Supplementary Figure 1

13
Supplementary Figure 1 PIG 3 cata lase MW(kDa) 72 55 43 34 p 53 c atal ase MW(kDa) 72 55 43 34 26 95 130 170 A B

TAGS:

description

catalase. p53. MW(kDa). 170. 130. catalase. 95. PIG3. MW(kDa). 72. 55. 72. 43. 55. 34. 43. 34. 26. Supplementary Figure 1. A. B. Vector PIG3. . 160. . . 140. 120. 100. DCF fluorescence level. 80. 60. 40. 20. 0. H1299. SW480. HCT116 p53 -/-. - PowerPoint PPT Presentation

Transcript of Supplementary Figure 1

Page 1: Supplementary Figure 1

Supplementary Figure 1

PIG3

cata

lase

MW(kDa)

72

55

43

34

p53

cata

lase

MW(kDa)

7255

43

34

26

95130170

AA BB

Page 2: Supplementary Figure 1

-tubulin

catalase

PIG3

VectorPIG3

Cat

alas

e ac

tivi

ty (

U/m

l)

0.0

0.2

0.4

0.6

0.8

1.0

1.2

1.4

H1299 HCT116 p53-/-SW480

AA

DC

F f

luor

esce

nce

leve

l

VectorPIG3

H1299

HCT116 p53-/-

SW480

0

20

40

60

80

100

120

140

160

BB

Supplementary Figure 2

Page 3: Supplementary Figure 1

HCT116

siRNA

U2OS RKO

Control p53

p53

p53R2

SESN1

SESN2

GPX1

ALDH4A1

GAPDH

catalase

SOD2

TIGAR

Control p53 Control p53

TP53INP1

Nrf-2

Supplementary Figure 3

Page 4: Supplementary Figure 1

catalase

p53

0 4 8 12 24 0 4 8 12 24 (hr)

HCT116 p53-/-

UV (10 J/m2)

-tubulin

HCT116

p53R2

0 0.05 0.1 1 2 0 0.05 0.1 1 2

HCT116 p53-/-

H2O2 (mM)

HCT116

UV (10 J/m2) H2O2 (mM)

0 0.05 0.1 1 2 0 0.05 0.1 1 2

Saos-2 U2OS

catalase

p53

0 4 8 12 24 0 4 8 12 24 (hr)

Saos-2

-tubulin

U2OS

p53R2

Supplementary Figure 4

Page 5: Supplementary Figure 1

MW(kDa)

72

55

43ca

tala

se

p53

R2

Supplementary Figure 5

Page 6: Supplementary Figure 1

Cat

alas

e ac

tivi

ty (

%)

0

50

100

150

200

250

vector wt R2 R4

n.s.

AA

RO

S le

vels

(%

)

0

20

40

60

80

100

120

vector wt R2 R4

n.s.

Supplementary Figure 6

BB

Page 7: Supplementary Figure 1

AABB

HCT116U2OS RKO

p53R2

catalase

-tubulin

Cat

alas

e ac

tivi

ty (

U/m

l)

0.0

0.2

0.4

0.6

0.8

1.0

1.2

1.4

1.6

1.8

n.s.

Control siRNAp53R2 siRNA

HCT116 U2OS RKO0

20

40

60

80

100

120

140

160

n.s.

Control siRNAp53R2 siRNA

Supplementary Figure 7

RO

S le

vels

(%

)

Page 8: Supplementary Figure 1

p53R2

catalase

-tubulin

Cat

alas

e ac

tivi

ty (

U/m

l)

HCT116U2OS RKO

AA BB

0.0

0.5

1.0

1.5

2.0

2.5

0

20

40

60

80

100

120

HCT116U2OS RKO

n.s.

n.s.

n.s.

n.s.

n.s.

n.s.

Supplementary Figure 8

Control vectorWild-type p53R2 Mutant p53R2 (V115L)

RO

S le

vels

(%

)

Control vectorWild-type p53R2 Mutant p53R2 (V115L)

Page 9: Supplementary Figure 1

p53R2

PIG3

Catalase

p53

β-actin

Sup P

UT

Sup P

PMP70

UV (10 J/m2)

Supplementary Figure 9

Page 10: Supplementary Figure 1

UT

catalase merge DAPI

p53

p53R2

p53

p53R2

Supplementary Figure 10

UV (10 J/m2)

Page 11: Supplementary Figure 1

0

20

40

60

80

100

120

140

160

180

200

0 8 24 h

Supplementary Figure 11

AA BB

0

5

10

15

20

25

30

35

0 24 48 h

Ap

opto

sis

(%)

UV (40 J/m2)

U2OS (vector)U2OS (catalase)

UV (40 J/m2)

RO

S le

vels

(%

)

U2OS (vector)U2OS (catalase)

Page 12: Supplementary Figure 1

0 48 hAd p53

0

20

40

60

80

100

120

140

160

180

H1299 (vector)H1299 (catalase)

Supplementary Figure 12

RO

S le

vels

(%

)

Page 13: Supplementary Figure 1

Untreated controlUV (20 J/m2)

HCT116 p53-/-

Vector PIG3

0

20

40

60

80

100

120

140

160

180

200

Supplementary Figure 13

RO

S le

vels

(%

)


Supplementary Figure 1 - Welcome to the UCLA …€¦ ·  · 2016-06-151 Supplementary Figure 1 Supplementary Figure 1. ... Supplementary Figure 5 Supplementary Figure 5. ... JP10089

Supplementary Figure 1 - Welcome to the UCLA …€¦ ·  · 2016-06-151 Supplementary Figure 1 Supplementary Figure 1. ... Supplementary Figure 5 Supplementary Figure 5. ... JP10089

Supplementary Figure 1.

Supplementary Figure 1.

media.nature.com · 1 Supplementary Materials Supplementary Table S1 Supplementary Table S2 Supplementary Table S3 Supplementary Figure S1 Supplementary Figure S2 Supplementary Figure

media.nature.com · 1 Supplementary Materials Supplementary Table S1 Supplementary Table S2 Supplementary Table S3 Supplementary Figure S1 Supplementary Figure S2 Supplementary Figure

Supplementary Figures: Supplementary Figure 1 – PANCAN ...df6sxcketz7bb.cloudfront.net/manuscripts/88000/88755/jci.insight.88755.sd.pdfSupplementary Figures: Supplementary Figure

Supplementary Figures: Supplementary Figure 1 – PANCAN ...df6sxcketz7bb.cloudfront.net/manuscripts/88000/88755/jci.insight.88755.sd.pdfSupplementary Figures: Supplementary Figure

Supplementary Figure 1

Supplementary Figure 1

Supplementary Figure 1€¦ · Supplementary Figure 1 Supplementary Figure 1. Quality control (QC) steps for the selection of Htr3a-GFP+ cells.Single cell RNA-seq was performed on

Supplementary Figure 1€¦ · Supplementary Figure 1 Supplementary Figure 1. Quality control (QC) steps for the selection of Htr3a-GFP+ cells.Single cell RNA-seq was performed on

Supplementary information for: Discovery and … · Supplementary Figure S2 Supplementary Figure S3 Supplementary Figure S4 Supplementary Figure S5 Supplementary Figure S6 Supplementary

Supplementary information for: Discovery and … · Supplementary Figure S2 Supplementary Figure S3 Supplementary Figure S4 Supplementary Figure S5 Supplementary Figure S6 Supplementary

Supplementary Figure 1. Supplementary Figure 2 Supplementary Figure 3 A B.

Supplementary Figure 1. Supplementary Figure 2 Supplementary Figure 3 A B.

Supplementary figure 1

Supplementary figure 1

Supplementary Figure 1

Supplementary Figure 1

Supplementary figure 1

Supplementary figure 1

Supplementary Figure 1: Dependence of hysteresis in IV ...10.1038/s41467-017... · Supplementary Figure 1 . Supplementary Figure 1: Dependence of hysteresis in IV measurements upon

Supplementary Figure 1: Dependence of hysteresis in IV ...10.1038/s41467-017... · Supplementary Figure 1 . Supplementary Figure 1: Dependence of hysteresis in IV measurements upon

Supplementary Figure 1 - Nature · Supplementary Figure 1 ... Supplementary Figure 3. Activation of HIF-1 activity by UCHL1 overexpression. ... EMT6/EF-Luc/EV-1, 2 and

Supplementary Figure 1 - Nature · Supplementary Figure 1 ... Supplementary Figure 3. Activation of HIF-1 activity by UCHL1 overexpression. ... EMT6/EF-Luc/EV-1, 2 and

images.nature.com · Web viewSupplementary Figure 7 Supplementary Figure 3 Supplementary Figure 1 Supplementary Materials Supplementary Figures S1-S7 Supplementary Tables S1-S7 Clinical

images.nature.com · Web viewSupplementary Figure 7 Supplementary Figure 3 Supplementary Figure 1 Supplementary Materials Supplementary Figures S1-S7 Supplementary Tables S1-S7 Clinical

LEGENDS TO SUPPLEMENTARY FIGURES Supplementary Figure 1 ...

LEGENDS TO SUPPLEMENTARY FIGURES Supplementary Figure 1 ...

Ahmed Supplementary Figure 1

Ahmed Supplementary Figure 1