Single Cell Isolation
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Transcript of Single Cell Isolation
Sample to Insight
Single Cell Isolation
Find out how you can isolate the single cell of your choice and get visual confirmation
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QIAscout: single cell isolation
High-density microraft array-based single cell isolation – simple, easy, affordable. You only require an inverted microscope to start working with the device.
Single cell genomics by QIAGEN, 2016
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Components of the QIAscout system
Understand the elegant simplicity of the QIAscout on YouTube
Single cell genomics by QIAGEN, 2016
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Discover QIAscout: single cell isolation
• Access to single cells for every lab
• Number of single cells easily scalable
• Imaging-based cell selection
• Easy to use◦ Choose the cell, release microraft to downstream reaction tube◦ Repeat as often as you want
• Affordable◦ Cost-effective device usable on multiple inverted microscopes present in labs
• Cells stay viable◦ Array can be used as a standard cell culture dish◦ Enables single cell clonal expansion
• Compatible with downstream NGS, WGA, WTA and PCR
Single cell genomics by QIAGEN, 2016
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QIAscout: an easy start to your single cell analysis workflow
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Ideally suited for• Single cell isolation from cell suspensions
• Low volume, precious samples
• Adherent or non-adherent eukaryotic cells from various sample types
• Downstream molecular analyses◦ Aligned with QIAGEN’s single cell analysis portfolio
• Clonal expansion
“QIAscout turned out to be a very suitable system, allowing us to select and transfer our cell of interest with nearly 100% efficiency and survival rate for further downstream applications. The instrument has contributed greatly to our research. ”
Dr. Lajos Kemeny, University of Szeged, Hungary
Single cell genomics by QIAGEN, 2016
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The QIAscout array
• Can be used as standard cell culture dish• Microrafts are magnetic, dislodgeable
and self-sealing• Each array contains 12,000 microrafts
• Cells settle according to Poisson distribution
• 6000 cells equals a 30% chance of having a singe cell in a microraft
Single cell genomics by QIAGEN, 2016
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CellTracker Green
Hoechst
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Image of cells on the QIAscout array
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After isolation cells remain viable and continue to grow
Pierced cluster of HT-29 cells
The same cluster, after several days
of cultivation
Pierced SW48 cell After severll days of cultivation
5 h 24 h 45 h 90 h
MCF-7 cell culture, established after transfer of pierced cell
HT-29 cells after 90 hours
Single cell genomics by QIAGEN, 2016
Read the application note
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Single cell genomics by QIAGEN
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For in-depth, molecular analysis of single cells
Single cell WGA or WTA
Single cell isolation
Single cell sequencing
• Easily access single cells
• Affordable
• Gentle on cells
QIAscoutQIAseq
FX Single Cell Library Kits
REPLI-g Single Cell Kits
Single cell miRNA analysis
• Create superior-quality NGS libraries directly from single cells
• Provides best-in-class amplification of genomes or transcriptomes from single cells
• Profile miRNA expression using qPCR
miScript Single Cell qPCR Kit
Single cell genomics by QIAGEN, 2016
Visit the single cell resource center