Supplement 5

Comparison of homoeologous Kanota x Ogle (KO) linkage groups 24_26_34 and 22_44_18 and the location of As-Vrn1 mapped as an RFLP in KO. Stacked horizontal lines show the locations of additional markers placed on the map framework. Solid lines are used to join markers found in common between homologous oat groups, while dotted lines join markers found in common between homoeologous oat groups or chromosomes from different species. A list of markers joined by lines is provided separately. Boxes show the approximate locations of QTL identified in previous studies, some of which are placed on the KO map using comparative mapping (Wight et al. 2006). The letters in each box indicate which reference should be consulted for further information concerning the particular QTL: H, Holland et al. 1997; h, Holland et al. 2002; S, Siripoonwiwat et al. 1996. The letters in bold text in grey boxes indicate QTLs for which the experiment-wide type I error rate was p <0.05 or the comparison-wide type I error rate was p <0.001. The remaining QTL had comparison-wide type I error rates of p <0.05. The framework marker on KO group 24_26_34 corresponding to the regions on the U86 and U71P maps containing QTLs for flowering time and vernalization response is highlighted and boxed, as is the location of the Vrn1 gene on group KO 22. Other groups and highlighted markers provide comparative mapping evidence for the location of Vrn1 in this region. The OT (Ogle x TAM O-301) hexaploid oat map is from Portyanko et al. (2001), the fragment of the barley SM (‘Steptoe’ x ‘Morex’) chromosome 5H map is from Kleinhofs et al. 1993, and the simplified diagram of the fine mapping of the Vrn1 region of wheat chromosome 5Am is based on the work of Yan et al. (2003). The location of the Vrn-H1 gene on the barley map presented has been determined using the Graingenes database CMap comparative mapping function (http://wheat.pw.usda.gov/cmap/) and the results of von Zitzewitz et al. (2005).

(See figure on next page)

KO group 24_26_34

[0] umn5245_3

[6] opt-4800

[20] opt-2130_c[22] opt-8386_c

[32] opt-7581

[36] opt-18063

[41] opt-9567

[52] opt-12104

[57] cdo590a[61] cdo795a[64] opt-13764[68] opt-8018_c[70] opt-13924_c[74] opt-14251_c

[83] opt-5986

[93] bcd961

S

H

S

H H h h h

S H H H H

h h h

H

h

H

h

h

S

HS

e40m48-173.t [39]

OT group 1

cdo1081a [0]

cisu128b [33]

cdo87 [43]isu1877b [44]

rz912 [46]cisu92 [50]

cdo590a [57]rz474a [59]

cdo795a [65]isu1093 [68]psr153 [70]psr144 [71]adh2a [72]

cisu59a [73]

cdo504

isu1961a

BarleySM chromosome 5H

mwg851b [0]mwg851c [3]abg314b [6]abg463 [11]cdo484 [14]

abg390 [21]abg391 [24]

abc482 [30]

abc155 [37]

abg495a [44]mwg877 [47]

wg908 [51]iest9 [53]

cdo504 [58]

mwg514b [64]

wg644

isu1961brv

wg644

cdo708

Vrn1

Wheat chromosome 5A

m

0.1

0.9

KO group 22_44_18

opt-9086_c [0]cdo482b [2]

umn5596_1 [26]

umn5004 [62]isu1507 [67]

opt-9724 [73]opt-13454 [74]

opt-7417_c [76]opt-8196 [79]

opt-18069 [89]

cdo484a [94]

opt-1641_c [104]opt-17202 [107]opt-6646 [111]opt-5664 [112]

isu1961crv [114]opt-0526 [114]cdo708b [119]aco168a [128]

cdo608drv [133]

opt-15453 [142]opt-8693 [143]opt-5272 [144]

opt-11625_c [146]opt-9929_c [148]opt-14108 [151]opt-6715 [155]cdo1196 [158]bcd327 [167]

csu25 [178]

Vrn1

(Vrn-H1)

•See details of connecting lines on following page

KO_24_26_34 KO_22_44_18 OT_1 SM_5H wheat_5Am

opt-15408 opt-15408_c

umn6003b umn6003a

umn6003b umn6003c

cdo1469cdi cdo1469adi

cdo1523a cdo1523b

cdo241 cdo241a

cdo419b cdo419e

cdo484b cdo484a cdo484

cdo419b cdo419

cdo1437a cdo1437b

umn5207b umn5207a

bcd1797d bcd1797a

bcd1968b bcd1968c

cdo1505a cdo1505b

bcd1734 bcd1734

isu1877 isu1877b

wg644 wg644

cdo504 cdo504

Vrn1 Vrn1

isu1961brv isu1961crv isu1961a

cdo708b cdo708

bcd1643a bcd1643c

aco168b aco168a

umn589a umn589b

cdo590a cdo590c cdo590a

cdo795a cdo795b cdo795a

umn363a umn363b

cdo419f cdo419e

cdo419f cdo419

Details of map joins represented by connecting lines: