Single Cell Capture
12
Single bacterial spore Single bacterial spore detection detection with magnetoelastic particle with magnetoelastic particle sensors sensors on a microfluidic platform on a microfluidic platform Auburn University Auburn University Detection and Food Detection and Food Safety Center Safety Center J.W. Hong J.W. Hong
-
Upload
alison-zunklei -
Category
Documents
-
view
225 -
download
3
description
Capture of a single cell on a microfluidic chip.
Transcript of Single Cell Capture
Single bacterial spore detection Single bacterial spore detection with magnetoelastic particle sensors with magnetoelastic particle sensors
on a microfluidic platformon a microfluidic platformAuburn University Detection and Auburn University Detection and
Food Safety CenterFood Safety CenterJ.W. HongJ.W. Hong
Chip Design – Top ViewChip Design – Top View
Unit: [um]Unit: [um]
Fluidic channels for sample loadingFluidic channels for sample loadingFluidic channels for sensor loading Fluidic channels for sensor loading Control channels Control channels
Top View (Enlarged)Top View (Enlarged)
Unit: [um]Unit: [um]
3D View3D View
Fluidic channels for sample loadingFluidic channels for sample loadingFluidic channels for sensor loading Fluidic channels for sensor loading Control channels Control channels
3D View (Enlarged)3D View (Enlarged)
Multilayer Soft LithographyMultilayer Soft Lithography
Pneumatic Controlled ValvesPneumatic Controlled Valves
Valve ActuationValve Actuation
Valve actuation pressure: < 3.5 psiValve actuation pressure: < 3.5 psi
Fluidic channels for sample loadingFluidic channels for sample loadingFluidic channels for sensor loading Fluidic channels for sensor loading Control channels Control channels
Chip Operation with Food DyesChip Operation with Food Dyes
Fluoresbrite YG 2.0-um microspheres (Polysciences, Inc.)Fluoresbrite YG 2.0-um microspheres (Polysciences, Inc.)
Chip Operation Chip Operation with Fluorescent Microsphereswith Fluorescent Microspheres
Sensor LoadingSensor Loading
A particle sensor in the chamberA particle sensor in the chamber
Spore CaptureSpore Capture
2um-fluorescent microspheres
Particle sensor
