The Nebraska Center for Rapid The Nebraska Center for Rapid BioanalysisBioanalysis

An Overview of the NIH COBRE ProgramAn Overview of the NIH COBRE Program

David S. Hage

COBRE Retreat - January 16, 2009

Overview of PresentationOverview of Presentation An Introduction to the NIH COBRE An Introduction to the NIH COBRE

ProgramProgram

Rationale for a COBRE Proposal in Rapid Rationale for a COBRE Proposal in Rapid Bioanalysis Bioanalysis

Details behind the COBRE Proposal to Details behind the COBRE Proposal to create a Center for Rapid Bioanalysiscreate a Center for Rapid Bioanalysis

What is the NIH COBRE Program?What is the NIH COBRE Program?((CCenters enters oof f BBiomedical iomedical RResearch esearch EExcellence)xcellence)

Program Objectives:Program Objectives: To strengthen an institution’s biomedical To strengthen an institution’s biomedical

research infrastructure through the research infrastructure through the establishment of a thematic multi-establishment of a thematic multi-disciplinary centerdisciplinary center

To enhance the ability of investigator’s to To enhance the ability of investigator’s to compete independently for complementary compete independently for complementary NIH individual research grantsNIH individual research grants

Details of the NIH COBRE ProgramDetails of the NIH COBRE Program Program supports initiation and development of unique, state-of-the-art Program supports initiation and development of unique, state-of-the-art

biomedical and behavior research in the full spectrum of basic and biomedical and behavior research in the full spectrum of basic and clinical sciencesclinical sciences

Application must have a thematic focusApplication must have a thematic focus

COBRE awards are supported through the IDeA Program, aimed at COBRE awards are supported through the IDeA Program, aimed at states with historically low aggregate success rates for grants from NIHstates with historically low aggregate success rates for grants from NIH

A substantial commitment on the part of the institution is required to A substantial commitment on the part of the institution is required to provide additional faculty lines in the thematic areaprovide additional faculty lines in the thematic area

Awards are made for 5 years, renewable, with up to 1.5 million (direct Awards are made for 5 years, renewable, with up to 1.5 million (direct costs) being awarded per year; an additional 500 K may be requested costs) being awarded per year; an additional 500 K may be requested in year 1 for facility renovationsin year 1 for facility renovations

Structure of a COBRE GrantStructure of a COBRE Grant Description of PIDescription of PI

Qualifications – Background, Funding History, Mentoring, etc.Qualifications – Background, Funding History, Mentoring, etc.

Overall ResearchOverall Research• Descriptions of 3-5 projects to be performed by junior facultyDescriptions of 3-5 projects to be performed by junior faculty

Administrative CoreAdministrative Core• Organization, plans for development of Center and junior facultyOrganization, plans for development of Center and junior faculty

Research Core FacilitiesResearch Core Facilities

Alteration & Renovation (if applicable)Alteration & Renovation (if applicable)

Rationale for Creation of Nebraska Center Rationale for Creation of Nebraska Center for Rapid Bioanalysisfor Rapid Bioanalysis

Many areas of research in biotechnology, medical Many areas of research in biotechnology, medical research and pharmaceutical science are turning research and pharmaceutical science are turning to more rapid screening or analysis methodsto more rapid screening or analysis methods

Examples include:Examples include:Proteomics Proteomics Glycomics GlycomicsMetabolomicsMetabolomics DNA Sequencing DNA SequencingHigh-throughput Screening of DrugsHigh-throughput Screening of DrugsIdentification & Analysis of BiomarkersIdentification & Analysis of BiomarkersClinical AssaysClinical AssaysCombinatorial ChemistryCombinatorial Chemistry

Rationale for Creation of Nebraska Center Rationale for Creation of Nebraska Center for Rapid Bioanalysis (Cont’d)for Rapid Bioanalysis (Cont’d)

Need for Need for improved and more rapid bioanalytical improved and more rapid bioanalytical methods is recognized in several parts of NIH methods is recognized in several parts of NIH Roadmap for Medical ResearchRoadmap for Medical Research Building Blocks, Biological Pathways, and NetworksBuilding Blocks, Biological Pathways, and Networks

Molecular Libraries and ImagingMolecular Libraries and Imaging

Human Microbiome Project Human Microbiome Project

Among the more than 70 COBRE programs in Among the more than 70 COBRE programs in existence, none deals primarily with bioanalysisexistence, none deals primarily with bioanalysis

Current Research Interests/Strengths at Current Research Interests/Strengths at UNL & UNMC in Rapid BioanalysisUNL & UNMC in Rapid Bioanalysis

Mass SpectrometryMass Spectrometry

NMR NMR

Chemical Separations Chemical Separations

Electrochemistry Electrochemistry

Optical Spectroscopy Optical Spectroscopy

o Although instrumentation and facilities are available in these Although instrumentation and facilities are available in these areas, UNL and UNMC currently lack a critical mass of areas, UNL and UNMC currently lack a critical mass of researchers and the infrastructure needed to achieve national researchers and the infrastructure needed to achieve national leadership in bioanalysisleadership in bioanalysis

Rapid PCR/DNA Sequencing Rapid PCR/DNA Sequencing Microfluidics/NanofabricationMicrofluidics/NanofabricationCombinatorial ChemistryCombinatorial ChemistrySingle Molecule Imaging & Single Molecule Imaging &

ManipulationManipulation

Complementary Nature of Center for Rapid Complementary Nature of Center for Rapid Bioanalysis with Other COBRE Bioanalysis with Other COBRE Programs/Efforts in NebraskaPrograms/Efforts in Nebraska

Center for Virology (UNL)Center for Virology (UNL)

Center for Redox Biology (UNL)Center for Redox Biology (UNL)

Cell signaling (UNMC)Cell signaling (UNMC)

Neurosensory systems (UNMC)Neurosensory systems (UNMC)

Nanomedicine (UNMC)Nanomedicine (UNMC)

Fit of Proposed Center with Other Research Fit of Proposed Center with Other Research at UNL & UNMCat UNL & UNMC

Proposed Center would be complementary to current Proposed Center would be complementary to current research in the areas of pharmaceutical science, research in the areas of pharmaceutical science, combinatorial chemistry, chemical engineering, combinatorial chemistry, chemical engineering, biochemistry, molecular biology, and environmental biochemistry, molecular biology, and environmental toxicologytoxicology

A similar fit can be made with efforts in biotechnology, A similar fit can be made with efforts in biotechnology, food science, bioinformatics, & general medical food science, bioinformatics, & general medical research or cancer researchresearch or cancer research

Program would also have a good fit with several local Program would also have a good fit with several local industries (LICOR, MDS Pharma, etc.)industries (LICOR, MDS Pharma, etc.)

History of Current COBRE Proposal from UNLHistory of Current COBRE Proposal from UNL

Up to three COBRE Awards allowed per institution (e.g., UNL has Up to three COBRE Awards allowed per institution (e.g., UNL has two – Virology & Redox Biology)two – Virology & Redox Biology)

Only one full proposal can be submitted per institutionOnly one full proposal can be submitted per institution

Current proposal was selected by UNL for development in 2007 Current proposal was selected by UNL for development in 2007 and was submitted Oct. 24, 2007and was submitted Oct. 24, 2007

Reviews of current proposal were received in June 2008, with an Reviews of current proposal were received in June 2008, with an Overall rating of “Excellent” . Final announcement of funding will Overall rating of “Excellent” . Final announcement of funding will be made in Spring 2009. be made in Spring 2009.

If not funded this round, a revision of this proposal is planned for If not funded this round, a revision of this proposal is planned for the next call for COBRE proposals, which is expected to be made the next call for COBRE proposals, which is expected to be made in Jan/Feb 2009in Jan/Feb 2009

Specific Proposal Submitted by UNLSpecific Proposal Submitted by UNL::Nebraska Center for Rapid Bioanalysis (NCRB)Nebraska Center for Rapid Bioanalysis (NCRB)

Overall Objectives Overall Objectives Creation of a Center at UNL and in cooperation with UNMC focusing on Creation of a Center at UNL and in cooperation with UNMC focusing on

the development of methods for the rapid analysis and high-throughput the development of methods for the rapid analysis and high-throughput screening of biological agentsscreening of biological agents

Support and mentoring of junior faculty in the field of rapid bioanalysis Support and mentoring of junior faculty in the field of rapid bioanalysis and related areas and related areas

Development and expansion of the infrastructure at UNL, UNMC and in Development and expansion of the infrastructure at UNL, UNMC and in the state of Nebraska to support research in the field of rapid the state of Nebraska to support research in the field of rapid bioanalysisbioanalysis

Center would be housed in Dept. Chemistry at UNL but directly involve Center would be housed in Dept. Chemistry at UNL but directly involve faculty from the Depts. of Chemical & Biomolecular Engineering and faculty from the Depts. of Chemical & Biomolecular Engineering and Biological Systems Engineering at UNL, as well as faculty in Depts. of Biological Systems Engineering at UNL, as well as faculty in Depts. of Pharmaceutical Science and Biochemistry & Molecular Biology at Pharmaceutical Science and Biochemistry & Molecular Biology at UNMC (among many others) UNMC (among many others)

Specific Aims of the Specific Aims of the NCRBNCRB

Specific Aim 1Specific Aim 1: : Mobilize and expand the collective Mobilize and expand the collective expertise at UNL and UNMC in the areas of chemistry, expertise at UNL and UNMC in the areas of chemistry, biochemistry, engineering, pharmaceutical science and biochemistry, engineering, pharmaceutical science and basic medical research to develop, optimize, validate basic medical research to develop, optimize, validate and apply new technologies for rapid bioanalysis and apply new technologies for rapid bioanalysis

Specific Aim 2Specific Aim 2: : Establish a critical mass of Establish a critical mass of experienced researchers in the area of rapid bioanalysis experienced researchers in the area of rapid bioanalysis through the mentoring and training of junior through the mentoring and training of junior investigators, and through the targeted recruitment of investigators, and through the targeted recruitment of additional faculty members in this areaadditional faculty members in this area

Specific Aims of the Specific Aims of the NCRB NCRB (Cont’d)(Cont’d)

Specific Aim 3Specific Aim 3: : Develop/maintain an infrastructure Develop/maintain an infrastructure of support and facilities in areas such as bioinformatics of support and facilities in areas such as bioinformatics and computational chemistry, instrument development and computational chemistry, instrument development and microfabrication, structural and functional analysis, and microfabrication, structural and functional analysis, protein and cell production, and nanoimaging to enhance protein and cell production, and nanoimaging to enhance the research capability for new and existing investigators the research capability for new and existing investigators in the area of rapid bioanalysisin the area of rapid bioanalysis

Primary Elements for Meeting the Specific Aims of the NCRB

Administrative Core of the NCRBAdministrative Core of the NCRB

PI/Program Director (D.S. Hage, UNL)PI/Program Director (D.S. Hage, UNL)

Assistant Director (Y. Lyubchenko, UNMC)Assistant Director (Y. Lyubchenko, UNMC)

Junior Project LeadersJunior Project Leaders

Mentors & Mentoring CouncilMentors & Mentoring Council

External Advisory Board (EAB)External Advisory Board (EAB)

Industrial & Clinical Advisory Board (ICAB)Industrial & Clinical Advisory Board (ICAB)

Core FacilitiesCore Facilities

Overall Structure of the Nebraska Center for Rapid Overall Structure of the Nebraska Center for Rapid Bioanalysis (NCRB)Bioanalysis (NCRB)

Initial Research Focus of NCRB:Initial Research Focus of NCRB:Bioanalytical Tools for Drug Discovery & Biomarker DetectionBioanalytical Tools for Drug Discovery & Biomarker Detection

Drug DiscoveryDrug Discovery Use of proteomics, glycomics or metabolomics for drug discoveryUse of proteomics, glycomics or metabolomics for drug discovery Development of bioanalytical methods for rapidly identifying and characterizing new Development of bioanalytical methods for rapidly identifying and characterizing new

drugs to target a given protein or biological pathway drugs to target a given protein or biological pathway Creation of Creation of high-throughput screening (HTS)high-throughput screening (HTS) methods for examining potential drugs methods for examining potential drugs

and for characterizing their properties and for characterizing their properties

Biomarker DetectionBiomarker Detection Creation of improved methods for biomarker detection/identificationCreation of improved methods for biomarker detection/identification Improved analysis of biomarkers for disease treatment and detectionImproved analysis of biomarkers for disease treatment and detection Creation of improved methods for the study of biological pathways for biomarker Creation of improved methods for the study of biological pathways for biomarker

identificationidentification

Related TopicsRelated Topics Development of miniaturized or nanofabricated analytical systems for drug discovery Development of miniaturized or nanofabricated analytical systems for drug discovery

& biomarker detection& biomarker detection

Projects by Junior Faculty Projects by Junior Faculty

Project 1Project 1:: High-Throughput Screening of Drug Candidates by NMRHigh-Throughput Screening of Drug Candidates by NMR (Dr. (Dr. Robert Powers – Dept. Chemistry, UNL)Robert Powers – Dept. Chemistry, UNL)

Project 2Project 2:: Single Molecule Tracking & Imaging for High-Throughput Single Molecule Tracking & Imaging for High-Throughput ScreeningScreening (Dr. Greg Bashford – Dept. Biological Systems Engineering, UNL)(Dr. Greg Bashford – Dept. Biological Systems Engineering, UNL)

Project 3Project 3:: Cell Array Chips Based on Patterned NanostructuresCell Array Chips Based on Patterned Nanostructures (Dr. Barry (Dr. Barry Cheung – Dept. Chemistry, UNL)Cheung – Dept. Chemistry, UNL)

Project 4Project 4: : Folding-Based Electrochemical BiosensorsFolding-Based Electrochemical Biosensors (Dr. Rebecca Lai – (Dr. Rebecca Lai – Dept. Chemistry, UNL)Dept. Chemistry, UNL)

Project 5Project 5: : Proteomic & Metabolomic Studies of a Microbial CommunityProteomic & Metabolomic Studies of a Microbial Community

(Dr. Laurey Steinke – Dept. Biochem. & Mol. Biology, UNMC)(Dr. Laurey Steinke – Dept. Biochem. & Mol. Biology, UNMC)

General relationship between Junior Projects & Theme General relationship between Junior Projects & Theme of High-Throughput Screening of Drugs and Biomarker of High-Throughput Screening of Drugs and Biomarker

DetectionDetection

Mentoring of Junior Faculty: Mentoring of Junior Faculty: The Mentoring CouncilThe Mentoring Council

Mentoring of junior project leaders is an important part of Mentoring of junior project leaders is an important part of the COBRE programthe COBRE program

Each Junior Project Leader is assigned three mentorsEach Junior Project Leader is assigned three mentors A lead mentor in an area closely related to the project A lead mentor in an area closely related to the project A second mentor in an area complementary to the project A second mentor in an area complementary to the project Center Director or Assistant DirectorCenter Director or Assistant Director

Rationale behind mentor selectionRationale behind mentor selection One mentor for the method used in the project + one mentor for the area One mentor for the method used in the project + one mentor for the area

of biomedical applicationsof biomedical applications At least one mentor from UNL and at least one from UNMCAt least one mentor from UNL and at least one from UNMC Center Director or Assistant Director will be assigned based on the location Center Director or Assistant Director will be assigned based on the location

of the project and/or the area of research of the project and/or the area of research

Mentoring Council: Initial Members from UNLMentoring Council: Initial Members from UNL• Dr. Ronald CernyDr. Ronald Cerny - - mass spectrometry and applications in proteomics and the study of small molecules (L. Steinke)mass spectrometry and applications in proteomics and the study of small molecules (L. Steinke)

• Dr. Liangcheng DuDr. Liangcheng Du - - use of mol. biology and mass spectrometry to investigate pathways in microorganisms and use of mol. biology and mass spectrometry to investigate pathways in microorganisms and create new products (L. Steinke)create new products (L. Steinke)

• Dr. Gerard HarbisonDr. Gerard Harbison - - NMR spectroscopy (R. Powers)NMR spectroscopy (R. Powers)

• Dr. Natale (Ned) J. IannoDr. Natale (Ned) J. Ianno - Materials research & thin film deposition; pulsed laser deposition and sputter - Materials research & thin film deposition; pulsed laser deposition and sputter deposition of various materials (B. Cheung)deposition of various materials (B. Cheung)

• Dr. Larry Parkhurst Dr. Larry Parkhurst - A- Application of fluorescence spectroscopy in the study of biomolecular structure and function pplication of fluorescence spectroscopy in the study of biomolecular structure and function (G. Bashford)(G. Bashford)

• Dr. Jody RedepenningDr. Jody Redepenning - Electrochemistry and in the microfabrication of novel devices such as chemical-modified - Electrochemistry and in the microfabrication of novel devices such as chemical-modified nanoelectrodes (R. Lai)nanoelectrodes (R. Lai)

• Dr. David S. Hage Dr. David S. Hage – Bioanalytical separations, affinity-based methods, miniaturized analytical systems (R. Lai, R. – Bioanalytical separations, affinity-based methods, miniaturized analytical systems (R. Lai, R. Powers, L. Steinke)Powers, L. Steinke)

Mentoring Council: Initial UNMC MembersMentoring Council: Initial UNMC Members• Dr. Kenneth W. BaylesDr. Kenneth W. Bayles – M – Microbiologist; cellular biology of icrobiologist; cellular biology of S. aureus S. aureus andand Bacillus Bacillus

anthracis anthracis as related to biofilm formation & the development of antibiotics. (R. Powers)as related to biofilm formation & the development of antibiotics. (R. Powers)

• Dr. Steven H. HinrichsDr. Steven H. Hinrichs – Microbiologist; – Microbiologist; interests in new antibiotics, molecular interests in new antibiotics, molecular microbiology, and use of methods such as mass spectrometry (L. Steinke)microbiology, and use of methods such as mass spectrometry (L. Steinke)

• Dr. Michael HollingsworthDr. Michael Hollingsworth - M - Microbiologist and immunologist; pancreatic cancer icrobiologist and immunologist; pancreatic cancer and biomarkers for its detection (R. Lai)and biomarkers for its detection (R. Lai)

• Dr. Ming-Fong LinDr. Ming-Fong Lin - Biochemist and pathologist; interests in prostate cancer, cell - Biochemist and pathologist; interests in prostate cancer, cell growth regulation by tyrosine phosphorylation signal transduction and androgen growth regulation by tyrosine phosphorylation signal transduction and androgen regulation of cell proliferation (B. Cheung)regulation of cell proliferation (B. Cheung)

• Dr. Yuri Lybuchenko Dr. Yuri Lybuchenko – Pharmaceutical science, single molecule imaging, – Pharmaceutical science, single molecule imaging, fluorescence spectroscopy (G. Bashford, B. Cheung)fluorescence spectroscopy (G. Bashford, B. Cheung)

Evaluation & Advising of the NCRB: Evaluation & Advising of the NCRB: The External Advisory Committee (EAC)The External Advisory Committee (EAC)

The EAC will consist of outstanding scientists The EAC will consist of outstanding scientists representing the NCRB’s key research areas will provide representing the NCRB’s key research areas will provide an independent mechanism for evaluation of the an independent mechanism for evaluation of the research projects, core facilities and activitiesresearch projects, core facilities and activities

The EAC will also give advice on strategic planning, The EAC will also give advice on strategic planning, including allocation of resources and the targeting of including allocation of resources and the targeting of emerging funding opportunitiesemerging funding opportunities

The EAC meet annually on site with the Director and The EAC meet annually on site with the Director and Mentoring Council and provide an annual written critique Mentoring Council and provide an annual written critique of the Center as part of the formative evaluation processof the Center as part of the formative evaluation process

External Advisory Council (EAC)External Advisory Council (EAC)• Dr. Susan Lunte (Univ. Kansas) Dr. Susan Lunte (Univ. Kansas) – Creation of new tools for bioanalysis based on – Creation of new tools for bioanalysis based on

electrochemistry, chemical separations, electrochemical sensors, and microfabricated deviceselectrochemistry, chemical separations, electrochemical sensors, and microfabricated devices

• Dr. Fred W. McLafferty (Cornell Univ.)Dr. Fred W. McLafferty (Cornell Univ.) – Mass spectrometry & its applications in – Mass spectrometry & its applications in various systemsvarious systems

• Dr. John L. Markley (Univ. Wisconsin) Dr. John L. Markley (Univ. Wisconsin) - Use of NMR spectroscopy in the study of - Use of NMR spectroscopy in the study of structural genomics, metabolomics and structure-function relationships in proteins; multi-structural genomics, metabolomics and structure-function relationships in proteins; multi-dimensional NMRdimensional NMR

• Dr. Irving Wainer (NIH)Dr. Irving Wainer (NIH) - Bioanalytical chemistry, use of mass spectrometry and - Bioanalytical chemistry, use of mass spectrometry and chromatography in the study of biological sampleschromatography in the study of biological samples

• Dr. Edward Yeung (Iowa State Univ.) - Dr. Edward Yeung (Iowa State Univ.) - Nonlinear spectroscopy, laser-based detectors Nonlinear spectroscopy, laser-based detectors for liquid chromatography, capillary electrophoresis, single-cell and single-molecule analysis, for liquid chromatography, capillary electrophoresis, single-cell and single-molecule analysis, DNA sequencingDNA sequencing

A Clinical & Industrial Perspective: A Clinical & Industrial Perspective: The Industrial & Clinical Advisory Board (ICAB)The Industrial & Clinical Advisory Board (ICAB)

ICAB will provide feedback regarding the NCRB’s research ICAB will provide feedback regarding the NCRB’s research portfolio, programmatic activities and center direction from the portfolio, programmatic activities and center direction from the perspective of clinical laboratories and the perspective of clinical laboratories and the pharmaceutical/biotechnology industriespharmaceutical/biotechnology industries

This feedback will be used by the NCRB to develop contacts for This feedback will be used by the NCRB to develop contacts for the transition of methods to the biomedical community and the transition of methods to the biomedical community and provide an industrial/clinical perspective on critical needs for drug provide an industrial/clinical perspective on critical needs for drug discovery & biomarker detectiondiscovery & biomarker detection

The ICAB will meet with the Center Director and Mentoring The ICAB will meet with the Center Director and Mentoring Council annually in the spring, or six months prior to/following the Council annually in the spring, or six months prior to/following the meeting of the EACmeeting of the EAC

Industrial & Clinical Advisory Board (ICAB)Industrial & Clinical Advisory Board (ICAB)• Dr. Michael Grace Dr. Michael Grace ((Director of Bioanalytical Sciences, Bristol-Myers SquibbDirector of Bioanalytical Sciences, Bristol-Myers Squibb) - P) - Protein rotein

characterization, process analytical technology, biological interaction studies and the characterization, process analytical technology, biological interaction studies and the characterization of glycoconjugates.characterization of glycoconjugates.

• Dr. James E. McClurgDr. James E. McClurg ( (Senior Vice President and Chief Scientific Officer, MDS Senior Vice President and Chief Scientific Officer, MDS Pharma ServicesPharma Services) – Pharmaceutical analysis & related instrumentation) – Pharmaceutical analysis & related instrumentation

• Dr. John Masters Dr. John Masters ((Head of Discovery Chemistry in Research & Technology, Eli Lilly Head of Discovery Chemistry in Research & Technology, Eli Lilly and Companyand Company) - Medicinal chemistry, development of new analytical methods for ) - Medicinal chemistry, development of new analytical methods for screening of drugs and their metabolitesscreening of drugs and their metabolites

• Mr. Larry Middendorf Mr. Larry Middendorf ((Senior Vice President of Marketing/Sales, LI-COR Senior Vice President of Marketing/Sales, LI-COR BiosciencesBiosciences)).- .- Development and marketing of new tools and instruments for Development and marketing of new tools and instruments for bioanalysisbioanalysis

• Dr. Thomas Moyer Dr. Thomas Moyer ((Mayo Clinic;) - CMayo Clinic;) - Clinical chemist ry, toxicology and drug analysislinical chemist ry, toxicology and drug analysis

Facilities at UNL/UNMC in Area of Rapid Facilities at UNL/UNMC in Area of Rapid BioanalysisBioanalysis

Bioinformatics & Computational FacilitiesBioinformatics & Computational Facilities. . Methods and tools for traditional are available through existing Methods and tools for traditional are available through existing

facilities at UNL and UNMC (in collaboration with UNO)facilities at UNL and UNMC (in collaboration with UNO) More advanced computations and a cyberinfrastructure will be More advanced computations and a cyberinfrastructure will be

available through a new available through a new Computational CoreComputational Core

Instrument Development/MicrofabricationInstrument Development/Microfabrication Machine shop, and electronics shop are available at UNLMachine shop, and electronics shop are available at UNL Microfabrication can be conducted in the Nanofabrication Facility Microfabrication can be conducted in the Nanofabrication Facility

that is part of UNL’s Center for Materials Research and Analysisthat is part of UNL’s Center for Materials Research and Analysis

NanoimagingNanoimaging Facility available now at UNMCFacility available now at UNMC

Facilities at UNL/UNMC in Area of Rapid Facilities at UNL/UNMC in Area of Rapid Bioanalysis (Cont’d)Bioanalysis (Cont’d)

Structural & Functional AnalysisStructural & Functional Analysis Mass spectrometry - Nebraska Center for Mass Spectrometry Mass spectrometry - Nebraska Center for Mass Spectrometry

(UNL) & proteomics facility at UNMC(UNL) & proteomics facility at UNMC NMR spectroscopy – UNL (Dept. Chemistry) & UNMCNMR spectroscopy – UNL (Dept. Chemistry) & UNMC Functional analysis – Biacore & DNA arrays at both UNL & Functional analysis – Biacore & DNA arrays at both UNL &

UNMCUNMC Rapid structural analysis will be enhanced through the creation Rapid structural analysis will be enhanced through the creation

of a new of a new Robotics & Automated Sample Preparatio nCoreRobotics & Automated Sample Preparatio nCore Protein & Cell ProductionProtein & Cell Production

Facilties for protein expression, cell culturing & protein Facilties for protein expression, cell culturing & protein purification in UNL, Dept. Chemical & Biomolecular Engineeringpurification in UNL, Dept. Chemical & Biomolecular Engineering

Monoconal antibody production facility at UNMCMonoconal antibody production facility at UNMC

New Core Facilities Created by NCRBNew Core Facilities Created by NCRB

Computational Core Computational Core (Hui Li - Director, UNL)(Hui Li - Director, UNL)• Core will provide advanced computations and training Core will provide advanced computations and training

involving simulations and quantum chemical calculations involving simulations and quantum chemical calculations will be needed by some Center members (e.g., electronic will be needed by some Center members (e.g., electronic structure calculations of protein active sites, estimation of structure calculations of protein active sites, estimation of pKpKaa values, redox potentials, binding energies, solvation values, redox potentials, binding energies, solvation energies, NMR chemical shifts)energies, NMR chemical shifts)

• Core also will provide a cyberinfrastructure for the NCRB, Core also will provide a cyberinfrastructure for the NCRB, where researchers can readily exchange their data and link where researchers can readily exchange their data and link into programs and data generated by other Center facilitiesinto programs and data generated by other Center facilities

New Core Facilities Created by NCRB (Cont’d)New Core Facilities Created by NCRB (Cont’d)

Robotics & Automated Sample Preparation Core Robotics & Automated Sample Preparation Core (Joseph Dumais & Kurt Wulser - Directors, UNL)(Joseph Dumais & Kurt Wulser - Directors, UNL)

• Core will provide stations that will allow the automated Core will provide stations that will allow the automated preparation of samples for analysis by mass spectrometry preparation of samples for analysis by mass spectrometry and/or NMR spectroscopy and/or NMR spectroscopy

• Core will be housed near the MS and NMR facilities at UNLCore will be housed near the MS and NMR facilities at UNL

• Automated sample injection systems for the high-throughput Automated sample injection systems for the high-throughput analysis of samples by NMR and mass spectrometry will also analysis of samples by NMR and mass spectrometry will also be available through this Core be available through this Core

Other Components of Infrastructure Other Components of Infrastructure for the NCRBfor the NCRB

• Small grants program Small grants program

• Annual conference Annual conference

• Monthly seminar program Monthly seminar program

• Center membershipsCenter memberships

• WebsiteWebsite

Proposed Future Hires under NCRBProposed Future Hires under NCRB

• Junior level mass spectrometrist (proteomics, metabolomics or Junior level mass spectrometrist (proteomics, metabolomics or associated areas)associated areas) - Currently planned in Dept. Chemistry, UNL, under - Currently planned in Dept. Chemistry, UNL, under PoE in Functional Genomics PoE in Functional Genomics

• Junior level chemical engineer (biomolecular materials) Junior level chemical engineer (biomolecular materials) - - Planned by UNL Dept. Chemical & Biomolecular Engineering, UNLPlanned by UNL Dept. Chemical & Biomolecular Engineering, UNL

• Junior level analytical chemist (single molecule spectroscopy/ Junior level analytical chemist (single molecule spectroscopy/ bioanalytical spectroscopy) bioanalytical spectroscopy) - - Planned by Dept. Chemistry, UNLPlanned by Dept. Chemistry, UNL

• Junior level pharmaceutical chemist (drug Junior level pharmaceutical chemist (drug analysis/proteomics) - analysis/proteomics) - Planned by Dept. Pharmaceutical Science, Planned by Dept. Pharmaceutical Science, UNMCUNMC

• Junior level chemist/biochemist (glycomics, protein-protein Junior level chemist/biochemist (glycomics, protein-protein interactions or DNA-protein interactions) - interactions or DNA-protein interactions) - Planned by Dept. Planned by Dept. Chemistry, UNLChemistry, UNL

Task Quarter 1 Quarter 2 Quarter 3 Quarter 4

Administrator X

Mentoring Council Meetings X X X X

Internal Coordinating Committee Meetings X X

Website Development X X X

Monthly Seminar Series (X) X X X

Internal Coordinating Committee Meetings X X

External Advisory Committee Meetingc X (Year 2)

Industrial & Clinical Advisory Board Meeting X

Symposium X (Year 2)

Center Reviews X X

Formative Evaluation X (Year 2)

What will Occur During the Early Formation of What will Occur During the Early Formation of NCRB? (Year 1 - Quarter 1/Year 2)NCRB? (Year 1 - Quarter 1/Year 2)

Benchmark Year 1 Year 2 Year 3 Year 4 Year 5

Hire #1 (Mass Spectrometrist) X X

Hire #2 (Chemical Engineer) X X

Hire #3 (Analytical Chemist) X X

Hire #4 (Pharmaceutical Chemist) X X

Hire # 5 (Chemist/Biochemist) X X

Seed Grants X X X X

R01 Submission (Projects 1-5) (X)b X X

Project Turnover (Projects 1-5) (X)c X X

First P01 Submission (resubmission in Year 2, if needed)

X

Summative Evaluation (4th Quarter, Year 5) X

What will Occur after the Foundation of What will Occur after the Foundation of the NCRB? (Years 1 – 5)the NCRB? (Years 1 – 5)

What is Next for the NCRB COBRE Proposal?What is Next for the NCRB COBRE Proposal?

A final decision on funding of the current proposal is pending A final decision on funding of the current proposal is pending and expected in Spring 2009.and expected in Spring 2009.

Preparations are underway to help address comments in Preparations are underway to help address comments in review of previous proposal in preparation for resubmission review of previous proposal in preparation for resubmission later in 2009, if neededlater in 2009, if needed

Seed funds have been obtained from UNL & UNMC for Seed funds have been obtained from UNL & UNMC for Junior Project Leader to conduct preliminary studies to Junior Project Leader to conduct preliminary studies to address comments of their individual proposalsaddress comments of their individual proposals

Efforts to promote work and collaborations between UNL Efforts to promote work and collaborations between UNL and UNMC in the area of rapid bioanalysis, as evidenced by and UNMC in the area of rapid bioanalysis, as evidenced by this retreatthis retreat