PROTEIN CRYSTALLIZATION - Uppsala Universityxray.bmc.uu.se/~terese/files/Protein...
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International University Line P.O.Box 2525, La Jolla, California, 92038 TEL: (858) 457-0595 • FAX: (858) 581-9073 • EMAIL [email protected] PROTEIN CRYSTALLIZATION Second Edition edited by Terese Bergfors 500 p. — (IUL Biotechnology series 1a) illustrated, hardcover, $109.95 ISBN: 9780972077446 Completely revised and updated, Protein Crystallization, 2 nd Edition is a greatly expanded follow-up to the best-selling 1 st edition. Completely new chapters on high-throughput methods, mass spectrometry, microcalorimetry, counterdiffusion, heavy- atom derivatization, selenomethionine-labeling, rational strategies for crystallization, and protein modification to improve crystallization. • Updated chapters on formulation of the protein before crystallization, characterization of the protein by dynamic light scattering, classic methods and the phase diagram, seeding, and cryoprotection of the crystals. • Thirty full-color plates for evaluating crystallization drops. • Separate section of laboratory exercises, ideal for crystallization courses. • A-Z glossary. “Why is crystallization important? At different times, different aspects of protein crystallography have assumed the role of bottleneck. Thus at one time the bottleneck consisted of the lack of a methodology for structure determination, even if a sufficient number of diffraction intensities were available for structure solution. With the advent of powerful methods for structure solution, the bottleneck shifted to the difficulty in obtaining crystals in sufficient number and of suitable quality to do the diffraction experiment. The present book is dedicated to the problem of describing current methods for protein crystallization and describing them as clear laboratory protocols.” Dr. Herbert A. Hauptman, Nobel Laureate in Chemistry “This is an essential handbook for anyone engaged in crystallization of macromolecules. It is exceptionally well organized and illustrated and has contributions from all the leaders in what continues to be a challenging and critically important field.” Dr. William L. Duax, American Crystallographic Association, Executive Officer Contents Part I. METHODS 1. Some Words of Advice from an Old Hand Alexander McPherson 2. Rational Selection of Crystallization Techniques Joseph R. Luft and George T. DeTitta 3. Automation for Crystallization: Practical Considerations in Choosing a System Joseph R. Luft and George T. DeTitta 4. Oils for Screening and Optimization Naomi E. Chayen 5. Crystallization in Gels and Capillaries Juan M. García-Ruiz 6. Seeding Aengus Mac Sweeney and Allan D’Arcy 7. Heavy-Atom Derivatization Zbigniew Dauter and Mirosława Dauter 8. Preparation and Crystallization of Selenomethionine Protein Anna M. Larsson 9. Crystal Handling for Cryogenic Data Collection Elspeth F. Garman PART II. TOOLS and STRATEGIES 10. Interpretation of the Crystallization Drop Results Johan Philip Zeelen 11. Mass Spectrometry Applications in Protein Crystallography Sharon X. Gao and Marie Zhang 12. Dynamic Light Scattering Ulf Nobbmann and Terese Bergfors 13. Improving Protein Crystallizability by Modifications and Engineering Xiayang Qiu and Cheryl A. Janson 14. Handling the Protein Sample Terese Bergfors 15. Two Approaches for Initial Screening: Evolution and Intelligent Design Janet Newman 16. Strategies for Protein Crystallization Screening Bernhard Rupp 17. Additives and Microcalorimetric Approaches for Optimization of Crystallization Joanne I. Yeh PART III. LABORATORY EXERCISES PART IV. A–Z Terese Bergfors
Transcript of PROTEIN CRYSTALLIZATION - Uppsala Universityxray.bmc.uu.se/~terese/files/Protein...
International University Line P.O.Box 2525, La Jolla, California, 92038 TEL: (858) 457-0595 • FAX: (858) 581-9073 • EMAIL [email protected]
PROTEINCRYSTALLIZATIONSecondEdition
editedby
TereseBergfors500p.—(IULBiotechnologyseries1a)illustrated,hardcover,$109.95ISBN:9780972077446
Completely revised and updated, Protein Crystallization, 2nd Edition is a greatly expanded follow-up to the best-selling 1st edition. Completely new chapters on high-throughput methods, mass spectrometry, microcalorimetry, counterdiffusion, heavy-atom derivatization, selenomethionine-labeling, rational strategies for crystallization, and protein modification to improve crystallization.
• Updated chapters on formulation of the protein before crystallization, characterization of the protein by dynamic light scattering, classic methods and the phase diagram, seeding, and cryoprotection of the crystals.
• Thirty full-color plates for evaluating crystallization drops.
• Separate section of laboratory exercises, ideal for crystallization courses.
• A-Z glossary. “Why is crystallization important? At different times, different aspects of protein crystallography have assumed the role of bottleneck. Thus at one time the bottleneck consisted of the
lack of a methodology for structure determination, even if a sufficient number of diffraction intensities were available for structure solution. With the advent of powerful methods for structure solution, the bottleneck shifted to the difficulty in obtaining crystals in sufficient number and of suitable quality to do the diffraction experiment. The present book is dedicated to the problem of describing current methods for protein crystallization and describing them as clear laboratory protocols.”
Dr. Herbert A. Hauptman, Nobel Laureate in Chemistry “This is an essential handbook for anyone engaged in crystallization of macromolecules. It is exceptionally well organized and illustrated and has contributions from all the leaders in what continues to be a challenging and critically important field.”
Dr. William L. Duax, American Crystallographic Association, Executive Officer
Contents
Part I. METHODS 1. Some Words of Advice from an Old Hand
Alexander McPherson 2. Rational Selection of Crystallization Techniques
Joseph R. Luft and George T. DeTitta 3. Automation for Crystallization: Practical Considerations in
Choosing a System Joseph R. Luft and George T. DeTitta
4. Oils for Screening and Optimization Naomi E. Chayen
5. Crystallization in Gels and Capillaries Juan M. García-Ruiz
6. Seeding Aengus Mac Sweeney and Allan D’Arcy
7. Heavy-Atom Derivatization Zbigniew Dauter and Mirosława Dauter
8. Preparation and Crystallization of Selenomethionine Protein Anna M. Larsson
9. Crystal Handling for Cryogenic Data Collection Elspeth F. Garman PART II. TOOLS and STRATEGIES
10. Interpretation of the Crystallization Drop Results Johan Philip Zeelen
11. Mass Spectrometry Applications in Protein Crystallography Sharon X. Gao and Marie Zhang
12. Dynamic Light Scattering Ulf Nobbmann and Terese Bergfors
13. Improving Protein Crystallizability by Modifications and Engineering Xiayang Qiu and Cheryl A. Janson
14. Handling the Protein Sample Terese Bergfors
15. Two Approaches for Initial Screening: Evolution and Intelligent Design Janet Newman
16. Strategies for Protein Crystallization Screening Bernhard Rupp
17. Additives and Microcalorimetric Approaches for Optimization of Crystallization Joanne I. Yeh
PART III. LABORATORY EXERCISES PART IV. A–Z
Terese Bergfors
