Are you multiplexing using multiple dyes or using flow ... · provide custom solutions for your...
Transcript of Are you multiplexing using multiple dyes or using flow ... · provide custom solutions for your...
Advance your discovery &development programs
Advanced Cellular Analysis Solutions
the full spectrum ofFlow Cytometry
Custom Design Services
Do you want stable, dry reagentsthat can be stored at roomtemperature with little tube totube variation?
Are you multiplexing using multiple dyes orusing flow cytometry to analyze a single cell?
Do you need a custom antibody conjugation or are you looking for a different fluorochrome conjugate?
CDS: Custom Design ServicesConsider the convenience of combining the antibodies you know and trust withleading conjugates from BCI
For more than 75 years, Beckman Coulter has been providing researchers with high-quality
products and services. Our Custom Design Services (CDS) offer personalized options designed to
provide custom solutions for your multicolor flow cytometry and imaging needs.
As a recognized leader in multicolor flow cytometry, our CDS product options include purified
antibodies and single and multicolor antibody conjugates, complete with optimization services.
A wide selection of fluorochromes provides options to meet specific multicolor experimental design
requirements. We have an expanded CDS portfolio, offering multicolor cocktails in a dry,
temperature-stable format.
Our Custom Design Solution Service
Whether you are looking for a different conjugate of our antibody
or need a custom conjugation of your own antibody, CDS provides
affordable, high-quality custom antibody conjugation services.
Our CDS offerings range from custom conjugations to optimized
multi-color combinations.
In addition, our dry reagents, the DuraClone line, provide a means
to simplify and expedite sample preparation and handling. These
temperature-stable reagents allow for the production of a single
batch that can be stored at room temperature for the duration of a
study eliminating the need for refrigeration space and revalidation
of new lots. DuraClone custom panels are supplied in a single use
cocktail per tube ready for addition of sample.
Add bloodsample
Vortex &incubate
Add lysis solution& incubate
Acquire &analyze by flowcytometry
Take dryunitized dried-downreagent tube
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reagents: A2z
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Take dry unitizeddried-downreagent tube
Add bloodsample
Vortex &incubate
Add lysissolution &incubate
Acquire &analyze by flowcytometry
A wide selection of fluorochromes across BCI’s popular
antibody clones provides options to meet specific multicolor
experimental design requirements. With flexible order
quantities, we make custom antibody conjugates more
affordable allowing tight budgets to go further and
removing bottlenecks to your downstream work.
Antibodies may be lyophilized or supplied in a specified
buffer solution. Conjugates are suitable for flow cytometry,
UV-microscopy or chemiluminescent applications (ELISA,
Western blots).
Experts in Conjugation
BCI scientists have extensive experience in developing fluorescent,
antibody-based detection agents. The same scientists that have
developed our products t manufacture all custom reagents. Our scientists
have obtained patents on several processes, developed proprietary
processes for increased quality and collaborated with external research
groups in the design of complex studies that have been published in
peer-reviewed journals. We have expertise in Dye Technology, Conjugation,
Multicolor Design and Dry Reagent Processing.
Incorporate our portfolio of high-quality antibodies with custom
fluorochrome conjugates in a dry reagent format and you have
temperature-stable cocktails that can be quickly and reliably
incorporated into your laboratory protocols. We provide state-of-the-art
tools to advance discovery and development programs.
Fluorochrome & Conjugation TechnologiesBCI Tandems require less compensation
Beckman Coulter has set the standard for tandem dyes and we continue
to be unmatched in quality. We have developed proprietary technology to
assure maximal product performance. Our extensive expertise along with
our patented tandem dye manufacturing process, delivers the complete
solution to help expand your research needs.
Product Category Description Suggested Antibody Source Minimum Quantity
Custom Pacific Blue* 500 ug Conjugation of BCI owned antibodiesConjugations Krome Orange or customer-supplied antibodies Fluorescein PE PE tandem dyes: ECD, PC5, PC5.5, PC7 APC APC tandem dyes: APC-Alexa Fluor* 700, APC-Alexa Fluor 750 Biotin
Custom Dry Cocktails, 2-color 6-color 750 tests, Optimizations and configuration with DuraClone 3-color 7-color Single test tubes existing BCI-conjugated or 4-color 8-color customer-supplied antibodies 5-color 9-color 10-color
Custom Cocktails and 2-color 6-color 500 tests Optimizations and configuration withPanels 3-color 7-color existing BCI-conjugated or 4-color 8-color customer-supplied antibodies 5-color 9-color 10-color
Unlabeled Antibodies Available in liquid or lyophilized, 1 mg Concentration and buffer can be with or without sodium azide specified
Inquire for additional available dye conjugations not listed above.
Bring Beckman’s Design Expertise to YourResearch Needs
BCI has maintained a leadership position in the design and optimization
of multicolor reagent combinations and systems for research. Our extensive
expertise, along with our patented tandem dye manufacturing process
and dry reagents technology, delivers the complete solution to help
expand your research needs.
• Experts in routine designs of four-six color cocktails
• Experts in dry reagents technology
• Extensive experience of eight-10 color custom cocktail formulations
• “Backbone” strategies designed to simplify reagent drop-ins
• Wide variety of single color conjugates, including our exclusive Krome Orange dye
• Expertise with a range of washed and no-wash applications
Formulation & Packaging Technology
Our proprietary formulation provides superior binding specificity
and performance. Eliminating non-specific binding of fluorochromes to
monocytes improves the ability to resolve low density positive
staining, thus avoiding erroneous results and misinterpretation of the
data in studies involving non-lymphoid populations.
• Provides enhanced signal-to-noise
• Reduced non-specific binding of cyanine dyes to non-lymphoid populations
• Low background fluorescence on negative populations
Our proprietary packaging extends reagent shelf-life whether in wet or
dry format. Recurring light exposure of conventional vials with use, even
with sitting on the bench, can degrade the product over time and lead to
changes in performance that make reproducible studies more difficult.
DuraClone dry reagents provide a stable solution that can be stored at
room temperature. Compensation problems can be encountered when
using multiple reagents with different storage dates.
• Minimizes photo-oxidative degradation
• Maintains consistent energy transfer efficiency in tandem dyes
• Maintains consistent fluorescence intensity during storage and under experimental conditions
• Enables storage of cocktails at room temperature.
Advanced Cellular Analysis Solutions
the full spectrum ofFlow Cytometry
Custom Design Service products are for Research Use Only. Not for use in diagnostic procedures.
*Pacific Blue and Alexa Fluor are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. (Invitrogen Corp.).
Beckman Coulter and the stylized logo are trademarks of Beckman Coulter, Inc. and are registered with the USPTO.
BR-16217B B2011-12733 © 2013 Beckman Coulter, Inc. PRINTED IN U.S.A.
Custom Design Services
A Partnership That Works
We take care of the whole workflow, from optimal design to manufacturing. We work
with you to optimize your formulations. From synthesis, modification and labeling and
from conjugation to purification/analysis, we can do it all in house for you.
For further details, please visit www.BeckmanCustomDesign.comor email us at [email protected]
Warranty. BCI warrants that, for twelve (12) months from the date of manufacture by BCI, the above product will perform in accordance with the performance criteria in the product data
sheet when used and stored as set forth in the product data sheet. BCI’s sole obligation and Customer’s exclusive remedy if BCI fails to ship Products that comply with the foregoing
warranty shall be limited to replacing, as soon as is commercially reasonable for BCI, any such non-conforming Products or, at BCI’s election, refunding the amounts paid by Customer
for the nonconforming Products. No warranty claim may be made for a shipment of Products unless made within the warranty period. BCI may require that Product subject to a war-
ranty claim be returned for inspection and BCI may test performance of retained samples of Product to verify non-conformance. THERE ARE NO OTHER WARRANTIES UNDER THIS
AGREEMENT, EXPRESS OR IMPLIED, AND NONE SHALL BE CREATED, WHETHER UNDER THE UNIFORM COMMERCIAL CODE, THE COURSE OF DEALINGS BETWEEN THE PARTIES
OR CUSTOM OR USAGE IN THE TRADE OR INDUSTRY. BCI EXPRESSLY DISCLAIMS ANY EXPRESS OR IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR
PURPOSE. Customer expressly acknowledges that BCI has not made any recommendations, statements, representations or warranties as to the utility or efficacy of the Products.