Protein–protein interaction networks

Lars Juhl Jensen

Phase 1association networks

who?

STRING

Jensen, Kuhn et al., Nucleic Acids Research, 2009

STITCH

Kuhn et al., Nucleic Acids Research, 2010

630 genomes

2.5 million proteins

~74,000 small molecules

genomic context

Korbel et al., Nature Biotechnology, 2004

Korbel et al., Nature Biotechnology, 2004

Korbel et al., Nature Biotechnology, 2004

gene coexpression

physical interactions

Jensen & Bork, Science, 2008

genetic interactions

Beyer et al., Nature Reviews Genetics, 2007

curated knowledge

Letunic & Bork, Trends in Biochemical Sciences, 2008

literature mining

>10 km

confidence scores

benchmarking

von Mering et al., Nucleic Acids Research, 2005

transfer by orthology

protein mode

von Mering et al., Nucleic Acids Research, 2005

COG mode

von Mering et al., Nucleic Acids Research, 2005

probabilistic integration

Frishman et al., Modern Genome Annotation, 2009

Phase 2signaling networks

what?

phosphoproteomics

signaling events

sequence motifs

Miller, Jensen et al., Science Signaling, 2008

NetPhorest

Miller, Jensen et al., Science Signaling, 2008

NetworKIN

Linding, Jensen, Ostheimer et al., Cell, 2007

benchmarking

Linding, Jensen, Ostheimer et al., Cell, 2007

context is crucial

use cases

DNA damage response

Linding, Jensen, Ostheimer et al., Cell, 2007

cell-cycle kinases

Olsen, Vermeulen et al., Science Signaling, 2010

Phase 3dynamic networks

when?

cell-cycle regulation

microarray time courses

Gauthier et al., Nucleic Acids Research, 2007

protein interactions

temporal network

de Lichtenberg, Jensen et al., Science, 2005

de Lichtenberg, Jensen et al., Science, 2005

just-in-time assembly

de Lichtenberg, Jensen et al., Cell Cycle, 2007

evolutionary flexibility

individual genes

Jensen, Jensen, de Lichtenberg et al., Nature, 2006

protein complexes

Jensen, Jensen, de Lichtenberg et al., Nature, 2006

phosphorylation

co-evolution

Jensen, Jensen, de Lichtenberg et al., Nature, 2006

summary

networks

data integration

specific predictions

systems properties

AcknowledgmentsNetPhorest.info

– Rune Linding– Martin Lee Miller– Francesca Diella– Claus Jørgensen– Michele Tinti– Lei Li– Marilyn Hsiung– Sirlester A. Parker– Jennifer Bordeaux– Thomas Sicheritz-Pontén– Marina Olhovsky– Adrian Pasculescu– Jes Alexander– Stefan Knapp– Nikolaj Blom– Peer Bork– Shawn Li– Gianni Cesareni– Tony Pawson– Benjamin E. Turk– Michael B. Yaffe– Søren Brunak

STRING-DB.org– Christian von Mering– Michael Kuhn– Manuel Stark– Samuel Chaffron– Chris Creevey– Jean Muller– Tobias Doerks– Philippe Julien– Alexander Roth– Milan Simonovic– Jan Korbel– Berend Snel– Martijn Huynen– Peer Bork

Cyclebase.org– Ulrik de Lichtenberg– Thomas Skøt Jensen– Nicholas Paul Gauthier– Rasmus Wernersson– Søren Brunak

NetworKIN.info– Rune Linding– Gerard Ostheimer– Heiko Horn– Martin Lee Miller– Francesca Diella– Karen Colwill– Jing Jin– Pavel Metalnikov– Vivian Nguyen– Adrian Pasculescu– Jin Gyoon Park– Leona D. Samson– Rob Russell– Peer Bork– Michael Yaffe– Tony Pawson

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