson, P., Unge, T., Cronin, A., Arand, M., Bergfors, T., Jones, T.A. and

Mowbray, S. Structure of an atypical epoxide hydrolase fromMycobacteriumtuberculosisgivesinsightsintoitsfunction.J.Mol.Biol.351,1048‐1056.

11. Larsson,A.,Bergfors,T.,Dultz,E., Irwin,D.,Roos,A.,Driguez,H,Wilson,D.and

Jones, T.A. Crystal structure of Thermobifida fusca endoglucanase Cel6A incomplexwithsubstrateand inhibitor: the roleof tyrosineY73 in substrate ringdistortion.Biochemistry44,12915‐12922.

200412. Roos,A.,Anderson,C.E.,Bergfors, T., Jacobsson,M,Karlen,A,Unge, T., Jones,

T.A., and Mowbray, S. Mycobacterium tuberculosis ribose‐5‐phosphateisomerasehasaknownfold,butanovelactivesite.J.Mol.Biol.335,799‐809.

200313. Bergfors,T.SeedstoCrystals.J.Struct.Biol.142,66‐76.14. Johansson, P. Denman, S., Brumer, H., Kallas, Å., Henriksson, H., Bergfors, T.,

Teeri, T., and Jones, T.A. Crystallization and preliminary X‐ray analysis of axyloglucanendotransglycosylasefromPopulustremulaxtremuloides.ActaCrystD59,535‐537.

15. Jakobsson,E.,Alvite,G.,Bergfors,T., Esteves,A., andKleywegt,G. Thecrystal

structureofEchinococcusgranulosus fatty‐acid‐bindingprotein1.BBA‐ProteinsandProteonomics49,40‐50.

16. Arand,M.,Hallberg,B.,Zou,J.,Bergfors,T.,Oesch,F.,vanderWerf,M.,deBont,

J., Jones, T. & Mowbray, S. 1.2 Å structure of Rhodococcus erythropolislimonene‐1,2‐epoxidehydrolaserevealsanovelmechanism.EMBOJ.22,2583‐2592.

17. Arand,M.,Cronin,A.,Oesch,F.,Hallberg,M.,Zou,JY,Bergfors,T.,Mowbray,S.,

andJones,T.A.Thetell‐talestructuresofepoxidehydrolases.DrugMetabolismReviews35:7Suppl.1.

2002‐198418. Van Aalten, D.M.F.,Milne, K.G., Zou, J.Y., Kleijwegt, G., Bergfors, T., Ferguson,

M.A.J.,Knudsen,J.andJones,T.A.Bindingsitedifferencesrevealedbycrystal

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structures of Plasmodium falciparum and bovine Acyl‐CoA binding protein. J.Mol.Biol.(2001)309,181‐192.

19. Zou,J.,Hallberg,B.M.,Bergfors,T.,Oesch,F.,Arand,M.,Mowbray,S.andJones,

T.A. Structure of Aspergillus niger epoxide hydrolase at 1.8Å resolution:implicationsforthestructureandfunctionofthemammalianmicrosomalclassofepoxidehydrolases.Structure(2000)8,111‐122.

20. Hallberg, B.M., Bergfors, T., Backbro, K., Pettersson, G., Henriksson, G., and

Divne, C. A new scaffold for binding haem in the cytochrome domain of theextracellularflavocytochromecellobiosedehydrogenase.Structure(2000)8,79‐88.

21. Chaudhuri,B.N.,Kleywegt,G.J.,Broutin‐L'Hermite, I., Bergfors,T., Senn,H., Le

Motte, P., Partouche, O., and Jones, T.A. Structures of cellular retinoic acidbindingproteinsIandII incomplexwithsyntheticretinoids. ActaCryst.(1999)D55,1850‐1857.

22. Kleywegt,G.J.,Bergfors,T.,Senn,H.,LeMotte,P.,Gsell,B.,Shudo,K.,andJones,

T.A. Crystal structures of cellular retinoid acid binding proteins I and II incomplexwith all‐trans retinoic acid and a synthetic retinoid. Structure (1994)2:1241‐1258.

23. Bergfors,T.,Kleywegt,G.J.,andJones,T.A. CrystallizationandX‐rayanalysisof

recombinant bovine cellular retinoic acid binding protein. Acta Cryst. (1994)D50,370‐374.

24. Uppenberg, J., Patkar, S., Bergfors, T., and Jones, T.A. Crystallization and

preliminary studies of lipase B fromCandida antarctica . J.Mol. Biol. (1994)235,790‐792.

25. Kuehn, M.J., Ogg, D.J., Kihlberg, J., Slonim, L. Flemmer, K., Bergfors, T., and

Hultgren,S.StructuralbasisofpilussubunitrecognitionbythePapDchaperone.Science(1993)272,1234‐1241.

26. Rouvinen,J.,Bergfors,T.,Teeri,T.andJones,A.Thethreedimensionalstructure

ofthecoreproteinofcellobiohydrolaseII.Science(1990)249,380‐386.27. Jones,T.A.,Cowan,S.,Newcomer,M.andBergfors,T. Crystallographicstudies

ontwofamiliesofretinoidbindingproteins.FrontiersinDrugResearch,AlfredBenzonSymposium28,Munksgaard,Cophenhagen.(1990).

28. Cowan, S., Bergfors, T., Jones, T.A., Tibbelin, G., Olin, B., Board, P., and

Mannervik, B. Crystallization of GST2, a human class alpha glutathionetransferase.J.Mol.Biol.(1989)208,369‐370.

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29. Bergfors,T.,Rouvinen, J., Lehtovaara,P.,Caldentey,X.,Tomme,P.,Claeyssens,M.,Pettersson,G.,Teeri,T.,Knowles,J.andJones,T.A.CrystallizationofthecoreproteinofcellobiohydrolaseIIfromTrichodermareesei.J.Mol.Biol.(1989)209,167‐169.

30. Jones,T.A.,Bergfors,T.,Sedzik,J.andUnge,T.Thethreedimensionalstructure

ofP2myelinprotein.EMBOJ.(1988)7,1597‐1604.31. Sedzik,J.,Bergfors,T.,Jones,T.A.andWeise,M.BovineP2myelinbasicprotein

crystallizesinthreedifferentforms.J.Neurochem.(1988)50,1908‐1913.32. Bergfors, T., Sedzik, J., Unge, T., Fridborg, K., Weise, M. and Jones, T.A.

CrystallizationofP2myelinprotein.J.Mol.Biol.(1987)198,357‐358.33. Hultcrantz,R.,Ericsson,J.andHirth,T.Levelsofmalondialdehydeproductionin

ratliverfollowingloadingandunloadingwithiron.VirchowsArch(CellPathol)(1984)45,135‐146.

34. Abok, K.,Hirth, T., Ericsson, J., andBrunk,U. Effect of iron on the stability of

macrophagelysosomes.VirchowsArch(CellPathol)(1983)43,85‐101.Chairmanshipatinternationalconferences

• 2007 Co‐chair, Microsymposium on Protein Crystallization, 24th EuropeanCrystallizationMeeting,Marrakkesh,Morocco

• 2004 Sessionchair,Tenth InternationalConferenceon theCrystallizationofBiologicalMacromoleculesCrystallizationCourse,Bejing,China

• 2003 Chairman of the Microsymposium on Protein Crystallization, 21stEuropeanCrystallizationMeeting,Durban,SouthAfrica

Acceptedinvitationsasaspeaker(internationalvenues)2008

• ICCBM12(InternationalWorkshopandConferenceontheCrystallizationofBiologicalMacromolecules12),Cancun,Mexico

• InternationalSchoolofCrystallography,Erice,Italy• IUCr,InternationalUnionofCrystallographyXXIIIWorldCongress,Osaka,

Japan• Dept.ofChemistry,UniversityofTokyo,Tokyo,Japan• PEPC6(ProteinExpression,Purification,&Crystallization6),EMBOCourse,

Hamburg,Germany• AdvancedMethodsinCrystallizationIII,FEBSAdvancedCourse(Federation

ofBiologicalSciences),NoveHrady,CzechRepublic• AustralianCourseinMacromolecularCrystallization,CSIRO(Commonwealth

ScientificandIndustrialResearchOrganization),Melbourne,Australia.• ProteinCrystallizationSymposium,InstituteforMolecularBioscience,

UniversityofQueensland,Brisbane,Australia

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2007

• XIVCongresoIbéroamericanodeCristalografía,Caracas,Venezuela• ProteasePlatform,Novartis,Basel,Switzerland• Hauptmann‐Woodward Medical Research Institute Lecture Series, Buffalo,

NY• 14thCCP4NorthProteinStructureWorkshop,Carlisle,UK

2006

• UniversityofCopenhagen,Dept.ofChemistry• 11th International Conference on the Crystallization of Biological

MacromoleculesCrystallizationCourse,Quebec,CanadaPLENARYSPEAKER• British Crystallographic Association SpringMeeting,University of Lancaster,

UK• CentroNacionaldeInvestigacionesOncológicas(CNIO),Madrid,Spain• InternationalSchoolonBiologicalCrystallization,Granada,Spain• FEBS Course Advanced Methods in Macromolecular Crystallization, Nove

Hrady,CzechRepublic.• EMBO Practical Course in: Protein Expression, Purification, and

Crystallization(PEPC5),EMBL,Hamburg,Germany.

2005• Advances inProteinScienceMeeting,TheChineseUniversityofHongKong,

China.• DepartmentofChemistry,UNAM,MexicoCity,Mexico.• EvolvingMethodsinMacromolecularCrystallography,InternationalSchoolof

Crystallography,Erice,Sicily,Italy.PLENARYSPEAKER

2004• FEBS Course Advanced Methods in Macromolecular Crystallization, Nove

Hrady,CzechRepublic.• EMBO Practical Course in: Protein Expression, Purification, and

Crystallization(PEPC4),EMBL,Hamburg,Germany.• Tenth International Conference on the Crystallization of Biological

MacromoleculesCrystallizationCourse,Bejing,China.

2003• KeynotespeakerattheSixthHeartofEuropeanbio‐CrystallographyMeeting,

Wittenberg,Germany.• AmericanCrystallographicAssociationAnnualMeeting,Cincinnati,OhioUSA.

2002

• EMBO Practical Course in: Protein Expression, Purification, andCrystallization(PEPC3),EMBL,Hamburg,Germany.

• Ninth International Conference on the Crystallization of BiologicalMacromoleculesCrystallizationCourse,JenaGermany.

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2001• RecentAdvancesinMacromolecularCrystallization,LeBischenberg,France.• First Southeast Asian Protein Crystallography Workshop, Suranaree

UniversityofTechnology,NakhonRatchasima,Thailand

2000andpreviousyears• 2000 EMBO course in Protein Expression, Purification, and Crystallization,

Hamburg,Germany• 1999XVIIIMeetingof the InternationalUnionofCrystallography,Glasgow,

Scotland• l997 Recent Advances in Macromolecular Crystallization, Le Bischenberg,

France• 1994 Workshop on Protein Crystallization, Dept. of Organic Chemistry,

TechnicalUniversity,Budapest,Hungary• 1993 Fifth International Conference on the Crystallization of Biological

Macromolecules,SanDiego,USATeachingOrganizerofyearlygraduatelevelcourses

• PracticalProteinCrystallizationGraduateCourse,UppsalaUniversity• WritingaScientificJournalArticleinEnglish,UppsalaUniversity

LecturerforothercoursesinScandinavia• Crystallography Course, Dept. of Chemistry, University of Copenhagen,

Denmark.• Protein Structures Course,UmeåCenter forMolecular Pathogenesis,Umeå

University,Sweden.• Protein Technology Course, Swedish Agricultural University, Uppsala,

Sweden.• Advanced Course in Structural Biochemistry and Bioinformatics, Stockholm

University,Sweden.Prizes2001 "Best practical crystallization tip" at Recent Advances in

MacromolecularCrystallization,LeBischenbergFrance.1997 “Best oral presentation” at Recent Advances in Macromolecular

Crystallization,LeBischenbergFrance.1997 “Best practical crystallization tip” at Recent Advances in

MacromolecularCrystallization,LeBischenbergFrance.