0 1 2 3 4 5 6 7 8 9 10 11 20 21 60 60
360 514 254 160 100 69 44 34 18 18 7 23 14 11 5
Detection of Human Coronavirus in Fukui Prefecture
Eiko IGARASHI, Kaori SATO, Taeko SAKAI, Miho TOHO
2003 SARSSevere Acute Respiratory Syndrome 2011
MERS Middle East Respi-ratory Syndrome coronavirus
HCoVHCoV HCoV-OC43 HCoV-229E HCoV-
HKU1 HCoV-NL63 HCoV 15 35 1)
2) 53)
Respira- tory Syncytial HRSV
HBoV 54)
2010 20130.4%
5) HCoVHCoV
HCoVHCoV
2013 1 2016 12
1,631
2013 276 2014 4902015 501 2016 364
0 93 3.5 7.51.8 1
3,000rpm 10 QIAamp Viral RNA Mini Kit QIAGEN
Primescript RT reagent kit Takara Bio229E6) OC436) NL637)
HKU18) ExTaq Takara Bio PCR
MinElute PCR purification kit QIAGEN BigDye Terminator v3.1 Cycle Sequencing Kit Applied Biosystems
BigDye XTerminator® Purification Kit Applied Biosystems ABI PRISM 3130 Genetic Analyzer Applied Biosystems
BLAST
HCoV
1,631 30 HCoV1.8%
7.6 %5) 2.3%8)
1 2 3 4 5 6 7 8 9 10 11 12 total
2013 0 0 0 0 1 0 0 0 0 0 0 1 2
2014 0 1 2 2 2 0 0 1 0 0 0 4 12
2015 6 6 2 0 0 0 0 0 0 0 0 1 15
2016 0 0 0 0 0 0 0 0 0 0 1 0 1
total 6 7 4 2 3 0 0 1 0 0 1 6 30
HCoV 1 5 8 11 12 13 12 HCoV
5)
HCoV 2014
20152
HCoV 30 1 229E 10OC43 14 NL63 5 HKU1
HCoV OC43 NL63 HKU1 229E8),9) NL63
OC43 2013 2015 2016 1 2
5 11 12 NL63 20142015 1 4 8 12HKU1 2014 3 5 2015 12
1 229E
5) 1 3.3%
OC43 2013 1 4 10) 5)
2013 5 121
2015 1 2 92015 1 4
11) 2014 12 2015 4 OC439)
OC43 NL63 2014 12 2015 3 11
2015 2 5 12)
HKU1 2010 2012
5) 20142015 1
HCoV HCoV 0 5 7 222.9 4.0 30 28 5
0 3 1 3 21 3 1
2.3 1.8 HCoV 52)HBoV 1.4
0.8 13) 229E 1 HKU1 0 3
NL63 0 5 7 22 OC430 3 5 2 HKU1
0 4 5 OC43 3 4 611 NL63 10
HCoV 6 0
2 1 10)
2016 6 0-112)
HCoV
HCoV HCoV 30
17 133 2010 2011 HCoV 17
4 1314)
HCoV 229E 1
OC43 10 64 NL63
14 8 6HKU1 5 2 3
OC43 4
0 OC43
HCoVNL63 15) OC43
16)
NL63 OC43 HKU1
HCoV
2013 2017
HCoV1,631 30
HCoV
HCoV
1) Li W. et al. The S proteins of human coronavirus
NL63 and severe acute respiratory syndrome coronavirus bind overlapping regions of ACE2, Virology, 2, 367 374(2007)
2) : , , (2011)
3) MERS, , 60(4), 137-142(2014)
4) (2010 ),
, 9, 93-96 (2010) 5) Matoba Y. et al. Detection of the Human
Coronavirus 229E, HKU1, NL63, and OC43 between 2010 and 2013 in Yamagata, Japan, Jpn. I. Infect. Dis., 68,138-141(2015)
6) Vabret A. et al. Direct diagnosis of human respiratory coronaviruses 229E and OC43 by the polymerase chain reaction, J. Virological Methods, 97 ,59-66 (2001)
7) Vabret A. et al. Human Coronavirus NL63, France, Emer. Infect. Dis., 11, 1225-1229(2005)
8) Guant E.R. et al. Epidemiology and clinical presentations of the four human coronaviruses 229E, HKU1, NL63, and OC43 detected over 3 years using a novel multiplex real-time PCR method, J. Clin. Microbiol., 48, 2940-2947(2010)
9) IASR https://www.niid.g o.jp/niid/images/iasr/archive/2016/virus/infl1116.pdf
10) HCoV-OC43 ,
, 88(5), 708-710(2015) 11) Matoba Y. et al. An outbreak of Human
Coronavirus OC43 during the 2014-2015 influenza Season in Yamagata, Japan, Jpn. I. Infect. Dis., 68, 442-445(2015)
12) Matoba Y. et al. Trends of Human Coronaviruses in Yamagata, Japan in 2015-2016 Focusing on the OC43 Outbreak of June 2016, Jpn. I. Infect. Dis., 71, 167-169(2018)
13) , , 12, 72-75(2013)
14) Kon M. et al. Detection of human coronavirus NL63 and OC43 in children with acute respiratory infections in Niigata, Japan, between 2010 and 2011, Jpn. I. Infect. Dis., 65, 270-272(2012)
15) NL63 ,
, 29(1), 61-66(2017) 16) Epidemiology and clinical characteristics of human
coronaviruses OC43, 229E, NL63, and HKU1: a study of hospitalized children with acute respiratory tract infection in Guangzhou, China, Eur. J. Microbiol. Infect. Dis., 37, 353-369(2018)
2
Study of Simultaneous Analysis Method for Fusarium Toxin (2nd Report)
Yasuyuki SAKAI, Ikue KONISHI
1,2)
3,4)
LC-MS/MS
LC-MS/MSMRM
5) LC-MS/MS
3-15-
-X HT-2 T-2
DON NIV 3-AcDON 15-AcDON FUXHT-2 T-2 DAS ZEN
Merck
1.0mg10mL 100ppm
1mL10mL
10ppm
VEL ZAN13C-
13C-DON Biopure 25ppm
5.0mg50mL 100ppm
25 L25ppm13C-DON 100 L
250 L 10ppm
LC/MS
Romer MultiSep226MultiSep227 Mycospin400 GLInertSep VRA-1 InertSep VRA-3Autoprep MF-T1500 Merck SupelTox AflaZeaSupelTox Tricho Agilent BondElut Mycotoxin
GL InertSep PLR
Agilent Captiva EMR- Lipid Merck Z-Sep
Prominence 20A/3200Q TRAP /Sciex CAPCELL CORE ADME 2.7 m 2.1mm i.d. 150mm (
A 5mM B5mM
B 10%(0min) 90%(15min) 90%(20min)0.2mL/min 40 5 L MRM
1 LC-MS/MS
100ng/mL
ESI Positive 3-AcDON 15-AcNIV FUX HT-2 T-2 339>231 356>321 372>355 442>263 484>305 339>203 356>137 372>247 442>215 484>185
26,7,8)
ESI Negative
DON NIV VEL 13C-DON
371>281 355>295 325>59 370>310 371>311 355>265 370>279
ESI Positive
DAS VEL ZEN ZAN
384>307 284>267 319>187 321>303 384>229 284>249 319>283 321>189
1 9)
1
2
40 90% 20%90%
80130%
1 10%
3 Negative DON
NIVPositive
100
Positive 100
IQL DONNIV 3-AcDON 15-AcDON FUX ZEN 10ng/mLHT-2 T-2 DAS 1ng/mL
MQLIQL 100
1MultiSep 226 2
MycoSpin400 1mL10,000rpm 1min
0.4mL 10%0.1mL
GL InertSep VRA-1InertSep VRA-3
Romer MultiSep226MultiSep227MycoSpin400 Autoprep MF-T1500
Merck SupelTox AflaZeaSupelTox Tricho
Agilent BondElut Mycotoxin
4 MultiSep 226
MultiSep 227 Autoprep MF-T1500 SupelTox AflaZeaZEN
ZENMultiSep
226MultiSep 226
MultiSep 226
3 MultiSep 226
5
EMR85%
50% 75%
(1) (2)
(3)
6
Negative 13C-DONNIV
DON 13C-DONVEL
Positive ZEN ZANVEL Negative
n=37 30 110%
70 120%Negative DON NIV
Positive
Negative
PositiveVEL ZEN
VEL ZAN
NegativeDON NIV Positive
Negative Positive
VEL ZAN
1) 2015 65. ,562-647,
, (2015) 2) 2015 2.2
,287-305, , (2015) 3)
13
,98-113(2001) 4)
14
,6-9(2002) 5)
,15,72-77(2016) 6) ,53(3),152-156(2012) 7) Yoshinari et al. Journal of Agricultural and Food
Chemistry, 62(5),1174-1180(2014) 8) 20 4 1 19 14729
28 12 22 9) 14 5 21 0521001
157 17
Distribution of Phosphate Ester Flame Retardants in River Water in Fukui.
Takahiro YAMAZAKI, Masanori YOSHIKAWA
HBCD C12H18Br6 26 3
HBCD
9
30 32
200mL
10 L 20mL10mL
OASIS-HLB-Plus20mL
3000rpm 10min8mL
1mLMILLIPORE Millex-LG
LC-MS/MS2 3 4 LC-MS/MS
1)
A B
d-
200ml
N2
8ml
N2 <0.2ml
LC-MS/MS
10ng
183 99225 99267 99
2- 285 632- 327.3 99
327.5 1522- 420 3211,3- -2- 431 99
369 91
198 102246 102294 102
2- 297 672- 345 102
342 822- 433 3301,3- -2- 446 102
390 98
B 10 53 /min 2
4 B30 5 2.8 /min3
n=4 581 118 70
120 %
6 90.20 3.5ng/L
4 30 3229 10 11 30 2 3
85 2
870 ng/L
HBCDHBCD
7,8
HBCD
2- HBCD
HBCD
LC-MS/MS LP-8050CORETECSTM
A 0.1 B 0 5 min A 70 B 30 5 30 min A 70 0 B 30 100 0.2 mL/min 40
[ ]101 11781 116109 11790 9999 11797 11891 10289 10695 108
[ng/L]
0.30 0.950.55 1.80.40 1.4
2- 3.5 122- 0.45 1.5
0.75 2.52- 0.85 2.91,3- -2- 0.90 3.0
0.20 0.75
9
30 32
8
29
1) -LC-MS/MS
,(2017)
HBCD6
9 50 017 5
2- 12 42- 26 6
22 22- 14 51,3- -2- 14 5
12 1
32
HBCD6
22 60 013 5
2- 18 42- 29 5
13 32- 25 51,3- -2- 7 4
21 5
31
2
*1 *2 *3
Development of Basic System Aiming at Stabilization of Waste Landfill
by Pumping Circulation of Leachate (Part 2)
Hirokazu TANAKA, Masamitsu NAKAMURA, Yugo ISOBE* ,
Masatoshi TSUBAKI* , Kazuo KAMURA*3
1-10)
11-15)
2012 2013
16,17)
1
4 m1 m
VP250.5 m
1.55 m 20 L
1.1 m 0.3 MPa 20 L min-1 0.2 MPa
EC (VP25)
2 20 m5 m
107 m 16,17)
6 L min-1
350m 16,18) 90 GL-0.2 m
2009 1.3 m3.11 10-3 cm s-1
*1
*2
*3
2016 3 16 2017 1 312016 8 17 9 9 10 27
11 4
24
1 5 1 2016 10 26 2
- 1 m
3
2.0 m3 6.3 L 16.9 L 71
62 m3 16,17)
3.2 % 10.7 %
15 m4
GL 2 m
1.2 m 1 m
24
EC 5
8 8 17 9 99
12
EC
EC 12
19,20)
EC16,17)
62014
Line-A (2016)4 m
2014 Line-B (2016)4 m 2014
EC5 EC
Line-A (2016)2014
Line-C (2016)
Line-A (2016) 24 m
Line-C (2016) 1 3 mLine-A (2016)
16,17)
EC
ECLine-A Line-C
1
EC
1
1) , , :
, 15,1255-1257(2004)
2)Kamura Kazuo, Hara Yu, Bulent Inac ,Yamada Masato, Inoue Yuzo, Ono Yusaku: Effectiveness of resistivity monitoring for interpreting temporal changes in landfill properties, Journal of material cycles and waste management,7(2), 66-70(2005) 3) , , :
, 19,753-755(2008)
4) , , , , :
, 2010,77-82(2010)
5)Kamura Kazuo, Okuno Manami, Sakurama Toshinori, Wakabayashi Kyoko, Oyama Kenya, Tanaka Hirokazu : Metal enrichment processes and exploration of enriched zones in landfill sites, Proceedings of ISWA World Congress (ISBN978-3-200-03229-3), Electronic file(2013) 6)Kamura Kazuo, Ito Takahiro, Yokose Ryuji, KurokawaMasahiro. Takeuchi Mutsuo,Tanaka,Hirokazu:Exploration of metal-enriched zones in landfills,Proceedings of IWWG-ARB2015,123-128(2015) 7) , , , , :
, 133 ,1-4(2015)
8) , , , , ,: ,
134 ,195-198(2016) 9) , , :
, ,27(1), 27-38(2016)
10) , , , , ,:
, 69(3),173-183(2016) 11) 21
- , ,, ,36(14),1222-1225(2000)
12) ,,
,58(268),544-550(2005) 13)
-, ,32(11),17-21(2006)
14) vol.6 ,77,86-92(2008)
15) , :, , 20(6),
308-313(2009) 16) , , , :
,,12,80-83(2013)
17) , , , , , ,, , , :
, 24,501-502(2013)
18) -http://suidoubox.net/suiribasic17.html
2014.06.16 19) , , , , , ,
: , 36,291-293(2015)
20) , : ,,13,63-66(2014)
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