Supplemental Figure 1

2
Supplemental Figure 1 t = 0 min t = 5 min t = 10 min t = 15 min t = 20 min B. Axonal Blebbing A. Growth Cone Ruffling t = 0 min t = 5 min t = 10 min t = 15 min t = 20 min 0 20 40 60 80 100 0 0 .5 1 1.5 2 2 .5 3 3 .5 Percent A verag e P o sitive V elo city (µ m /m in) N o Incubation 4 H our Incubation D. Average Positive Particle Velocity Histogram C. WGA Kymograph (4 hr incubation) Position Time Cell Body Growth Cone 0 min 20 min E. Confocal Imaging of WGA WGA *

description

B. Axonal Blebbing. t = 0 min. t = 0 min. t = 5 min. t = 5 min. t = 10 min. t = 10 min. t = 15 min. t = 15 min. t = 20 min. t = 20 min. A. Growth Cone Ruffling. D.Average Positive Particle Velocity Histogram. C. WGA Kymograph (4 hr incubation). 0 min. Time. *. 20 min. - PowerPoint PPT Presentation

Transcript of Supplemental Figure 1

Page 1: Supplemental Figure 1

Supplemental Figure 1

t = 0 min

t = 5 min

t = 10 min

t = 15 min

t = 20 min

B. Axonal Blebbing A. Growth Cone Ruffling

t = 0 min

t = 5 min

t = 10 min

t = 15 min

t = 20 min

0

20

40

60

80

100

0 0.5 1 1.5 2 2.5 3 3.5

Perc

en

t

Average Positive Velocity (µm/ min)

No Incubation

4 Hour I ncubation

D. Average Positive Particle Velocity Histogram

C. WGA Kymograph (4 hr incubation)

Position

Tim

e

Cell Body Growth Cone

0 min

20 min

E. Confocal Imaging of WGA

WGA

*

Page 2: Supplemental Figure 1

A. Average Positive Particle Velocity Histogram

B. Average Positive Particle Velocity Histogram (within 30 µm of the edge)

D. Average Negative Particle Velocity Histogram (within 30 µm of the edge)

C. Average Negative Particle Velocity Histogram

0

20

40

60

80

100

120

0 0.5 1 1.5

Perc

en

t

Average Positive Velocity (μm/ min)

NC

NNC

SCC

SCNC

0

20

40

60

80

100

120

0 0.5 1 1.5

Perc

en

t

Average Positive Velocity (μm/ min)

NC

NNC

SCC

SCNC

0

20

40

60

80

100

-2 -1.5 -1 -0.5 0

Perc

en

t

Average Positive Velocity (μm/ min)

NC

NNC

SCC

SCNC

0

20

40

60

80

100

-2 -1.5 -1 -0.5 0

Perc

en

t

Average Positive Velocity (μm/ min)

NC

NNC

SCC

SCNC

Supplemental Figure 2