Workshop on Basics of Flow Cytometry and its Applications in Biomedical Sciences

5th

- 7th

March, 2020

Jointly Organized by

Department of Biotechnology, Babasaheb Bhimrao Ambedkar University (BBAU), Lucknow

Trust for Education and Training in Cytometry (TETC), Mumbai & Flowcytometry Solutions

(FlowSols), Jaipur, Rajasthan

Venue of the Workshop: Department of Biotechnology, Babasaheb Bhimrao Ambedkar University Lucknow, 226025

Contact Information:

For any query and filling registration from, please visit on www.bbau.ac.in or for any

query e-mail us at bbauflow2020@gmail.com

Contact information: +91 9455036926, +91 8874806886, +91 8604351825 WhatsApp on +91 9458450886

*Complete application form should submit along with the Fee Transaction Receipt at mail:

bbauflow2020@gmail.com or through WhatsApp +91 9458450886

For online registration, visit: https://docs.google.com/forms/d/

e/1FAIpQLSd7DNe70YolQAx2-qAiZtkloZhQA6Rmi1NEnaPu_Sb6cLmivA/viewform

~Lecture and wet lab on~ 1. Basics of Flow Cytometry & its Applica-

tions

2. Inside the Black Box— (KYC) Know your Cytometer

3. Quality control in Flow Cytometry

4. DNA and cell cycle analysis

5. Apoptosis, Autophagy and Proliferation

6. Multicolour Immunophenotyping

7. Data Analysis and Presentation

Mode of Registration fee Payment is ONLINE Mode (NEFT/RTGS/UPI)

Account Name: BBAU FLOW CYTOMETRY 2020

Account No: 2900101015191 IFSC No: CNRB0002900

Patron~

Prof. Sanjay Singh,

Hon’ble Vice Chancellor, BBAU

Chairpersons~

Prof. Dinesh Raj Modi &

Prof. Shubhini A. Saraf, BBAU, Lucknow

Co-Chairperson~

Prof. Sangeeta Saxena, DBT, BBAU

Organizing Secretaries~

Dr. Gosipatala Sunil Babu, BBAU

Dr. Hemant Agrawal, Flowsols, India

Convenor~

Dr. Rekha Gour, TETC, Mumbai

Joint Secretary~

Dr. Monica Sharma, BBAU

Treasurer~

Dr. Yusuf Akthar, BBAU

~:Pedagogue:~

Dr. Khaliqur Rahman, SGPGIMS, Lucknow

Dr. S. K. Singh, KGMC, Lucknow

Dr. Sanjay Yadav, AIIMS, Rae Bareli

Dr. Mrigank Srivastava, CSIR-CDRI, Lucknow

Dr. Hemant Agrawal, Flowcytometry Solutions, Jaipur,

India

Dr. Rekha Gour, Co-founder, TETC, Mumbai.

Dr. Anvesha Mallik, Beckman Coulter, New Delhi

Dr. Subhadeep Mujharjee, BD, Bangalore

Dr. G. Sunil Babu, DBT, BBAU, Lucknow

Advisory Committee~

Prof. D.P. Singh, Director, IQAC, BBAU

Prof. R.P. Singh, Dean, Acad. Affairs, BBAU

Prof. R.B. Ram, Dean , SAST, BBAU

Prof Sunita Mishra, Dean, SHS, BBAU

Prof. S. Victor Babu, Registrar, BBAU

Prof. S.K. Dwivedi, Dean, SES, BBAU

Prof. Kaman Singh, Head, DOAC, BBAU

Prof. B.S. Bhaduria, Dean, DSW

Prof Ram Chandra, DD, IQAC, BBAU

Prof. B.B. Malik, Proctor, BBAU

Prof. Kamal Jaiswal, Head, DOZ, BBAU

Prof. Sanjay Kumar, Head, DOH, BBAU

Prof. N.K. Arora Head, DES, BBAU

Prof Rajesh Kumar, Head, DEM, BBAU

Prof. B.C. Yadav, DOAP, BBAU

Prof. Gajanan Pandey, PIC, Security, BBAU

Mr. Ashish Rastogi, Finance Officer, BBAU

Dr. U.V. Kiran, Head, HD& FS, BBAU

Delegates Early Bird Registration On Spot Registration

Students Rs. 1000 Rs. 1500

Research Scholar/ RA/PDFs Rs. 1500 Rs. 2000

Faculty Rs. 2000 Rs. 2500

Support and Sponsored by

LABLINE TRADERS

Workshop on

Basics of Flow Cytometry and its Applications in

Biomedical Sciences

Babasaheb Bhimrao Ambedkar University (BBAU)

Trust for Education and Training in Cytometry (TETC)

Flowcytometry Solutions (FlowSols)

at

Registration

Last date for registration for the workshop

29th February, 2020

Venue of the Workshop

Department of Biotechnology

Babasaheb Bhimrao Ambedkar University

Vidya Vihar, Rae Bareli Road, Lucknow,

Uttar Pradesh– 226025 (India)

Contact Information:

For any query, please visit on www.bbau.ac.in or

e-mail us at bbauflow2020@gmail.com

For query, feel free to contact +91 9455036926,

+91 8874806886, +91 8604351825

WhatsApp on +91 9458450886

Account Details

Account No: 2900101015191

(BBAU FLOW CYTOMETRY 2020)

Bank Name: Canara Bank

Branch: BBA University Branch, Lucknow

IFSC Code: CNRB0002900

• Registration includes workshop kit and working Lunch Coupons

• Accommodation will be provided on first come and first serve basis. Charges of accommodation will be extra.

Patron

Prof. Sanjay Singh,

Hon’ble Vice Chancellor,

BBAU, Lucknow

Chairpersons

Prof. Dinesh Raj Modi,

Dept. of Biotechnology, BBAU, Lucknow

Prof. Shubhini A. Saraf,

Dept. of Pharmaceutical Sciences, BBAU, Lucknow

Co-Chairperson

Prof. Sangeeta Saxena,

Dept. of Biotechnology, BBAU, Lucknow

Organizing Secretaries

Dr. Gosipatala Sunil Babu

Dept. of Biotechnology, BBAU, Lucknow

Dr. Hemant Agrawal,

Flowcytometry Solutions, Jaipur

Convenor

Dr. Rekha Gour,

Trust for Education & Training in Cytometry, Mumbai

Joint Secretary

Dr. Monica Sharma,

Dept. of Biotechnology, BBAU, Lucknow

Treasurer

Dr. Yusuf Akthar,

Dept. of Biotechnology, BBAU, Lucknow

Department of Biotechnology,

School of Life Sciences

Babasaheb Bhimrao Ambedkar University

Lucknow-226025, INDIA

For updates, please follow us on Facebook https://www.facebook.com/tetcindia/

5th to 7th March, 2020

Jointly Organized by

Delegates Early Bird Registration

On Spot Registration

Students 1000 1500

Research Scholar/ RA/PDFs

1500 2000

Faculty 2000 2500

Instrumentation Support by

Sponsored by:

LABLINE

TRADERS

About University:

Babasaheb Bhimrao Ambedkar University is a Central University established on 10th January, 1996 by the act of Parliament 1994, with a objects of promoting advance knowledge by in structural and re-search facilities in key and frontier areas of Science & Technolo-gy and several others disciplines. BBAU is a residential Universi-ty having spread over 250 acres of land at Raebareli Road, dis-tance about 11 Kms from the Charbagh Railway station and 5kms approx. from the Chaudhary Char an Singh International Airport, Lucknow. One of the chief objective of the University is to cater to, and promote the educational needs of the marginal-ized people, particularly the SC/ST communities and Women.

The University is progressing continuously towards becoming an center of Excellence, both in academics as well as in infra-structural and is already endowed with various facilities viz innovation Club, University Science Instrumentation Centre, State of Art Library, National Knowledge Network, Remedial Coaching Centre for SC/ST students, Information & Guidance bureau, health Centre etc. The University has good facility and laboratories in different departments of the University to em-phasized the research for human welfare and mankind.

About the Workshop

The theme of the workshop is “Basics of Flow Cytometry and its

Applications in Biomedical Sciences”. Today, flow cytometry

has become an important tool to unravel the mysteries in

the cells. The applications of flow cytometry have spread

through all branches of biological sciences especially in bio-

technology involved in exploration of Stem cells, genomics

and proteomics. Flow cytometry has been used to measure

the properties of both mammalian and plant cells as well

yeast and bacteria. This technique can also be used to study

for various purpose like for isolated nuclei , chromosome

and mitochondria. Flow cytometer is an equipment which

helps to measure certain physical and chemical characteristics of

the cells or particle as they travel one by one through a sensing

point. The key feature of Flow Cytometric analysis is that sepa-

rate measurements are made on each individual cell particle

with in the suspension rather than an average value of the whole

population. In this process, the cells that have been fluoro-

chrome labeled antibody in suspension are introduced into a

liquid jet and passed individually through the laser beam.

As each cells passes through, it emits a fluorescence and scattered

light, these signals are collected and converted by the flow cytofluo-

rometer to give quantitative information on the intensity of fluores-

cence and the light scattering properties of each cell. The ability of

flow cytometer to measure multiple cellular parameters, based on

light scattered and fluorescence to purify physically sub population

of cells, has lead to growing utilization of this technique in biology

and medicine. In cells, a variety of properties can be measured viz

the DNA content of the nucleus, expression of a surface or intra-

cellular antigen, activity of an intracellular enzyme, functional

studies of chromosomes for creating gene libraries. Apart from

above, this technique can be used for direct cloning, single or

rare transfected or hybridoma cells into each tissue culture plate

can also be studied.

This workshop will cover the following broad topics:

1. Basics of Flow Cytometry & its Biomedical Applications

2. Inside the Black Box— (KYC) Know your Cytometer

3. Quality control in Flow Cytometry

4. DNA and cell cycle analysis

5. Apoptosis, Autophagy and Proliferation

6. Multicolour Immunophenotyping

7. Data Analysis and Presentation

This workshop on application of cytometry in biomedical sciences will immensely help the BBAU fraternity and the delegates by providing a hands on training for 3 days by the eminent faculty from esteemed Institutes.

Dr. Hemant Agrawal

Flow Cytometry Solutions

Dr. Rekha Gour

TETC, India

Dr. G. Sunil Babu

BBAU, Lucknow

Dr. K Rahmaan

SGPGI, Lucknow

Dr. Sanjay Yadav

AIIMS, Rae Bareli

Dr. Mrigank Srivastava

CSIR-CDRI, Lucknow

5th to 7th March, 2020

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Note: Please fill this Registration Form completely and mail it to flowbbau2020@gmail.com or WhatsApp on +91 9458450886 Dr. S K Singh

KGMU, Lucknow

Workshop on Basics of Flow Cytometry and its

Applications in Biomedical Sciences