Quick GuideZEISS Primostar 3

ZEISS Primostar 3

Original Manual

Carl Zeiss Microscopy GmbHCarl-Zeiss-Promenade 1007745 JenaGermanyinfo.microscopy.de@zeiss.comwww.zeiss.com/microscopy

Carl Zeiss Suzhou Co., Ltd. Modern Industrial Square 3-B, No.333 XingPu Road SIP 215126 Suzhou China

Document Name: ZEISS Primostar 3 Quick GuideOrder Number: 415501-7012-101Revision: 1Language: en-USEffective from: 09/2020

© 2020 Without the prior written consent of ZEISS, this document or any part of it must neither be translated nor reproduced ortransmitted in any form or by any means - including electronic or mechanic methods, by photocopying, recording or by any in-formation or filing system. The right to make backup-copies for archiving purposes shall remain unaffected thereby. Any viola-tions may be prosecuted as copyright infringements.

The use of general descriptive names, registered names, trademarks, etc. in this document does not imply that such names areexempt from the relevant intellectual property laws and regulations and therefore free for general use. This shall also apply ifthis is not specifically referred to. Software programs shall entirely remain the property of ZEISS. No program or subsequent up-grade thereof may be disclosed to any third party, copied or reproduced in any other form without the prior written consent ofZEISS, even if these copies or reproductions are destined for internal use at the customer's only, the only exception being onesingle back-up copy for archiving purposes.

ZEISS reserves the right to make modifications to this document without notice.

ZEISS Table of Contents

Table of Contents

1 General Information ............................................................................................. 4

1.1 Text Conventions and Link Types.......................................................................... 4

1.2 Explanation on Warnings and Additional Information........................................... 5

1.3 Further Applicable Documents.............................................................................. 5

1.4 Contact ................................................................................................................ 6

2 Safety .................................................................................................................... 7

2.1 Intended Use........................................................................................................ 7

2.2 General Safety Notes ............................................................................................ 72.2.1 Safe Operating Condition......................................................................... 72.2.2 Requirements for Users ............................................................................ 8

2.3 Prevention of Hazards .......................................................................................... 8

2.4 Warning and Information Labels........................................................................... 10

2.5 Safety Device of the Reflected-light Fluorescence Illuminator ................................ 12

3 Product and Functional Description..................................................................... 13

3.1 Microscope Primo Star 3, FIX-KÖHLER Versions .................................................... 14

3.2 Microscope Primo Star 3, FULL-KÖHLER Versions ................................................. 16

3.3 Microscope Primo Star 3, FIX-KÖHLER Version with Camera Tube ........................ 18

3.4 Installation Conditions .......................................................................................... 20

4 Switching On/Off Safely........................................................................................ 22

5 How to Get the First Picture ................................................................................. 23

Quick Guide ZEISS Primostar 3 | en-US | Rev. 1 | 415501-7012-101 3

1 General Information | 1.1 Text Conventions and Link Types ZEISS

1 General InformationThis Instruction Manual pertains to the Primostar 3, hereinafter referred to as the "microscope".This Instruction Manual contains basic steps and safety information that must be observed duringoperation and maintenance. For this reason, these operating instructions must be read by the spe-cialist staff / operator prior to commissioning and must always be available at the place of use ofthe microscope. This Instruction Manual belongs to the microscope and must remain with the mi-croscope or be handed over to the purchaser when sold.

1.1 Text Conventions and Link Types

The following text conventions and link types are used:

Text convention Meaning

Click Start.

Press the STANDBY button.

Press [Enter] on the keyboard.

The names of controls and important infor-mation are shown in bold letters.

Press <Ctrl+Alt+Del> Press several keys on the keyboard simultane-ously.

Select Tools > Goto Control Panel > Air-lock.

Follow a path in the software.

Text input Text to be entered by the user

Programming and Macros Anything typed in literally during program-ming, including, for example, macro codes,keywords, data types, method names, vari-ables, class names, and interface names.

Tab. 1: Text convention

Link type Meaning

See: Text Conventions and Link Types [} 4]. Link to further information for this topic.

https://www.zeiss.com/corporate/int/home.html

Link to a website on the internet.

Tab. 2: Link types

4 Quick Guide ZEISS Primostar 3 | en-US | Rev. 1 | 415501-7012-101

ZEISS 1 General Information | 1.2 Explanation on Warnings and Additional Information

1.2 Explanation on Warnings and Additional Information

DANGER, WARNING, CAUTION, and NOTICE are standard signal words used to determine the lev-els of hazards and risks of personal injury and property damage. Not only the safety instructionsand warnings in the Safety chapter are to be considered but also the safety instructions andwarnings in other chapters. Failure to comply with these instructions and warnings can result inboth personal injury and property damage and involve the loss of any claims for damages.

The following symbols and warnings indicating dangerous situations and hazards are used in thisdocument.

DANGER

Type and source of dangerDANGER indicates an imminently hazardous situation which, if not avoided, will result in deathor serious injury.

WARNING

Type and source of dangerWARNING indicates a potentially hazardous situation which, if not avoided, may result indeath or serious injury.

CAUTION

Type and source of dangerCAUTION indicates a potentially hazardous situation which, if not avoided, may result in minoror moderate injury.

NOTICE

Type and source of dangerNOTICE indicates a potentially harmful situation which, if not avoided, may result in propertydamage.

InfoProvides additional information or explanations to help users better understand the contentsof this Instruction Manual.

1.3 Further Applicable Documents

Please take also note of the following documents:

InstallationRequirements

For more details on technical data, refer to the corresponding Installation Requirements.

System and 3rdParty Components,

Accessories

The Microscope can be configured in many ways. Information about the individual components,enhancements and accessories can be obtained from your ZEISS Sales & Service Partner. Also referto the 3rd Party documentation of the manufacturer.

Software For more detailed information on how to use ZEN, please refer to its Online Help.

Safety data sheets Observe the enclosed safety data sheets. The instructions and guidelines given in the respectivesafety data sheets must be complied with.

Downloads Brochures, Safety data sheets, ISO certificates, CSA certificates and EU declarations of conformityas well as software and firmware updates can be found at https://www.zeiss.com/microscopy/int/downloads.html or ask your ZEISS Sales & Service Partner.

Quick Guide ZEISS Primostar 3 | en-US | Rev. 1 | 415501-7012-101 5

1 General Information | 1.4 Contact ZEISS

1.4 Contact

If you have any questions or problems, please contact your local ZEISS Sales & Service Partner orone of the following addresses:

Headquarter

Phone: +49 1803 33 63 34

Fax: +49 3641 64 3439

Email: info.microscopy.de@zeiss.com

Service Germany

Phone: +49 7364 20 3800

Fax: +49 7364 20 3226

Email: service.microscopy.de@zeiss.com

Courses and training

Email: courses.microscopy.de@zeiss.com

ZEISS Sales & Service Partner

You can find a ZEISS Sales & Service Partner in your area under https://www.zeiss.de/mikroskopie/website/forms/sales-and-service-contacts.html.

6 Quick Guide ZEISS Primostar 3 | en-US | Rev. 1 | 415501-7012-101

ZEISS 2 Safety | 2.1 Intended Use

2 SafetyThis chapter contains general requirements for safe working practices. Any person using the Mi-croscope or commissioned with installation or maintenance must read and observe these generalsafety instructions. Knowledge on basic safety instructions and requirements is a precondition forsafe and fault-free operation. Operational safety of the supplied Microscope is only ensured if it isoperated according to its intended use.

If any work is associated with residual risks, this is mentioned in the relevant parts of this docu-ment in a specific note. When components must be handled with special caution, they aremarked with a warning label. These warnings must always be observed.

2.1 Intended Use

The Primostar 3 microscope is an all-purpose, light-optical device used primarily for examining celland tissue cultures. Typical applications are examinations of blood and tissue samples taken fromthe human body or of plant and animal origin.

The microscope, including its original accessories, must not be used for microscopic techniquesother than those described in this Instruction Manual. The manufacturer does not assume any lia-bility for other applications, including those of individual modules or components.

2.2 General Safety Notes

This Instruction Manual must be read before commissioning to ensure safe and permanent opera-tion. Pay particular attention to all listed safety notes. Please ensure that

§ the operating personnel have read, understood and complied with this Instruction Manual, re-lated documents and especially all safety regulations and instructions.

§ the local and national safety and accident prevention regulations are observed.

§ this Instruction Manual is always kept to hand near the microscope.

§ the microscope is in perfect condition.

§ no reflective objects are held in the beam path.

§ the microscope is secured against unauthorized access when not in use.

§ maintenance and repair work, remodeling, removal or replacement of components, as well asany other intervention in the device not described in this document, may only be carried outby the manufacturer ZEISS or persons expressly authorized by ZEISS to do so.

Please observe the generally applicable local and national safety and accident prevention regula-tions, as well as the applicable laws and regulations in your country.

2.2.1 Safe Operating Condition

If it is noted that protection measures are no longer effective or other circumstances occur whichimpair safety and cause changes in operating behavior, the microscope should be shut down im-mediately and a ZEISS service technician should be notified. Make sure that all the protective pan-els and warning labels are attached and readable.

Quick Guide ZEISS Primostar 3 | en-US | Rev. 1 | 415501-7012-101 7

2 Safety | 2.3 Prevention of Hazards ZEISS

2.2.2 Requirements for Users

The microscope, its system components and accessories may only be operated and maintained byauthorized and trained personnel. The user must be aware of the possible dangers involved withmicroscopy and the particular application concerned.

Training As part of the initial commissioning, authorized ZEISS personnel provide basic training on operat-ing the system, which also includes information on device safety and maintenance steps that canbe carried out by the user himself, and a safety briefing. The training provided must be docu-mented accordingly. Special application training is offered for a fee. For training courses on usingthe microscope, visit https://www.zeiss.com/microscopy/int/service-support/training-and-educa-tion.html.

2.3 Prevention of Hazards

This section summarizes potential hazards and recommended safety precautions. Failure to followthe safety instructions and general instructions may result in personal injury and property damage.

Explosion hazard Do not operate the microscope and its accessories in potentially explosive areas, in the presenceof volatile anesthetics or in the presence of combustible solvents, such as alcohol, benzene or sim-ilar chemicals.

Optical RadiationHazard

Gas discharge lights, LED lights and other sources of white light emit strong optical radiation (e.g.UV, VIS, IR). Optical radiation may cause damage to the skin and eyes. The extent of the damagedepends on the parameters wavelength, exposure time and mode of operation (continuous orpulsed).

§ Avoid radiation exposure of eyes and skin.

§ Do not introduce reflective objects into the beam path.

§ Never remove covers or cover panels during operation.

§ Do not disable any interlock system elements.

§ Use suitable protective equipment / protective clothing if required.

Heavy weight The toppling and falling of the microscope may cause injuries to the user. Only operate the deviceon a stable work table with a solid and smooth tabletop.

Risk of burns The microscope emits heat during operation and up to ten minutes after it is switched off. Beforeany transportation, switch off the device and let it cool down. Only operate the device on a stablework table with a solid, non-combustible tabletop.

Damage by dirt orhumidity

Dirt, dust and humidity may impair the performance of the microscope or damage the microscopeor its components. Protect the device against such influences to the greatest possible extent.When not using the microscope, switch it off, let it cool down and cover it with the respectivedust cover.

Voltage hazard Risk of electric shock in case of contact with live parts.

Detachable mains supply must not be replaced with inadequately rated cords. Always use thepower cords supplied by ZEISS. When an unsuitable power cord is used, ZEISS can no longer guar-antee the electrical safety and functionality of the Microscope.

Shut down the Microscope and disconnect the power supply before cleaning.

If an external DC power is connected, it shall be a limited power source.

Don't connect the mains socket and the 12 V DC port as the power input at the same time.

Hazardousspecimen

The microscope is not equipped with special user protection against substances that are corrosive,potentially infectious, toxic, radioactive or otherwise hazardous to health. When handling suchsubstances, make sure to observe all legal regulations, particularly the relevant national accidentprevention regulations.

Immersion oil Immersion oil irritates the skin and the eyes. When using immersion oil, always read the relevantsafety data sheet first. Avoid any contact of the oil with skin, eyes and clothing. After skin con-tact, wash the oil off with plenty of water and soap. After eye contact, immediately rinse the eyewith plenty of water for at least five minutes. If the irritation persists, consult a medical specialist.Ensure that no immersion oil enters the surface water or the sewage system.

8 Quick Guide ZEISS Primostar 3 | en-US | Rev. 1 | 415501-7012-101

ZEISS 2 Safety | 2.3 Prevention of Hazards

False diagnosis The visualized images of the camera 8MPx may only be used for training and research. Direct gen-eration of diagnostic results from these images is not recommended.

Inappropriatetransport

Inappropriate transportation may damage the microscope or cause further material damage. Be-fore any transportation, switch off the device and let it cool down. Only use the original packag-ing for storage or transportation. Maintain the transportation and storage temperatures as speci-fied in Technical Data and Conformity.

Sensitive optics The microscope contains sensitive optical components. Do not touch optical surfaces. Avoid fin-gerprints on the surfaces of lenses and light sources.

Unauthorizedmanipulation

Using components not certified by the microscope manufacturer and any unauthorized openingof the device or its components lead to the loss of all warranty claims. Only use microscope com-ponents supplied by ZEISS. Do not open the device unless you are trained and explicitly authorizedby ZEISS. Always contact ZEISS or a certified service agency for the repair of the instrument.

Inappropriatedisposal

The microscope is not to be disposed of with household waste. Dispose of the device, illuminationbulbs, batteries, the power supply and other components in compliance with the provisions of thelaw in force.

Quick Guide ZEISS Primostar 3 | en-US | Rev. 1 | 415501-7012-101 9

2 Safety | 2.4 Warning and Information Labels ZEISS

2.4 Warning and Information Labels

All points that may pose special risks are additionally marked by warning labels (pictograms) onthe microscope. These warning labels indicate possible dangers. They are part of this instructionmanual. They are to be kept in a clean and legible state. Warning labels that are damaged or nolonger clearly legible must be replaced immediately. Always observe all warning labels on the mi-croscope.

1

2

3

Fig. 1: Positions of labels on the microscope rear side

4

Fig. 2: Position of the label

6

5

Fig. 3: Positions of labels on the fluorescence unit

10 Quick Guide ZEISS Primostar 3 | en-US | Rev. 1 | 415501-7012-101

ZEISS 2 Safety | 2.4 Warning and Information Labels

8

9

7

Fig. 4: Positions of labels on the battery supply unit

The meaning of each warning and information label is explained below.

Posi-tion

Symbol Description

1 Hot surface belowOperate microscope only on a stable, solid,smooth and not tinderlike (non-flammable)surface.

2 Microscope type label

3 Microscope type label

4 Primostar 3EU ImporterCarl Zeiss Microscopy GmbHCarl-Zeiss-Promenade 1007745 Jena, Germany

Quick Guide ZEISS Primostar 3 | en-US | Rev. 1 | 415501-7012-101 11

2 Safety | 2.5 Safety Device of the Reflected-light Fluorescence Illuminator ZEISS

Posi-tion

Symbol Description

5 CAUTIONLED RADIATIONDo not stare at operating lamp. May beharmful to the eyes.

6 Fluorescence unit type label

7 Adjust battery type switch afterchanging battery type

8 Recharcheable batteries onlyNiMH or NiCd size 'D' 9000mAh max.Do not mix different types / capacities

9 Positions of battery type switchON=NIMHOFF=NiCd

Tab. 3: List of attached warning and information labels

2.5 Safety Device of the Reflected-light Fluorescence Illuminator

To prevent personal injury and/or property damage, the reflected-light fluorescence illuminator isequipped with an interlock function, which causes the built-in LED of the reflected-light illumina-tor to be switched off as soon as the reflected-light illuminator is turned with regard to the standor detached.

Defective and damaged safety devices can lead to injuries and damage. In the event of damage ordefect, the affected parts and the entire microscope must be taken out of operation immediatelyand secured against unintentional use.

To check the microscope safety, please contact your ZEISS sales and service partner and keep theservice protocols for your microscope.

12 Quick Guide ZEISS Primostar 3 | en-US | Rev. 1 | 415501-7012-101

ZEISS 3 Product and Functional Description |

3 Product and Functional DescriptionThe Primostar 3 microscopes are transmitted-light microscopes of compact design with a smallfootprint.

The microscopes provide high resolution, infinity-corrected objectives for all important transmit-ted-light techniques, such as brightfield, darkfield, simple polarization, and phase contrast mi-croscopy.

The microscope is optionally available with a camera port for photo and video documentation. Forspecial camera applications the optional binocular tube 25°/22 is equipped with an integratedsmart 8 MP color camera.

Additional information about the hardware configuration and optional enhancements can be ob-tained from your ZEISS Service Technician / Sales Representative or Application Specialists.

Quick Guide ZEISS Primostar 3 | en-US | Rev. 1 | 415501-7012-101 13

3 Product and Functional Description | 3.1 Microscope Primo Star 3, FIX-KÖHLER Versions ZEISS

3.1 Microscope Primo Star 3, FIX-KÖHLER Versions

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Fig. 5: Controls and functional elements of Primo Star 3 FIX-KÖHLER

No. Description of controls Function

1 Eyepiece, adjustable for compensation of defective vision

2 Binocular section of tube for adjustment of interpupillary distance

3 Nosepiece with four or five positions for changing objectives

4 Mechanical stage with specimen holder left forreckless stage

for positioning and fixing of object carrier(s)

5 Lever for opening or closing the aperture diaphragm

6 Abbe condenser 0.9/1.25 for changing between brightfield, phase contrastand darkfield

14 Quick Guide ZEISS Primostar 3 | en-US | Rev. 1 | 415501-7012-101

ZEISS 3 Product and Functional Description | 3.1 Microscope Primo Star 3, FIX-KÖHLER Versions

No. Description of controls Function

7 Centering screws right/left side for centering the image of the luminous-field di-aphragm in the field of view

8 Clamping screw for fixing condenser

9 Collector illumination for inserting filters or mirror

10 Coarse focusing drive (right hand side) for focusing the microscopic image

11 Fine focusing drive (right hand side) for focusing the microscopic image

12 Coaxial knurled knob for stage adjustment for moving and positioning samples in X direction

13 Light intensity knob for varying the brightness of the microscopic im-age

14 Coaxial knurled knob for stage adjustment for moving and positioning samples in Y direction

15 Light intensity indicator for displaying the set light intensity

16 Knocking knob observation tube for fixing the observation tube and other interme-diate tubes

17 DC 5 V switch for switching between mains power supply /12 V DC port and mobile power bank

18 Main switch for switching the microscope on and off

19 5 V USB port for connecting an external power bank

20 Main socket for connecting the power cable

21 12 V DC port for connecting an external 12 V DC powersource, e.g. the battery unit 415501-1814-000

22 Storage place for phase plate adjusting tool for storing the phase plate adjusting tool

23 Holder for power cable for safe storage of the power cable

24 Carrying handle for carrying the microscope

25 Knurled condensor knob for vertical adjustment of condenser

26 Tension knurled ring for adjusting the smoothness of the coarse focus-ing drive

27 Coarse focusing drive (left hand side) for focusing the microscopic image

28 Fine focusing drive (left hand side) for focusing the microscopic image

Quick Guide ZEISS Primostar 3 | en-US | Rev. 1 | 415501-7012-101 15

3 Product and Functional Description | 3.2 Microscope Primo Star 3, FULL-KÖHLER Versions ZEISS

3.2 Microscope Primo Star 3, FULL-KÖHLER Versions

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Fig. 6: Controls and functional elements of Primo Star 3 FULL-KÖHLER

No. Description of controls Function

1 Eyepiece, adjustable for compensation of defective vision

2 Binocular section of tube for adjustment of interpupillary distance

3 Nosepiece with four or five positions for changing objectives

4 Mechanical stage with specimen holder left forreckless stage

for positioning and fixing of object carrier(s)

5 Rotary knob of condenser modulator disc for changing between brightfield, phase contrastand darkfield

6 Lever for opening or closing the aperture diaphragm

16 Quick Guide ZEISS Primostar 3 | en-US | Rev. 1 | 415501-7012-101

ZEISS 3 Product and Functional Description | 3.2 Microscope Primo Star 3, FULL-KÖHLER Versions

No. Description of controls Function

7 Centering knobs right/left side for centering the image of the luminous-field di-aphragm in the field of view

8 Clamping screw for fixing condenser

9 Collector illumination for inserting filters or mirror

10 Lamp exchange drawer for exchanging the lamp

11 Coarse focusing drive (right hand side) for focusing the microscopic image

12 Fine focusing drive (right hand side) for focusing the microscopic image

13 Coaxial knurled knob for stage adjustment for moving and positioning samples in X direction

14 Coaxial knurled knob for stage adjustment for moving and positioning samples in Y direction

15 Light intensity knob for varying the brightness of the microscopic im-age, for switching on / off the Light manager andthe ECO mode

16 Light intensity indicator for displaying the set light intensity

17 Knocking knob observation tube for fixing the observation tube and other interme-diate tubes

18 Main switch for switching the microscope on and off

19 Main socket for connecting the power cable

20 Storage place for phase plate adjusting tool for storing the phase plate adjusting tool

21 Holder for power cable for safe storage of the power cable

22 Carrying handle for carrying the microscope

23 Knurled condensor knob for vertical adjustment of condenser

24 Tension knurled ring for adjusting the smoothness of the coarse focus-ing drive

25 Coarse focusing drive (left hand side) for focusing the microscopic image

26 Fine focusing drive (left hand side) for focusing the microscopic image

Quick Guide ZEISS Primostar 3 | en-US | Rev. 1 | 415501-7012-101 17

3 Product and Functional Description | 3.3 Microscope Primo Star 3, FIX-KÖHLER Version with CameraTube ZEISS

3.3 Microscope Primo Star 3, FIX-KÖHLER Version with Camera Tube

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Fig. 7: Controls and functional elements of Primo Star 3 FIX-KÖHLER with camera tube

No. Description of controls Function

1 Eyepiece, adjustable for compensation of defective vision

2 Binocular section of tube for adjustment of interpupillary distance

3 Nosepiece with four or five positions for changing objectives

4 Mechanical stage with specimen holder left forreckless stage

for positioning and fixing of object carrier(s)

5 Lever for opening or closing the aperture diaphragm

18 Quick Guide ZEISS Primostar 3 | en-US | Rev. 1 | 415501-7012-101

ZEISS3 Product and Functional Description | 3.3 Microscope Primo Star 3, FIX-KÖHLER Version with Cam-

era Tube

No. Description of controls Function

6 Abbe condenser 0.9/1.25 for changing between brightfield, phase contrastand darkfield

7 Centering screws right/left side for centering the image of the luminous-field di-aphragm in the field of view

8 Clamping screw for fixing condenser

9 Collector illumination with insert for filters of mirror

10 Coarse focusing drive (right hand side) for focusing the microscopic image

11 Fine focusing drive (right hand side) for focusing the microscopic image

12 Coaxial knurled knob for stage adjustment for moving and positioning samples in X direction

13 Light intensity knob for varying the brightness of the microscopic im-age

14 Coaxial knurled knob for stage adjustment for moving and positioning samples in Y direction

15 Light intensity indicator for displaying the set light intensity

16 Knocking knob observation tube for fixing the observation tube and other interme-diate tubes

17 LED indicator shows current camera status

18 RESET button for restating the camera

19 SNAP button for taking a photo or video

20 DC 5 V switch for switching between mains power supply /12 V DC port and mobile power bank

21 Main switch for switching the microscope on and off

22 5 V USB port for mobile power bank for connecting an external power bank

23 Main socket for connecting the power cable

24 12 V DC port for connecting an external 12 V DC powersource, e.g. the battery unit 415501-1814-000

25 Storage place for phase plate adjusting tool for storing the phase plate adjusting tool

26 Holder for power cable for safe storage of the power cable

27 Carrying handle for carrying the microscope

28 Knurled condensor knob for vertical adjustment of condenser

29 Tension knurled ring for adjusting the smoothness of the coarse focus-ing drive

30 Coarse focusing drive (left hand side) for focusing the microscopic image

31 Fine focusing drive (left hand side) for focusing the microscopic image

32 Connections of the camera tube please refer to the Instruction Manual

Quick Guide ZEISS Primostar 3 | en-US | Rev. 1 | 415501-7012-101 19

3 Product and Functional Description | 3.4 Installation Conditions ZEISS

3.4 Installation Conditions

The ambient conditions (humidity, temperature, pressure) specified in the Instruction Manualshould be adhered to.

When documenting fluorescence preparations, do not expose the Microscope to direct sunlight.This helps to minimize the influence of stray light.

For prolonged experimental procedures using the Microscope (e.g. involving incubation or longimage-recording procedures) choose an installation location with minimum vibration.

Vibration stemming e.g. from forced ventilation, floor impact noise or other shocks can have animpact on the supplementary modules of the Microscope, some of which react sensitively. It canalso lead to reduced image quality and experimental errors.

To reduce vibrations at the microscope installation location, ZEISS offers various passive and activedamping options; small, air-damped system table, table top mat TS 150).

Select an installation site which is as temperature-stable as possible for long-term observations.

For reliable long-term observations, the Microscope must first warm up. The temperature equilib-rium is attained within 3-5 hours, depending on the light source, electronics and room tempera-ture control. This time must be planned into critical studies.

The following requirements apply regarding installation of the Microscope:

§ The distance of the Microscope to the wall should be at least 15 cm, in order to ensure suffi-cient air circulation and accessibility of the cabling.

§ The dimensions of Primostar 3 stands are shown in the following figures.

Fig. 8: Dimensions of Primostar 3 FIX KÖHLER

20 Quick Guide ZEISS Primostar 3 | en-US | Rev. 1 | 415501-7012-101

ZEISS 3 Product and Functional Description | 3.4 Installation Conditions

Fig. 9: Dimensions of Primostar 3 FULL KÖHLER

Fig. 10: Dimensions of Primostar 3 FIX KÖHLER with camera tube

Quick Guide ZEISS Primostar 3 | en-US | Rev. 1 | 415501-7012-101 21

4 Switching On/Off Safely ZEISS

4 Switching On/Off SafelyThe Primostar 3 is delivered in a fully assembled condition. It can be set up and calibrated by thecustomer himself or through the ZEISS service.

Procedure 1. Connect the Microscope’s power supply withits power cord to an electrical outlet.

2. Switch the Microscope on using the Mainswitch on the rear side of the stand. Pleasealso refer to the complete Instruction Manualwhen using the Microscope.

3. After use, switch the Microscope off using theMain switch on the rear side of the stand.

22 Quick Guide ZEISS Primostar 3 | en-US | Rev. 1 | 415501-7012-101

ZEISS 5 How to Get the First Picture

5 How to Get the First PictureSetting transmitted-light brightfield according to KÖHLER (FULL-KÖHLER version only)

InfoThe described functions are not applicable for FIX-KÖHLER versions. Refer to the InstructionManual for further information.

A

B

C

D

E

1

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46 5Fig. 11: Microscope adjustment in transmitted light brightfield microscopy

Prerequisite ü The Microscope is started up and switched on.

ü The stop is adjusted to prevent the condenser from lifting out the sample.

Procedure 1. Put a high-contrast sample into the specimen holder of the mechanical stage.

2. Adjust the image brightness using the light intensity control 1 on the microscope stand.

3. On condensers with revolver/modulator disks: turn the knurled ring 4 to position BF =brightfield.

4. Bring the 10x objective into position on the nosepiece 7 .

5. Focus the sample using the focus drive 2 .

6. Close the field diaphragm 5 enough to make it visible in the field of view (even ifblurred A ).

7. Lower the condenser using the knurled knob for vertical adjustment 6 until the edge ofthe field diaphragm appears sharp B .

8. Center the field diaphragm image C with both centering screws 3 on the condensercarrier.

9. Open the field diaphragm enough to make the edge of the diaphragm disappear from thefield of view D .

10. To adjust the aperture (contrast), remove an eyepiece from the tube barrel. Lookingthrough the barrel adjust the aperture with the adjusting lever of the aperture diaphragmto the size of approx. 2/3 -4/5 of the diameter of the objective exit pupil E .In most cases this aperture gives the best contrast at almost full resolution and is thus thebest compromise for the human eye.

11. Replace the eyepiece in the tube barrel.

Quick Guide ZEISS Primostar 3 | en-US | Rev. 1 | 415501-7012-101 23

Carl Zeiss Microscopy GmbHCarl-Zeiss-Promenade 1007745 JenaGermany

phone: +49 1803 33 63 34fax: +49 3641 64 3439

info.microscopy.de@zeiss.comwww.zeiss.com/microscopy

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