Electron Microscopy - Scanning electron microscope, Transmission Electron Microscope
What type of microscope are we using? Microscope Types CompoundDissection Scanning Electron...
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Transcript of What type of microscope are we using? Microscope Types CompoundDissection Scanning Electron...
![Page 1: What type of microscope are we using? Microscope Types CompoundDissection Scanning Electron Microscope (SEM) Transmission Electron Microscope (TEM) Compound.](https://reader036.fdocuments.in/reader036/viewer/2022081506/56649e5c5503460f94b53de0/html5/thumbnails/1.jpg)
![Page 2: What type of microscope are we using? Microscope Types CompoundDissection Scanning Electron Microscope (SEM) Transmission Electron Microscope (TEM) Compound.](https://reader036.fdocuments.in/reader036/viewer/2022081506/56649e5c5503460f94b53de0/html5/thumbnails/2.jpg)
What type of microscope are we using?
Microscope Types
Compound Dissection
Scanning Electron
Microscope (SEM)
Transmission Electron
Microscope (TEM)
Compound
![Page 3: What type of microscope are we using? Microscope Types CompoundDissection Scanning Electron Microscope (SEM) Transmission Electron Microscope (TEM) Compound.](https://reader036.fdocuments.in/reader036/viewer/2022081506/56649e5c5503460f94b53de0/html5/thumbnails/3.jpg)
Compound Microscope
A compound microscope consists of an objective lens and an eyepiece at opposite ends of an adjustable tube.
![Page 4: What type of microscope are we using? Microscope Types CompoundDissection Scanning Electron Microscope (SEM) Transmission Electron Microscope (TEM) Compound.](https://reader036.fdocuments.in/reader036/viewer/2022081506/56649e5c5503460f94b53de0/html5/thumbnails/4.jpg)
arm
base
stage
course adjustment knob
nosepiece
scanning objective lens
diaphragm
light source
stage clips
high-power objective lens
low-power objective lens
fine adjustment knob
eyepiece
![Page 5: What type of microscope are we using? Microscope Types CompoundDissection Scanning Electron Microscope (SEM) Transmission Electron Microscope (TEM) Compound.](https://reader036.fdocuments.in/reader036/viewer/2022081506/56649e5c5503460f94b53de0/html5/thumbnails/5.jpg)
Arm and Base
These two pieces are used to tilt, lift, support and carry the microscope carefully.
![Page 6: What type of microscope are we using? Microscope Types CompoundDissection Scanning Electron Microscope (SEM) Transmission Electron Microscope (TEM) Compound.](https://reader036.fdocuments.in/reader036/viewer/2022081506/56649e5c5503460f94b53de0/html5/thumbnails/6.jpg)
Light Source
This part provides light to view the specimen.
![Page 7: What type of microscope are we using? Microscope Types CompoundDissection Scanning Electron Microscope (SEM) Transmission Electron Microscope (TEM) Compound.](https://reader036.fdocuments.in/reader036/viewer/2022081506/56649e5c5503460f94b53de0/html5/thumbnails/7.jpg)
Course Adjustment Knob
This knob moves the body tube or stage (depending on your microscope) up and down.
![Page 8: What type of microscope are we using? Microscope Types CompoundDissection Scanning Electron Microscope (SEM) Transmission Electron Microscope (TEM) Compound.](https://reader036.fdocuments.in/reader036/viewer/2022081506/56649e5c5503460f94b53de0/html5/thumbnails/8.jpg)
Stage
This is the area to place your specimen for viewing.
![Page 9: What type of microscope are we using? Microscope Types CompoundDissection Scanning Electron Microscope (SEM) Transmission Electron Microscope (TEM) Compound.](https://reader036.fdocuments.in/reader036/viewer/2022081506/56649e5c5503460f94b53de0/html5/thumbnails/9.jpg)
Diaphragm
This part of the microscope regulates the amount of light that enters through the stage opening.
![Page 10: What type of microscope are we using? Microscope Types CompoundDissection Scanning Electron Microscope (SEM) Transmission Electron Microscope (TEM) Compound.](https://reader036.fdocuments.in/reader036/viewer/2022081506/56649e5c5503460f94b53de0/html5/thumbnails/10.jpg)
Stage Clips
These hold the slide in position.
![Page 11: What type of microscope are we using? Microscope Types CompoundDissection Scanning Electron Microscope (SEM) Transmission Electron Microscope (TEM) Compound.](https://reader036.fdocuments.in/reader036/viewer/2022081506/56649e5c5503460f94b53de0/html5/thumbnails/11.jpg)
Nosepiece
This piece contains all of the objective lenses.
![Page 12: What type of microscope are we using? Microscope Types CompoundDissection Scanning Electron Microscope (SEM) Transmission Electron Microscope (TEM) Compound.](https://reader036.fdocuments.in/reader036/viewer/2022081506/56649e5c5503460f94b53de0/html5/thumbnails/12.jpg)
Fine Adjustment Knob
This is a knob that helps to focus on fine details of the specimen.
![Page 13: What type of microscope are we using? Microscope Types CompoundDissection Scanning Electron Microscope (SEM) Transmission Electron Microscope (TEM) Compound.](https://reader036.fdocuments.in/reader036/viewer/2022081506/56649e5c5503460f94b53de0/html5/thumbnails/13.jpg)
Eyepiece
This is the piece that you look through to see the specimen.
![Page 14: What type of microscope are we using? Microscope Types CompoundDissection Scanning Electron Microscope (SEM) Transmission Electron Microscope (TEM) Compound.](https://reader036.fdocuments.in/reader036/viewer/2022081506/56649e5c5503460f94b53de0/html5/thumbnails/14.jpg)
Objective Lenses
These pieces enlarge the specimen to scanning, low, and high level of magnification.
![Page 15: What type of microscope are we using? Microscope Types CompoundDissection Scanning Electron Microscope (SEM) Transmission Electron Microscope (TEM) Compound.](https://reader036.fdocuments.in/reader036/viewer/2022081506/56649e5c5503460f94b53de0/html5/thumbnails/15.jpg)
Now we know all of the parts, but how do we use
the microscope?
![Page 16: What type of microscope are we using? Microscope Types CompoundDissection Scanning Electron Microscope (SEM) Transmission Electron Microscope (TEM) Compound.](https://reader036.fdocuments.in/reader036/viewer/2022081506/56649e5c5503460f94b53de0/html5/thumbnails/16.jpg)
Step 1
• To carry the microscope grasp the microscopes arm with one hand.
• Place your other hand under the base. Place the microscope on a table with the arm toward you.
• Plug the microscope in and turn on the light source or adjust the mirror. Make sure that the cords do not obstruct walking paths.
![Page 17: What type of microscope are we using? Microscope Types CompoundDissection Scanning Electron Microscope (SEM) Transmission Electron Microscope (TEM) Compound.](https://reader036.fdocuments.in/reader036/viewer/2022081506/56649e5c5503460f94b53de0/html5/thumbnails/17.jpg)
Step 2
• Turn the coarse adjustment knob to lower the stage.
![Page 18: What type of microscope are we using? Microscope Types CompoundDissection Scanning Electron Microscope (SEM) Transmission Electron Microscope (TEM) Compound.](https://reader036.fdocuments.in/reader036/viewer/2022081506/56649e5c5503460f94b53de0/html5/thumbnails/18.jpg)
Step 3
• Revolve the nosepiece until the scanning objective lens clicks into place.
![Page 19: What type of microscope are we using? Microscope Types CompoundDissection Scanning Electron Microscope (SEM) Transmission Electron Microscope (TEM) Compound.](https://reader036.fdocuments.in/reader036/viewer/2022081506/56649e5c5503460f94b53de0/html5/thumbnails/19.jpg)
Step 4
• Adjust the diaphragm so that it lets in the most light possible.
• Look through the eyepiece to assure that all parts are correctly aligned.
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Step 5
• Place a slide on the stage. Center the specimen over the opening on the stage. Use the stage clips to hold the slide in place.
![Page 21: What type of microscope are we using? Microscope Types CompoundDissection Scanning Electron Microscope (SEM) Transmission Electron Microscope (TEM) Compound.](https://reader036.fdocuments.in/reader036/viewer/2022081506/56649e5c5503460f94b53de0/html5/thumbnails/21.jpg)
Step 6
• Look at the stage from the side. Carefully turn the coarse adjustment knob to raise the stage until the scanning power objective almost touches the slide.
![Page 22: What type of microscope are we using? Microscope Types CompoundDissection Scanning Electron Microscope (SEM) Transmission Electron Microscope (TEM) Compound.](https://reader036.fdocuments.in/reader036/viewer/2022081506/56649e5c5503460f94b53de0/html5/thumbnails/22.jpg)
Step 7
• Looking through the eyepiece, VERY SLOWLY turn the coarse adjustment knob to lower the specimen until it comes into focus.
![Page 23: What type of microscope are we using? Microscope Types CompoundDissection Scanning Electron Microscope (SEM) Transmission Electron Microscope (TEM) Compound.](https://reader036.fdocuments.in/reader036/viewer/2022081506/56649e5c5503460f94b53de0/html5/thumbnails/23.jpg)
Step 8
• To switch to the high power objective lens, look at the microscope from the side. CAREFULLY rotate the nosepiece until the high-power objective lens clicks into place. Make sure the lens does not hit the slide.
![Page 24: What type of microscope are we using? Microscope Types CompoundDissection Scanning Electron Microscope (SEM) Transmission Electron Microscope (TEM) Compound.](https://reader036.fdocuments.in/reader036/viewer/2022081506/56649e5c5503460f94b53de0/html5/thumbnails/24.jpg)
Step 9
• Now switch to the low power objective lens, look at the microscope from the side. CAREFULLY rotate the nosepiece until the low-power objective lens clicks into place. Make sure the lens does not hit the slide.
![Page 25: What type of microscope are we using? Microscope Types CompoundDissection Scanning Electron Microscope (SEM) Transmission Electron Microscope (TEM) Compound.](https://reader036.fdocuments.in/reader036/viewer/2022081506/56649e5c5503460f94b53de0/html5/thumbnails/25.jpg)
Step 10
• Looking through the eyepiece, turn the fine adjustment knob until the specimen comes into focus.
![Page 26: What type of microscope are we using? Microscope Types CompoundDissection Scanning Electron Microscope (SEM) Transmission Electron Microscope (TEM) Compound.](https://reader036.fdocuments.in/reader036/viewer/2022081506/56649e5c5503460f94b53de0/html5/thumbnails/26.jpg)
Step 11
• Diagram your observation through each of the objective lenses.
Wow…this is huge!
![Page 27: What type of microscope are we using? Microscope Types CompoundDissection Scanning Electron Microscope (SEM) Transmission Electron Microscope (TEM) Compound.](https://reader036.fdocuments.in/reader036/viewer/2022081506/56649e5c5503460f94b53de0/html5/thumbnails/27.jpg)
You are now a…