Thermostability enhancement of Luciola mingrelica firefly luciferase by in vivo directed evolution
STOPhersentumoren Symposium · STOPhersentumoren Symposium. Preclinical brain tumor models. Primary...
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Tom Wurdinger Cancer Center Amsterdam VU University Medical School STOPhersentumoren Symposium
Transcript of STOPhersentumoren Symposium · STOPhersentumoren Symposium. Preclinical brain tumor models. Primary...
Tom Wurdinger
Cancer Center Amsterdam
VU University Medical School
STOPhersentumoren Symposium
Preclinical brain tumor models
Primary glioma cells expressing Firefly
luciferase and fluorescent mCherry
Firefly luciferase / mCherry
Preclinical glioma models
Preclinical glioma models
WEE1 inhibitor: PD0166285
Control AZD1775 IR IR + AZD1775
AZD1775
Phase I/II trial
NCT01849146
WEE1 inhibitor: AZD1775 (NCT01849146)
MPS1 inhibitor: preclinical drug discovery
U87R1 tumor volumeU87 tumor volume U87 survival U87R1 survival
TMZ sensitizers: Hydroxyurea
2000-compound FDA drug screen
Hydroxyurea
Phase I/II trial
scheduled
CTRL
TMZ
RO
TMZ+RO
Day 6 Day 10 Day 13 Day 16 RLU
Click # LH2013061013502510 Jun 2013 13:50:38Bin:M (4), FOV12.5, f1, 1mFilter: OpenCamera: IVIS 11215, DW434
Series: cage 247 m1,2,3Experiment: Hs683 FMLabel: Comment: Analysis Comment:
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Color BarMin = 50000Max = 1e+06
bkg subflat-fieldedcosmicClick # LH20130614165739
14 Jun 2013 16:57:52Bin:M (4), FOV12.5, f1, 1mFilter: OpenCamera: IVIS 11215, DW434
Series: cage 247 m1,2,3Experiment: Hs683 FMLabel: Comment: Analysis Comment:
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Color BarMin = 50000Max = 1e+06
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Click # LH2013061710200617 Jun 2013 10:20:19Bin:M (4), FOV12.5, f1, 1mFilter: OpenCamera: IVIS 11215, DW434
Series: cage 247 m1,2,3Experiment: Hs683 FMLabel: Comment: Analysis Comment:
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Color BarMin = 50000Max = 1e+06
bkg subflat-fieldedcosmic
Click # LH2013062016081620 Jun 2013 16:08:27Bin:M (4), FOV12.5, f1, 1mFilter: OpenCamera: IVIS 11215, DW434
Series: cage 259 m1,2,3Experiment: Hs683 FMLabel: Comment: Analysis Comment:
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Color BarMin = 50000Max = 1e+06
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Click # LH2013061013340410 Jun 2013 13:34:18Bin:M (4), FOV12.5, f1, 1mFilter: OpenCamera: IVIS 11215, DW434
Series: cage 246 m1,2,3Experiment: Hs683 FMLabel: Comment: Analysis Comment:
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Color BarMin = 50000Max = 1e+06
bkg subflat-fieldedcosmicClick # LH20130614163904
14 Jun 2013 16:39:17Bin:M (4), FOV12.5, f1, 1mFilter: OpenCamera: IVIS 11215, DW434
Series: cage 246 m1,2,3Experiment: Hs683 FMLabel: Comment: Analysis Comment:
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Color BarMin = 50000Max = 1e+06
bkg subflat-fieldedcosmic
Click # LH2013061710040917 Jun 2013 10:04:22Bin:M (4), FOV12.5, f1, 1mFilter: OpenCamera: IVIS 11215, DW434
Series: cage 246 m1,2,3Experiment: Hs683 FMLabel: Comment: Analysis Comment:
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910
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Color BarMin = 50000Max = 1e+06
bkg subflat-fieldedcosmic
Click # LH2013062016243520 Jun 2013 16:24:48Bin:M (4), FOV12.5, f1, 1mFilter: OpenCamera: IVIS 11215, DW434
Series: cage 260 m1,2,3Experiment: Hs683 FMLabel: Comment: Analysis Comment:
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Color BarMin = 50000Max = 1e+06
bkg subflat-fieldedcosmic
Click # LH2013061013260210 Jun 2013 13:26:16Bin:M (4), FOV12.5, f1, 1mFilter: OpenCamera: IVIS 11215, DW434
Series: cage 245 m4,5,6Experiment: Hs683 FMLabel: Comment: Analysis Comment:
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Color BarMin = 50000Max = 1e+06
bkg subflat-fieldedcosmic
Click # LH2013061416301514 Jun 2013 16:30:29Bin:M (4), FOV12.5, f1, 1mFilter: OpenCamera: IVIS 11215, DW434
Series: cage 245 m4,5,6Experiment: Hs683 FMLabel: Comment: Analysis Comment:
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Color BarMin = 50000Max = 1e+06
bkg subflat-fieldedcosmic
Click # LH2013061709551817 Jun 2013 09:55:31Bin:M (4), FOV12.5, f1, 1mFilter: OpenCamera: IVIS 11215, DW434
Series: cage 245 m4,5Experiment: Hs683 FMLabel: Comment: Analysis Comment:
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Color BarMin = 50000Max = 1e+06
bkg subflat-fieldedcosmic
Click # LH2013061909360319 Jun 2013 09:36:16Bin:M (4), FOV12.5, f1, 1mFilter: OpenCamera: IVIS 11215, DW434
Series: cage 257 m3,4Experiment: Hs683 R1 FMLabel: Comment: Analysis Comment:
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Color BarMin = 50000Max = 1e+06
bkg subflat-fieldedcosmic
Click # LH2013061013071910 Jun 2013 13:07:32Bin:M (4), FOV12.5, f1, 1mFilter: OpenCamera: IVIS 11215, DW434
Series: cage 244 m4,5,6Experiment: Hs683 FMLabel: Comment: Analysis Comment:
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Color BarMin = 50000Max = 1e+06
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Click # LH2013061416113814 Jun 2013 16:11:51Bin:M (4), FOV12.5, f1, 1mFilter: OpenCamera: IVIS 11215, DW434
Series: cage 244 m4,5,6Experiment: Hs683 FMLabel: Comment: Analysis Comment:
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Color BarMin = 50000Max = 1e+06
bkg subflat-fieldedcosmic
Click # LH2013061709390317 Jun 2013 09:39:17Bin:M (4), FOV12.5, f1, 1mFilter: OpenCamera: IVIS 11215, DW434
Series: cage 244 m5,6Experiment: Hs683 FMLabel: Comment: Analysis Comment:
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Color BarMin = 50000Max = 1e+06
bkg subflat-fieldedcosmic
Click # LH2013062016001920 Jun 2013 16:00:32Bin:M (4), FOV12.5, f1, 1mFilter: OpenCamera: IVIS 11215, DW434
Series: cage 258 m5,6Experiment: Hs683 R1 FMLabel: Comment: Analysis Comment:
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Color BarMin = 50000Max = 1e+06
bkg subflat-fieldedcosmic
Click # LH2013061710200617 Jun 2013 10:20:19Bin:M (4), FOV12.5, f1, 1mFilter: OpenCamera: IVIS 11215, DW434
Series: cage 247 m1,2,3Experiment: Hs683 FMLabel: Comment: Analysis Comment:
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910
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Color BarMin = 50000Max = 1e+06
bkg subflat-fieldedcosmic
Phase I/II trial
ongoing
NCT01119599
TMZ sensitizer: RO4929097 (NCT01119599)
TAKE HOME MESSAGE: NEW THERAPIES ON THE HORIZON
Hydroxyurea
Phase I/II trial
Scheduled
RO4929097
Phase I/II trial
NCT01119599
MPS1i
Preclinical study
Ongoing
AZD1775
Phase I/II trial
NCT01849146
Rindopepimut NCT01480479
Valganciclovir (Valcyte; FDA approved)
Bevacizumab (Avastin; FDA approved)
BRAIN TUMOR CENTER AMSTERDAM
Neuro-oncology Research Group
VU University medical center | Cancer Center Amsterdam
Tom Wurdinger
Cancer Center Amsterdam
VU University Medical School
STOPhersentumoren Symposium
