1.Dr Vijay Marakala MBBS.,MD. Assistant professor Biochemistry SIMS&RC

2. How fats are transported? From intestine From liver and adipose tissue What is lipoprotein? Types Apoprotein Metabolism Enzymes Proteins 3. VARIOUS TISSUE 4. Lipids Proteins LIPOPROTEINS 5. CHOLESTEROL ESTER TRIACYLGLYCEROL PHOSPHOLIPID FREE CHOLESTEROL APOPROTEIN 6. LIPOPROTEIN 7. LIPOPROTEIN CM VLDL IDL LDL HDL 8. HDLVLDLLDL Origin HDL VLDL LDL CHYLOLICRON ELECTROPHORESIS ULTRACENTRIFIGATION LIPOPROTEIN 9. PROTEIN COMPONENT OF LIPOPROTEIN PROMOTE TRANSPORT AND UPTAKE OF LIPIDS ACTS AS LIGAND ACTIVATION OF ENZYMES APOPROTEINS 10. C-I C-II C-III Except LDL B-48 B-100 A-I A-II A B CE 11. All liver Liver B-48: Intestine B-100: Liver A-I: Liver & Intestine A-II:Liver A B CE 12. CHYLOMICRON Formed in the intestinal mucosal cells Rich in TG Contain apo-B-48 Apo-E Apo-C apo-A 13. VLDL Synthesized in the liver Contain apo-B-100, C-II and apo-E 14. IDL Contain apoB-100 and Apo-E 15. LDL Contain apoB-100 16. HDL Contain Apo-A-I[Major] Apo-C Apo-E 17. LDL- Highest CE HDL Highest protein CM Highest TG 18. ANALYTE NORMAL VALUE Total plasma lipids 400-600mg/dl Total cholesterol 140-200mg/dl HDL cholesterol 4060 mg/dL LDL cholesterol 80-130mg/dl Triglycerides 50-150mg/dl 19. Requirements ENZYMES TRANSFER PROTEINS 20. ENZYMES and TRANSFER PROTEIN Lipoprotein lipase[LPL] Lecithin: Cholesterol acyl transferase [LCAT] Hepatic lipase Cholesterol ester transfer protein [CETP] 21. LPL LIPOPROTEIN LIPASE Apo C-II + 22. LCAT