The comparing of natural antibacterial substance on E. Coli

Manhasset Science ResearchJean Cheng Period 4

http://www.scielo.br/img/revistas/bjm/v39n4/arq27_fig01.jpg

Figure 1: Antimicrobial drugs resistance pattern of urinary E. coli from Southern Brazil

Need

Figure 2: Chemical formula for Penicillinhttp://itech.dickinson.edu/chemistry/wp-content/uploads/2008/04/penicillin-core1.png

Knowledge Base

Figure 2: Chemical formula for Penicillinhttp://itech.dickinson.edu/chemistry/wp-content/uploads/2008/04/penicillin-core1.png

Knowledge Base

Figure3: Penicillin attacking the peptidocglycan http://upload.wikimedia.org/wikipedia/commons/thumb/8/8b/Gram_negative_cell_wall.svg/800px-Gram_negative_cell_wall.svg.png

Knowledge Base (cont)

Peptidoglycan

Knowledge Base (cont)

Figure3: Penicillin attacking the peptidocglycan http://upload.wikimedia.org/wikipedia/commons/thumb/8/8b/Gram_negative_cell_wall.svg/800px-Gram_negative_cell_wall.svg.png

Peptidoglycan

Figure 4: Tea tree’s terpene chemical formula

http://www.wacker.com/cms/appL/mdm_images/R67_IV-1_-432011277_8959.jpg

Figure 5: Pectin Chemical formula

http://sci-toys.com/ingredients/pectin_2.gif

Knowledge Base (cont)

Figure 6: Aloe verahttp://apps.who.int/medicinedocs/fr/d/Js2200e/5.html

Knowledge Base (cont)

Knowledge Base (cont)

Figure 7: Citralhttp://www.impgc.com/images/Structures/Citral.bmp

Figure 8:Chemical Change in Allicin subjected to different conditions. (Ariga, et al. 2008)

http://media-2.web.britannica.com/eb-media/33/7433-004-96DFD197.gif

Literature Review

Figure 9: Antimicrobial activity of aloe vera gel

(Alemdar, et al. 2009)

Literature Review (cont)

Figure 10:Yuzu fruit seed oil on different microbial

(Nakazono, et al. 2006)

Literature Review (cont)

Figure 11:12 different essential oil on different species of bacteria.

(Rusenova, et al. 2009)

Literature Review (cont)

Find which natural substance is best in inhibiting E. coli.

Null Hypothesis: No effectAlternative Hypothesis: Tea tree oil is the

best antimicrobial substance against E. coli

Purpose

The comparing of natural antibacterial substance on E. Coli

Essential oil and antibiotic plant substanceN=15

Garlic (allicin)N=3

Tea Tree oilN=3

Petri dish, E.coli, filter paper, glass spreader

Disc diffusion assay method

SPSS ANOVA Test

Aloe (anthraquinones)N=3

Yuzu fruit oilN=3

Millimeter

Methodology

Ginger (citral)N=3

Bacteria on agar

Inhibited Zone(mm)

Paper disk with substance being tested

Figure 12: Disk Diffusion method

http://diverge.hunter.cuny.edu/~weigang/Images/20-17_diskdiffusion.jpg

Protocols

Figure 13: http://gsbs.utmb.edu/microbook/images/fig10_5.jpg

Protocols (cont)

Not expensivedoesn’t require large amount of spaceSome materials found in lab or

supermarketE. coli is needed as well as agar, aloe, tea

tree, yuzu, and garlicNatural antiseptic doesn’t have negative

effect

Do-Ability

Vendor Cat# pg Item Qty Unit$ Total$Carolina 124400 Escherichia coli, Living, 5 mL 1 $9.20 $9.20Carolina 821040 Tryptic Soy Agar Media Kit 1 $26.95 $26.95Carolina 703413 Glass Spreader 1 $4.70 $4.70Ward 86 V 5900 Aloe Living Specimen 1 $7.95 $7.95Vitamin Shoppe Australian Tea tree Oil 1 7.99 $7.99

$56.79

Budget

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