UNIVERSITY OF HEALTH SCIENCES LAHORE, PAKISTAN · UNIVERSITY OF HEALTH SCIENCES LAHORE, PAKISTAN ....

1

CURRICULUM

FOR

4 YEARS DEGREE PROGRAMME

IN

MEDICAL LABORATORY TECHNOLOGY

B.Sc (HONS.)

2007

UNIVERSITY OF HEALTH SCIENCES

LAHORE, PAKISTAN

2

GOVERNOR'S HOUSE

LAHORE

FOREWORD

1. The establishment of University of Health Sciences is a milestone in the efforts of the

Government to ensure quality medical education in the province. The Government of

Punjab has achieved tremendous progress in providing quality education with the

establishment of this University.

2. With a view to bring about further improvement in medical education, the

undergraduate programmes are being reviewed on a continuous basis so as to improve

their quality and to bring them at par with international standards.

3. The Government has focused on other components relating to health sciences and has

particularly been successful in bringing about a significant improvement in the field of

pharmacy. In addition, the Government of Punjab has undertaken a new initiative by

introducing a four years degree programme in Allied Health Sciences. Considering the

rapid technical developments in the health systems around the world, the Allied Health

Sciences programme is a step forward to fill up the gap in the prevailing health systems

in the province.

4. I am happy to point out that all medical colleges affiliated with the University of

Health Sciences have unanimously been able to put up a comprehensive programme

covering 17 disciplines in the Allied Health Sciences. This indeed is a very positive,

appreciative and commendable effort.

5. I wish them success in their endeavors.

LT. GEN. (R) KHALID MAQBOOL

H.I., H.I.(M)

UNIVERSITY OF HEALTH SCIENCES LAHORE

VICE

CHANCELLOR

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PREFACE

A university is the zenith of knowledge that imparts quality education and awards degrees for

extensive educational attainments in various disciplines. Protection of traditional knowledge, making

exploration about it and obtaining deep understanding about modern technology and research

techniques are some of responsibilities of the university. The mission of University of Health Sciences

Lahore (UHS) is chartered to develop an intellectually conducive environment providing excellence

and innovation in medical education and research to produce competent and community oriented

doctors, dentists, nurses, bio-medical engineers, and paramedics.

Allied Health Sciences is a field in medicine which has been completely neglected up till now so that

there is absolute dearth of trained Allied Health personnel who are the actual service providers to the

patients. As a matter of fact they form the connecting link between the doctors and the patients which

is missing altogether in our health care system.

Realizing all this UHS Lahore for the first time took the lead to organize education and training at

B.Sc Honors level in all disciplines of Allied Health Sciences. Fifteen disciplines have been identified

in which these programmes are launched in all the UHS affiliated medical institutions in both public

and private sectors. These programmes include; Medical Laboratory Technology, Medical Imaging

Technology, Emergency & Intensive Care, Physiotherapy, Optometry and Orthoptics, Orthotic and

Prosthetic Sciences, Respiratory Therapy, Cardiac Perfusion, Dental Technology, Occupational

Therapy, Speech & Language Pathology , Operation Theater Technology, Nutrition, Audiology and

Biomedical Engineering. This is going to be a remarkable development in the field of medicine in our

country and will fill up the existing gaps in the health delivery system

All this has been achieved with constant support and guidance of Honorable Chancellor / Governor of

the Punjab Lt. Gen. (Retd.) Khalid Maqbool who has actually played a pivotal role in organizing these

programmes. Government of the Punjab in the Health Department is fully committed to support this

educational programme and has instructed all the medical institutions under its administrative control

to launch these programmes in their respective institutions.

This document precisely briefs the details of B.Sc Programme in Medical Lab Technology as

prepared by the experts’ committee. I am pleased to acknowledge the efforts made by Dr. I. A.

Naveed, Dr. Asad Zaheer, Dr. Asim Mumtaz, Dr. Obaidullah Shakir (CH & ICH), Dr. Nadia Naseem

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and the members of committee consisting of Professor A. H. Nagi (LMDC), Professor Nausheen

Waseem Yousaf (AIMC) and Prof. M. Sarwar Bhatti (LMDC). The contribution made by them will go

a long way in the education and training in the field of AHS.

I hope this programme will meet the latest trends in Medical Laboratory Technology and will

certainly produce competent paramedical personnel to fill in the gap in the system which is main

objective of this programme.

Prof. M. H. Mubbashar Hilal-e-Imtiaz, Sitara-e-Imtiaz

MB, FRCP, FCPS Psych, FRC Psych, DPM

Vice Chancellor/ Chief Executive

University of Health Sciences, Lahore

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1. Aims and Objectives of the Course 1

2. Specific Learning Outcomes 2

3. Nomenclature and Duration 3

4. Eligibility Criteria for Admission 5

5. Recognition/Equivalence of the Degree and Institution 6

6. Methods of Instruction/Course Conduction 7

7. Quality Assurance In Education 8

8. Skills To Be Learnt During The Course 10

9. Equipments and Gadgets 11

10. Examinations 13

11. Appendix A: Outlines Of Professional Examinations 16

12. Appendix B : Course Contents: First Professional

Examinations

27

13. Course Contents : Second Professional Examinations 46

14. Course Contents : Third Professional Examinations 56

15. Course Contents : Final Professional Examinations 63

16. Recommended Books 65

Sr.

No

CONTENTS

Page

No.

6

AIMS AND OBJECTIVES OF THE COURSE

AIMS:

The aim of the 4 years degree programme in Medical Laboratory Technology is to

equip the students with relevant professional knowledge, skills, techniques and ethical

values to enable them to apply their acquired expertise at level between the doctors and

the patient for efficient health service delivery.

GENERAL LEARNING OBJECTIVES:

Medical Laboratory Technology education and training should enable the student:

To develop accuracy and meticulousness to attain high levels of ethics and technical

proficiency

To assess the technical ad non technical skills in a standardized and

reproducible environment

To strengthen the decision power and exercise appropriate judgment skills, to

be applied especially during crisis.

To enhance both the stamina and the patience levels to bear the burden.

To develop good leadership, problem solving, planning and management skills.

To develop and analyze innovative strategies for effective communication with the

patients.

To develop a sense of motivation for the patients and himself too.

To demonstrate interdisciplinary team building strategies for effective co ordination

between various Allied Health Disciplines.

To demonstrate understanding of the basic concepts of professional behavior

and legal implications of the work environment.

To demonstrate the knowledge of his / her role in health care delivery system.

In establishing and maintaining continuing education as a function of growth

and maintenance of professional competence.

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SPECIFIC LEARNING OUTCOMES

Following competencies will be expected from a student completing 4 years degree

course in Medical Laboratory Technology. The student should be able to:

Learn and apply the practical theory (new techniques and procedures) of the

laboratory directly to workplace settings

Demonstrate knowledge of medical terminology with special emphasis on writing

and understanding the laboratory reports.

Select and use appropriate, safe and effective tools to solve a variety of

problems pertaining to collecting, transporting, handling and conducting tests

on laboratory samples

Demonstrate knowledge of utilizing and performing corrective and preventative

maintenance on a variety of instruments and sensitive (automated) equipments.

Relate laboratory results to common disease process and draw and defend

reasonable conclusions with proper satisfaction of the patients and their

concerns.

Perform and monitor quality control within predetermined limits in the laboratory.

Help in the upgrading and expansion of the instrumentation and equipments and

other supplies used in the laboratory.

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NOMENCLATURE AND DURATION

NOMENCLATURE:

The name of the degree programme shall be B.Sc Medical Laboratory Technology. The

duration of the course shall be 4 years with structured training in a recognized

department under an approved supervisor.

COURSE TITLE:

B.Sc Medical Laboratory Technology

TRAINING CENTERS:

Departments of Pathology accredited by UHS for this training in the affiliated institutes

of the University of Health Sciences, Lahore.

COURSE DURATION:

Four years structured training in a recognized department under the guidance of an

approved supervisor.

COURSE SCHEME:

The training is spread over four years with a specific component for each year of

training.

FIRST YEAR:

Theoretical component:

1.

2.

3. pBasic Anatomy

4. Basic Physiology

5. Basic Biochemistry

6. General Pathology

7. Behavioural Sciences

8. Islamiyat

9. Pakistan studies

10. Computer Education

Practical component:

Hand- on training in basic techniques related to the discipline.

SECOND YEAR:

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1. Haematology and Blood Banking

2. Histopathology and Cytopathology


Hand- on training in laboratory techniques in above mentioned disciplines

THIRD YEAR:


1. Microbiology

2. Chemical Pathology

3. Immunology And Serology


Hand- on rotational training in laboratory techniques in above mentioned disciplines.

FOURTH YEAR:


1. Elective Subjects:

Immunohematology

Advanced Clinical Chemistry

Clinical Microbiology

Advanced Clinical Immunology

Medical Genetics

Molecular Biology

2. Biostatistics And Research Methodology


Advanced lab training related to elective subjects

Research assignments related to elective subjects

Training as Trainers

The students during final year of the programme will be involved actively in the teaching

& training of the junior classes i.e. 1st year & 2nd year so that the seniors become mentors

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for the juniors. These educational activities will be carried out under the direction and

supervision of a faculty member.

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ELIGIBILITY CRITERIA FOR ADMISSION:

DOCUMENTS REQUIRED FOR ADMISSION:

Completed B.Sc Medical Laboratory Technology application form

Copy of the Matriculation Certificate

Copy of the F.Sc / Equivalent Examination Certificate with detailed marks

sheet

Copy of the entry test result card

3 passport size photographs

GENERAL REQUIREMENTS:

Securing pass percentage in the entry test.

Qualifying the interview successfully.

Having up to the marks credentials (No. of attempts, any medal or

distinction).

REGISTRATION AND ENROLLMENT:

Total number of students enrolled must not exceed 10 per department.

UHS will approve supervisors for the course.

Candidates selected will be registered with the approved supervisor and enrolled

with UHS.

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RECOGNITION / EQUIVALENCE OF THE DEGREE AND THE

INSTITUTION

After four years training and education, the candidates on successful completion of the course will

be awarded a graduate degree in Allied Health Sciences by the UHS, equivalent to any other

similar qualifications.

ACCREDITATIONS OF THE TRAINING INSTITUTION:

The relevant department of an affiliated institute will get accreditation for training programme

based on :

Faculty: properly qualified and trained faculty for education of the programme.

Adequate space: including class rooms with the audiovisual aids, clinical laboratory

in all the disciplines of the Pathology, Computer labs.

Library: Departmental library should have latest editions of the recommended books,

reference books and Journals related to the laboratory technology.

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METHODS OF INSTRUCTIONS

As a policy, active participation of the students will be encouraged. Following teaching

modalities will be employed:

Large group teachings (lectures)

Small group teachings

Seminar presentations

Assignments

Skills teachings

Self study and use of internet.

In addition, to promote interactive and communication skills, following methods will be used;

MONTHLY STUDENTS MEETINGS:

Journal club meeting

Core curriculum meeting

Skill development

ANNUAL GRAND MEETING:

Once a year all students enrolled at UHS from different institutes will be invited to annual

meeting at UHS. The students will present their annual reports. Issues and concerns related to

each discipline will be discussed. Feedback should be collected and suggestions should be

sought in order to involve students in decision making. Any research or literary work done by

the students will be displayed.

In the evening an informal dinner and gathering can be arranged. This will help in creating a

sense of belonging and ownership by the students and the faculty.

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QUALITY ASSURANCE IN EDUCATION

Quality assurance in health education is a broad spectrum of plans, policies and procedures that

provides a baseline structure to achieve quality goals. The quality planning, quality improvement

and quality assessment are main areas of quality assurance which need thorough intervention

based on professional concepts. It is sum of the processes of assessing and stimulating the quality

of health education by measuring outcome and comparing it with current criteria and demands of

health care. Quality assurance should ensure that activities of health education are systematic and

controlled. It should affect all levels of the health community and every professional working in

health care.

Guidelines For Regional And International Standards:

Mission and Objectives

Educational Programme

Assessment of Students

Student Education and Support

Academic Staff

Educational Resources

Governance and Administration

Continuous Renewal

This educational programme has been designed and will be carried out strictly based

on the above mentioned guidelines.

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QUALITY ASSURANCE IN LABORATORY MEDICINE

Quality assurance in clinical laboratory includes all actions which a laboratory takes to

ensure quality results. It is a broad spectrum of plan, policies, and procedures which are

adopted by the clinical laboratory to minimize preanalyctical, analytical and postanalytical

errors. The key to effective laboratory quality assurance is that all the procedures, protocols

and actions are performed for the purpose of reporting quality results in assisting the

physician for the maintenance of excellence in patient care. Quality control is the middle

component of quality assurance which comprises of internal and external quality control

programs.

Internal Quality Control:

Internal quality control program is run daily along with patient samples to recognize and

minimize systematic and random errors in analytical process. It is the responsibility of the

laboratory to implement and carry out internal quality control program on regular basis to

deliver accurate and precise tests results.

External Quality Control:

External quality control program is run by international and national agencies to monitor

the performance of laboratories. There are different external quality assurance programs

like CAP (College of American Pathologist), RIQAS (Randox International Quality

Assurance Scheme), EQAS (External Quality Assurance Services by Bio-rad), and

NEQAPP (National External Quality Control Program Pakistan). It is the duty and

obligation of laboratory to enroll with one of the external quality assurance schemes. The

basic operation of these programs involve the participating laboratory to analyze the same

lot of control material on monthly, bi-weekly, or daily basis along with the internal quality

control material. The results are tabulated and sent for the evaluation by the sponsoring

group. Now with advancement in communication, the real time external quality control

program is also working for analysis of results.

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SKILLS TO BE LEARNT DURING MEDICAL LABORATORY

TECHNOLOGY COURSE

Demonstrate complete knowledge of all simple and advanced laboratory procedures

Ability to preserve, process and manipulate all types of biological samples in the laboratory

Using techniques appropriate for the tissue, produce sections (slides) for microscopic

examination. This includes fixation, trimming tissues, dehydration, microtomy, and staining.

Perform special stains on tissues and other biological samples when requested by the

Pathologist.

Using a cryostat, produce slides from frozen tissues for examination by fluorescent antibody

and other techniques.

Label slides with appropriate case number or other identification and match slides with lab

unit paperwork for delivery to pathologists.

Maintenance and use of microscopes

Demonstrate knowledge of medical terminology with special emphasis on writing and

understanding the laboratory reports.

Maintain necropsy room supplies.

Clean and disinfect necropsy room and equipment.

Maintenance of work records, tissue storage, up-to-date methodology and miscellaneous

duties.

Attend continuing education programs as funding and work load permits.

Assist in the preparation of cost accounting for histotechnology procedures.

Complete work in compliance with the quality assurance policies and procedures.

Maintenance of stock solutions, controls and equipment.

Ability to learn and master the operation of automated laboratory equipment.

The ability to trouble-shoot problems related to laboratory equipment.

Ability to communicate effectively both verbally and in writing.

Basic computer skills and knowledge of the Microsoft Office Suite.

Knowledge of record keeping.

The ability to analyze and modify the laboratory techniques according to the circumstances

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EQUIPMENTS AND GADGETS

Well Equipped Laboratory With Separate Sections For Microbiology, Histopathology,

Hematology And Chemical Pathology

Proper Ventilation, Lighting and Air conditioning System In The Laboratory

Pathologists Rooms

Broad Report Delivery and Collection Counter

Patient Waiting Room And Patient Sampling Room

Sample Labeling And Storage Room

Laboratory Furniture and Biosafety Cabinets

Water Filtration and Air Purification Systems

Lab Supplies, Such As Lab Disposables, Laboratory Instruments, Glassware

Simple And Advanced Laboratory Machinery, Controls, Assays, Standards, Periodicals,

Reference Materials, Computer Software, Scientific Databases, Document Management

Systems etc.

Autoclaves & Ovens

Tissue Processors

Centrifuges

Tissue Adhesives, Embedding & Mounting Mediums

Autopsy Set, Microtome & Surgical Blades, Knives.

Chemicals, Powdered Stains, Reagents And Diagnostic Test Kits

Immunoassay and Immunohistological Procedures Supplies

Alcohol, Buffers, Clearing Agents, Decalcifiers, Fixatives

Tissue Cassettes, Coverslips And Microscope Slides/Dishes

Special Stains & Immuno Controls

Filing, Labels And Transport Media, Pre-Filled Specimen Containers Etc.

Bacterial Cultures: Culture Media And Plates

Incubators For Microbiological Specimens

Stains: Histology, Cytology, Hematology, Misc.

Microscopes: Simple, Darkfield, Fluorescent, Inverted, Phase Contrast etc.

Elemental Analyzers :Chemistry and Electrolyte Analyzers

Mass Spectrometer and Spectrophotometer

Chromatography – Gas, HPLC, Co-Oximetry: Supplies and Titrators

http://websites.labx.com/rankin/listings.cfm?show=1&catid=1293&p=1





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ELISA Supplies

Electron Microscope / PCR / Immunoblotting/ Flow Cytometry Supplies In More

Advanced Labs

Other Equipments As Required

19

EXAMINATIONS

ASSESSMENT:

It will consist of action and the professional growth oriented student-centered integrated

assessment, with additional components of the internal assessment formative assessment

measurement based summative assessment.

STUDENT-CENTERED INTEGRATED ASSESSMENT.

It views students as decision makers in need of information about their own performance.

Integrated assessment is meant to student’s responsibility to decide what to evaluate as well as

how to evaluate, it encourages students to ―own‖ the evaluation and to use it as a basis for self

improvement. Therefore it tends to be growth oriented, student controlled, collaborative,

dynamic, contextualized, flexible and action oriented.

It will be based on,

Self assessment by the students

Peer assessment

Internal assessment by the faculty

SELF ASSESSMENT BY THE STUDENTS:

Each student will be provided with a predesigned self assessment form to evaluate his/her

level of comfort and competency in dealing with different types of education related

situations. It will be the responsibility of the student to correctly identify his/her areas of

weakness and to take appropriate measures to address to these weaknesses.

PEER ASSESSMENT:

The students will be expected to evaluate their peers after the monthly small group

meetings. These should be followed by a constructive feedback according to the prescribed

guidelines and should be non-judgmental in nature. This will enable students to become

good mentors in the future.

20

INTERNAL ASSESSMENT BY THE FACULTY:

The students are encouraged to confront their weaknesses and to remove them rather to

hide them from their teachers. It will be based on:

Punctuality

Practical work

Participation in interactive sessions

Regularly conducted class tests

FORMATIVE ASSESSMENT:

This will be helpful to improve the existing instructional methods and course contents in

use. This will be carried out through a pre designed form filled in by the students.

SUMMATIVE ASSESSMENT:

It will be carried out at the end of the programme to empirically evaluate the cognitive,

psychomotor and the affective domains in order to award the degree after successful

completion of the course.

21

ELIGIBILITY TO APPEAR IN THE FINAL EXAMINATIONS

Only those students who have completed four years of structured and supervised

training and have passed Ist, 2nd

and 3rd

professional examinations will be eligible to

take the final exams.

75% of the attendance with the certificate of the recommendation from the supervisor/

Head of the department/ Institution.

Application for admission to the university exam duly recommended by the Head of

the Institution.

A panel of two examiners, one internal and one external, will be appointed by UHS to conduct

the practical and the viva voce exam. Each component of the practical exam will be assessed by

both the examiners awarding marks independently. The final score will be an average of both

scores.

PASS PERCENTAGE AND OTHER REGULATIONS REGARDING EXAMS.

50% will be a passing score in each component.

The candidates have to pass in each component separately.

The candidate failing in one component will reappear in the same component

again.

The candidate has to pass the 1st , 2

nd and 3

rd professional examinations each, in

a maximum of 3 attempts.

The final examination will be cleared in a maximum of 4 attempts.

Only those candidates will be allowed to take the practical and the oral exam,

who pass in the theory exam.

The results will be announced as per UHS rules and regulations

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Annex : A

First Professional B.Sc Medical Laboratory

Technology Examination

Total Marks = 400 Pass Marks= 50%

Paper Subjects

Paper-I Basic Anatomy & Physiology

Theory 90 Marks

Internal Assessment 10 Marks

(05 + 05 in each Subject)

__________________

Total Marks=100

Paper-II Basic Biochemistry & General Pathology

Theory 90 Marks



__________________

Total Marks=100

Paper-III Islamic Studies / Ethics & Pakistan Studies

Theory 90 Marks


(06 marks in Islamic Studies/Ethics

04 marks in Pakistan Studies)

__________________

Total Marks=100

Paper-IV Behavioural Sciences & Computer Education

Theory 90 Marks



__________________

Total Marks=100

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FIRST PROFESSIONAL EXAMINATION OUTLINE OF TESTS

The First Professional examination shall be held at the end of first academic year (nine months of

teaching) and every candidate shall be required to take examination in the following subjects.

A candidate to pass in a subject shall have to obtain a minimum of 50% of total marks of each

part of the subject separately. The minimum number of marks required to pass the examination

for Islamic Studies/Ethics & Pakistan Studies shall be thirty three percent (33%) in each paper

separately and thirty three percent (33%) in aggregate.

Paper-I Basic Anatomy & Physiology = 100 marks

The examination in the subject of Basic Anatomy & Physiology shall consist of one

Theory Paper of three hours duration and of maximum 90 marks. Internal

Assessment will be of 10 marks.The syllabus to be covered is mentioned in

Appendix ―B‖.

Section I : Basic Anatomy = 50 Marks

There will be 45 MCQs and each question will carry 01 mark.

Internal Assessment will be of 05 marks.

Section – II: Basic Physiology = 50 marks

There will be 45 MCQs and each question will carry 01 mark

Internal Assessment will be of 05 marks

Paper-II Basic Biochemistry & General Pathology = 100marks

The examination in the subject of Basic Biochemistry & General Pathology shall

consist of one Theory Paper of three hours duration and of maximum 90 marks.

Internal Assessment will be of 10 marks. There will be two sections in this paper.

Section – I: Basic Biochemistry = 50marks



Section – II: General Pathology = 50marks



Paper-III Islamic Studies / Ethics & Pakistan Studies =100 marks

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The examination shall consist of one Theory Paper of 60+40=100 marks and 3

hours duration. The syllabus to be covered is mentioned in Appendix ―B‖.

Section-I : Islamic Studies/Ethics =60 marks.

This section shall have question on Islamic Studies in case Muslim candidates and on

Ethics in case of non-Muslim. There shall be 3 questions in this section of Theory and

there will be no choice.

Each question shall carry 18 marks.


Section-II : Pakistan Studies = 40 marks

This section shall have 3 questions on Pakistan Studies and there will be no choice. Each

question shall carry 12 marks.


Paper-IV Behavioural Sciences & Computer Education= 100 marks

The examination in the paper of Behavioural Sciences& Computer Education shall consist

of one Theory Paper of 90 marks and three hours duration. Internal Assessment will be of

10 marks. The syllabus to be covered is mentioned in Appendix ―B‖.

Section I : Behavioural Sciences = 50 marks



Section – II: Computer Education = 50 marks

There will be 45 MCQs and each question will carry 01 mark


.

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Second Professional B.Sc Medical Laboratory



Paper I Haematology and Blood Banking

Theory 90 Marks


Practical & Oral 90 Marks


__________________

Total Marks=200

Paper II Histopathology and Cytopathology

Theory 90 Marks




__________________

Total Marks=200

26

SECOND PROFESSIONAL EXAMINATION

OUTLINE OF TESTS

Total marks : 400 Pass marks : 50 %

The Second Professional Examination shall be held at the end of second year and shall

consist of the following subjects: The details of the syllabus is outlined in the Appendix B.

Paper-I:

Haematology and Blood Banking Total Marks : 200

Written paper:

The examination in the subject of Haematology and Blood Banking shall consist of one

written paper of three hours duration and of maximum 90 marks. Internal Assessment

shall be of 10 Marks

The written paper will consist of two sections as detailed below.

Section I : Haematology = 80 marks

There will be 8 short essay questions from the subject of Haematology and there

will be no choice. Each short essay question will carry 5 marks.


Section – II: Blood Banking = 20 marks

There will be 2 short essay questions from the subject of Blood Banking and there



Oral/ Practical Examination in the subject of Haematology and Blood Banking will consist

of maximum 90 marks. Internal Assessment shall be of 10 Marks.

27

Paper-II:

Histopathology and Cytopathology Total Marks : 200

Written paper:

The examination in the subject of Histopathology and Cytopathology shall consist of one

written paper of three hours duration and of maximum 90 marks. Internal Assessment

shall be of 10 Marks

The written paper will consist of two sections as detailed below.

Section I : Histopathology = 80 marks

There will be 8 short essay questions from the subject of Histopathology and there



Section – II: Cytopathology = 20 marks

There will be 2 short essay questions from the subject of Cytopathology and there



Oral/ Practical Examination in the subject of Histopathology and Cytopathology will

consist of maximum 90 marks. Internal Assessment shall be of 10 Marks.

28

Third Professional B.Sc Medical Laboratory



Paper I Chemical Pathology

Theory 90 Marks




__________________

Total Marks=200

Paper II Microbiology Including Parasitology

Theory 90 Marks




__________________

Total Marks=200

Paper III Immunology And Serology

Theory 90 Marks




__________________

Total Marks=200

THIRD PROFESSIONAL EXAMINATION

29

OUTLINE OF TESTS


The Second Professional Examination shall be held at the end of third year and shall

consist of the following subjects: The details of the syllabus is outlined in the Appendix B.

Paper-I:

Chemical Pathology Total Marks : 200

Written paper:

The examination in the subject of Chemical Pathology shall consist of one theory paper of

three hours duration and of maximum 90 marks. Internal Assessment shall be of 10 Marks.

The syllabus to be covered is mentioned in Appendix "B".

There will be 09 short essay questions from the subject of Chemical Pathology and there



Practical & Oral Examination in the subject of Chemical Pathology will consist of

maximum 90 marks. Internal Assessment shall be of 10 Marks

Paper-II:

Microbiology Including Parasitology Total Marks : 200

Written paper:

The examination in the subject of Microbiology Including Parasitology shall consist of one

theory paper of three hours duration and of maximum 90 marks. Internal Assessment shall

be of 10 Marks.


There will be 09 short essay questions from the subject of Microbiology Including

Parasitology and there will be no choice. Each short essay question will carry 05 marks.


Practical & Oral Examination in the subject of Microbiology Including Parasitology will

consist of maximum 90 marks. Internal Assessment shall be of 10 Marks

30

Paper III

Immunology and Serology Total Marks : 200

The examination in the subject of Immunology and Serology shall consist of one Theory

paper of three hours duration and of maximum 90 marks. Internal Assessment shall be of

10 Marks. There will be two sections of the paper.


Section I : Immunology = 50 marks

There will be 05 short essay questions from the subject of Immunology and there




Section – II: Serology = 50 marks

There will be 05 short essay questions from the subject of Serology and there will

be no choice. Each short essay question will carry 05 marks.



Practical & Oral Examination in the subject of Immunology and Serology

will consist of maximum 90 marks. Internal Assessment shall be of 10 Marks

31

Final Professional B.Sc Medical Laboratory



Paper I Medical Statistics and Research Methods

Theory 90 Marks


Research Paper 90 Marks


__________________

Total Marks= 200

Paper II Elective Subject

Theory 90 Marks




__________________

Total Marks=300

32

FINAL PROFESSIONAL EXAMINATION

OUTLINE OF TESTS


The Final Professional Examination shall be held at the end of fourth year and shall consist

of the following subjects: The details of the syllabus is outlined in the Appendix B.

Paper I

Medical Statistics and Research Methods Total Marks= 200

The examination in the subject of Medical Statistics and Research Methods shall consist of

one Theory Paper of three hours duration and of maximum 90 marks. Internal Assessment

shall be of 10 Marks.


There will be 09 short essay questions from the subject of Medical Statistics and Research

Methods and there will be no choice. Each short essay question will carry 05 marks.


Oral Examination in the research report will be of maximum 90 marks.

Internal Assessment shall be of 10 Marks

Paper II

Elective Subject Total Marks=300

The examination in the Elective Subject shall consist of one Theory Paper of three hours

duration and of maximum 90 marks. Internal Assessment shall be of 10 Marks.


There will be 09 short essay questions from the Elective Subject and there will be no

choice. Each short essay question will carry 05 marks.


Practical & Oral Examination in the Elective Subject will consist of maximum 180 marks.

Internal Assessment shall be of 20 Marks

33

APPENDIX – B

First Professional B.Sc Medical Laboratory


Paper-I BASIC ANATOMY & PHYSIOLOGY

Theory Marks: 90

Internal Assessment 05 Marks in each subject

Total Marks: 100

Pass Marks: 50%

Total study hours: 200

Syllabi and Course of Reading

Note: Syllabi and course of reading is divided into two parts. 100 hours will be allocated

for Sec I and 100 hours will be allocated for the Sec II. Question paper will carry 45 theory

marks for each part.

Section- I BASIC ANATOMY

(1) Introduction regarding Anatomical Nomenclature

Life span of a human being

Structural and functional organization

Terminology and body plan

Systematic Anatomy

Basic organization of the body

(2) Skin The structure of the hypodermis, dermis. and epidermis.

Superficial fascia and deep fascia

(3) The Musculoskeletal System:

Muscles, Bones and Joints Components of the Skeletal System

Description of Axial & Appendicular Skeleton

The process of bone ossification. Growth, Remodeling,and repair

Main features of the skull including all views

Shape and regions of vertebral column

Important features of the regional vertebrae

34

Bones of the thoracic cage, including the types of ribs.

The bones of the pectoral girdle and upper limb

The bones of the pelvic girdle and lower limb

Various types of joints and types of joint movement

connective tissue, components of the connective tissue matrix

Description of skeletal muscle, smooth muscle and cardiac muscle

Origin, insertion. synergist, antagonist and prime mover.

The movements of the arm, forearm and hand and the involved muscle

groups

Muscles of the trunk and the actions they accomplish.

Movements of the thigh, leg and foot with involved muscle groups

(4) The Nervous System Division of the Nervous System and the characteristics of each.

Central Nervous System

Peripheral Nervous System

Autonomic Nervous System

Special Senses

Anatomical pathways and decription of:

Olfactory system---- olfactory neurons

Hearing and Balance , structure of the outer middle and

inner ear

Taste ---- taste bud.

Visual --- chambers of the eye and structure of the rods and cones

The structure of a neuron, nerve, nerve tract, nucleus, and ganglion.

The components of a reflex arc and synapse

The three meningeal layers surrounding the central nervous system,

Cerebrospinal fluid and its circulation.

List the various cranial nerves

Various lobes of the brain and the cerebellum

(5) The Cardiovascular System

Anatomy of the Heart---- the size, shape and location of the heart and

Chambers, valves and their locations

The location of the coronary arteries

The structure of the conduction system of the heart.

Pulmonary and systemic circulation

The structure of arteries, capillaries and veins.

Major arteries and veins and the body areas, they supply

Lymphatic system tonsils, lymph nodes, the spleen and the thymus.

(6) Respiratory System

The anatomy of the respiratory passages, beginning at the nose and ending with the

alveoli.

The lobes of the lungs and the membranes that cover the lungs

Pleural cavity

The muscles of contraction of respiration

35

(7) The Digestive System

The structure of the organs that make up the digestive tract and their relations to

other organs in thoracic and abdominal cavity

Blood supply of the organs of the GI tract

Important secretory glands, the liver and pancreas (both exocrine and endocrine

components).

(8) Genito-Urinary System

The structures and organs of the urinary system and its relations with other organs

The structure of the nephron

Formation of Sex Cells

Organs of the Male Reproductive System

Organs of the Female Reproductive System

Recommended Books:

Essentials of anatomy and physiology by Seely, Stephens, and Tate

(4th

ed)

Anatomy & Physiology by Ross & Wilson

General Anatomy by Laeeq Hussain

General Anatomy by Dr Ghulam Ahmad

Anatomy by D. R. Johnson & K. L. Moore

Color Atlas of anatomy by Mc Minn

Lasts Anatomy by R.M.H Mcminn

Section- II BASIC PHYSIOLOGY

(1) Introduction To The Human Physiology

Functional organization---relationship between structure and function

of the human body

Homeostasis – its importance-- negative and positive feedback

mechanism

(2) Integumentary System

Functions of the skin, hair, glands and nails

Body temperature and its regulation

(3) The Musculoskeletal System:

36

Functions of the bones and muscles

Functional characteristics of Skeletal Muscle, Smooth Muscle and Cardiac Muscle

The events of muscle contraction and relaxation in response to an action potential

in a motor neuron.

Distinguish between aerobic and anaerobic muscle contraction.

Muscle hypertrophy and atrophy

(4) The Nervous System

Functions of the central nervous system,

The functional areas of the cerebral cortex and their interactions.

functions of the parts of the brainstem diencephalons, basal nuclei. Limbic system.

And cerebellum.

functions of various cranial nerves.

Functions of the somatic motor nervous system

Functions of the autonomic nervous system

The function of neurons, neuroglial cells.and their components.

Resting membrane potential and an action potential.

The function of a synapse and reflex arc

(5) The functions of the specialized sense organs

Eye---- physiology of site, accommodation, optic nerve and optic chiasma

Ear---- functions of the internal, middle and external ear

Physiology of the hearing and balance

Smell----------- physiology of olfactory nerve

Taste ---------physiology of taste

Location of the taste buds

physiology of speech

(6) The Endocrine System

Functions of the Endocrine System

Chemical Signals, receptors and hormones

The Endocrine Glands and their Hormones

Other Hormones

(7) Blood

Composition of Blood and Plasma

Functions of Blood

Formed Elements

Stages of cell development

Blood grouping

Coagulation mechanism

(8) The Cardiovascular system

37

Functions of the Heart

Electrical Activity of the Heart origin and propagation of cardiac impulse

Phases of the Cardiac Cycle

Heart Sounds

Regulation of Heart Functions--- intrinsic and extrinsic

Functions of the Peripheral Circulation

The Physiology of Circulation

o Pulmonary Circulation

o Systemic Circulation: Arteries

Veins

Local Control of Blood Vessels

Nervous Control of Blood Vessels

Regulation of Arterial Pressure

The function of Lymphatic System, tonsils, lymph nodes, the spleen and the

thymus.

(9) Respiratory System

Functions of the Respiratory System beginning at the nose and ending with the

alveoli.

Ventilation and Lung Volumes

Gas Exchange and gas transport in the blood

Rhythmic Ventilation

(10) The Digestive System

Functions of each organ of the Digestive System including major salivary glands

Movements and Secretions in each organ of the Digestive System and their

regulation

Physiology of Digestion, Absorption, and Transport

(11) Genito-Urinary System

Urine Production, Urine Movement

Regulation of Urine Concentration and Volume

Body Fluid Compartments

Regulation of Extracellular Fluid Composition

Regulation of Acid-Base Balance

Physiology of Male Reproductive system—spermatogenesis and reproductive

glands, hormones and their regulations

Physiology of Female Reproductive system--- ovulation, hormones and their

regulations

(12) Immunity

38

Define immunity, Innate Immunity, Adaptive Immunity

Antigens and Antibodies

Primary and secondary responses to an antigen

Antibody-mediated immunity and cell-mediated immunity

Role of lymphocyte in immunity regulation

Recommended Books

Essentials of Anatomy and Physiology by Seelay, Stephens and Tate. 4th

edition

Ross & Wilson Anatomy and Physiology.

Human Physiology. Stuart Ira Fox. 7th

edition

Text Book of Medical Physiology Guyton

Essential of Medical Physiology Vol.I & II by Mushtaq Ahmad.

Lecture notes on human physiology by Bray JJ, Cragg, PA MacKnight

39

PAPER II: BASIC BIOCHEMISTRY AND GENERAL

PATHOLOGY

Theory Marks: 90


Total Marks: 100

Pass Marks: 50%





marks for Basic biochemistry and 45 theory marks for General Pathology.

Section -I: BASIC BIOCHEMISTRY

Physiochemical Principles

Hydrogen ion conc. and pH notation

Acidity & Alkalinity

Indicators & Buffer solutions

PH and its determination

The colloidal state

Absorption

Structure and function of cell membrane and movement of materials across cell

membrane

Osmosis & Osmotic pressure

Surface tension

Viscosity

Carbohydrates

Introduction and classification of carbohydrates

Some important monosaccharides, disaccharides and polysaccharides

Regulation of blood glucose level

Definition and end products of

Glycolysis

Citric acid cycle

Glycogenolysis

Glycogenoses

Gluconeogenesis

Proteins and Amino Acids

Introduction, importance, classification and properties of proteins

Entry of amino acids into cells and peptide linkage

40

Special sources of proteins

Lipids

Introduction, Classification and Function of lipids

Biosynthesis of fatty acids, natural fats or triglycerides

Fatty acid oxidation

Vitamins and Minerals

Classification of vitamins

Fat soluble vitamins and Water soluble vitamins

Deficiency effects

Enzymes

Introduction, Classification Chemical nature and properties of enzymes

The mechanism of enzyme reactions

Factors affecting the enzyme activity

Important coenzymes and their actions

Regulatory enzymes

Nutrition and Dietetics

Balanced diet

Role of carbohydrates, fats and proteins, their dietary sources and uses in the

body

Quantitative and qualitative daily requirements of carbohydrates, fats, proteins,

vitamins and minerals

Recommended Books

Review of Biochemistry by Lippincott

Essential of Medical Biochemistry Vol.I & II by Mushtaq Ahmad.

Fundamentals of Biochemistry by D. Voet, J.G.Voet (1999)

Text Book of Biochemistry with Clinical Correlations by T.M.Devlin.

Modern Experimental Biochemistry by R.F.Boyer.

Section -II: GENERAL PATHOLOGY

Cell Injury and adaptation

Cell Injury

Reversible and Irreversible Injury

Fatty change, Pigmentation, Pathologic calcification

Necrosis and Gangrene

Cellular adaptation

Atrophy, Hypertrophy,

Hyperplasia, Metaplasia, Aplasia

41

Inflammation

Acute inflammation --- vascular changes, Chemotaxis, Opsonization and

Phagocytosis

Enlist the cellular components and chemical mediators of acute

inflammation

Differentiate between exudates and transudate

Chronic inflammation

Etiological factors, Granuloma

Cell repair and wound healing

Regeneration and Repair

Healing--- steps of wound healing by first and second intention

Factors affecting healing

Enlist the complications of wound healing

Haemodynamic disorders

Define and classify the terms Edema, Haemorrhage, Thrombosis,

Embolism, Infarction & Hyperaemia with at least two examples of each.

Define and classify Shock with causes of each.

Describe the compensatory mechanisms involved in shock

Describe the possible consequences of thrombosis

Describe the difference between arterial and venous emboli

Neoplasia

o Define the terms Dysplasia and Neoplasia with examples of each

o Enlist the differences between benign and malignant neoplasms

o Enlist the common etiological factors for neoplasia

o Define and discuss the different modes of metastasis

Recommended Books

Pocket companion to Robbins. Pathologic basis of disease Cotran, Kumar,

Collins

42

PAPER III ISLAMIC STUDIES/ETHICS &

PAKISTAN STUDIES

Total Theory Marks:100


Note: Syllabi and course of reading is divided into two parts50 hours will be allocated for Sec I

and 50 hours will be allocated for the Sec II. Question paper will carry 54 theory marks for

Islamiyat and 36 theory marks for Pakistan studies. Non muslims can appear in the subject of

Ethics instead of Islamiyat. Candidates can attempt paper in Urdu or English.

Internal Assessment will be of 10 Marks in total.

ETHICS (FOR NON MUSLIMS)

Theory Marks: 54

Internal Assessment :06 Marks

Total Marks : 60

Pass Marks: 33%

1. Ethical Teachings of world religious with special reference to Budhish, Judaism

Christianity and Islam.

2. 100 ethical precepts from Quran and Sayings of the Prophet.

The Arabic text of Holy Quran and Ahadith would not be advisable for inclusion in

the syllabus for the Non-Muslims. Instead the teachings of Holy Quran and sunnah

relating to the following topic should be explained in English or Urdu, hence,

questions about this portion of the syllabus should be based on the subject-matter,

and not on the texts.

Virtues

Duty towards parents: respect for human life, unity of mankind, peace, justice,

tolerance, beneficence, pity, contentment, chastity, meekness, repentance, social

solidarity, individual accountability, moral excellence, patience and perseverance,

forgiveness,

Vices

Arrogance, ostentation, extravagance, misery, greed, jealousy, suspicion,

backbiting, coercion, hypocrisy, bribery, obscenity and immodesty.

1. Promotion of moral values in society.

2. Attitude of Islam Towards Minorities

43

ISLAMIYAT

Theory Marks: 54


Total Marks : 60

Pass Marks: 33%

.

46

Section II

PAKISTAN STUDIES Theory Marks : 36


Total Marks: 40

Study Hours: 50

Pass Marks: 33%

48

Paper-IV

BEHAVIOURAL SCIENCES & COMPUTER EDUCATION

Theory Marks: 90


Total Marks: 100

Pass Marks: 50%


Syllabi and course of reading



marks for Behavioural Sciences and 45 theory marks for Computer Education .

Section -I:

BEHAVIOURAL SCIENCES Total Marks: 50

Pass Marks: 50%

Study hours: 100 hrs

1. Introduction to Behavioural Sciences and its importance in health.

Bio-Psycho-Social Model of Health Care and the Systems Approach

Normality vs Abnormality

Importance of Behavioural sciences in health

Desirable Attitudes in Health Professionals

2. Understanding Behaviour

Sensation and sense organs

Describe sensation, sense organs/special organs

Perception

Define perception, what factors affecting perception

Attention and concentration Define attention and concentration. What factors affecting them

Memory

Define memory and describe its stages, types and methods to improving it

Thinking

Define thinking; describe its types and theories

What is cognition and levels of cognition?

Discuss problem solving and decision making strategies

Communication Define communication. What are types, modes and factors affecting it. Describe

ways to recognize non-verbal cues. Characteristics of a good communicator

49

3. Individual Differences

Personality

Define personality. What factors affect personality development? How personality can be

assessed? Influence of personality in determining reactions during health, disease,

hospitalization, stress

Intelligence.

Define intelligence and the various types of intelligence.

What factors affect it and how it can be assessed?

Emotions

Define emotions. What are the various types of emotions?

Emotional Quotient (EQ)- concept & utility

Motivation

Define motivation and what are the types of motivation?

4.Learning Define learning, Principles of learning, modern methods and styles of learning,

types of learners, Strategies to improve learning skills

5. Stress and Stressors

Define and classify stress and stressors

Relationship of stress and stressors with illness

6. Life Events Concept of life events and their relationship with stress and illness

7. Stress Management What is coping skills

What is conflict and frustration?

What is concept of adjustment and maladjustment?

8. Interviewing / Psychosocial History Taking Define, types of interview and listening

Skills of interviewing and listening

9. Allied Health Ethics-Hippocratic oath Do’s and Don’ts

What is the concept of Allied Health ethics?

10. Culture and Allied Health practice Concept of group, its dynamics

Attitude, value, belief, myths, social class, stigma, sick role and illness, health belief

models

11. Psychological reactions Grief and bereavement, Family and illness

Dealing with difficult patients

50

What are the psychosocial aspects of illness, hospitalization, rape, torture, terminal

illness, death and dying?

Psychosocial issues in Emergency Departments, Intensive Care and Coronary Care

Units, Operating Theatres, Cancer wards, Transplant Units, Anaesthesia

12. Breaking Bad News Introduction, Models, Methods, Death of the patient, abnormal baby, intractable

illness

13. Pain, Sleep, Consciousness Concept of pain.

Physiology of pain,

Altered states of consciousness.

14. Communication Skills Counseling,

Crisis Intervention

Conflict Resolution

Principles of effective communication, active listening, the art of questioning

The art of listening.

Good and bad listener.

Counseling: Scope, Indications and Contraindications,

Steps, Do’s and Don’ts, How to deal with real life crisis and conflict

situations in health settings

Section II:

COMPUTER EDUCATION Total Marks: 50

Pass Marks: 50%

Study hours: 100 hrs

Introduction To Computers

Definition

Usage and functionality of computers

Limitations of Computers

Classification of Computers

Basic Components of Computers

Hardware

Software

System Software

Application Software

Equipment’s/devices in Personal computer system

Input devices

Output devices

Storage devices

The processor

51

Microsoft Windows

Introduction to MS-Windows Arranging, Moving and Resizing Windows.

Identifying the components of desktop.

Moving, Changing and Closing Windows.

Crating, Opening and Deleting items and folders.

Working with My Computer

Deleting and Resume Print Jobs.

Using Control Panel

Working with Accessories.

Microsoft Office

Microsoft Win Word

Microsoft Excel

Microsoft Power Point

Database

Internet and Email

Introduction To Outlook Express

Using Internet Explorer

52

Second Professional B.Sc Medical Laboratory


Paper I

HAEMATOLOGY AND BLOOD BANKING

Theory Marks 90


Practical Marks 90


Total Marks 200

Pass Marks: 50%

Theory hours: 200

Practical Hours: 400


Sec- I

HAEMATOLOGY

Course Outlines:-

A. Orientation and introduction to study of Haematology.

B. Origin and Development of Blood Cells, Maturation of Erythrocytes and

erythrocyte count.

C. Haemoglobin.

D. Haematocrit, Erythrocyte sedimentation rate.

E. Maturation of Leukocytes, Leukocyte Count.

F. Maturation of Thromobocytes, Preparation of Blood Smears and Differential Blood

Count.

G. Total Eosinophil Count and Cerebrospinal fluid count.

H. Pathology of Erythrocytic series, Including abnormal Haemoglobin syndromes and

Indices.

I. Reticulocytes, Haemostasis and Blood Coagulation.

K. Laboratory Diagnosis of Coagulation defects.

L. Special Stains in Haematology.

M. L.E Phenomenon and Continuation of Bone Marrow study.

1. Introduction to Haematology.

a) Review of vascular system and Blood Constituents.

b) Methods for Securing Blood.

c) Method for securing Bone Marrow.

Origin and Development of Blood cells, Maturation of Erythrocytes and erythrocyte

count.

53

1. Blood formation:

a) Intrauterine.

b) Extrauterine.

2. Factors which govern Haematopoiesis.

3. Principles of Normal cell Maturation.

4. Erythrocytes:-

a) Definition.

b) Maturation.

c) Factors required for erythropoeisis.

5. Enumeration of Erythrocytes:

a) General principles of count.

b) The hemocytometer.

c) The red cell diluting pipette.

d) Diluting fluids.

e) Sources of error.

f) Normal Values.

Haemoglobin:

1. Definitions of terms.

2. Chemistry of Haemoglobin.

3. Metabolism.

4. Compounds of Haemoglobin.

5. Haemoglobinometery.

Haematocrit, Erythrocyte Sedimentation Rate:

1. Haematocrit.

a) Definition and principle of test procedures:

1. Micro 2. Macro.

b) Sources of Error.

c) Normal Values.

d) Significance of abnormal findings.

e) Correlation of Haemoglobin, Haematocrit, and Erythrocyte Count.

2. Erythrocyte Sedimentation Rate:

a) Principle of Test Procedures

b) Methods

c) Sources of Error.

d) Normal Values.

e) Significance of Abnormal Values.

Maturation of Leukocytes, Leukocyte Count.

1. Introduction:-

a) Definition.

b) Origion.

c) Functions and biological Properties.

2. Maturation of Granulocytic Series.

54

a) Criteria for identifying cells and description of cells.

3. Maturation of Lymphocytic Series.

4. Maturation of Monocytic Series.

5. Enumeration of Leukocytes:-

a) General Principle of haemocytometer method.

b) The Haemocytometer.

c) Diluting Fluids.

d) Sources of Error.

e) Normal Values.

Maturation of Thrombocytes, Preparation of Blood Smears and Differential Blood

Count.

1. Maturation of Thrombocytes:

a) Description of Cells.

2. Preparation of Blood Smears.

a) Preparation of various types of blood smears.

3. Fixation.

4. Staining.

a) Types of Stains.

b) Criteria for good smear stained by Wright’s method.

c) Sources of error.

5. Examination of stained smears:

a) Define differential Count.

b) Observation of Erythrocytes.

c) Number of Platelet estimated.

d) Tabulation of Leukocytes.

e) Classification of leukocytes and normal values:-

1. Arneth Count.

Total Eosionphil Count and Cerebrospinal fluid count:

1. Funtions of Eosinophils.

2. Significance of increased and decreased values.

3. Principle of the staining method for total eosinophil count.

4. Equipment used in eosinophil count.

a) Haemocytometer.

5. Normal Values for the method used.

6. Cerebrospinal fluid:

a) Definition.

b) Sources.

c) Functions.

d) Collection :- The Lumbar puncture.

e) Laboratory studies.

1. Necessity of maintaining sterlity.

2. Necessity of imidiate examination.

3. Gross Observation.

f) Cytologic studies.

1. Principle of the cell count.

55

2. Normal values and significance abnormal findings.

Pathology of Erythrocytic series, Including abnormal Haemoglobin syndromes and

Indices.

1. Brief review of maturation of erythrocytes and haemoglobin metabolism.

2. The Indices.

3. Abnormal Forms:

a) Abnormalities on shape

b) Abnormal inclusions.

c) Variation in haemoglobin content and staining properties.

d) Diagnosis and differential diagnosis of

Iron deficiency anaemia, megaloblastic anemia, hemolytic

anemias, sideroblastic anemia, thalassemias, sickle cell

anaemias, aplastic anaemias.

Polycythemias

Reticulocyte Count, Fragility of Erythrocytes, Sickle cell studies:

1. Reticulocyte Counts:

a) Method of smear making for reticulocyte count

b) Normal values for adults and infants.

c) Means of demonstrating reticulocytes, Principle of the staining

reaction:

d) Sources of error.

2. Fragility of Erythrocytes:

a) General Considerations

1. Response of the erythrocytes to the Hypertonic and

Hypotonic solutions.

b) Determination of fragility of erythrocytes:

Effect of small variations in shape and size of erythrocytes on

the fragility.

3. Sickle Cell studies:

a) Principle of tests for sickle cell studies

b) Laboratory Diagnosis.

1. Sealed whole blood method.

2. Sodium metabisulfite method

Thrombocytes, Homeostasis, and Blood Coagulation:

1. Thrombocytes:

a) Physical properties

b) Review of Maturation

c) Functions

d) Enumeration of Platelets

2. Haemostasis:

a) Haemorrhage

b) Haemostasis

Special stains in Haematology:

1. Peroxidase stain

2. Sudan Black B

3. Periodic Acid Schiff (PAS) Stain

4. Feulgan reaction

56

5. Histochemical techniques for alkaline phosphatase

6. Miscellaneous stains used in Haematology:

a) Giemsa.

b) Prussian blue reaction

L.E Phenomenon and continuation of Bone Marrow study.

1. L. E phenomenon and techniques of L.E cells preparation.

2. Bone Marrow Examination

a) Bone marrow aspiration and trephine biopsy.

b) Bone marrow differential count.

Sec- II

BLOOD BANKING

Course Outlines:

A. History of Blood Transfusion

B. Antigen – Antibody theory

C. Classification of antibodies

D. Blood Groups A.B.O systems

E. Rh – Hr typing, Agglutinogen-Agglutinin theory

F. Cross matching prcedure

G. Other Blood group system

H. Transfusion Reactions

I. Antiglobulin tests

J. Rh antibody tests

K. Erythroblastosis foetalis (Review of clinical and Laboratory findings)

L. Donors

Description of Outlines

History of Blood Transfusion

Antigen Antibody theory:

1. Antigen

2. Antibody

3. Immunization

Classification of Antibodies:

1. Preciptin

2. Lysin

3. Agglutinoid

4. Complete/Bivalent

5. Antiglobulin test

Blood groups ABO systems:

1. Agglutinogen – definition

2. Agglutinin – definition

3. Landsteiner’s Postulates

4. Sub groups of ―A‖ and ―B‖

5. Techniques for blood grouping-slide test

6. Techniques for reverse blood grouping-tube test.

7. Sources of error, controls in blood bank, general.

57

8. Low titered groups ―O‖ blood

Rh – Hr Typing: Agglutinogen Agglutinin theory:

1. Agglutinogen definition

2. Agglutinin definition

3. Cause of sensitization to the Rh – Hr factor

4. Techniques for Rh typing

5. Sources of error Controls

Cross Matching prcocedures:

1. Purpose of ccrossmatch

2. Methods of crossmatching

3. Crossmatching problems

a) Rouleaux

b) Cold Agglutinins

c) Hyperproteinemia and hyperglobulinemia

Other Blood Group Systems

Transfusion reactions

Antiglobulin tests:

1. Direct Coomb's

2. Indirect Coomb's

3. Sources of error, controls

Rh Antibody tests:

1. Screening tests using a cell panel:

a) Slide test

b) Tube test

2. Titrations

Erythroblastosis Foetalis:

1. Causes:

a) Due to Rh incompatibility

b) Methods of testing

c) Due to ABO incompatibility

d) Methods of testing

58

PAPER II

HISTOPATHOLOGY AND CYTOPATHOLOGY

Theory Marks 90


Practical Marks 90


Total Marks 200

Pass Marks: 50%

Theory hours: 200



Sec- I

HISTOPATHOLOGY

Course Outlines:

o Brief history of microscopy

o Parts of a microscope

o Types of microscope. Classification and their uses.

o Nature of light, Concepts of amplitude, Wavelength and Phase.

o Perception of color and brightness.

o Refraction, formation of images.

o Merits and Demerits of achromatic and apochromatic objectives.

o Immersion objectives.

o Specification of objective magnification, focal length, tube length,

resolution, numerical aperture etc. Calculation of the resolution and

magnification

o Care and Cleaning of the Microscope

o Introduction to common Histological Techniques:

o Examination of fresh material. Supravital staining. Examination of fixed

material.

o Fixation:

o The purpose of fixation, common fixative used for the histological

techniques.

o The Paraffin method of sectioning tissue:

59

o Advantages and disadvantages of the paraffin method. Dehydration of

tissues. Clearing of tissues Infiltration with paraffin. Paraffin block making.

Section cutting with a rotary microtome. Fixing paraffin section to slides.

o Microtome and Microtome Knives:

o Grinding and stooping of microtome knives.

o Cleaning and lubrication of the microtome.

o The Freezing Method of Sectioning:

o Advantages and disadvantages of freezing method.

o Common techniques of freezing tissues.

o Cutting sections with a freezing microtome.

o Stains:

o Object of staining.

o Classification of stains.

o Acids and basic dyes.

o Basophilic and acidophilic tissue components.

o Routine Haematoxyline-Eosin Staining Of Paraffin Sections

o The procedure of haematoxyline-eosin staining and mounting sections.

o The relation of various steps in this procedure.

o Special Staining Techniques

o GMS, Mucicarmine and Alcian Blue.

o Stains for Connective Tissue Elements

o Mallory’s connective tissue stain.

o Aldehyde fuchsin and Verhoff’s stain for elastic fibers

o Gordon + Sweet stain for reticular fibres

o Toluidine blue staining of mast cells

o Von- Geison, Masson’s Trichome

o Stains for Nervous Tissues:

o Nissel Stain. Stains for myelin.

o Histochemical demonstration of lipids:

o Choice of fixative.

o Choice of sectioning Technique.

o Sudan Black B Stain.

o Staining for frozen section.

o Histochemical demonstration of glycogen:

o Choice of fixative and sectioning

o Best’s Carmine staining for paraffin sections.

60

o Demonstartion of :

o Calcium, Iron, Melanin, Muscle Tissue PTAH, Amyloid Material, Mucinous

Material

o The PAS Technique:

o The Schiff reaction.

o Significance of the Schiff reaction.

o Procedure of the PAS staining.

Special Gross Anatomical Techniques

Preserving and mounting gross anatomical specimen:

Preservative fluids: Kaiserling Solution I & II.

Mounting specimens in fluid media.

Mountings specimens in plastics

Immunohistochemistry

Introduction and significance

Methods of Immunohistochemistry: Direct and Indirect

PAP / Avidin Biotin method

Steps involved in Immunohistochemistry (starting from dewaxing to the

final chromogen application)

Significance of interpretation of the results: scoring/ staining intensity

Antigen retrieval methods

Types of fixatives, buffering media, enzyme labels and chromogens used in

Immunohistochemistry.

List of commonly used tumor markers in different diseases and their clinical

utility.

Biopsy and types of biopsies

Merits and demerits of different types of biopsies

Fixation methods with salient gross and microscopic morphological changes in

common diseases of:

Gastrointestinal Tract

Genitourinary System (Male and Female)

Respiratory tract

Brain and spinal cord

Skin and subcutaneous tissues

Heart and blood vessels

Lymphatic system including tonsils, lymph nodes, the spleen and thymus

61

Sec -II

CYTOLOGY

o Cell and its structure, classification of cells and tissues.

o Basic principles of exfoliative cytology

o Exfoliation, sites from which exfoliated cells can be obtained and methods

for obtaining them.

o Pathologic processes affecting cell morphologies:

Inflammation, Repair and regeneration, benign and malignant tumors.

o Female genital tract.

Methods for obtaining smears and their fixation

Pap’s and Giemsa’s staining

Normal cells of female genital tract

Abnormal cells other than malignant cells

Diagnosis of carcinoma of male genital tract.

o Respiratory tract:

Method for obtaining smears and their fixation

o Cytologic techniques for

Urinary tract

G.I. tract

Circulating blood and aspirating smears.

o Immunocytochemistry

Introduction and significance

Methods of Immunocytochemistry: Direct and Indirect

PAP / Avidin Biotin method

Steps involved in Immunocytochemistry (starting from fixation to

the final chromogen application)

Antigen retrieval methods

Types of buffering media, enzyme labels and chromogens used in

Immunocytochemistry

62

Third Professional B.Sc Medical Laboratory


PAPER I

CHEMICAL PATHOLOGY

Theory Marks 90


Practical Marks 90


Total Marks 200

Pass Marks: 50%

Theory hours: 100



Course Outlines:

Chemical Changes in Gastrointestinal tract:

Digestion and digestive enzymes, their control and mechanism of

secretion, bile Absoprtion of water, Minerals, Fats, Carbohydrate and

proteins.

Urine:

Composition of glomerular filtrate and change which occure in the

tubules, normal urine, physical properties, and composition, pathological

constituents and their determination, kidney function tests.

Minerals:

Metabolism of Na, Ca, P, Fe, Cu, and Co, Electrolyte balance and

imbalance.

Water Balance:

Distribution of body fluids, were intake and output, dehydration and

edema.

Hormones:

Parathyroid effects on Ca and phosphorus metabolism, Thryoid

functions and chemistry, effects of hormones at metabolism.

Quantitative Analysis of Urine:

Amino acids, Bence-Jones proteins, Calcium, Coproporphyrins,

creatinine, glactose, phosphates, nitrogen, PH, specific gravity, Ca, p,

Oxalates and urates. Determination of

Urea (qualitative and Quantitative) pathological Constituents:

Sugar, Albumin, Ketone bodies, Blood, Bile pigments.

63

Renal function tests :-

1. Renal function tests used in clinical investigation.

a) Inulin and Creatinine clearance (GFR).

b) Para-aminohippurate clearance (renal plasma flow).

c) Maximum rate of tubular excretion of P-amino-hippurate (Tr-PAH)

(measures excretory function of renal tubules).

d) Maximum rate of reabsorption of glucose (Tmg) (measures reabsorption

function of renal tubules).

1. Classification of Renal Function Tests.

2. Classification of renal function tests used in clinical assessment of renal

function.

a) Blood urea nitrogen.

b) Serum Urea.

c) The phenolsulfonphthalein (PSP) test.

d) Serum creatinine

e) Creatinine Clearance

f) Serum Uric Acid

g) Electrolytes

Outline J :-

Bile Pigments and Urobillinogen in urine.

1. Two types of bile pigments and their characteristics.

a) constituents and derivation of bile which appear in the urine.

2. Derivation of Bile in urine.

a) Routine screen test for billirubin.

b) Methods:- 1-Foam test, 2-Harrison focuchet method, 3-

Harrison’s spot test, 4-Icto test (ames company), 5-Observant for

standing of element of urine sediment.

3. Determination of Urobilin in urine.

4. Determination of urobillinogen in the urine.

Semi quantitative methods of Watson or of Wallace and Diamond.

Outline K :-

Haemoglobin, Myoglobin and Haemosiderin in Urine :

1. Haematuria.

2. Detection of Haemoglobin in urine.

a) Spectroscopic examination.

b) Banzidins test.

c) Guaiac test.

d) Tablet test.

3. Myglobinuria.

a) differentiation or separation from Haemoglobin.

i. spectroscopic examination.

ii. spectrophotometery.

64

iii. Precipitation by 80% saturated ammonium sulfate.

4. Haemosiderin :

a) prussian blue reaction.

Outline L :-

Other chemical tests :

1. Indicanuria :

a) Obermayer’s test.

2. Porphyrinuria :

a) Identification of Perphobilinogen.

b) Identification of uroperphyrins.

c) Identification of coproperphyrins:

Method of Schwitz, Zeir and Watson.

3 Melanin:

a) Ferric chloride test.

b) Bromine Water test.

c) Method of Blackberg and Wanger.

d) The Thormahlen test.

4. Chlorides:

a) Frantus test.

b) Method of Schales and Schales.

5. Phenylketonuria:

a) Ferric Chloride test.

b) Phenistix.

6. Metabolites of Salicylate and PAS:

a) Screening test for Salicylates and PAS.

i) Ferric Chloride ii) Phenistix.

Outline M:-

Urinary Calculi :

1. Observations to be made of gross appearance.

2. Reagents and Scheme for detection.

Outline N:-

Lipid Profile

Liver Function Tests

Cardiac Profile

Outline O:-

Hormones:

1. Parathyroid effects on Ca and Phosphorus

2. Thyroid Functions and Chemistry

3. Insulin effects on Glucose metabolism, secretion, structure.

Hyperglycemia, Diabetes Mellitus. Antagonistic hormones

65

4. Male and Female sex hormones. (Testicular/ Ovarian/ Placental

Hormones). Synthesis, secretion, actions, metabolism. A little

information about Amenorrhea, Oligomenorrheoa, Hirsutism, Virilism,

Spermatogenesis, Ovulation, Climacteric.

5. Anterior and Posterior pituitary hormones, hypothalamic hormones.

Positive and negative feedback.

6. Adrenal Glands. Hormones of the adrenal medulla and cortex. Hypo and

Hyper secretion. Cushing’s syndrome. Addison’s disease.

Pheochromocytoma.

Practical:

Quantitative Analysis of Blood:

Use of the visual colorimeter and photoelectric colorimeters, instruction on calculation of

the concentration of the substances determined. The following blood analytical procedures

will be taught.

Creatinine, Fatty acids, Phosphates, iron, plasma protein, calcium Cholestrol, Glucose,

Urea.

PAPER II

MICROBIOLOGY INCLUDING PARASITOLOGY

Theory Marks 90


Practical Marks 90


Total Marks 200

Pass Marks: 50%

Theory hours: 100



Course Outlines:

Introduction to microbiology.

Interaction between host and infectious agent

Purpose of infectious agent in nature

What is the virulence role of environment in infection?

o Gram positive cocci. Taxonomy. General characteristics.

o Clinical significance of bacteria.

o Isolation and identification of bacteria.

o Staphlococci and related gram positive cocci.

o Strepto cocci. Entero cocci. Enterobacteriaceae: Escherichieae.

Edwardsielleae.Salmonelleae.Citrobacteriaceae. Klebsielleae. Proteacae.

Yersinieae, Erwinieae.

o The Non-fermentative gram negative bacilli.

o Curved gram-negative bacilli.

o Vibrionaceae and campylo- bactericaeae.

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o Fastidious gram negative bacilli

o Hemophilus. sp. Actino bacillus sp. Pasteurella.

o Dysgonomonas species. Streptobacillus Moniliformis brucella species.

Bartonella. sp. Bordetella sp.Legionella. Neisseria species and moraxella

catarrhalis. Aeobic and gram positive bacilli. Listeria monocytogenes.

Erysipelothrix.

o Corynebacterium. sp. Gardnerella vaginalis. Lactobacillus sp.

o Aerobic actinomycetes. Nocardioform group the maduromycetes.

o Streptomycetes.

o Thermophilic actinomycetes.

The Anaerobic bacteria, Anaerobic. non. Spore forming gram-

positive bacilli.

Acinomycetes.sp, Lactobacillius.

Clostridum species.

Mycoplasmas and Ureaplasmas.

Mycobacteria.

Spirochetal infections: Treponema. Borrelia,Leptospira,Spirillum

minus(rat bite fever)

o Mycology: o Clinical categorization of fungal infections. Laboratory approach to

diagnosis of fungal infection. Identification of dermatophytes.

o Laboratory identification of yeast.

o Parasitology:

o Clinical manifestations of parasitic disease. Life cycle. Prevention.

o Collection. Transport of specimen and its identification in laboratory.

o Intestinal (1 )Protozoa. Amoeba. Flagellates. Ciliates.(2) Nematodes.

(3)Cestodes (4) Trematodes(5)Blood and tissues parasites.

o Virology: o Introduction. Clinical manifestation of viral infection.

o Diagnosis of viral infection.

o Infections with Chlamydia species.

o Infections with Rickettsia. Coxiella. Ehrlichia and anaplasma.

o Sterilization

Laboratory Exercises:

a) Each lecture will be followed by two hour practical class where the student will

apply their theoretical knowledge in the understanding of related microbiological

investigations, which have been proved useful for the diagnosis of human diseases.

b) During the other laboratory sessions, the students will be engaged in the preparation

of media, the sterilization of glass ware, Antigens, Antibodies, Vaccines –

haemolysin, permanent slides, laboratory reagents and also to assisting postgraduate

students in the isolation of micro – organisms from clinical materials.

PAPER III

67

IMMUNOLOGY AND SEROLOGY

Theory Marks 90


Practical Marks 90


Total Marks 200

Pass Marks: 50%

Theory hours: 100



Sec- I

IMMUNOLOGY

General Immunology

o Innate and adaptive immunity

o Antigen and antigenecity

o Antibodies and immunoglobulins

o Cell mediated and humoral immunity

o Immune system

o Hypersensitivity

o Autoimmunity

o Immune mediated diseases

o Immunization

o Immune deficiency

Immunological Techniques

General considerations

Agglutination reactions

Precipitation reactions

Immunodiffusion techniques:

Double diffusion

Single radial immunodiffusion .

Immunoelectrophoresis

Complement Fixation test

Immunoflourescence

o . Direct

o . Indirect

Enzyme Immunoassay (EIA)

Immunochemical Techniques:

Quantitation of IgG, IgA, IgM, IgD, and IgE in serum and other body

fluids

Immunoelectrophoretic analysis of serum immunoglobulin abnormalities

Detection/quantitation of Bence-Jones protein in the urine

Cryoglobulin determination and analysis

Tests for circulating immune complexes by immunochemical methods

Immunochemical and electrophoretic analysis of CSF

Measurement of overall complement function

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Total haemolytic and alternative pathway titrations of complement

components (especially C3, C4, Factor Band C1 esterase inhibitor)

Electrophoretic examination for altered complement components Other serum protein determinations including acute phase proteins (CRP

etc.), Carcinoembryonic antigen, a-fetoprotein and protein clearance

ratios

Pregnancy tests on urine.

Immunohistological Tests:

Detection of antigens, antibodies, Immunoglobulins and complement

components deposited in pathological lesions, particularly in the kidney

and skin;

Characterization of plasma cells and lymphocyte types in relevant tissue

biopsies, using immunofluorescent and enzyme-labelled techniques.

Sec -II

Serology

o Introduction of immune system

o Antigens, definition types examples

o Anti-bodies definition, types functions, structures

o Antigen anti-body reactions

Serological Techniques

Tests for circulating antibodies to autoantigens in tissue sections by indirect

immunofluorescence and enzyme-labelled techniques

Tests for antibodies to other autoantigens by agglutination, precipitation,

complement fixation and radioimmunoassay

Tests for antibodies to non-microbial environment and fool allergens

Tests for antibodies to selected microbiological antigens

Tissue Typing For HLA-Antigens: Serological And DNA Based

o Principles of QUALITY CONTROL and the use of reference preparations

as laboratory standards

o

1. Antigen-Antibody reaction techniques

2. ELISA Technique

3. Widal test:

4. Typhidot test

5. Brucella Agglutination test.

6. Antist reptolysin O titre.

7. Bacterial Haemagglutination test.

8. Paul Bunnel reaction.

9. RA Factor Test

10. CRP test.

11. RPR test

12. VDRL test.

13. Complement fixation test

14. PCR

69

Final Professional B.Sc Medical Laboratory


Paper I

MEDICAL STATISTICS AND RESEARCH METHODS

Theory Marks 90


Practical Marks 90


Total Marks 200

Pass Marks 50%

Theory Hours 100

Practical Hours : 200

Total study Hours: 300

Contents Of The Course

1. Introduction of Statistics: Statistical data condensation of data, presentation of

data by graphs, health related data, rates and their relative importance,

presentation of quantitative data.

2. Sampling: The concept of sampling, types and methods of drawing ideal

sample, sampling distribution of sample mean, error of sampling, standard error,

chi square, T-test and their uses in health.

3. Central Tendency: Concepts of central tendency, mean, median and ode and

their value in health, percentiles, measure of dispersion, coefficient of variation

and skewness, normal distribution, range, standard deviation and relative

deviation.

4. Hypothesis: Concepts of hypothesis testing, null & alternative hypothesis, two

types of errors, acceptance & rejection regions, tow sided & one sided tests,

general steps in hypothesis testing, test about means, confidence interval for

mean, meaning of significance in statistical procedures and methods of

inferential statistics.

5. Regression & Correlation: Scatter diagram, straight line regression model,

method of least squares, sample correlation coefficient, inference about

regression coefficient and correlation coefficient.

6. Introduction to Research: The question of legitimate knowledge, knowledge

& decision making, the scientific method, quantitative vs qualitative research,

application of scientific method, positivistic vs naturalistic paradigm.

7. Classification of Research: Basic vs applied research, evaluation research,

research & development (R&D), action research.

8. Selection & Formulation of a Problem: From generic to a specific program,

program statement, getting an access to primary and secondary resources, note

70

taking and information to management, Review of related literature, questions

and/or hypothesis of the study.

9. Development of a Research Plan: The ethical, legal and professional

obligations, the rational of the study, the research plan, evaluation of a research

plan.

10. Selection of sample: sample & population, basic considerations in sampling,

random sampling, stratified random sampling cluster sampling, systematic

sampling determination of sample size, elimination of sampling bias.

11. Instrumentation and Data Collection: Tests and scales, objectivity and

standardization, types of tests and scales, validity and reliability of an

instrument, assessment of validity and reliability, development of tests/scale.

12. Data Analysis & Interpretation: Preparing data analysis, types of

measurement scales, descriptive statistics inferential statistics, using computer

for data analysis.

13. Preparation of a Research Report: Format & style, citation, references &

bibliography writing theses, dissertations & journal articles.

Student will prepare a research report regarding the selected elective

subject under the guidance of the approved supervisor for the elective

subject.

The research report will be submitted in the final year examination.

Paper II

ELECTIVE SUBJECT

Theory Marks 90


Practical Marks 180


Total Marks 200

Pass Marks 50%

Theory Hours 100

Practical Hours : 200

Total study Hours: 300

Student in final year will select one subject from the elective subjects mentioned below, in the

first month of final year.

Principal / Head of institution will approve the supervisor

Immunohematology (Blood Banking)

Clinical Microbiology

Advanced Clinical Immunology

Medical Genetics

Molecular Biology

Advanced Clinical Chemistry

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Advanced Clinical Chemistry Course Contents:

Endocrine tests:

1. Cortical hormone.

2. Sex hormone.

3. Protein bound iodine. 4. Thyroid hormones.

Tumour markers:

Definition, a feto protein, CEA, HCG, CA, PSA, CA125, etc.

Miscellaneous:

1. Cerebrospinal fluid (detailed examination)

2. Lipoprotein by Electrophoresis.

3. Types of haemoglobin..

4 Copper.

Special Diseases and Their Laboratory Diagnosis:

1. Diabetes Mellitus

2. Phenylketonuria

3. Aminoaciduria

4. Glycogen storage disease

5. Renal calculi.

6. Proteinuria

7. Ketonuria.

8. Myocardial infarction

9. Nephrotic syndrome

10. Malabsorption syndrome

11. Hyperbilirubinaemia & Jaundice

12. Hypoalbuminaemia

13. Hyperbilirubinaemia

14. HyiJeruricaemia

15. Obesity

16. Acid base disorder

17. Cushing disease

18. Myxedema

19. Hypo & Hyperpituitarism

20. Diabetes inspidus

21. Amenorrhea

22. Hirsutism

23. Rickets

24. Osteomalacia

25. Chronic renal failure

Quality Control and Quality Assurance

OGTT

Therapeutic Drug Monitoring and Chemical Toxicology

PCR (Methodology, Principle and Clinical Interpretation)

Accreditation.

Practical:

Research Assignments Related to Elective Subjects

Advanced Lab Training Related to Elective Subjects

72

RECOMMENDED BOOKS AND JOURNALS

Essential Hematology By Hoffbrand A.V And Pettit

Practical Heamtology By Dacie And Lewis

Clinical Pathology Interpretations by A.H. Nagi

Clinical Hematology In Medical Practice By De- Gruchy’s

Handbook of Hematology and Blood Transfusion Techniques by JW Delancy

Manual Of Laboratory Medicine by AFIP, Rwp.

District Laboratory Practice In Tropical Countries: Part I & II By Monica Cheesburgh

Medical Microbiology And Immunology By Levinson And Jawetz

Textbook Of Clinical Chemistry By Warley

A Manual Of Laboratory And Diagnostic Tests By Francis Talaska

Text Book Of Clinical Chemistrty by Zilva Pannel

Short Textbook Of Chemical pathology by Baron

Clinical Chemistry In Practical Medicine by Stewart And Dunlopp

Immunology By Roih, Brostoff And Male (6th

Edition)

Immunobiology (The Immune System In Health And Disease By Janeway, Travers,

Walport & Shlomohick (6th

Edition)

Immunology By Abbas

Fundamental Immunology (5th

Edition) By William E Paul

Practical Medical Microbiology by Sherrys

Guide To Human Parasitology by Black Lock

Medical Bacteriology: A Practical Approach by Peter Hawky

Basic Medical Lab Technology by CJ Kirk and RN Peel

Theory and Practice Of Histololgical Techniques by John D Bancroft

Educational Research: Competencies For Analysis And Applications Columbus: Merrill.

Gay. L.R. (1987) Walpole, R.E.:

Introduction To Statistic, Publishing Co. Inc, New York.

Spiegel, Murray R.: Theory & Problems Of Statistics, Sehawm Publishing Co., New York.

Park’s; Text Book Of Preventive And Social Medicine

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