Single Cell Capture

Single bacterial spore detection Single bacterial spore detection with magnetoelastic particle sensors with magnetoelastic particle sensors

on a microfluidic platformon a microfluidic platformAuburn University Detection and Auburn University Detection and

Food Safety CenterFood Safety CenterJ.W. HongJ.W. Hong

Chip Design – Top ViewChip Design – Top View

Unit: [um]Unit: [um]

Fluidic channels for sample loadingFluidic channels for sample loadingFluidic channels for sensor loading Fluidic channels for sensor loading Control channels Control channels

Top View (Enlarged)Top View (Enlarged)

Unit: [um]Unit: [um]

3D View3D View

3D View (Enlarged)3D View (Enlarged)

Multilayer Soft LithographyMultilayer Soft Lithography

Pneumatic Controlled ValvesPneumatic Controlled Valves

Valve ActuationValve Actuation

Valve actuation pressure: < 3.5 psiValve actuation pressure: < 3.5 psi

Chip Operation with Food DyesChip Operation with Food Dyes

Fluoresbrite YG 2.0-um microspheres (Polysciences, Inc.)Fluoresbrite YG 2.0-um microspheres (Polysciences, Inc.)

Chip Operation Chip Operation with Fluorescent Microsphereswith Fluorescent Microspheres

Sensor LoadingSensor Loading

A particle sensor in the chamberA particle sensor in the chamber

Spore CaptureSpore Capture

2um-fluorescent microspheres

Particle sensor

Single Cell Capture

Documents

Transcript of Single Cell Capture

Single Cell Proteins: “Future Fit” Ingredients...Single Cell Proteins: “Future-Fit” Feed Ingredients 21 •Single cell proteins come in many different forms •Shouldn’t

Single cell protein.

Single Cell Biosensor

capture-recapture Single Defect

Carbon Capture with Fuel Cell Power Plants Capture... · Carbon Capture with Fuel Cell Power Plants ... as a side reaction of power generation ... Capture Using Carbonate Fuel Cells

Realistic Single Cell Modeling – from Experiment to ...csdori/elte_NEURON/single_cell_modelling... · Realistic Single Cell Modeling – from ... Realistic Single Cell Modeling

g l e C e l Bi Single-Cell Biology · Single Cell Biol, an oen ae onal ole 7 Ie 3 1000173 ISS: 2168-9431 developed a laser capture microdissection (LCM)-proteomics analysis workflow.

Predicting Cell Capture from Dilute Samples for ... cell capture-Mosier.pdf · Predicting Cell Capture from Dilute Samples for Microfluidic Biosensors Nathan S. Mosier and Bruce Craig

Illumina®|Bio-Rad® Single Cell Sequencing Solution...Single Cell Isolation Principle Single-Cell Suspension Cell Isolation Reagents (including barcodes) Isolate thousands of cells

Single Cell Organisms

BD Rhapsody™ Single-Cell Analysis System Instrument ......Pipette programs BD Rhapsody P1200M and P5000M pipettes are provided pre-programmed for use during single cell mRNA capture

Dual and Single Channel Live Capture CardsThe VisionLC range of “Live Capture” cards provide single and dual channel high definition capture channels in a PCIe low profile card.

inDrop Single Cell Encapsulation and Reverse Transcription Protocol ... · Single Cell Encapsulation Protocol, Version 2.1 inDropTM Single Cell Encapsulation and Reverse Transcription

Single Cell Isolation

Single Cell Variability

SINGLE-CELL BIOINFORMATICS & MACHINE LEARNING · Goal: develop new bioinformatics and machine-learning approaches to study single-cell gene regulatory networks Tools: single-cell

BD Single-Cell Multiplexing Kit · BD ™ Single-Cell Multiplexing Kit Reduce single-cell sequencing reagent cost and improve data quality in single-cell sequencing analysis *Does

Cell Communication Single cell Multicellular organism.

Single-cell methylomes identify neuronal subtypes and ...papers.cnl.salk.edu/PDFs/Single-cell methylomes... · NEURAL EPIGENOMICS Single-cell methylomes identify neuronal subtypes

Single Cell Genomics...Cell capture/RT/library prep (B and T cell enrichment) $2,460/Sample 10x Genomics Chromium 500-10,000 cells/sample Sequencing for 10x Genomics 3’ single-cell