Supplementary figure 1

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Supplementary figure 1 Surface marker expression in % 0 13 17 28 0 20 40 60 80 100 120 CD31 CD34 VEGFR2 days A B Supplementary figure 1: Characterization of dmECFC and hECFC. ECFC clones showed a typical morphology. From left to right: 10x healthy, 10x dmECFCECFC, 40x magnification dmECFC (A). Expression of endothelial and progenitor markers in dmECFC and hECFC (n=3, B). Expression of CD31, CD34, and VEGFR2 was determined by flow cytometry over time (n=4- 6/time point; C). CD31 CD34 VEGFR2 0 20 40 60 80 100 120 hECFC dmECFC C Surface marker expression in % hECFC dmECFC

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Supplementary figure 1. A. hECFC. dmECFC. B. Surface marker expression in %. C. Surface marker expression in %. days. - PowerPoint PPT Presentation

Transcript of Supplementary figure 1

Page 1: Supplementary figure 1

Supplementary figure 1S

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Supplementary figure 1: Characterization of dmECFC and hECFC. ECFC clones showed a typical morphology. From left to right: 10x healthy, 10x dmECFCECFC, 40x magnification dmECFC (A). Expression of endothelial and progenitor markers in dmECFC and hECFC (n=3, B). Expression of CD31, CD34, and VEGFR2 was determined by flow cytometry over time (n=4-6/time point; C).

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Supplementary figure 2: mRNA expression of the specific adiponectin receptors AdipoR1 and AdipoR2 in diabetic and non-diabetic ECFC. RT-PCR for yECFC and dmECFC using hypoxanthine-guanine phosphoribosyl transferase (HPRT) as loading control (A), real-time PCR for age-matched hECFC and dmECFC using 18 S rRNA as house keeping gene (B).

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Supplementary figure 3: Concentration of nitrite as a quantitative measure of nitric oxide production in cell culture supernatants, *: p<0.05 vs. control, n=3.

Supplementary figure 3


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