BD FACSCalibur Flow Cytometer - Florida Atlantic Universitymed.fau.edu/calibur.pdf · BD...

BD FACSCalibur Flow CytometerThe Automated, Multicolor Flow Cytometry System

A Complete System for

Today’s Laboratory

BD BiosciencesClontechDiscovery LabwareImmunocytometry SystemsPharmingen

www.bdbiosciences.com

The BD FACSCalibur System:

The Gold Standard That Continues to Evolve

The BD FACSCalibur™ flow cytometer is another BD Biosciences first: a multicolor benchtop flow cytometry system that is capable of bothanalyzing and sorting. Combined with your choice of sample loader, a wide variety of reagents, and powerful software, the BD FACSCalibursystem offers the high throughput necessary to meet productivityrequirements without compromising the flexibility and performanceessential for today’s busy, cost-conscious laboratory.

1

The Gold Standard thatContinues to Evolve

Since 1974, when the first

Fluorescent Activated Cell

Sorter was introduced,

BD Biosciences has been

the leading provider

of innovative technology

and advanced solutions in

flow cytometry systems.

Recognized as the gold

standard in automated

benchtop sorting and dual-

laser technology, the

BD FACSCalibur™ system

continues to be the

instrument of choice for

research and clinical

laboratories worldwide.

Your investment in a

BD Biosciences instrument

pays off over and over again

because they provide

modular, flexible solutions

that can be adapted and

evolved as your needs

change. You have the option

to choose a basic system,

or complete, automated

solutions, or a variety of

choices in between. Most

important, you’ll always get

the industry’s most usable

software and the service and

support that come only from

BD Biosciences.

Compact and easy to use, the BD FACSCalibur system can quickly perform routinetasks including immunophenotyping, absolute counting, residual white blood cellenumeration, stem cell analysis, DNA analysis, and isolation by sorting. And with its superior multicolor analysis and sorting capabilities, the BD FACSCalibur system is a highly sensitive tool, providing laboratories with the technological capabilitiescrucial for sophisticated cellular and molecular work in areas such as genetics,proteomics, multicolor analysis, and immune function.

The BD FACSCalibur system.

The modular instrument of choice

for research and clinical laboratories

worldwide. The gold standard that

continues to evolve.

BD FA C S Calibur Flow Cytometer:

A Complete System For To d a y ’sL a b o r a t o r y

BD FA C S Calibur Cytometer*:

A Modular Benchtop System

The BD FA C S Calibur flow cytometer is the first automated multicolorflow cytometry system that performs

both analysis and sorting in onebenchtop system. Designed specifically

to support a wide range of applications,the BD FA C S Calibur flow cytometer is afully integrated multiparameter system

that ensures objective, reproducibleresults so vital in today’s clinical and

research laboratories.

The BD FA C S Calibur systems’ user-centered design approach goes beyondmere ergonomics. Software instrument

control, auto–sample loading, andpushbutton fluidic control maximize

ease-of-use and productivity. Simple yet powerful software allows you to customize applications while

automating redundant tasks. Finally,the BD FA C S Calibur system lets you

leverage your investment through a modular design that is flexible andupgradeable to meet your future needs.

BD FA C S L o a d e r*:

Routine Automation Enhances

P ro d u c t i v i t y

With the BD FA C S ™ Loader option,

the BD FA C S Calibur system provideswalkaway automation for routine

applications. Featuring removable 40-tube carousels, on-board mixing,and BD™ Wo r k l i s t M a n a g e r* software,

the BD FA C S Loader option off e r sunsurpassed flexibility to eff i c i e n t l y

address the workflow demands oft o d a y ’s laboratories.

BD Multiwell AutoSampler†:

Flexible Easy-to-Use Automation

For those who prefer to work with

multiwell plates, BD Biosciences off e r sthe first walkaway sample introductiondevice for 96- and 384-well plates, both

standard and deep-well. BD Multiwell™Plate Manager software provides

an easy-to-use interface for you toinput sample information and createcustomized protocols, making experi-

ment runs as simple as click-and-go.

Dual Lasers:

Optimized for Multicolor Analysis

The BD FA C S Calibur system offers the industry standard in multicolor

analysis capability—two lasers spatiallyseparated for high sensitivity, minimal

compensation, and maximum flexibilityin fluorochrome selection. With multi-color analysis, you maximize the

information gained from small samples,as well as increase the cost-eff e c t i v e n e s s

of each assay.

Cell Sorting†:

Innovative Technology Makes Cell

Sorting Routine

The BD FA C S Calibur system is the

first benchtop flow cytometry systemavailable that is capable of sorting

cells of interest for further study. With the BD FA C S Calibur system,sorting is routine—just gate the

population of interest and clickA C Q U I R E—the BD FA C S Calibur

system does the rest. Sorting occurs in a completely enclosed, aerosol-freeenvironment for enhanced safety when

processing biohazardous samples. Cellssorted by the BD FA C S Calibur system

are collected into test tubes, or ifdesired, can be deposited directly onto filters or cell culture inserts with

the Cell Concentrator Module option.

BD FA C Station

Data Management System:

Powerful and Easy-to-Use

The ease-of-use of the BD FA C S C a l i b u r

system is enhanced by its user- f r i e n d l ydata management system. The

BD FACStation™ workstation takesadvantage of the intuitive Macintosh®

environment by using simple pull-down

menus and icons. It performs all thecomputing tasks required for fast,

accurate results, including instrumentsetup as well as data collection,analysis, and management.

The BD FACStation workstation o ffers automated software and

high-performance acquisition andanalysis tools for plotting, gating,statistical analysis, and reporting.

3 www.bdbiosciences.com

FACSCalibur 8/22/02 5:46 PM

4www.bdbiosciences.com

Reagents

BD Biosciences offers a broad range of reagent products designed to meet multipleapplication requirements— from reagent and software systems for routine clinicalanalyses to cutting-edge research reagents and custom conjugates to enhance yourresearch capabilities. BD Biosciences also offers a complete line of quality assuranceproducts and services for instrument validation, method control, reliable gating,absolute counting, and within-panel consistency checks.

Figure 1

Unique Sorting Technology

The BD FACSCalibur system features a unique catcher tube mechanism that moves in and out of the sample core stream 300 times per second to capture designated cells after they pass through the laser. The cells are then directed to collection tubes or the Cell Concentrator Module (optional)for further processing.

To Collection

Catcher TubeMoves In and

Out of Stream

Sample Injection

To Waste

Routine Applications:

Productivity and Simplicity When it Counts

Laboratory technologists will appre-ciate the BD FACSCalibur system for its user-friendly design, high samplethroughput, improved workflowmanagement through automation, and simple yet sophisticated softwareapplications that enhance routineanalyses.

Whether you are performingimmunophenotyping of lymphocytesubsets, leukemia or lymphoma studies, transplantation research,residual white blood cell enumeration,or a wide range of other applications,the BD FACSCalibur system provides thetools you need to obtain results quickly,easily, and accurately.

Immunophenotyping

Characterizing cells at different stages of development through the use of fluorescent-labeled monoclonalantibodies against surface markers isone of the most common applicationsof flow cytometry. The BD FACSCalibursystem opens the window to this worldof information by providing multicolorcapability in an easy-to-use system.Instrument setup is as easy as a push of a button and a click of the mouse.Efficient collection optics, coupled to the flow cell with an optical gel,permit the use of a low-powered, air-cooled argon laser and a red diode laser to ensure the highsensitivity required for analysis of low-density antigens.

BD Biosciences also provides softwareand reagent systems to maximize yourlaboratory’s productivity. BD MultiSET™

software, in combination with BD MultiTEST™ reagents and BD Multi-Check controls, provides acomplete system for automating dataacquisition and analysis.

Our timesaving lyse/no-washmethodology‡ using BD MultiTESTreagents reduces sample handling and provides more answers per tube, leaving you with more time toperform other valuable tests. By using BD Biosciences exclusive Expert Gate,BD MultiSET software ensures that thecell population of interest is analyzed,increasing the accuracy and reliabilityof your results.

Absolute Counts

BD TruCOUNT™ Tubes make single-platform absolute counting easy—nosyringes, additional pipetting steps, orneed to integrate data from anotheranalyzer. Designed for use with clinicalproducts such as BD MultiTEST and BD TriTEST™ lymphocyte subsetreagents, BD LeucoCOUNT™ rWBCenumeration kit, and BD ProCOUNT™

progenitor cell enumeration kit, BD TruCOUNT Tubes bring precision to your counts and improvedproductivity to your lab. For researchapplications, BD Liquid Counting beads†

are also available for quick estimationsof cell culture or bacterial concentration.


Figure 2

Clinical Lab Reports

The BD MultiSET software Lab Report containslymphocyte subset information, includingoptional absolute counts, reference-rangechecks, T-helper/suppressor ratio, and tube-to-tube consistency checks.


Residual White Blood CellEnumeration

The BD LeucoCOUNT system is the first automated, IVD-cleared product for monitoring leucoreduced bloodproducts. Together with BD TruCOUNTTubes and BD LeucoCOUNT processcontrols, the BD LeucoCOUNT systemprovides excellent accuracy, precision,and linearity. For high throughputwalkaway capability, the BD FACSLoader can also be added to your system.

DNA Analysis†

The DNA Analysis System componentswork together, from sample preparationto analysis, to eliminate many of thevariables associated with ploidy andcell-cycle analysis. The BD FACSCalibursystem’s optical and fluidic designensures that high-resolution DNA ploidy and S-phase determinations are easily achievable. Electronic doubletdiscrimination, utilizing the area andwidth of the fluorescence pulse, allowsfor the removal of artifacts—doubletsand aggregates—from the analysis.High-resolution electronics and linearityensure accurate results, and DNA QCparticles verify optimal performance.

Quality Assurance

BD Biosciences offers a complete familyof products designed to ensure thehighest quality clinical results from the BD FACSCalibur system.

BD FACSComp™ software, inconjunction with BD CaliBRITE™ beads,provides a comprehensive system to setup the instrument, assess sensitivity, andset compensation for immunofluorescenceapplications. For clinical applications, BD Biosciences offers a wide variety ofcellular controls to verify performancefor immunophenotyping, reticulocyteanalysis, stem cell enumeration, andrWBC applications.

104

103

102

101

100

SSC-

Hei

ght

100

FSC-Height

101 102 103 104

104

103

102

101

100

CD5

PE

100

CD7 FITC

101 102 103 104

104

103

102

101

100

CD3

PerC

P

100

CD45RO APC

101 102 103 104

104

103

102

101

100

CD3

PerC

P

100

CD2 PE

101 102 103 104

104

103

102

101

100

CD4

APC

100

CD8 FITC

101 102 103 104

red: aberrant (neoplastic) T cells

blue: normal T cells

green: normal NK cells

Figure 5

Peripheral T-cellLymphoma

Two four-color tubes are shown: 1) CD7 FITC vs CD5 PE and CD45ROAPC vs CD3 PerCP, and 2) CD2 PE vs CD3 PerCPand CD8 FITC vs CD4 APC.Aberrant T cells are col-ored red, normal T cellsare colored blue, andnormal NK cells are colored green.

1000

100

10

1

0.1

Mea

n O

bser

ved

Leuc

oCO

UN

T W

BC/µ

L

0.1

Adjusted Expected WBC/µL

1 10 100 1000

<5×106

<1×106

Decision PointArea

Figure 3

BD LeucoCOUNT Kit

The BD LeucoCOUNT system provides excellentaccuracy, precision, and linearity at clinicallysignificant levels of detection for RBC (red) and platelet (blue) samples.

Figure 4

DNA Analysis

Pulse processing is a powerful tool used in the discrimination of doublets for optimal DNA analysis.

Mean Observed LeucoCOUNT WBC/µL =2.607 + 0.871 × Expected WBC/µL; R2 = 0.955

Mean Observed LeucoCOUNT WBC/µL =2.420 + 0.829 × Expected WBC/µL; R2 = 0.931


Advanced Applications:

Performance and Flexibility When it Counts

Researchers and pathologists willappreciate the BD FACSCalibur systemfor its flexibility, which allows them toexpand their menu of applications astheir needs change. From genes toproteins to cells, this high-performancesystem is a valuable tool for assaydevelopment, verification, and physicalseparation of cells of interest for furthertesting or analysis.

Multicolor Analysis

Flow cytometry has rapidly evolvedtoward the use of multiple fluorochromesas an effective means of identifyingsubset populations within a singlesample—maximizing the informationgained from small samples, as well asincreasing the cost-effectiveness of eachassay. BD Biosciences pioneered thetechnology used in multicolor analysisand sets the industry standard with itsuse of two spatially separated laserbeams. This dual-laser technologyensures high sensitivity, minimalcompensation, and maximum flexibilityin fluorochrome selection. And the BD FACSCalibur system is the first toinclude diode laser technology andinterbeam compensation—all in analignment-free benchtop system.

Sorting†

Multiparameter flow cytometrycombined with cell sorting adds anadditional dimension to the informationyou gain from your sample. Purificationof subpopulations through sorting isuseful, eliminating contaminants that can interfere with sensitive assays,increasing the number of target cells in the sample, or confirming flowcytometric findings. Cell sorting is ideal for verifying cell morphology and performing subsequent moleculartechniques such as FISH or PCR, orfunctional assays. Cross-correlation ofthe flow cytometric information withresults of the post-sort assay is apowerful tool in cellular analysis.

www.bdbiosciences.com

Low-Angle, Light-Scatter Detector

Argon Laser

Beam-Shaping Optics

Red Diode Laser

High Performance Side-Scatter and

FluorescencePhotomultiplier

Lymphocyte Function Assessment†

The BD FastImmune™ assay system is an innovative flow cytometric activationassay using whole blood to directlyassess individual T-lymphocyte subset responses to a wide variety ofphysiological and nonphysiologicalstimuli. And unlike radioisotopemethods that are time and laborintensive, the BD FastImmune assay iscompleted in just hours. The system’sunique approach makes it the idealresearch tool for answering basicbiological questions regarding immuneregulation, T-cell subset responses to viral and bacterial antigens, and immune status assessment for chemotherapy, transplantation, and parasitology.


Figure 6A

Figure 6B

Figure 7

Bacterial Applications

Viability and concentration of bacteria from pharmaceutical, food, or environmental samplescan be demonstrated using propidium iodide (PI),thiazole orange‡ (TO), and BD Liquid countingbeads.† This sample of P. aeruginosa was prepared in staining buffer at 5 x 105 cells/uL.

Figure 8

Superantigen Activation of T Cells

The BD FastImmune Assay system†

can be employed to examine specificantigen responses in human wholeblood. Staphylococcal enterotoxin B (SEB) activates VB6 T-cell subsets but does not activate VB8 subsets, as determined by CD69 expression.‡

REST

92.75% 6.49%

95.28% 4.61%

100 101 102 103 104

100

101

102

103

104

100

101

102

103

104

100 101 102 103 104

CD69

PE

CD69

PE

Vβ6 FITC

Vβ8 FITC

SEB

15.45%

1.35%

78.82% 4.35%

18.90%

6.23%74.81%

100

101

102

103

104

100 101 102 103 104

100

101

102

103

104

100 101 102 103 104

CD69

PE

CD69

PE

Vβ6 FITC

Vβ8 FITC

Figures 6A&B

Sorting Results

PBMC and Daudi fractions were sorted for puritybased on immunofluorescence and scatter.Female PBMC and male Daudi were mixed andhybridized with chromosome Y (green) probesand counterstained with DAPI (red). Thehybridization of the sorted fractions show purefemale PMBC (Figure 6A, two red dots) andmale Daudi cells (Figure 6B, a red and a greendot), as expected. (Courtesy of Nga Bui, Tom Frey, PhD, SharylIwata, and Kathy Yokobata, BD Biosciences.)

R6Dead

R4

R5

Live

Injured

Additional Applications†

A thorough understanding of thebiology of the system under study,combined with the power and flexibilityof the BD FACSCalibur system, reagents,and data analysis tools, ensures thesuccessful application of flow cytometryin broad areas of biological andmedical research. Fluorescencequantitation with BD QuantiBRITE™

reagents, platelet analysis, stem cellresearch, multiparameter DNA analysis, BD Cytometric Bead Array, fluorescentprotein research, and microbial analysis are just a few of the forward-focusedapplication possibilities using the BD FACSCalibur system.

FACSCalibur 8/23/02 12:32 PM



Service and Support When it Counts

Because we understand how critical it is to have your system up and running, BD Biosciences backs its instruments, software, and reagents with BD FACService™,the most highly rated service and support program in the industry. BD FACServiceensures maximum instrument up-time and optimal performance with guaranteedresponse times, preventative maintenance programs, and highly trained service andapplication specialists.

In addition, we provide a variety of training packages and customized educational programs for your laboratory personnel that can be tailored to meet the requirements of users at any skill level. This ensures a shorter learningcurve and increased productivity for your laboratory.


The Gold Standard That Continues to Evolve

With its innovative reagents, powerful application software, and advancedcytometry hardware, the BD FACSCalibur system is the answer to your laboratoryneeds. Its built-in versatility meets the current demands of both clinical and research environments; its modularity takes you into the future. Best of all, the BD FACSCalibur system is backed by the leader in flow cytometry, BD Biosciences.

To find out more about how the BD FACSCalibur system can contribute to your lab’sproductivity and performance, contact your local BD Biosciences sales representative.

BD FACSCalibur System Specifications

Laser

488-nm, air-cooled Argon-ion laser

635-nm, air-cooled red diode laser (optional)

Fluorescence Sensitivity

Estimated detection limit is 200 FITC equivalent moleculesper particle.§

Fluorescence Resolution

Coefficient of variation of <3%, full peak, for propidiumiodide–stained chickenerythrocyte nuclei

Forward and Side ScatterSensitivity

Sensitivity enables theseparation of fixed plateletsfrom noise.

Forward and Side ScatterResolution

Scatter performance isoptimized for resolvinglymphocytes, monocytes, and granulocytes.

Sorting Specification

Sorting Purity: >95%

Sorting Rate: Up to 300 particles per second

Sort Modes: Single Cell,Exclusion, and Recovery

Power

US: 120 VAC +/- 10%

50/60 Hz +/-2 Hz

Current: 15 amps maximum

Outside US: Externaltransformer provided for 100 VAC +/-10%; 50/60 Hz and 220/240 VAC +/-10%; 50/60 Hz +/-2 Hz

Size

Sensor Module:

Width 91.4 cm (36 in)

Depth 61.5 cm (24.2 in)

Height 67.3 cm (26.5 in)

Cover Open: 124.5 cm (49 in)

Computer: 48 H x 41 D x 54 W cm(19 x 16 x 11 in)

Weight

Sensor Module: 109.1 kg (270 lbs)

Computer: 20 kg (44 lbs)

PRESORTEDSTANDARD

U.S. POSTAGEPAID

San Leandro, CAPermit No.169

Argentina/Paraguay/UruguayTel 54.11.4551.7100 x106Fax 54.11.4551.7400

Australia/New ZealandAustraliaTel 61.2.8875.7000Fax [email protected] ZealandTel 64.9.574.2468Fax [email protected]

AustriaScientific Support Tel 43.1.706.36.60.44Fax [email protected] ServiceTel 43.1.706.36.60Fax 43.1.706.36.60.11

Belgium Tel 32.53.720.600Fax [email protected] ServiceTel 32.53.720.550Fax [email protected]

BrazilTel 55.11.5185.9995Fax 55.11.5185.9895

Central America/CaribbeanTel 506.290.7318Fax 506.290.7331

ChileTel 56.2 460.0380 x21Fax 56.2 460.0306

ChinaTel 8610.6418.1608Fax 8610.6418.1610

ColombiaTel 57.1.345.0510Fax 57.1.255.6320

DenmarkTel 45.43.43.45.66Fax [email protected]

East AfricaTel 254.2.341157Fax [email protected]

Eastern EuropeTel 49.6221.305.161Fax 49.6221.305.418

EgyptTel 202.268.0181Fax 202.266.7562

FinlandTel 358.9.88.70.7832Fax [email protected]

FranceTel 33.4.76.68.36.36Fax 33.4.76.68.35.06Scientific Support Tel 33.4.76.68.34.25Fax 33.4.76.68.55.71Customer ServiceTel 33.4.76.68.37.32Fax 33.4.76.68.35.06

GermanyScientific Support Tel 49.6221.305.525Fax [email protected] ServiceTel 49.6221.305.551Fax [email protected]

GreeceTel 30.1.940.77.41Fax 30.1.940.77.40

Hong KongTel 852.2575.8668Fax 852.2803.5320

HungarySzerenaTel 36.1.345.7090Fax 36.1.345.7093

IndiaTel 91.124.638.3566.77/3219Fax 91.124.638.3225

IndonesiaTel 62.21.577.1920Fax 62.21.577.1925

ItalyTel 39.02.48.240.1Fax 39.02.48.20.33.36

JapanFujisawa Pharmaceutical Co., Ltd.(Reagents from ImmunocytometrySystems & Pharmingen)Tel 81.6.6206.7890Fax 81.6.6206.7934

KoreaTel 822.3404.3773Fax 822.557.4048

MalaysiaTel 603.7725.5517Fax 603.7725.4772

MexicoTel 52.55.5999.8296Fax 52.55.5999.8288

Middle EastTel 971.4.337.95.25Fax 971.4.337.95.51

Netherlands Tel 31.20.582.94.24Fax [email protected] ServiceTel 31.20.582.94.20Fax [email protected]

NorwayImmunocytometry Systems &PharmingenLaborel S/ATel 47.23.05.19.30Fax 47.22.63.07.51ClontechTel 46.8.775.5110Fax 46.8.775.5111

Peru/Bolivia/EcuadorTel 51.1.430.0323Fax 51.1.430.1077

PhilippinesTel 632.807.6073Fax 632.850.1998

PolandTel 48.22.651.53.00Fax 48.22.651.79.24

PortugalEnzifarmaTel 351.21.422.01.00Fax 351.21.422.01.10

Saudi ArabiaTel 966.1.26.00.805/806Fax 966.1.26.00.804

South AfricaTel 27.11.807.15 31Fax 27.11.807.19 53

SpainImmunocytometry Systems &PharmingenScientific Support Tel 34.91.848.81.77Fax 34.91.848.81.05Customer ServiceTel 34.902.27.17.27Fax 34.91.848.81.04ClontechTel 34.91.848.81.85Fax 34.91.848.81.04

SwedenTel 46.08.775.51.10Fax [email protected]

SwitzerlandScientific Support Tel 41.61.485.22.91Fax [email protected] ServiceTel 41.61.485.22.22Fax [email protected]

TaiwanTel 8862.2722.5660Fax 8862.2725.1768

ThailandTel 662.643.1371Fax 662.643.1381

TurkeyTel 90.212.222.87.77Fax 90.212.222.87.76

UKTel 44.1865.78.16.66Fax 44.1865.78.16.27Scientific Support Tel 44.1865.78.15.66

VenezuelaTel 58.212.443.6411 x248Fax 58.212.442.4477

West AfricaSobidisTel 225.20.33.40.32Fax 225.20.33.40.28

BD BiosciencesClontechDiscovery LabwareImmunocytometry SystemsPharmingen

6/2002 23-3090-03

BD Biosciences Pharmingen10975 Torreyana Rd.San Diego, CA 92121-1106Toll free 877.232.8995 Tel 858.812.8800 Fax 858.812.8888

Asia PacificBD SingaporeTel 65.6861.0633Fax 65.6860.1590

United StatesBD BiosciencesClontechFax 650.354.0775Discovery LabwareFax 978.901.7493Immunocytometry SystemsFax 408.954.2347PharmingenFax 858.812.8888Customer/Technical ServiceToll free 877.232.8995www.bdbiosciences.com

JapanNippon Becton DickinsonTel 81.24.593.5405Fax 81.24.593.5761

Clontech Company(Clontech Products)Tel 81.3.5324.9609Fax 81.3.5324.9637

EuropeBelgiumTel 32.53.720.211Fax [email protected]

CanadaBD BiosciencesToll free 888.259.0187Tel 905.542.8028Fax [email protected]

* For In Vitro Diagnostic use.

† For Research Use Only. Not for use in diagnostic or therapeutic procedures.

‡ Lyse/No-Wash Method: US Patent No. 5,776,709; FastImmune activation measurement: US Patent Nos.5,445,939; 5,656,446; 5,834, 5, 843,689,689; European Patent No. 319,543; Canadian Patent No. 1,296,622;Australian Patent No. 615,880; and Japanese Patent No. 2,769,156; Thiazole Orange: US Patent Nos. 4,883,867and 4,957,870

§ Data obtained from three representative instruments using Spherotech Rainbow RCP 30-5A particles.

The BD FACSCalibur instrument is a Class I laser product per IEC 825-1 and 21 CFR 1040.

BD, the BD logo, and all other trademarks are the property of Becton, Dickinson and Company

© 2002 BD

visit us at BDBiosciences.com

BD FACSCalibur Flow Cytometer - Florida Atlantic Universitymed.fau.edu/calibur.pdf · BD...

Documents

Transcript of BD FACSCalibur Flow Cytometer - Florida Atlantic Universitymed.fau.edu/calibur.pdf · BD...