Protein protein interaction basic

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this presentation include info for stater students and have basic knowledge of pro pro interaction

Transcript of Protein protein interaction basic

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Groups members

Ayesha Aftab Malik Amber Malik

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What is a Protein?

Proteins are the workhorses that facilitate most biological processes in a cell.

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Critical aspects required to understand the function of a protein

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Expression profile

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Post-translational modifications

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Intracellular localization

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Interactions with other proteins

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Evolutionary history and conserved sequence

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Protein sequence and structure

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PROTEIN PROTEIN INTERACTION

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Need of protein protein interaction

The majority of proteins interact with other proteins for proper function

Publication of the human genome Development of the field of

proteomics,

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TYPES OF PROTEIN PROTEIN INTERACTION

Two types Stable Transient Both types can be weak or strong

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Biological effect of protein protein interaction

Alter the kinetic properties of enzymes Allow for substrate channeling Create a new binding site Inactivate or destroy a protein Change the specificity of a protein for

interaction with different proteins Serve a regulatory role

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Common Methods to Analyze Protein-Protein Interactions In Wet Lab

Co-ImmunoprecipetationPull-down assay

Cross linking protein interaction analysisLabel transfer protein interaction analysis

Far-western blot analysis

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DEFECTS IN WET LAB PROCESSES Slow Time consumingNot accurateDifficult to handle CostlyLack of equipments

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Common methods of PIP’s in BioinformaticsPrimary databasesInt actMintBindstring

Meta databasesPINAAPIP

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DATABASES

STRING:  known and predicted protein interactions.

Interactions physical and functional associations

The database currently covers 5'214'234 proteins from 1133 organisms.

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DATABASES

K VOTE to create seven different integrated

networks by using values of k ranging from 1-7.

modularity, similarity-based modularity, clustering score, enrichment.

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Detection and quantification of protein interactions

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Databases of protein-protein interactions and their use in drug discovery.

Proteins rarely act alone

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Case of use of PPI’s

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Questions

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