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Page 1: Re producibility of glutamate and glutamine quantification ... · Guss 2. Harr 3. Nidd 4. Tsai 5. Hurd 6. Prov producibility tment of Electroni Taipei, Taiwan, 3Lab uate Institute

Re

1DeparTa

4Grad

Introd

and chthese (GABinformSpectrMRSIwhichcontriband th Metho

Medicconstaassessreprodparamto 8 slTEavgusing

LCMoquanti(Figurbetwe Result

were s(TableGln. Oand 10with Tassesssimila Refere1. Guss2. Harr3. Nidd4. Tsai 5. Hurd6. Prov

eproducibility

rtment of ElectroniTaipei, Taiwan, 3Labduate Institute of Ap

duction The cingulate c

hronic pain [1,2,conditions but A), the major in

mation about patroscopic imagingI protocol based h is in almost thebution of Gln an

he combination o

ods Sixteen healthy

cal Solutions, Erant short TE (TEsment of within-ducibility. Initial

meters were usedlices of outer vog, eight TEs wersingle average fStandard post p

odel [6]. Metaboified using the wre 1). The concen-week reprodu

ts and Discussio Representative similar for the twe 1). As expectedOverall, COV for0% for CC excepTEavg there wasment of short-tear performance o

ence sew A, NeuroImagris RE, Arthritis Rhdam DM, Am J GaSY, MRM, 2008

d R, MRM, 2004 vencher SW, MRM

FigurThe Rmedia(red) (greensubjec

y of glutamate

ic Engineering, Naboratory of Integrpplied Physics, Na

ortex (CC) in th,3]. As the majoalso as a mechanhibitory neurotthological mechg (MRSI) methoon the PEPSI s

e same spectral rnd in turn enhanof Glu and Gln (G

subjects (7 malrlangen, GermanE30) and (2) TEday reproducibily, a high-solutio: sagital PEPSI

olume lipid supprre used from 35for automatic phaprocessing was polite concentratiowater-scaling mcentrations and ucibility of TE30

on maps of metabwo protocols exd, smaller CRLBr TE30 were lesspt for Gln (~20%as no obvious derm and long-teron Glu but provid

ge,2011 heum., 2009 astro., 2011

M, 1993

re1 ROIs of al wall and CC n) of one ct

and glutamin

Woan-Chyi Wational Taiwan Unrated Brain Researational Chengchi U

he medial wall ofor excitatory neuanism for drug ransmitter of thanisms and drug

od that can be ussequence to deterange as Gln usince Glu in the spGlx) was evalua

les/ 9 females; mny) with a 32-chE average (TEality. Eight subjeon 3D T1 imageacquisition; 14mression were app ms to 185 ms wase correction anperformed for thons of N-Acetyl

method. ROIs of Cramer-Rao Lo0 and TEavg pro

olite concentratixcept for Gln, GB for Glu and higs than 5% for the

%), which is in adifference in Glurm changes in bdes more accurat

ne quantificati

Wang1, Yi-Ru Lin1, Dniversity of Sciencerch, Department ofUniversity, Taipei,

T

f the brain is invurotransmitter of

intervention. Ge brain. Quantifg dynamics. Prosed to assess the ect Glu and Glning 1H MRS, a spectra [5]. Using

ated with short-T

mean±SD: 29.9±hannel head coiavg). All subjecects returned for e was obtained fomm slice thickneplied along the pwith 20 ms incrnd calibration ofhe PEPSI data aAspartate (NAA

f the entire medower Bound (Cotocols were asse

ions and CRLB Glx and mI (Tablgher CRLB for Ge medial wall anccordance with tu COV betweenbrain metabolitete quantification

Figure2Concentration mand CRLB mapmetabolites fromTE30 protocol

ion in the cingimaging

David M. Niddame and Technology, f Medical ResearcTaiwan, 5Reasear

Taipei, Taiwan

volved in a widef the brain, glutalutamine (Gln)fication of the Goton Echo Plana

distribution of b in CC in less th

spectral editing mg this protocol,

TE PEPSI and TE

±8.2; range: 21 tol array. The repts were scannedthe same experi

or localization oess; 8 x 8 mm2 inperimeter of the rements. An addf metabolic concas described pre

A), total Creatineial wall and CCRLB) of all messed by the coe

from one subjele 1). The CRLBGln were found wnd 8% for CC (Tthe CRLB resultn the two protocs of the medial

n of Gln and othe

maps ps of 7 m Table 1 T

concentra

gulate cortex u

m2,3, and Shang-YueTaipei, Taiwan, 2B

ch and Education, rch Center for Min

e range of patholamate (Glu) playis the precursor

Glu and Gln metar Spectroscopy brain metabolitethan 10 minutesmethod based onthe reproducibil

Eavg PEPSI.

o 49 years;) werproducibility of d twice on the iment around 2

of the medial wan-plane resolutiobrain. TE30 par

ditional non-watecentrations. eviously [4]. Afe (tCr), Choline (C were manually

metabolites were efficient of varian

ect are shown inB for NAA, tCrwith TEavg indi

Table 2). For TEats. It is notewortcols. In concluswall. Compared

er metabolites.

The average and station and CRLB fo

using proton e

eh Tsai4,5 Brain Research CeTaipei Veterans G

nd, Brain and Lear

logical conditionys an important r of both Glu atabolism in CC Imaging (PEPS

es [4]. In this stu. To further impn TE averaging lity of the metab

re scanned on a MRSI was evalsame day (withweeks later for ll and CC in theon; FOV=256 x rameters were Ter suppression (

fter reconstructio(Cho), myo-Inosy selected on thcalculated for

nce (COV).

n Figure 2. The r, and Cho wereicating successfuavg, COVs werethy that althoughsion, the small d to the TEavg

tandard deviation oor all 16 subjects.

echo planar sp

enter, National YanGeneral Hospital, Trning, National Ch

ns including psyrole not only in

and Gamma-Ammay therefore p

SI) is a fast Magudy, we proposeprove the detecti(TEavg) were ubolite concentrat

3T MR system luated by 2 PEPhout leaving the

the assessment e right hemispher

256 mm2; and TTE = 30 ms and (NWS) PEPSI sc

on, spectra weresitol (mI), Glu, Ghe individual aneach ROI. The

mean metabolie less than 8% foul spectral editine less than 6% foh a better fit wasCOVs make PEprotocol, the TE

of Table 2 Withinweek COV of b

pectroscopic

ng-Ming UniversityTaipei, Taiwan, hengchi University

ychiatric disorderthe pathology o

mino Butyric aciprovide importangnetic Resonanca 2-diemensionaion of Glu alone

used to reduce thtions of Glu, Gl

(Trio, SIEMENPSI protocols: (1

scanner) for thof between-weere. The followinTR=1500 ms. UNEX = 8 and focan was acquire

e quantified witGln and Glx wernatomical imagee within-day an

ite concentrationfor both protocolng/ suppression oor the medial was obtained for GlEPSI suitable foE30 protocol ha

n-day and betweenboth protocols.

y,

y,

rs of id nt ce al e,

he ln

S 1) he ek ng Up or ed

th re es nd

ns ls of ll lu or ad

-

1719Proc. Intl. Soc. Mag. Reson. Med. 20 (2012)