Groups members

Ayesha Aftab Malik Amber Malik

What is a Protein?

Proteins are the workhorses that facilitate most biological processes in a cell.

Critical aspects required to understand the function of a protein

Expression profile

Post-translational modifications

Intracellular localization

Interactions with other proteins

Evolutionary history and conserved sequence

Protein sequence and structure

PROTEIN PROTEIN INTERACTION

Need of protein protein interaction

The majority of proteins interact with other proteins for proper function

Publication of the human genome Development of the field of

proteomics,

TYPES OF PROTEIN PROTEIN INTERACTION

Two types Stable Transient Both types can be weak or strong

Biological effect of protein protein interaction

Alter the kinetic properties of enzymes Allow for substrate channeling Create a new binding site Inactivate or destroy a protein Change the specificity of a protein for

interaction with different proteins Serve a regulatory role

Common Methods to Analyze Protein-Protein Interactions In Wet Lab

Co-ImmunoprecipetationPull-down assay

Cross linking protein interaction analysisLabel transfer protein interaction analysis

Far-western blot analysis

DEFECTS IN WET LAB PROCESSES Slow Time consumingNot accurateDifficult to handle CostlyLack of equipments

Common methods of PIP’s in BioinformaticsPrimary databasesInt actMintBindstring

Meta databasesPINAAPIP

DATABASES

STRING:  known and predicted protein interactions.

Interactions physical and functional associations

The database currently covers 5'214'234 proteins from 1133 organisms.

DATABASES

K VOTE to create seven different integrated

networks by using values of k ranging from 1-7.

modularity, similarity-based modularity, clustering score, enrichment.

Detection and quantification of protein interactions

Databases of protein-protein interactions and their use in drug discovery.

Proteins rarely act alone

Case of use of PPI’s

Questions