Re producibility of glutamate and glutamine quantification ... · Guss 2. Harr 3. Nidd 4. Tsai 5....
1
Re 1 Depar T a 4 Grad Introd and ch these (GAB inform Spectr MRSI which contrib and th Metho Medic consta assess reprod param to 8 sl TEavg using LCMo quanti (Figur betwe Result were s (Table Gln. O and 10 with T assess simila Refere 1. Guss 2. Harr 3. Nidd 4. Tsai 5. Hurd 6. Prov eproducibility rtment of Electroni aipei, Taiwan, 3 Lab duate Institute of Ap duction The cingulate c hronic pain [1,2, conditions but A), the major in mation about pat roscopic imaging I protocol based h is in almost the bution of Gln an he combination o ods Sixteen healthy cal Solutions, Er ant short TE (TE sment of within- ducibility . Initial meters were used lices of outer vo g, eight TEs wer single average f Standard post p odel [6]. Metabo ified using the w re 1). The conc en-week reprodu ts and Discussio Representative similar for the tw e 1). As expected Overall, COV for 0% for CC excep TEavg there wa sment of short-te ar performance o ence sew A, NeuroImag ris RE, Arthritis Rh dam DM, Am J Ga SY, MRM, 2008 d R, MRM, 2004 vencher SW, MRM Figur The R media (red) (green subjec y of glutamate ic Engineering, Na boratory of Integr pplied Physics, Na ortex (CC) in th ,3]. As the majo also as a mecha nhibitory neurot thological mech g (MRSI) metho on the PEPSI s e same spectral r nd in turn enhan of Glu and Gln (G subjects (7 mal rlangen, German E30) and (2) TE day reproducibi ly , a high-solutio : sagital PEPSI olume lipid suppr re used from 35 or automatic pha processing was p olite concentratio water-scaling m centrations and ucibility of TE30 on maps of metab wo protocols ex d, smaller CRLB r TE30 were less pt for Gln (~20% as no obvious d erm and long-ter on Glu but provid ge,2011 heum., 2009 astro., 2011 M, 1993 re1 ROIs of al wall and CC n) of one ct and glutamin Woan-Chyi W ational Taiwan Un ated Brain Resear ational Chengchi U he medial wall of or excitatory neu anism for drug ransmitter of th anisms and drug od that can be us sequence to dete range as Gln usi nce Glu in the sp Glx) was evalua les/ 9 females; m ny) with a 32-ch E average (TEa lity. Eight subje on 3D T1 image acquisition; 14m ression were app ms to 185 ms w ase correction an performed for th ons of N-Acetyl method. ROIs of Cramer-Rao Lo 0 and TEavg pro olite concentrati xcept for Gln, G B for Glu and hig s than 5% for the %), which is in a difference in Glu rm changes in b des more accurat ne quantificati Wang 1 , Yi-Ru Lin 1 , D niversity of Science rch, Department of University, Taipei, T f the brain is inv urotransmitter of intervention. G e brain. Quantif g dynamics. Pro sed to assess the ect Glu and Gln ing 1 H MRS, a s pectra [5]. Using ated with short-T mean±SD: 29.9± hannel head coi avg). All subjec ects returned for e was obtained fo mm slice thickne plied along the p with 20 ms incr nd calibration of he PEPSI data a Aspartate (NAA the entire med ower Bound (C otocols were asse ions and CRLB Glx and mI (Tabl gher CRLB for G e medial wall an ccordance with t u COV between brain metabolite te quantification Figure2 Concentration m and CRLB map metabolites from TE30 protocol ion in the cing imaging David M. Niddam e and Technology, f Medical Researc Taiwan, 5 Reasear T aipei, Taiwan volved in a wide f the brain, gluta lutamine (Gln) fication of the G oton Echo Plana distribution of b in CC in less th spectral editing m g this protocol, TE PEPSI and TE ±8.2; range: 21 to l array . The rep ts were scanned the same experi or localization o ess; 8 x 8 mm 2 in perimeter of the rements. An add f metabolic conc as described pre A), total Creatine ial wall and CC RLB) of all m essed by the coe from one subje le 1). The CRLB Gln were found w nd 8% for CC (T the CRLB result n the two protoc s of the medial n of Gln and othe maps ps of 7 m Table 1 T concentra gulate cortex u m 2,3 , and Shang-Yue Taipei, Taiwan, 2 B ch and Education, rch Center for Min e range of pathol amate (Glu) play is the precursor Glu and Gln met ar Spectroscopy brain metabolite than 10 minutes method based on the reproducibil Eavg PEPSI. o 49 years;) wer producibility of d twice on the iment around 2 of the medial wa n-plane resolutio brain. TE30 par ditional non-wate centrations. eviously [4]. Af e (tCr), Choline ( C were manually metabolites were efficient of varian ect are shown in B for NAA, tCr with TEavg indi T able 2). For TEa ts. It is notewort cols. In conclus wall. Compared er metabolites. The average and st ation and CRLB fo using proton e eh Tsai 4,5 Brain Research Ce Taipei Veterans G nd, Brain and Lear logical condition ys an important r of both Glu a tabolism in CC Imaging (PEPS es [4]. In this stu . To further imp n TE averaging lity of the metab re scanned on a MRSI was eval same day (with weeks later for ll and CC in the on; FOV=256 x rameters were T er suppression ( fter reconstructio (Cho), myo-Inos y selected on th calculated for nce (COV). n Figure 2. The r , and Cho were icating successfu avg, COVs were thy that although sion, the small d to the TEavg tandard deviation o or all 16 subjects. echo planar sp enter, National Yan General Hospital, T rning, National Ch ns including psy role not only in and Gamma-Am may therefore p SI) is a fast Mag udy , we propose prove the detecti (TEavg) were u bolite concentrat 3T MR system luated by 2 PEP hout leaving the the assessment e right hemispher 256 mm 2 ; and T TE = 30 ms and (NWS) PEPSI sc on, spectra were sitol (mI), Glu, G he individual an each ROI. The mean metaboli e less than 8% fo ul spectral editin e less than 6% fo h a better fit was COVs make PE protocol, the TE of Table 2 Within week COV of b pectroscopic ng-Ming Universit T aipei, Taiwan, hengchi University ychiatric disorder the pathology o mino Butyric aci provide importan gnetic Resonanc a 2-diemensiona ion of Glu alone used to reduce th tions of Glu, Gl (Trio, SIEMEN PSI protocols: (1 scanner) for th of between-wee re. The followin TR=1500 ms. U NEX = 8 and fo can was acquire e quantified wit Gln and Glx wer natomical image e within-day an ite concentration or both protocol ng/ suppression o or the medial wa s obtained for Gl EPSI suitable fo E30 protocol ha n-day and between both protocols. y, y, rs of id nt ce al e, he ln S 1) he ek ng Up or ed th re es nd ns ls of ll lu or ad - 1719 Proc. Intl. Soc. Mag. Reson. Med. 20 (2012)
Transcript of Re producibility of glutamate and glutamine quantification ... · Guss 2. Harr 3. Nidd 4. Tsai 5....
Re
1DeparTa
4Grad
Introd
and chthese (GABinformSpectrMRSIwhichcontriband th Metho
Medicconstaassessreprodparamto 8 slTEavgusing
LCMoquanti(Figurbetwe Result
were s(TableGln. Oand 10with Tassesssimila Refere1. Guss2. Harr3. Nidd4. Tsai 5. Hurd6. Prov
eproducibility
rtment of ElectroniTaipei, Taiwan, 3Labduate Institute of Ap
duction The cingulate c
hronic pain [1,2,conditions but A), the major in
mation about patroscopic imagingI protocol based h is in almost thebution of Gln an
he combination o
ods Sixteen healthy
cal Solutions, Erant short TE (TEsment of within-ducibility. Initial
meters were usedlices of outer vog, eight TEs wersingle average fStandard post p
odel [6]. Metaboified using the wre 1). The concen-week reprodu
ts and Discussio Representative similar for the twe 1). As expectedOverall, COV for0% for CC excepTEavg there wasment of short-tear performance o
ence sew A, NeuroImagris RE, Arthritis Rhdam DM, Am J GaSY, MRM, 2008
d R, MRM, 2004 vencher SW, MRM
FigurThe Rmedia(red) (greensubjec
y of glutamate
ic Engineering, Naboratory of Integrpplied Physics, Na
ortex (CC) in th,3]. As the majoalso as a mechanhibitory neurotthological mechg (MRSI) methoon the PEPSI s
e same spectral rnd in turn enhanof Glu and Gln (G
subjects (7 malrlangen, GermanE30) and (2) TEday reproducibily, a high-solutio: sagital PEPSI
olume lipid supprre used from 35for automatic phaprocessing was polite concentratiowater-scaling mcentrations and ucibility of TE30
on maps of metabwo protocols exd, smaller CRLBr TE30 were lesspt for Gln (~20%as no obvious derm and long-teron Glu but provid
ge,2011 heum., 2009 astro., 2011
M, 1993
re1 ROIs of al wall and CC n) of one ct
and glutamin
Woan-Chyi Wational Taiwan Unrated Brain Researational Chengchi U
he medial wall ofor excitatory neuanism for drug ransmitter of thanisms and drug
od that can be ussequence to deterange as Gln usince Glu in the spGlx) was evalua
les/ 9 females; mny) with a 32-chE average (TEality. Eight subjeon 3D T1 imageacquisition; 14mression were app ms to 185 ms wase correction anperformed for thons of N-Acetyl
method. ROIs of Cramer-Rao Lo0 and TEavg pro
olite concentratixcept for Gln, GB for Glu and higs than 5% for the
%), which is in adifference in Glurm changes in bdes more accurat
ne quantificati
Wang1, Yi-Ru Lin1, Dniversity of Sciencerch, Department ofUniversity, Taipei,
T
f the brain is invurotransmitter of
intervention. Ge brain. Quantifg dynamics. Prosed to assess the ect Glu and Glning 1H MRS, a spectra [5]. Using
ated with short-T
mean±SD: 29.9±hannel head coiavg). All subjecects returned for e was obtained fomm slice thickneplied along the pwith 20 ms incrnd calibration ofhe PEPSI data aAspartate (NAA
f the entire medower Bound (Cotocols were asse
ions and CRLB Glx and mI (Tablgher CRLB for Ge medial wall anccordance with tu COV betweenbrain metabolitete quantification
Figure2Concentration mand CRLB mapmetabolites fromTE30 protocol
ion in the cingimaging
David M. Niddame and Technology, f Medical ResearcTaiwan, 5Reasear
Taipei, Taiwan
volved in a widef the brain, glutalutamine (Gln)fication of the Goton Echo Plana
distribution of b in CC in less th
spectral editing mg this protocol,
TE PEPSI and TE
±8.2; range: 21 tol array. The repts were scannedthe same experi
or localization oess; 8 x 8 mm2 inperimeter of the rements. An addf metabolic concas described pre
A), total Creatineial wall and CCRLB) of all messed by the coe
from one subjele 1). The CRLBGln were found wnd 8% for CC (Tthe CRLB resultn the two protocs of the medial
n of Gln and othe
maps ps of 7 m Table 1 T
concentra
gulate cortex u
m2,3, and Shang-YueTaipei, Taiwan, 2B
ch and Education, rch Center for Min
e range of patholamate (Glu) playis the precursor
Glu and Gln metar Spectroscopy brain metabolitethan 10 minutesmethod based onthe reproducibil
Eavg PEPSI.
o 49 years;) werproducibility of d twice on the iment around 2
of the medial wan-plane resolutiobrain. TE30 par
ditional non-watecentrations. eviously [4]. Afe (tCr), Choline (C were manually
metabolites were efficient of varian
ect are shown inB for NAA, tCrwith TEavg indi
Table 2). For TEats. It is notewortcols. In concluswall. Compared
er metabolites.
The average and station and CRLB fo
using proton e
eh Tsai4,5 Brain Research CeTaipei Veterans G
nd, Brain and Lear
logical conditionys an important r of both Glu atabolism in CC Imaging (PEPS
es [4]. In this stu. To further impn TE averaging lity of the metab
re scanned on a MRSI was evalsame day (withweeks later for ll and CC in theon; FOV=256 x rameters were Ter suppression (
fter reconstructio(Cho), myo-Inosy selected on thcalculated for
nce (COV).
n Figure 2. The r, and Cho wereicating successfuavg, COVs werethy that althoughsion, the small d to the TEavg
tandard deviation oor all 16 subjects.
echo planar sp
enter, National YanGeneral Hospital, Trning, National Ch
ns including psyrole not only in
and Gamma-Ammay therefore p
SI) is a fast Magudy, we proposeprove the detecti(TEavg) were ubolite concentrat
3T MR system luated by 2 PEPhout leaving the
the assessment e right hemispher
256 mm2; and TTE = 30 ms and (NWS) PEPSI sc
on, spectra weresitol (mI), Glu, Ghe individual aneach ROI. The
mean metabolie less than 8% foul spectral editine less than 6% foh a better fit wasCOVs make PEprotocol, the TE
of Table 2 Withinweek COV of b
pectroscopic
ng-Ming UniversityTaipei, Taiwan, hengchi University
ychiatric disorderthe pathology o
mino Butyric aciprovide importangnetic Resonanca 2-diemensionaion of Glu alone
used to reduce thtions of Glu, Gl
(Trio, SIEMENPSI protocols: (1
scanner) for thof between-weere. The followinTR=1500 ms. UNEX = 8 and focan was acquire
e quantified witGln and Glx wernatomical imagee within-day an
ite concentrationfor both protocolng/ suppression oor the medial was obtained for GlEPSI suitable foE30 protocol ha
n-day and betweenboth protocols.
y,
y,
rs of id nt ce al e,
he ln
S 1) he ek ng Up or ed
th re es nd
ns ls of ll lu or ad
-
1719Proc. Intl. Soc. Mag. Reson. Med. 20 (2012)
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