1. Dr. Vijay Marakala, MBBS, MD. Assistant professor BIOCHEMISTRY SIMS & RC

2. No vegetable oil contains any cholesterol. Cholesterol is the major sterol in animal tissues. Only a little portion of the body cholesterol is derived from diet. The bulk of it is synthesized in the body. Unsaturated fatty acids lower blood cholesterol levels by controlling cholesterols synthesis as well as its elimination from the body. 3. Cyclopentano perhydro phenanthrene ring. Total 27 carbon atoms. One hydroxyl group at the third position. Double bond between carbon atom 5 and 6 An 8 carbon side chain at position 17 Methyl side chains at positions 10 and 13 4. 1. Major structural constituent of the cell membranes and lipoproteins 2. Precursor for Steroid hormones a. Progesterons b. Glucocorticoids c. Mineralocorticoids d. Androgens & Estrogens 3. Formation of Bile acids and bile salts. 4. Synthesis of Vitamin D 5. Lipids Proteins LIPOPROTEINS 6. CHOLESTEROL ESTER TRIACYLGLYCEROL PHOSPHOLIPID FREE CHOLESTEROL APOPROTEIN 7. LIPOPROTEIN 8. LIPOPROTEIN CM VLDL IDL LDL HDL 9. HDLVLDLLDL Origin HDL VLDL LDL CHYLOLICRON ELECTROPHORESIS ULTRACENTRIFIGATION LIPOPROTEIN 10. PROTEIN COMPONENT OF LIPOPROTEIN PROMOTE TRANSPORT AND UPTAKE OF LIPIDS ACTS AS LIGAND ACTIVATION OF ENZYMES APOPROTEINS 11. C-I C-II C-III Except LDL B-48 B-100 A-I A-II A B CE 12. All liver Liver B-48: Intestine B-100: Liver A-I: Liver & Intestine A-II:Liver A B CE 13. CHYLOMICRON Formed in the intestinal mucosal cells Rich in TG Contain apo-B-48 Apo-E Apo-C apo-A 14. VLDL Synthesized in the liver Contain apo-B-100, C-II and apo-E 15. IDL Contain apoB-100 and Apo-E 16. LDL Contain apoB-100 17. HDL Contain Apo-A-I[Major] Apo-C Apo-E 18. LDL- Highest CE HDL Highest protein CM Highest TG 19. ANALYTE NORMAL VALUE Total plasma lipids 400-600mg/dl Total cholesterol 140-200mg/dl HDL cholesterol 4060 mg/dL LDL cholesterol 80-130mg/dl Triglycerides 50-150mg/dl