0 1 2 3 4 5 6 7 8 9 10 11 20 21 60 60

360 514 254 160 100 69 44 34 18 18 7 23 14 11 5

Detection of Human Coronavirus in Fukui Prefecture

Eiko IGARASHI, Kaori SATO, Taeko SAKAI, Miho TOHO

2003 SARSSevere Acute Respiratory Syndrome 2011

MERS Middle East Respi-ratory Syndrome coronavirus

HCoVHCoV HCoV-OC43 HCoV-229E HCoV-

HKU1 HCoV-NL63 HCoV 15 35 1)

2) 53)

Respira- tory Syncytial HRSV

HBoV 54)

2010 20130.4%

5) HCoVHCoV

HCoVHCoV

2013 1 2016 12

1,631

2013 276 2014 4902015 501 2016 364

0 93 3.5 7.51.8 1

3,000rpm 10 QIAamp Viral RNA Mini Kit QIAGEN

Primescript RT reagent kit Takara Bio229E6) OC436) NL637)

HKU18) ExTaq Takara Bio PCR

MinElute PCR purification kit QIAGEN BigDye Terminator v3.1 Cycle Sequencing Kit Applied Biosystems

BigDye XTerminator® Purification Kit Applied Biosystems ABI PRISM 3130 Genetic Analyzer Applied Biosystems

BLAST

HCoV

1,631 30 HCoV1.8%

7.6 %5) 2.3%8)

1 2 3 4 5 6 7 8 9 10 11 12 total

2013 0 0 0 0 1 0 0 0 0 0 0 1 2

2014 0 1 2 2 2 0 0 1 0 0 0 4 12

2015 6 6 2 0 0 0 0 0 0 0 0 1 15

2016 0 0 0 0 0 0 0 0 0 0 1 0 1

total 6 7 4 2 3 0 0 1 0 0 1 6 30

HCoV 1 5 8 11 12 13 12 HCoV

5)

HCoV 2014

20152

HCoV 30 1 229E 10OC43 14 NL63 5 HKU1

HCoV OC43 NL63 HKU1 229E8),9) NL63

OC43 2013 2015 2016 1 2

5 11 12 NL63 20142015 1 4 8 12HKU1 2014 3 5 2015 12

1 229E

5) 1 3.3%

OC43 2013 1 4 10) 5)

2013 5 121

2015 1 2 92015 1 4

11) 2014 12 2015 4 OC439)

OC43 NL63 2014 12 2015 3 11

2015 2 5 12)

HKU1 2010 2012

5) 20142015 1

HCoV HCoV 0 5 7 222.9 4.0 30 28 5

0 3 1 3 21 3 1

2.3 1.8 HCoV 52)HBoV 1.4

0.8 13) 229E 1 HKU1 0 3

NL63 0 5 7 22 OC430 3 5 2 HKU1

0 4 5 OC43 3 4 611 NL63 10

HCoV 6 0

2 1 10)

2016 6 0-112)

HCoV

HCoV HCoV 30

17 133 2010 2011 HCoV 17

4 1314)

HCoV 229E 1

OC43 10 64 NL63

14 8 6HKU1 5 2 3

OC43 4

0 OC43

HCoVNL63 15) OC43

16)

NL63 OC43 HKU1

HCoV

2013 2017

HCoV1,631 30

HCoV

HCoV

1) Li W. et al. The S proteins of human coronavirus

NL63 and severe acute respiratory syndrome coronavirus bind overlapping regions of ACE2, Virology, 2, 367 374(2007)

2) : , , (2011)

3) MERS, , 60(4), 137-142(2014)

4) (2010 ),

, 9, 93-96 (2010) 5) Matoba Y. et al. Detection of the Human

Coronavirus 229E, HKU1, NL63, and OC43 between 2010 and 2013 in Yamagata, Japan, Jpn. I. Infect. Dis., 68,138-141(2015)

6) Vabret A. et al. Direct diagnosis of human respiratory coronaviruses 229E and OC43 by the polymerase chain reaction, J. Virological Methods, 97 ,59-66 (2001)

7) Vabret A. et al. Human Coronavirus NL63, France, Emer. Infect. Dis., 11, 1225-1229(2005)

8) Guant E.R. et al. Epidemiology and clinical presentations of the four human coronaviruses 229E, HKU1, NL63, and OC43 detected over 3 years using a novel multiplex real-time PCR method, J. Clin. Microbiol., 48, 2940-2947(2010)

9) IASR https://www.niid.g o.jp/niid/images/iasr/archive/2016/virus/infl1116.pdf

10) HCoV-OC43 ,

, 88(5), 708-710(2015) 11) Matoba Y. et al. An outbreak of Human

Coronavirus OC43 during the 2014-2015 influenza Season in Yamagata, Japan, Jpn. I. Infect. Dis., 68, 442-445(2015)

12) Matoba Y. et al. Trends of Human Coronaviruses in Yamagata, Japan in 2015-2016 Focusing on the OC43 Outbreak of June 2016, Jpn. I. Infect. Dis., 71, 167-169(2018)

13) , , 12, 72-75(2013)

14) Kon M. et al. Detection of human coronavirus NL63 and OC43 in children with acute respiratory infections in Niigata, Japan, between 2010 and 2011, Jpn. I. Infect. Dis., 65, 270-272(2012)

15) NL63 ,

, 29(1), 61-66(2017) 16) Epidemiology and clinical characteristics of human

coronaviruses OC43, 229E, NL63, and HKU1: a study of hospitalized children with acute respiratory tract infection in Guangzhou, China, Eur. J. Microbiol. Infect. Dis., 37, 353-369(2018)

2

Study of Simultaneous Analysis Method for Fusarium Toxin (2nd Report)

Yasuyuki SAKAI, Ikue KONISHI

1,2)

3,4)

LC-MS/MS

LC-MS/MSMRM

5) LC-MS/MS

3-15-

-X HT-2 T-2

DON NIV 3-AcDON 15-AcDON FUXHT-2 T-2 DAS ZEN

Merck

1.0mg10mL 100ppm

1mL10mL

10ppm

VEL ZAN13C-

13C-DON Biopure 25ppm

5.0mg50mL 100ppm

25 L25ppm13C-DON 100 L

250 L 10ppm

LC/MS

Romer MultiSep226MultiSep227 Mycospin400 GLInertSep VRA-1 InertSep VRA-3Autoprep MF-T1500 Merck SupelTox AflaZeaSupelTox Tricho Agilent BondElut Mycotoxin

GL InertSep PLR

Agilent Captiva EMR- Lipid Merck Z-Sep

Prominence 20A/3200Q TRAP /Sciex CAPCELL CORE ADME 2.7 m 2.1mm i.d. 150mm (

A 5mM B5mM

B 10%(0min) 90%(15min) 90%(20min)0.2mL/min 40 5 L MRM

1 LC-MS/MS

100ng/mL

ESI Positive 3-AcDON 15-AcNIV FUX HT-2 T-2 339>231 356>321 372>355 442>263 484>305 339>203 356>137 372>247 442>215 484>185

26,7,8)

ESI Negative

DON NIV VEL 13C-DON

371>281 355>295 325>59 370>310 371>311 355>265 370>279

ESI Positive

DAS VEL ZEN ZAN

384>307 284>267 319>187 321>303 384>229 284>249 319>283 321>189

1 9)

1

2

40 90% 20%90%

80130%

1 10%

3 Negative DON

NIVPositive

100

Positive 100

IQL DONNIV 3-AcDON 15-AcDON FUX ZEN 10ng/mLHT-2 T-2 DAS 1ng/mL

MQLIQL 100

1MultiSep 226 2

MycoSpin400 1mL10,000rpm 1min

0.4mL 10%0.1mL

GL InertSep VRA-1InertSep VRA-3

Romer MultiSep226MultiSep227MycoSpin400 Autoprep MF-T1500

Merck SupelTox AflaZeaSupelTox Tricho

Agilent BondElut Mycotoxin

4 MultiSep 226

MultiSep 227 Autoprep MF-T1500 SupelTox AflaZeaZEN

ZENMultiSep

226MultiSep 226

MultiSep 226

3 MultiSep 226

5

EMR85%

50% 75%

(1) (2)

(3)

6

Negative 13C-DONNIV

DON 13C-DONVEL

Positive ZEN ZANVEL Negative

n=37 30 110%

70 120%Negative DON NIV

Positive

Negative

PositiveVEL ZEN

VEL ZAN

NegativeDON NIV Positive

Negative Positive

VEL ZAN

1) 2015 65. ,562-647,

, (2015) 2) 2015 2.2

,287-305, , (2015) 3)

13

,98-113(2001) 4)

14

,6-9(2002) 5)

,15,72-77(2016) 6) ,53(3),152-156(2012) 7) Yoshinari et al. Journal of Agricultural and Food

Chemistry, 62(5),1174-1180(2014) 8) 20 4 1 19 14729

28 12 22 9) 14 5 21 0521001

157 17

Distribution of Phosphate Ester Flame Retardants in River Water in Fukui.

Takahiro YAMAZAKI, Masanori YOSHIKAWA

HBCD C12H18Br6 26 3

HBCD

9

30 32

200mL

10 L 20mL10mL

OASIS-HLB-Plus20mL

3000rpm 10min8mL

1mLMILLIPORE Millex-LG

LC-MS/MS2 3 4 LC-MS/MS

1)

A B

d-

200ml

N2

8ml

N2 <0.2ml

LC-MS/MS

10ng

183 99225 99267 99

2- 285 632- 327.3 99

327.5 1522- 420 3211,3- -2- 431 99

369 91

198 102246 102294 102

2- 297 672- 345 102

342 822- 433 3301,3- -2- 446 102

390 98

B 10 53 /min 2

4 B30 5 2.8 /min3

n=4 581 118 70

120 %

6 90.20 3.5ng/L

4 30 3229 10 11 30 2 3

85 2

870 ng/L

HBCDHBCD

7,8

HBCD

2- HBCD

HBCD

LC-MS/MS LP-8050CORETECSTM

A 0.1 B 0 5 min A 70 B 30 5 30 min A 70 0 B 30 100 0.2 mL/min 40

[ ]101 11781 116109 11790 9999 11797 11891 10289 10695 108

[ng/L]

0.30 0.950.55 1.80.40 1.4

2- 3.5 122- 0.45 1.5

0.75 2.52- 0.85 2.91,3- -2- 0.90 3.0

0.20 0.75

9

30 32

8

29

1) -LC-MS/MS

,(2017)

HBCD6

9 50 017 5

2- 12 42- 26 6

22 22- 14 51,3- -2- 14 5

12 1

32

HBCD6

22 60 013 5

2- 18 42- 29 5

13 32- 25 51,3- -2- 7 4

21 5

31

2

*1 *2 *3

Development of Basic System Aiming at Stabilization of Waste Landfill

by Pumping Circulation of Leachate (Part 2)

Hirokazu TANAKA, Masamitsu NAKAMURA, Yugo ISOBE* ,

Masatoshi TSUBAKI* , Kazuo KAMURA*3

1-10)

11-15)

2012 2013

16,17)

1

4 m1 m

VP250.5 m

1.55 m 20 L

1.1 m 0.3 MPa 20 L min-1 0.2 MPa

EC (VP25)

2 20 m5 m

107 m 16,17)

6 L min-1

350m 16,18) 90 GL-0.2 m

2009 1.3 m3.11 10-3 cm s-1

*1

*2

*3

2016 3 16 2017 1 312016 8 17 9 9 10 27

11 4

24

1 5 1 2016 10 26 2

- 1 m

3

2.0 m3 6.3 L 16.9 L 71

62 m3 16,17)

3.2 % 10.7 %

15 m4

GL 2 m

1.2 m 1 m

24

EC 5

8 8 17 9 99

12

EC

EC 12

19,20)

EC16,17)

62014

Line-A (2016)4 m

2014 Line-B (2016)4 m 2014

EC5 EC

Line-A (2016)2014

Line-C (2016)

Line-A (2016) 24 m

Line-C (2016) 1 3 mLine-A (2016)

16,17)

EC

ECLine-A Line-C

1

EC

1

1) , , :

, 15,1255-1257(2004)

2)Kamura Kazuo, Hara Yu, Bulent Inac ,Yamada Masato, Inoue Yuzo, Ono Yusaku: Effectiveness of resistivity monitoring for interpreting temporal changes in landfill properties, Journal of material cycles and waste management,7(2), 66-70(2005) 3) , , :

, 19,753-755(2008)

4) , , , , :

, 2010,77-82(2010)

5)Kamura Kazuo, Okuno Manami, Sakurama Toshinori, Wakabayashi Kyoko, Oyama Kenya, Tanaka Hirokazu : Metal enrichment processes and exploration of enriched zones in landfill sites, Proceedings of ISWA World Congress (ISBN978-3-200-03229-3), Electronic file(2013) 6)Kamura Kazuo, Ito Takahiro, Yokose Ryuji, KurokawaMasahiro. Takeuchi Mutsuo,Tanaka,Hirokazu:Exploration of metal-enriched zones in landfills,Proceedings of IWWG-ARB2015,123-128(2015) 7) , , , , :

, 133 ,1-4(2015)

8) , , , , ,: ,

134 ,195-198(2016) 9) , , :

, ,27(1), 27-38(2016)

10) , , , , ,:

, 69(3),173-183(2016) 11) 21

- , ,, ,36(14),1222-1225(2000)

12) ,,

,58(268),544-550(2005) 13)

-, ,32(11),17-21(2006)

14) vol.6 ,77,86-92(2008)

15) , :, , 20(6),

308-313(2009) 16) , , , :

,,12,80-83(2013)

17) , , , , , ,, , , :

, 24,501-502(2013)

18) -http://suidoubox.net/suiribasic17.html

2014.06.16 19) , , , , , ,

: , 36,291-293(2015)

20) , : ,,13,63-66(2014)