Supplementary figure 3
1
r a S I R P R I K Q K K A K G S T S S T R L H E P E K N A R E I T Q I Q D T N D I N D I T Y A D L N L P K E K K P A P R V P E P N N H T E Y A S I E T G K L P R P E D T L T Y A D L D M V H L N R - - A Q P T P K P E P S F S E Y A S V Q V Q R K 5 0 9 m u S I R P R I K Q K K A K G S T S S T R L H E P E K N A R E I T Q I Q D T N D I N D I T Y A D L N L P K E K K P A P R A P E P N N H T E Y A S I E T G K V P R P E D T L T Y A D L D M V H L N R - - A Q P A P K P E P S F S E Y A S V Q V Q R K 5 0 9 h u S I R P 1 R I R Q K K A Q G S T S S T R L H E P E K N A R E I T - - Q D T - - - N D I T Y A D L N L P K G K K P A P Q A A E P N N H T E Y A S I Q T S P Q P A S E D T L T Y A D L D M V H L N R T P K Q P A P K P E P S F S E Y A S V Q V P R K 5 0 3 * * : * * * * : * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * : . . * * * * * * * * * * * : * . * . * * * * * * * * * * * * * * * * * * : * * * * * * * * * * * * * * * * * S u p p l e m e n t a r y f i g u r e 3 S u p p l e m e n t a r y F i g u r e 3 . A l i g n m e n t o f t h e c y t o p l a s m i c S I R P p r o t e i n s e q u e n c e s . A l i g n m e n t s w e r e p e r f o r m e d u s i n g t h e C l u s t a l W a l g o r i t h m ( v e r s i o n 1 . 8 2 ) e m p l o y i n g t h e f o l l o w i n g s e q u e n c e s : r a t S H P S - 1 ( g i _ 6 6 7 1 6 4 0 ) , m o u s e S H P S - 1 ( g i _ 1 7 7 7 3 5 4 ) , a n d h u m a n S I R P 1 ( g i _ 2 0 5 2 0 5 6 ) . T h e t w o p r o l i n e - r i c h r e g i o n s a r e o v e r l i n e d a n d t h e t y r o s i n e a n d p r o l i n e r e s i d u e s a r e h i g h l i g h t e d i n b l u e a n d r e d , r e s p e c t i v e l y . T h e p r o l i n e r e s i d u e s t h a t , a t l e a s t i n c a s e o f t h e h u m a n S I R P 1 , a r e d i s p e n s a b l e f o r J A K 2 b i n d i n g ( 4 2 ) a r e i n d i c a t e d b y a r r o w s .
description
raSIRP RIKQKKAKGSTSSTRLHE P EKNAREITQIQDTNDINDIT Y ADLNL P KEKK P A P RV P E P NNHTE Y ASIETGKL P R P EDTLT Y ADLDMVHLNR--AQ P T P K P E P SFSE Y ASVQVQRK 509 - PowerPoint PPT Presentation
Transcript of Supplementary figure 3
raSIRP
RIKQKKAKGSTSSTRLHEPEKNAREITQIQDTNDINDITYADLNLPKEKKPAPRVPEPNNHTEYASIETGKLPRPEDTLTYADLDMVHLNR--AQPTPKPEPSFSEYASVQVQRK 509
muSIRP
RIKQKKAKGSTSSTRLHEPEKNAREITQIQDTNDINDITYADLNLPKEKKPAPRAPEPNNHTEYASIETGKVPRPEDTLTYADLDMVHLNR--AQPAPKPEPSFSEYASVQVQRK 509
huSIRP
1 RIRQKKAQGSTSSTRLHEPEKNAREIT--QDT---NDITYADLNLPKGKKPAPQAAEPNNHTEYASIQTSPQPASEDTLTYADLDMVHLNRTPKQPAPKPEPSFSEYASVQVPRK 503
**:****:******************* *** ************ *****:..***********:*. * .**************** **:*************** **
Supplem
entary figure 3
Supplem
entary Figure 3.
Alignm
ent of the cytoplasmic S
IRP
protein sequences. Alignm
ents were perform
ed using the ClustalW
algorithm (version 1.82)
employing the follow
ing sequences: rat SH
PS
-1 (gi_6671640), mouse S
HP
S-1 (gi_1777354), and hum
an SIR
P
1 (gi_2052056). The
two proline-rich regions are overlined and the tyrosine and proline residues are highlighted in blue and red, respectively. T
he proline residues that, at least in case of the hum
an SIR
P
1, are dispensable for JAK
2 binding (42) are indicated by arrows.
