SL210 Users Manual

An ISO 90001 / 14001 / 27001 Certified

DOUBLE BEAM UV-VIS SPECTROPHOTOMETER

SL 210

Users Manual

ELICO Limited

SAFETY PRECAUTIONS The following general safety precautions must be observed during all phases of operation, service and repair of this instrument. Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design, manufacture and intended use of the instrument. ELICO assumes no liability for the customers failure to comply with these requirements.

GROUND THE INSTRUMENT To minimize shock hazard, the instrument chassis and cabinet must be connected to an electrical ground. The instrument is equipped with a three conductor power cable. The power cable must either be plugged into an approved three-contact electrical socket or used with a three-contact to two-contact adapter with the grounding wire (green) firmly connected to an electrical ground (safety ground) at the power socket. Mating plug of the power cable should meet relevant specifications of Bureau of Indian Standards (BIS).

DO NOT OPERATE IN AN EXPLOSIVE ATMOSPHERE Do not operate the instrument in the presence of inflammable gases or fumes. Operation of any electrical instrument in such an environment constitutes a definite safety hazard.

KEEP AWAY FROM LIVE CIRCUITS Do not install substitute parts or perform any unauthorized modifications to the instrument. It may be introducing additional hazards. Return the instrument to the nearest ELICO sales and Support Centre for service and repair to ensure that safety features are maintained.

FOLLOW THESE SIGNS STRICTLY

NOTE : NOTE sign denotes important information. It calls attention to procedure, practice, condition etc., which is essential to highlight.

CAUTION : CAUTION sign denotes a hazard. It calls attention to an operating procedure, practice, condition etc., which if not correctly performed or adhered to, could result in damage or destruction of part or all of the product or an injury to personnel.

WARNING : Sign denotes, Hot surface. It calls attention to practice i.e., not to be touched. When the flame is ON

USERS MANUAL 232 - UM - 01

DOUBLE BEAM UV-VIS SPECTROPHOTOMETER

SL 210

APPLICABILITY

This manual applies to instrument With Serial Number ___________

NOTE

Keep with instrument

WARNING

To reduce risk of electrical fire or shock hazards, do not expose

this instrument to rain or excessive moisture.

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INDEX

SECTION CONTENTS PAGE

1 GENERAL INFORMATION 1-1 1.1 INTRODUCTION 1-1 1.2 PRINCIPLE OF OPERATION 1-1 1.3 SPECIFICATIONS 1-1 1.4 INSTRUMENT AND MANUAL IDENTIFICATION 1-2 1.5 RECOMMENDED SPARES 1-2 1.6 SAFETY CONSIDERATIONS 1-2

2 INSTALLATION 2-1

2.1 INTRODUCTION 2-1 2.2 INITIAL INSPECTION 2-1 2.3 INSTALLATION REQUIREMENTS 2-1 2.4 REPACKING FOR SHIPMENT 2-2

3 OPERATION 3-1

3.1 INTRODUCTION 3-1 3.2 DESCRIPTION 3-1 3.3 LAYOUT OF DISPLAY, CONTROLS, READOUTS Etc. ON

PANELS 3-1

3.4 PRE-OPERATING INSTRUCTIONS 3-4 4 OPERATIONAL FLOW 4-1

4.1 INITIALIZATION 4-1 4.2 SPECTRUM 4-2

Spectrum VIEW 4-3 Spectrum PRINT 4-3 - TABLE 4-4 - GRAPH 4-5 ABSORBANCE 4-5 % TRANSMISSION 4-5

4.3 PHOTOMETRIC 4-6 Photometric VIEW 4-7 Photometric PRINT 4-8

4.4 QUANTITATIVE 4-8 STANDARD METHOD 4-9 - SELECTION OF UNITS 4-9 - CALIBRATION 4-10 OPTION OLD/NEW CAL DATA 4-10 ABSORBANCE INPUT KEYING-IN THE VALUES OF CONCENTRATION OF

STANDARDS IN PPM 4-10

ABSORBANCE INPUT

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INDEX

SECTION CONTENTS PAGE KEYING-IN THE VALUES OF CONCENTRATION OF

STANDARDS IN MOLES 4-10

MEASURE 4-10 KEY IN 4-11 -FACTOR METHOD 4-12 - MEASUREMENT 4-12 VIEW - DATA OF SAMPLES 4-13 PRINT DATA OF SAMPLES 4-14 AUTOZERO 4-14 FILE 4-14 FILE VIEW 4-15 FILE PRINT 4-15 FILE LOAD 4-15 FILE DELETE 4-16

4.5 SYSTEM MODE 4-16 - LAMPS 4-16 -BASELINE 4-16 -DIAGNOSTICS 4-17

4.6 TIME SCAN 4-17 4.7 STANDARDS PRESENTATION 4-19

VIEW STANDARDS DATA 4-19 SAVE - STANDARDS DATA 4-20

4.8 SAMPLES PRESENTATION 4-21 4.9 MODES 4-21

5. CHECKING THE PERFORMANCE 5-1

5.1 PRE-PERFORMANCE CHECK INSTRUCTIONS 5-1 5.2 SPECTRAL CALIBRATION 5-1 5.3 PHOTOMETRIC PERFORMANCE 5-1

- ABSORBANCE 5-2 - % TRANSMISSION 5-2 - CONCENTRATION 5-2 6. TROUBLE SHOOTING 6-1 ANNEXURES A THEORY OF COLORIMETRY AND SPECTROSCOPY A-1 B PRINCIPLE OF OPERATION B-1 C SPECIFICATIONS OF SL 210 C-1 D DOUBLE BEAM UV-VIS SPECTROPHOTOMETER SL 210 D-1 E CARE & MAINTENANCE OF CELLS E-1 F ELECTROMAGNETIC SPECTRUM F-1

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1. GENERAL INFORMATION 1.1 INTRODUCTION The information contained in this manual is for installation and operation of

ELICOs MICROPROCESSOR (mP) BASED DOUBLE BEAM UV-VIS SPECTROPHOTOMETER, SL 210. This can also be operated with PC through its USB Interface with the help of ELICOs Windows based software viz., SpectratreatsT M (optional).

This section of the manual covers the principle of operation, theory of SPECTRO-

PHOTOMETRY, Specifications, Manual identification and other general information on the instrument.

1.2 PRINCIPLE OF OPERATION 1.2.1 DOUBLE BEAM UV-VIS SPECTROPHOTOMETER, SL 210 works on the

principle of SPECTROPHOTOMETRY based on Beer-Lambert Law. 1.2.2 SPECTROPHOTOMETRY

A brief theory of COLORIMETRY & SPECTROPHOTOMETRY is given at the end of the manual.

Annexure - A

1.2.3 PRINCIPLE

Monochromatic light passes through media of Reference and standard solutions of known concentration and the Sample-under-test in identical conditions. The transmitted light intensities through these media are detected, compared and processed to present

a. Absorbance and percentage transmission of the sample. b. Concentration of the sample in comparison to the solution of known Concentration at the wavelength selected

Annexure - B 1.3 SPECIFICATIONS Specifications of DOUBLE BEAM UV-VIS SPECTROPHOTOMETER, SL 210 are

given at the end of the manual.

Annexure - C

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1.4 INSTRUMENT AND MANUAL IDENTIFICATION The instrument is identified by a serial number on the number plate on the rear panel of the instrument. The manual applies to the instrument with serial number as indicated on the title page.

1.5 RECOMMENDED SPARES The following spares are recommended. Available at extra cost: - 3A 250V cartridge fuse for 90 to 260V AC supply voltage - 2 Nos. - Deuterium Lamp - 1 No. - Tungsten Lamp - 1 No.

CAUTION

Do not touch the glass envelopes of the lamps, particularly quartz window of Deuterium Lamp.

NOTE

After replacement of Deuterium & Tungsten Lamps optical alignment may be necessary. Contact your nearest ELICO Sales & Support Center.

1.6 SAFETY CONSIDERATIONS The users manual should be read and safety precautions fully understood before using

the instrument.

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2. INSTALLATION

2.1 INTRODUCTION This section of the manual contains necessary information and instructions to

install DOUBLE BEAM UV-VIS SPECTROPHOTOMETER, SL 210, including initial inspection procedures, power & grounding requirements and instructions to repack the instrument for shipment in the unlikely event of such a necessity arising.

2.2 INITIAL INSPECTION This instrument is carefully inspected both mechanically and electrically before

shipment. It should be free of mars, scratches and perfect electrically on receipt. The instrument should be inspected for any damage that may have occurred during transit. If the shipping container or packing material is damaged, it should be kept till the contents of the shipment have been checked as per packing list and instrument has been mechanically checked. Procedure for checking the performance of DOUBLE BEAM UV-VIS SPECTROPHOTOMETER, SL 210 is given in section 4. If shipping container is damaged, or contents are incomplete, or instrument does not function as per performance checks, notify your nearest ELICO Sales and Support Center. Preserve shipping materials for inspection.

2.3 INSTALLATION REQUIREMENTS 2.3.1 LOCATION The location where the DOUBLE BEAM UV-VIS SPECTROPHOTOMETER

SL 210 is proposed to be installed should be

- Free from Dust Corrosive gases Vibration & shocks - Sheltered from direct sun light - Away from intense EM & RF Interference - Well ventilated with ambient temperature between 15C & 35C and

Relative humidity between 45% to 80%. 2.3.2 PLACEMENT OF INSTRUMENT The instrument should be placed on a sturdy laboratory table with a flat rigid top.

The instrument requires a table space of 600 mm x 500 mm. A clearance of at least 250 mm is required on the sides and rear side of the instrument for proper ventilation and operational convenience.

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NOTE The clearance in the rear and on the fan-side of instrument is essential to ensure proper ventilation for the heat generating components inside.

The instrument is provided with soft, insulating & non-scratching feet and a

three-conductor power cord with a molded 3 pin 5A-which when plugged into an appropriate power outlet grounds the instrument (panel, cabinet and chassis).

NOTE

Ensure that correct fuse is installed in the fuse holder on the rear side of the instrument.

2.3.3 POWER REQUIREMENT

DOUBLE BEAM UV-VIS SPECTROPHOTOMETER SL 210 requires power supply of 90-260V, 50 Hz, single phase in 3 contact 5A AC line-filtered power outlets conforming to relevant specification of Bureau of Indian Standards (BIS), preferably with switch, fuse and indicator. Maximum power consumption of the instrument is 170 VA.

NOTE If Printer is proposed to be used provision for the printer and line-filtered power for it are also to be made by the user.

2.4 REPACKING FOR SHIPMENT

NOTE If the instrument is to be shipped to ELICO for service or repair, attach a tag to the instrument identifying the owner and indicating type number and serial number of the instrument and service or repair to be carried out. In case of repair, a detailed note on the defects observed must be sent along with the instrument.

Remove the 3 contact-molded plug of the instrument from the power outlet, and

also from the power receptacle of the instrument Place the instrument wrapped in heavy plastic sheet, in the original container,

with original packing material and secured as it was originally received. If the original container is not available, wrap the instrument in heavy plastic

sheet and pack in shipping container in a manner similar to that in which it was received.

Mark the shipping container DELICATE INSTRUMENT, FRAGILE etc.,

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3. OPERATION

3.1 INTRODUCTION This section of manual deals with the information, instructions for operation of

DOUBLE BEAM UV-VIS SPECTROPHOTOMETER SL 210 for measurement and analysis of Absorbance, Percentage transmission & Concentration of Samples.

3.2 DESCRIPTION 3.2.1 DOUBLE BEAM UV-VIS SPECTROPHOTOMETER, SL 210 is a table top,

ergonomically engineered instrument with its components and modules inside laid out for easy maintainability and fully automatic operation through the keyboard - the only controls available on its panels.

Annexure D

A selectable light beam in Ultra-violet or Visible spectrum from the LIGHT SOURCE module gets focused on to the entrance slit of MONOCHROMATOR module. The incident beam of light gets tuned to keyboard-settable wavelength by motorized slewing of the holographic grating in Czerny-Turner mount and reflecting optics and emerges as a monochromatic light of narrow band width from the exit slit of the Monochromator. This narrow band light from monochromator is switched between two paths by a rotating chopper - one for REFERENCE CELL and other for SAMPLE CELL. The transmitted portion of the light through the media (BLANK, STANDARD & SAMPLE SOLUTION) in the two paths is combined onto a single photo detector. The output of DETECTOR-PRE-AMPLIFIER module is processed in the ELECTRONICS module and the ABSORBANCE/PERCENTAGE TRANSMISSION/ CONCENTRATION of the SAMPLE medium is present in Scan, Photometric, and Concentration, Time Scan modes.

With self-check & self-diagnostic facility on LCD read out for monitoring and on printer for hard copy.

3.3 LAY OUT OF DISPLAY, CONTROLS, READOUTS Etc. ON PANELS 3.3.1 FRONT PANEL READOUT (1) READOUT - 20 x 4 DOT MATRIX ALPHA NUMERIC

LCD DISPLAY OF 2.95 x 4.75 SIZE CHARACTERS - To display prompts - for user friendly interaction, entered data and values of the measured outputs.

NUMBER KEYBOARD

(2) KEYBOARD - Input device through which different operations can be made. The function of each key is given below:

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ALPHANUMERIC KEYS 0/*() (2.1) 0/*( )

1/STU (2.2) 1/STU

2/VWX (2.3) 2/VWX

3/YZ (2.4) 3/YZ

4/JKL (2.5) 4/JKL

5/MNO (2.6) 5/MNO

6/PQR (2.7) 6/PQR

7/ABC (2.8) 7/ABC

8/DEF (2.9) 8/DEF

9/GHI (2.10) 9/GHI

FUNCTION KEYS (2.11) DOWN - To decrease the value of parameter set on the

READOUT in specific steps. In case of character entry moves the cursor backward by one position.

(2.12) UP To increase the value of parameter set on the READOUT in specific steps. In case of character entry moves the cursor forward by one position.

NEXT/- (2.13) NEXT - To select the next step (Eg. next value of standard and its concentration etc.) or to enter the decimal point in the Concentration value. In case of character entry this key used to change the letter case and to select the symbols mode.

ESC (2.14) ESCAPE - To quit from the present operational sequence and go back to previous operation.

NO/- (2.15) NO - To express negative response to the prompts or to enter the negative sign of the exponent of Concentration value. In case of character entry deletes single character.

YES/+ (2.16) YES - To express positive response to the prompts or to enter the positive sign of the exponent of Concentration value. In case of character entry this key used to enter into symbols menu.

ENTER (2.17) ENTER - To enable the instrument accept/enter the data parameter set or to go to the next operation.

MODE (2.18) MODE - To select different modes of operations.

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3.3.1.1 PROCEDURE TO ENTER CHARACTERS

1. Pressing a key scrolls characters present on it. Ex: Repeated pressing key 8 will scroll D, E, and F, 8 on display.

2. If no other key is pressed for a period, then cursor will advance to next position. i.e character is accepted for that position.

3. When characters required are present on two different keys then they can be pressed one after other without waiting.

4. Keep pressing on a key for more than a period of time, this will select number present on that key, and advances to next location.

Symbols Mode 1. Press NXT key till Sym is appeared in display. 2. Press YES key to enter into the symbols menu. 3. Press appropriate number to select the corresponding symbol.

3.3.2 TOP PANEL SAMPLE COMPARTMENT LID

(3) LID - To be lifted to get access to cuvettes in sample compartment. A shutter automatically protects the detector from extraneous light whenever the LID is opened.

3.3.3 REAR PANEL 90-260V AC (4) LINE FILTERED POWER RECEPTACLE - To connect

the socket end of the three-conductor power cord of the instrument with full cartridge.

ON (5) POWER OFF/ON SWITCH - to switch the instrument ON

or OFF. (6) NUMBER PLATE - To indicate the serial number of the

instrument.

3.3.4 VENTILLATION FAN-RIGHTSIDE PANEL PRINTER (7) PRINTER PORT - To connect the printer. USB (8) PC CONTROL - To connect PC to Instrument

(9) FUSE RATING STICKER - To indicates the voltage and power rating.

(Numbers in brackets of controls and readouts etc., on front and rear panel of the instrument are referred in Annexure - D)

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3.4 PRE-OPERATING INSTRUCTIONS 3.4.1 Ensure 90-260V, 50 Hz, single-phase power supply is available in the 3 contact 5A power

outlet. Cartridge fuses of 3A, 250V AC is in its place in the instrument.

The socket of the power cord of the instrument is inserted properly into the power receptacle at the rear side of the instrument-end and its 3 contact plug into line-filtered 3 contact 5A power outlet to power it.

NOTE

In case printer is proposed to be used, ensure the socket of its power cord is properly inserted in the power receptacle of the printer and the 3 contact plugs into line-filtered 3 contact 5A power outlets.

The floor of the Sample compartment is clean and devoid of any split liquid to eliminate the possibility of the vapors of split liquids vitiating the results.

3.4.2 Keep the REFERENCE SOLUTION & SAMPLE-UNDER-TEST handy.

NOTE

One REFERENCE-filled and One SAMPLE-filled cuvettes as default in the instrument and *One REFERENCE-filled and up to 6 SAMPLE filled cuvettes in photometric, concentration mode and 4 SAMPLE filled cuvettes in spectrum mode can be used in the first instance. (*Optional) The cuvettes should be thoroughly clean and devoid of fingerprints.

Annexure - E

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4.0 OPERATION FLOW 4.1 INITIALIZATION:

Switch ON the Instrument 4.1 (Screen) You find the Message on LCD as

Proceed to 4.1.1 ELICO * SL 210* DOUBLE BEAM UV-VIS SPECTROPHOTOMETER Release Vx. xxRxx PC

Followed by

4.1.1 (Screen) You find the Message on LCD as ELICO * SL 210* DOUBLE BEAM UV-VIS System is starting Please wait

Followed by Lamp Test: 4.1.2 (Screen)

< ENT > Accept and Proceed. ELICO * SL 210* DOUBLE BEAM UV-VIS Remove Cuvttes in REF, SMP Holders

4.1.3 (Screen)

You find the Message on LCD as ELICO * SL 210* DOUBLE BEAM UV-VIS D2 Lamp testing Alignment in process

Followed by 4.1.4 (Screen)

Test the D2 Lamp and display the Message on LCD as

ELICO * SL 210* DOUBLE BEAM UV-VIS

D2 Alignment OK Auto cal. In progress


Display the Message after completion of the Lamps Test.


W Lamp Alignment OK * Lamps Test Over *

Baseline Scan: 4.1.6 (Screen)

< YES > Go to 4.1.7 < NO > Go to 4.1.9


Start Base line Scan?

4.1.7 (Screen)

Display the Message till an instrument generates its baseline data.

ELICO * SL 210* DOUBLE BEAM UV-VIS BASE LINE SCAN Under

Progress Followed by 4.1.8 (Screen)

You find the Message on LCD as Press Any Key to Proceed.

ELICO * SL 210* DOUBLE BEAM UV-VIS Base Line Scan Over

Press any key

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Main Menu: 4.1.9 (Menu)

You find the Message on LCD as.

-------------* MENU *-------------- 1. SPECTRUM 2. MULTI l 3.TIMESCAN 4. SYSTEM 5. QUANTITATIVE 6. PC

Note: To use any of modes available you need to perform FULL RANGE baseline scan at least once.

4.2 SPECTRUM: Press key, if scanning is desired in

Modes Menu

4.2.1 (Screen)

Default SWL = 190.0 nm < # > Wavelength Entry < NO > Clear the field entry pointed < ESC > Go to Main Menu. < ENT > Accept the Values and Proceed.

--------------* Spectrum *---------- Enter scan limits SWL: 190.0 nm EWL: nm

4.2.2 (Screen)

AZ Select and proceed to 4.2. 3 AZ Not Select and proceed to 4.2.7

-------------* Spectrum *---------- You Want Auto Zero Option?

4.2.3 (Screen)

< ENT > Accepts the Reference and Proceed. < ESC > Go to 4.2.2

--------------* Spectrum *---------- Place REF & SMP Cuvettes with BLANK


Display the message For a while

--------------* Spectrum *---------- Preparing to read

Please wait Followed by 4.2.5 (Screen)

Display the Message While Scanning REF CUVETTES Percentage progress also displayed.

--------------* Spectrum *---------- l RANGE: (XXX.X - XXX.X) Scanning REF Progress xx %


< ENT > Accepts the SAMPLE and proceed to 4.2.8

--------------* Spectrum *---------- Place the Sample

4.2.7

Accepts the REF & SAMPLE and proceed

--------------* Spectrum *---------- Place REF & SMP

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4.2.8 (Screen)

Display the message For a while

--------------* Spectrum *---------- Preparing to read

Please wait Followed by 4.2.8.1 (Screen)

Display the Message While Scanning SAMPLE CUVETTES. Percentage progress also displayed.

--------------* Spectrum *---------- l RANGE: XXX.X - XXX.X Scanning SMP Progress xx %

Wait till you find the following on LCD display

4.2.9 (Menu)

< # > Take Choice. --------------* Spectrum *---------- 1.VIEW 4. MENU 2.PRINT 5. AUTOZERO 3. REPEAT

4.2.9.1 Spectrum VIEW: Press key, if it is desired to view the

Scan data, If AZ not Selected you finds the message on LCD as.

4.2.9.1.1 (Screen)

? increment, decrement < # > ? Entry < ESC > Go to 4.2.9

--------------* Spectrum *---------- l: XXX.X nm AZ Abs = Y.YYYY

%T = YY.Y < #, ? , ? > If AZ Selected you find the message on

LCD as.

4.2.9.1.2 (Screen)

? increment, decrement < # > ? Entry < ESC > Go to 4.2.9 Display AZ status ON and

OFF Results.

--------------* Spectrum *---------- l: XXX.X nm AZ Abs = Y.YYYY %T = YY.Y < #, ? , ? , NXT >

4.2.9.2 Press key, if it is desired to quit 4.2.9.2.1 (Screen)

< YES > Go to Main Menu. < NO > Go to 4.2.9

--------------* Spectrum *---------- DATA will be LOST! OK

4.2.9.3 Spectrum PRINT: 4.2.9.3.1 (Screen)

Press key, if it is desired to print data --------------* Spectrum *---------- 1.VIEW 4. MENU 2.PRINT 5. AUTOZERO 3.REPEAT

Followed by If AZ Selected Proceed to 4.2.9.3.2

If AZ Not Selected Proceed to 4.2.9.3.3

4.2.9.3.2 (Screen)

Change AZ Status ON and OFF.

Accept AZ Status and Proceed

------------* Spectrum *---------- Select AZ Status To Process DATA AZ < ON > < NXT, ENT >

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4.2.9.3.3 (Screen)

Press to print with HP printer Press to print with other than HP.

------------* Spectrum *---------- Select Printer 1. HP 2. Others Select option

If Printer is Ready Proceed to 4.2.9.3.5 If Printer is Not Ready Proceed to

4.2.9.3.4

4.2.9.3.4 (Screen)

Press to check printer again Press to back to 4.2.9

------------* Spectrum *---------- PRINTER NOT READY

1. Retry 2. Cancel < # >

4.2.9.3.5 (Screen)

< YES > Go to 4.2.9.3.6 < NO > Quit and Proceed.

------------* Spectrum *----------

WISH TO CHANGE NAMES

4.2.9.3.6 (Screen)

< ENT > Proceed to Enter the Analyst Name.

------------* Spectrum *---------- ENTER ANALYST NAME ---

4.2.9.3.7 (Screen)

< ENT > Enter Organization name and Proceed.

------------* Spectrum *---------- ENTER ORGANISATION: ---

4.2.9.3.8 (Screen)

< ENT > Enter Reference name and Proceed.

------------* Spectrum *---------- ENTER REFERENCE: ---

4.2.9.3.9 (Screen)

< ENT > Enter Sample name and Proceed.

------------* Spectrum *---------- ENTER SAMPLE: --

4.2.9.3.10 (Screen)

Displays the Date ,Time & corresponding day and goes to 4.2.9.3.11

------------* Spectrum *---------- Xx-xx-xxxx DAY Y-YY-YY

4.2.9.3.11 (Screen)

Enter Wavelength to Print and Press To proceed.

------------* Spectrum *---------- PRINT RANGE SWL=XXX.Xnm EWL=XXX.Xnm

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4.2.9.3.12 (Screen)

Press to select Abs and go to 4.2.9.3.13 Press to select %T and go to 4.2.9.3.19

------------* Spectrum *---------- Select Data To Print 1.Abs 2. %T Select Option?

ABSORBANCE: 4.2.9.3.13 (Screen)

Press Key, if DATA is to be printed and go to 4.2.9.3.14 Press Key, if GRAPH is to be printed and go to 4.2.9.3.16

------------* Spectrum *---------- Select Print Option 1.TABLE 2.GRAPH < # >

4.2.9.3.14 (Screen)

Enter Print Interval ------------* Spectrum *---------- Enter Print Interval Range (0.1-1.0) nm : < #, ENT >

Followed by 4.2.9.3.15 (Screen)

Display the message as ------------* Spectrum *---------- Printing Under Progress Please wait

Followed by With * blinking. 4.2.9.3.16 (Screen)

Enter Lower Limit and Higher Limit & press

------------* Spectrum *---------- Enter Abs Axis Limits LO: HI:


Displays the message for a while ------------* Spectrum *---------- Calculation Under Progress. Please wait

4.2.9.3.18 (Screen)

After completing calculation it prints the Graph and goes to 4.2.9

--------------* Spectrum * ---------- Printing Under Progress Please wait

% Transmission: Press < 2 > key, to select %T in 4.2.9.3.12

4.2.9.3.19 (Screen)

Press if data is to be printed & go to 4.2.9.3.14 Press if data is to be printed & go to 4.2.9.3.20

------------* Spectrum *---------- Select print option 1.Table 2.Graph < # >

4.2.9.3.20 (Screen)

Enter Lower Limit and Higher Limit & press

--------------* Spectrum *---------- Enter %T Axis Limits LO: HI:

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4.2.9.3.21 (Screen)

Displays the message for while --------------* Spectrum *---------- Calculation Under Progress. PLEASE WAIT

With * blinking. 4.2.9.3.22 (Screen)

After completing calculation it prints the Graph

--------------* Spectrum *---------- PRINTING under Progress. PLEASE AIT

Followed after printing and goes to 4.2.9 4.2.9.3.23 (Screen)

Press to Quit from Spectrum mode Press to Quit Pressto remain in the same mode

--------------* Spectrum * Data will be Lost ! OK

4.3 PHOTOM ETRIC: 4.3.1 (Menu)

Press key, if the photometric mode is desired

-------------* MENU *-------------- 1. SPECTRUM 2. MULTI l 3.TIMESCAN 4. SYSTEM 5. QUANTITATIVE 6. PC

4.3.2 (Screen)

< # > Wavelength Entry < ENT > Accept the Values and Proceed Stops l Entry and Proceed. < ESC > Go to Main Modes.

------------*Multi l *------------- Enter Wavelengths. l01: xxx nm (MAX-16) < #, ENT, NO >

With * blinking.

4.3.3 (Screen)

< YES > Go to 4.3.2 < NO > Proceed to 4.3.4.

------------*Multi l *------------- XX * ls entered. Wish to change/check ?

With * blinking. 4.3.4 (Screen)

< # > Entry of Samples number < ENT > Accept and Proceed.

------------*Multi l *------------- Enter No. of Samples to be scanned: X (MAX 6) < #, ENT >

4.3.5 (Screen)

AZ Selected and Proceed to 4.3.6 AZ Not Selected and Proceed to

4.3.11

------------*Multi l *------------- You Want Auto Zero Option?

4.3.6 (Screen)

Accept REF in Both Cuvettes and Proceed.

< ESC > Go to 4.3.5

------------*Multi l *------------- Place REF & SMP Cuvettes with BLANK

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4.3.7 (Screen)

Display Message on LCD for a While. ------------*Multi l *------------- Preparing to read Please wait..


Display Message on LCD While Reading REF Cuvettes.

------------*Multi l *------------- Reading REF XX of XX At XXX.X nm Please Wait . . .

4.3.9 (Screen)

If Number of References more than one you find the Message on LCD. Or Else Proceed to

4.3.10 < ENT > Go to 4.3.7.and return to 4.3.10

------------*Multi l *------------- Place next BLANK < ENT >

4.3.10 (Screen)

< ENT > Accept the Samples and Proceed to 4.3.12

------------*Multi l *------------- Place the Samples

4.3.11 Accept the Samples and proceed.

Go to 4.3.5 ------------*Multi l *------------- Place Ref & Smp

4.3.12 (Screen)

Display Message on LCD for a While.

------------*Multi l *------------- Preparing to read

Please wait.


Display Message on LCD While Reading SAMPLE.

------------*Multi l *------------- Reading SAMPLE XX of XX At XXX.X nm Please Wait . . .

4.3.14 (Screen)

If No of Samples more than one you find the Message on LCD. Or Else Proceed to 4.3.15. < ENT > Go to 4.3.12

------------*Multi l *------------- Place next Sample < ENT >


< # > Take choice. ------------*Multi l *------------- 1. VIEW 4. Menu 2. PRINT

3. Auto Zero < # > 4.3.15.1 Photometric VIEW: 4.3.15.1.1 (Screen)

Press key, if it is desired to view the data

------------*Multi l *------------- 1.VIEW 4. Menu 2. PRINT

3. Auto zero

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Followed by If AZ Selected proceed to 4.3.15.1.2

If AZ Not Selected Proceed to 4.3.15.1.3

4.3.15.1.2 (Screen)

< NXT > AZ Status on or off < ENT > Accept and Proceed to 4.3.15.1.3 < ESC > Go to 4.3.15

------------*Multi l *------------- Select AZ Status To Process DATA AZ < ON > < NXT, ENT >


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4 9

STANDARD METHOD: Press key, if the concentration is desired

to be measured by STANDARD METHOD.

4.4.1.1 (Screen)

< # > Wavelength Entry. < ENT > Accept and Proceed. < ESC > Go to 4.4.0

--------*Quantitative*--------- Select l: XXX.X nm

< #, ENT >

With * blinking. 4.4.1.2 (Screen)

< # > Enter Standard number. < ENT > accept and Proceed < ESC > Go to 4.4.0

--------*Quantitative*--------- Enter Number of Standards: * (Max -- 10) < #/ENT >

With * blinking. NOTE: Minimums of 2 standards are required for the test. SELECTION OF UNITS:

4.4.1.3 (Screen)

< # > Select units --------*Quantitative*--------- Select Conc. Units 1) Moles 2) ppm < # >

Moles UNIT SELECTED Press key, if the units of Concentration

desired M (Moles/litre).

4.4.1.4 (Screen)

< # > Enter Concentration < ENT > Accept and Proceed < ESC > Go to 4.4.1.3

--------*Quantitative*--------- ENTER STD 1 CONC.

*E-..M < #, ENT >

Proceed to step 4.4.1.7 With * blinking. Press key, if the units of concentration

desired are ppm.

4.4.1.5 (Screen)

< # > Enter Concentration < ENT > Accept and Proceed < ESC > Go to 4.4.1.3


-------ppm < #, ENT>

If the calibration data of the standards and the

units of their Concentration are already entered, this message will displayed

4.4.1.6 (Screen)

< YES > Proceed. < NO > Go to 4.4.1.5

--------*Quantitative*--------- DO YOU WANT TO USE OLD CAL DATA!

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4 10

4.4.1.7 (Screen)

< # > Take Choice. < ENT > Accept and Proceed

--------*Quantitative*--------- Select mode of Abs Input.

1. MEASURE 2. KEY-IN

CALIBRATION: KEYING-IN THE VALUES OF CONCENTRATION OF STANDARDS IN M. 4.4.1.7.1 (Screen)

< # > Enter Concentration in MOLE. < +/- > Enter Sign. < ENT > Accept and Proceed.


*E-.M < #, ENT >

With * blinking.

KEYING-IN THE VALUES OF CONCENTRATION OF STANDARDS IN PPM

4.4.1.7.2 (Screen)

< # > Enter Concentration. In ppm < ENT > Accept and Proceed.


-*------ppm < #, ENT>

With * blinking. 4.4.1.8 MEASURE: Press key in 4.4.1.7 if the Absorbance

input is to be measured from the standard solution.

4.4.1.8.1 (Screen)

Enter the Number of Samples Analyzed. < ENT > Accept and Proceed.

--------*Quantitative*--------- Enter no. Of samples Max- 16 * < #, ENT >

4.4.1.8.2 (Screen)

< YES > AZ Selected and Proceed to 4.4.1.8.3 < NO > AZ Not Selected and Proceed to 4.4.1.8.8

--------*Quantitative*--------- You Want Auto Zero Option?

4.4.1.8.3 (Screen)

< ENT > Accepts the Reference and Proceed.

< ESC > Go to 4.4.1.8.2

--------*Quantitative*--------- Place REF & SMP Curettes with BLANK

4.4.1.8.4 (Screen)

Display the Message for a while

--------*Quantitative*--------- Preparing to read Please wait . . .


Display the Message While Reading REF CUVETTES.

--------*Quantitative*--------- Reading BLANK X At XXX.X nm PLEASE WAIT . . .

BLANK no. and its WL is displayed. 4.4.1.8.6 (Screen)

If Reference More than one, You find the Message on LCD and Go to 4.4.1.8.5. If Reading References Over Go to 4.4.1.8.7

--------*Quantitative*--------- Place next BLANK

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4 11

4.4.1.8.7 (Screen)

< ENT > Accept the standards and Proceed. --------*Quantitative*--------- Place the Standards < ENT >

4.4.1.8.8 (Screen)


--------*Quantitative*--------- Preparing to read

Please Wait.


Display the Message While Reading.

--------*Quantitative*--------- Reading Standard X At XXX.X nm PLEASE WAIT . . .

Standards no. and its WL is displayed.

4.4.1.8.10 (Screen)

If Standards More than one, You find the Message on LCD. And Go to 4.4.1.8.9 after Press key . If reading Standards Over Go to 4.4.1.8.11

--------*Quantitative*--------- Place next standard < ENT >

4.4.1.8.11 (Menu)

Press key, if it is desired to view the data..

--------*Quantitative*--------- 1.VIEW 4. Save 2. Samples 5. Menu 3. Auto Zero < # >

Proceed to step 4.7 4.4.1.8.12 KEY-IN Press key in 4.4.1.7 to enter into key in

mode.

4.4.1.8.13 (Screen)

Enter Abs values for Standard 1and Press ENT key.

--------*Quantitative*--------- Enter Abs of STD 01 X.XXX (-3 to +3) < #, ENT >

4.4.1.8.14 (Screen)

If more than one standard entered it again asks to enter Abs value for second standard and press ENT key.

--------*Quantitative*--------- Enter Abs of STD 02 X.XXX (-3 to +3) < #, ENT >

4.4.1.8.15 (Screen)

Enter number of samples and press ENT --------*Quantitative*--------- Enter no. of samples (Max-16): < #, ENT>

4.4.1.8.16 (Screen)

Display shows the message as --------*Quantitative*--------- Preparing to read Please wait

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4 12

4.4.1.8.17 (Screen)

Display shows the message as

--------*Quantitative*--------- You want Auto zero Option for samples?

Proceed to 4.4.1.8.2 to 4.4.1.8.10 (Screen),

IN place of standards it displays samples.

4.4.1.8.18 (Screen)

After completing 4.4.1.8.10 (Screen) it displays the message as

--------*Quantitative*--------- 1.View 4.Menu 2.Print 5.Save 3.Autozero

Proceed to 4.7 4.4.2. -FACTOR METHOD: 4.4.2.1 Press key, if concentration is desired to

be measured in -factor method.


< # > Wavelength Entry < ENT > Accept and Proceed. < ESC > Go to 4.4.1

--------*Quantitative*--------- SELECT l * nm < # , ENT >

4.4.2.3 (Screen)

< # > Enter Concentration. < +/- > Enter Sign. < ENT > Accept and Proceed

--------*Quantitative*--------- ENTER -FACTOR

E - M < # , ENT >

4.4.2.4 (Screen)

< # > Enter the No of Samples. < ENT > Accept and Proceed. < ESC > Go to 4.4.2.2

--------*Quantitative*--------- ENTER NO. OF SAMPLES Max. -16 * < # , ENT >


< YES > AZ selected and Go to 4.4.2.6

< NO > AZ Not Selected and Go to 4.4.2.11

--------*Quantitative*--------- You Want Auto Zero Option?

4.4.2.6 (Screen)

< ENT > Accept References and proceed.

< ESC > Go to 4.4.2.5

--------*Quantitative*--------- Place REF & SMP Cuvettes with BLANK

4.4.2.7 (Screen)


--------*Quantitative*--------- Preparing to read

Please wait

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4 13

4.4.2.8 (Screen)

Display the Message While Reading BLANK no. and its WL is displayed.

--------*Quantitative*--------- READING BLANK -x

at xxx.x nm Please wait . . .

Followed by

4.4.2.9 (Screen)

If Reference More than one, You find the Message on LCD, and Proceed to 4.4.2.8 If Reading References Over Go to 4.4.2.10 For AZ YES Display the message.

--------*Quantitative*--------- Place next BLANK

4.4.2.10 (Screen)

For AZ NO Display the message < ENT > Accept Samples and proceed

--------*Quantitative*--------- Place the Sample

4.4.2.11 (Screen)


--------*Quantitative*--------- Preparing to read Please wait

4.4.2.12 (Screen)

Display the Message While Reading

--------*Quantitative*--------- READING Sample -x

at xxx.x nm Please wait . . .

Sample no. and its WL is displayed. 4.4.2.13 (Screen)

If Sample More than one, You find the Message on LCD., and Proceed to 4.4.2.12

If Reading Samples Over Go to 4.4.2.14

--------*Quantitative*--------- PLACE NEXT SAMPLES < ENT >

Followed by 4.4.2.14 (Menu)

--------*Quantitative*--------- 1. VIEW 4. Menu. 2. PRINT

3. Auto Ze ro < # > 4.4.2.15 VIEW - DATA OF SAMPLES:

Press key, if it is desired to view the samples data. If AZ Selected You find the Message on LCD. If AZ Not Selected Go to 4.4.2.17

4.4.2.16 (Screen)

AZ Status on or off < ENT > Accept and Proceed. < ESC > Go to 4.4.2.14

--------*Quantitative*--------- Select AZ Status To Process DATA AZ < ON > < NXT, ENT >

4.4.2.17 (Screen)

SL 210 USERS MANUAL

4 14

4.4.2.18 PRINT - DATA OF SAMPLES: Press key, if it is desired to print the

data of Sample in 4.4.2.14

4.4.2.19 (Screen)

You Find the Message on LCD as

--------*Quantitative*--------- 1. VIEW 4. Menu. 2. PRINT 3. Auto zero

Results on Printer If AZ Selected Go to 4.4.2.20

If AZ Not Selected Go to 4.4.2.21

4.4.2.20 (Screen)

< NXT > AZ Status on or off < ENT > Accept and Proceed. < ESC > Go to 4.4.2.14 Followed by 4.3.15.3.2


4.4.2.21 (Screen)

Display Message while Printing. --------*Quantitative*--------- PRINTINGUNDERPROGRES

PLEASE WAIT After printing Over Flow goes to

4.4.2.14 NOTE

Ensure Printer is connected, powered and is in ON-LINE mode. Data is presented in tabular form only.

4.4.3 AUTOZERO: Press key in 4.4.2.14, to Enter into Auto

Zero mode And goes to 4.4.2.6

4.4.4 MODES: Press key, to quit from the Data

presentation mode.

4.4.4.1 (Screen)

You Find the Message on LCD < YES > if it is desired to quit and goes

to Main Menu. < NO > Go to 4.4.2.14

--------*Quantitative*--------- DATA Will be LOST! OK

4.4.5 SAMPLES: Press key, if it is desired to measure the

samples.

4.4.5.1 (Screen)

You Find the Message on LCD. < ESC > Go to 4.4.1

--------*Quantitative*--------- Load STD File for Sample measurement

Proceed to 4.4.1.8.15

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4 15

4.4.6 FILE: Press key, if it is desired select the file

options in 4.4.1

4.4.6.1 (Screen)

You Find the Message on LCD. < ESC > Go to 4.4.1

---------- *FILES*---------- 1. View 2. Load

3. Print. 4. Delete 4.4.7 FILE VIEW:

Press key, if it is desired to view the data of the saved files.

4.4.7.1 (Screen)

SL 210 USERS MANUAL

4 16

4.4.9.4 (Screen)

Display the Message While Loading.

---------- *FILES*---------- FILE LOADING WAIT A MOMENT

Proceed to 4.4.5 4.4.10 FILE DELETE: Press key, if it is desired to delete the files. 4.4.10.1 (Screen)

SL 210 USERS MANUAL

4 17

4.5.2 BASELINE: Press it is desired to select wavelength set

depending on the spectral range of use

4.5.2.1 (Screen)

< YES > Go to 4.5.2.2 < NO > Go to 4.5.2.3

------------*BASELINE*----------- Do you Want to Select Scan range < YES/NO>

4.5.2.2 (Screen)

< # > Enter Wavelength. < ENT > Accept and Proceed. < ESC > Go to 4.5.2.1

------------*BASELINE*----------- Enter Scan Limits SWL: 190.0 nm EWL: nm < #, ENT >

4.5.2.3 Displays the message and press ENT to

proceed.

------------*BASELINE*----------- Remove cuvettes in REF, SMP Holders

4.5.2.4 Press to start Baseline Scan

Press Go to 4.5 After completing Baseline Scan it displays Baseline Scan over press any key and goes to 4.5 after Pres

------------*BASELINE*----------- Start Baseline Scan?

4.5.3 DIAGNOSTICS:

Press it is desired to enter Diagnostics

4.5.3.1 (Screen)

Type Password. < ENT > Accept and Proceed. < ESC > Go to 4.5

----------* DIAGNOSTICS* ------ Enter PASSWORD: ABC < ENT/ESC >

4.6 TIME SCAN: Press key, if is desired to select Time Scan Mode in Main Modes 4.6.1 (Screen)

< # > Wavelength Entry < ENT > Accept and Proceed < ESC > Go to Main Menu.

-----------TIME SCAN---------- ENTER WAVELENGTH: XXX .X nm.

4.6.2 (Screen)

< # > Enter Scan Interval in Seconds. < ENT > Accept and Proceed < ESC > Go to 4.6.1

-----------TIME SCAN--------- ENTER SCAN INTERVAL:

Sec. (1.0 - 60)

4.6.3 (Screen)

< # > Enter Scan Period in Minutes. < ENT > Accept and Proceed < ESC > Go to 4.6.2

-----------TIME SCAN--------- ENTER SCAN PERIOD: mnts. (Max 16)

SL 210 USERS MANUAL

4 18

4.6.4 (Screen)

AZ selected and Proceed to 4.6.5

-----------TIME SCAN--------- You Want Auto Zero Option?

AZ not Selected and proceed to 4.6.8 -----------TIME SCAN---------

Place Reference in REF Holder

4.6.5 (Screen)

Display Message For a While. -----------TIME SCAN--------- Preparing to read Please wait

4.6.6 (Screen)

< ENT > Accept Reference in REF and SMP Cuvettes and Proceed.

< ESC > Go to 4.6.4

-----------TIME SCAN--------- Place REF & SMP Cuvettes with BLANK

4.6.7 (Screen)

Display Message While Reading REF CUVETTES.

-----------TIME SCAN--------- Scanning REF Please Wait . . .

4.6.8 (Screen)

< ENT > Accept Sample in SMP Cuvette and Proceed

-----------TIME SCAN--------- Place Sample in SMP holder

4.6.9 (Screen)

You Find the Message on LCD While Scanning Sample

-----------TIME SCAN--------- l XXX.X nm TP P .P Min Abs Y.YY.YY TI Q.Q Sec Time RR: R sec


If we press Time scan is being interrupted.

-----------TIME SCAN--------- SCAN INTERRUPTED 1.VIEW 3.AUTOZERO 2.PRINT 4.MENU

Time Scan VIEW: Press key in 4.6.10.9 4.6.10.1 (Screen)

If AZ Selected Find Message on LCD as Switch to ON or OFF < ENT > Accept and Proceed. < ESC > Go to 4.6.10.9

-----------TIME SCAN--------- Scanned Data AZ

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4 19

4.6.10.2 (Screen)

If AZ Not Selected Find Message on LCD as.

-----------TIME SCAN--------- 1. Scanned Data 2. Peak Abs 3. Parameters

Press key in 4.6.10.2 4.6.10.3 (Screen)

You Find the Message on LCD as < ESC > Go to 4.6.10.2

-----------TIME SCAN--------- l XXX.X nm TP P .P Min Abs Y.YY.YY TI Q.Q Sec Time RR: R


< ENT > Proceed to Next Screen. < Esc > Go to 4.6.10.2

-----------TIME SCAN--------- Maximum Abs: Y.YYYY Max. Abs Time: Q. Q Sec

4.6.10.5 (Screen)

< ENT > Proceed to Previous Screen. < Esc > Go to 4.6.10.2

-----------TIME SCAN--------- Minimum Abs: YYY. Y Min. Abs Time: Q. Q Sec


You Find the Message on LCD as < ESC > Go to 4.6.10.2

-----------TIME SCAN--------- Time interval: Q. Q Sec Time Period: P. P Min

Press key in 4.6.10.9 for Time Scan PRINT

4.6.10.7 (Screen)

Press to select Auto zero and press

-----------TIME SCAN--------- Select AZ status To process DATA AZ

Followed by 4.6.10.8 (Screen)

Display Message on LCD While Printing after proceed to 4.3.15.3.2

-----------TIME SCAN--------- Printing Under Progress Please Wait . . .


You Find the Message on LCD as DATA will be Lost! OK < YES > Go to Main Menu. < NO > Go to 4.6.10.9

-----------TIME SCAN--------- SCANNING OVER. 1.View 3. Auto zero 2.Print 4.Menu

4.7 STANDARDS PRESENTATION: 4.7.1 VIEW: Press key, if it is desired to view the data of

the Standards. If AZ Selected Go to 4.7.1.1 If AZ Not Selected Go to 4.7.1.2

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4 20

4.7.1.1 < NXT > AZ Status on or off < ENT > Accept and Proceed. < ESC > Go to Menu.


If the Units of Concentration selected as ppm.

4.7.1.2

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4 21

Proceed to Main Menu. 4.7.2.5 If file name already exists

< ESC > Go to Menu. --------*Quantitative*--------- FILE ALREADY EXIST SAVE NOT POSSIBLE

4.7.2.6 If space is not sufficient to save the file

< ESC > Go to Menu. --------*Quantitative*--------- Memory full Save Not possible

4.8 SAMPLES PRESENTATION Press key, if it is desired to measure the

samples.

< YES > AZ Selected < NO > AZ Not Selected Proceeds to 4.7.1.2 shows samples in place of standard displayed in the screen.

--------*Quantitative*--------- You Want Auto Zero option for samples? < YES/NO >

4.9 MODES: Press key, to quit from the Data Presentation mode. Proceed to Main Menu. 4.9.1 PC Mode: PC.0

If Communication is established then the flow goes to PC.1 If Communication with

PC failed, flow goes to PC.2

------------*PC MODE*---------- Checking for PC Please Wait

PC.1

Display Message While Transferring Data

------------*PC MODE*---------- Transferring Base Line Factors to PC

PC.2

< YES > Flow goes to PC.0 < NO > Flow goes to Main Menu.

------------*PC MODE*--------- PC Not Ready Retry?

IM 1 (Screen)

This message occurs when the RAM is failed

ELICO * SL 210* DOUBLE BEAM UV-VIS RAM Failure System Halted

IM 2 (Screen)

This message occurs when the home for filter Drive is not detected within the

predefined no of Steps.

ELICO * SL 210* DOUBLE BEAM UV-VIS Filter Home Fail System Halted

SL 210 USERS MANUAL

4 22

IM 3 (Screen)

This message occurs when the home for grating Drive is not detected within the


ELICO * SL 210* DOUBLE BEAM UV-VIS Grating Home Fail System Halted.

IM 4 (Screen)

This message occurs when the home for Lamp Drive is not detected within the


ELICO * SL 210* DOUBLE BEAM UV-VIS Lamp Home Fail System Halted.

Apart from these 6 options in Main Menu we have another mode called Instant Mode

SL 210 USERS MANUAL

5 1

5. CHECKING THE PERFORMANCE 5.1 PRE-PERFORMANCE CHECK INSTRUCTIONS Perform all the pre-operating instructions and keep REFERENCE empty & and

BENZENE VAPOUR (C6H6)-filled Cuvette as sample in the SAMPLE position of the Cuvette holder.

NOTE

The Cuvettes should be thoroughly clean and devoid of fingerprints.

CAUTION

Insert cuvettes vertically in the positions of Cuvette holder 1 & 2 without imparting any lateral motion to the cuvette holder, to avoid misalignment of position of cuvettes. If any lateral motion is imparted start the check test over again.

5.2 SPECTRAL CALIBRATION 5.2.1 Select SPECTRUM MODE by performing operations of step 4.2.0. 5.2.2 Key-in Starting WL of 240 nm and Ending WL of 270 nm and scan the spectral

range selected performing operations of steps 4.2.1 to 4.2.9.1.2 If the Wavelength at the Peak Absorbance as read in the Graph Table is 253.9

0.5 nm, the spectral calibration of the instrument in UV spectral region is confirmed to be proper.

5.3 PHOTOMETRIC PERFORMANCE Select PHOTOMETRIC MODE by performing operations of step 4.3.0.

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5 2

5.3.1 ABSORBANCE 5.3.1.1 Prepare 60 ppm K2Cr2O7 in 0.1N H2SO4. Select 257 nm as Wavelength WL-1 and

place 0.1N H2SO4 as blank in REFERENCE and 60 ppm potassium dichromate as test sample in SAMPLE and view or print ABSORBANCE of test sample at 257 nm by performing operations of steps 4.3.1 to 4.3.15.3.2

If the value of ABSORBANCE of Sample is 0.864 0.01, it is confimed that the instrument is measuring ABSORBANCE correctly.

5.3.2 PERCENTAGE TRANSMISSION 5.3.2.1 As the value of PERCENTAGE TRANMISSION is derived form

ABSORBANCE itself, if the instrument is measuring ABSORBANCE correctly it can be assumed that it measures PERCENTAGE TRANSMISSION also correctly.

5.3.3 CONCENTRATION 5.3.3.1 Prepare and keep handy potassium permanganate (KMnO 4) solution of

Concentrations 10 ppm as STD-1, 20 ppm as STD-2, 30 ppm as STD-3, 15 ppm as SMP-1. Place H2O as REFERENCE.

5.3.3.2 Select CONCENTRATION MODE, STANDARD METHOD of measurement, ppm

as CONCENTRATION UNITS and 545 nm as WAVELENGTH, by performing operations of steps 4.4.1.7.1 to 4.4.1.8.11 measure and view concentration in ppm of SMP-1.

5.3.3.3 If the value of Concentration displayed for the SMP-1 is 15 1.0 ppm, it is

confirmed that the instrument is measuring Concentration correctly.

SL 210 USERS MANUAL

6 1

6. TROUBLE SHOOTING

INDICATION POSSIBLE FAULT REMEDY

6.1

No display on LCD Display.

1. 2.

Plug of power cord of the instrument not inserted in the power outlet. Fuse at the power.

1. 2.

Insert Properly. Check the fuse, if its blow off, replace Fuse.

6.2 Prompt

Verify UV region

1. Press YES for retry. 1. Switch OFF and wait for more than 2 minutes & switch ON again.

2. Press NO for moving further.

2. If W Lamp is OK then the problem may be less energy or D2 Lamp Power supply problem, correct it or D2 Lamp life is over. Lamp to be replaced. If alignment problem, re align.

6.3 Prompt

Check visible region Use (190.0nm to 379.9nm)

1. Visible region failed 1. W Lamp power supply may be problem correct it or Lamp filament blow off. Lamp to be replaced If alignment problem, re align

NOTE

For any other fault return the instrument to your nearest ELICO Sales and Support Center.

SL 210 ANNEXURE-A

A 1

COLORIMETRY AND SPECTROPHOTOMETRY 1. INTRODUCTION 1.1 Colorimetry is concerned with the determination of the concentration of a substance

by measurement of relative absorption of light with respect to a known concentration of the substance. In Colorimetry, white light is generally used as a light source, and detections are usually made with photoelectric cell. In COLORIMETERS light contained within a comparatively narrow range of wavelengths furnished by passing white light through filters of colored glass, gelatin etc. is employed, transmitting only limited spectral region. COLORIMETERS are also called FILTER PHOTOMETERS.

1.2 In SPECTROPHOTOMETRY source of radiation used extends into the ultraviolet region of the spectrum, from which definite wavelengths of radiation are chosen possessing a bandwidth of less than 1 nm. In optical spectrometer measurements can be made of the quantity of transmitted radiation. In SPECTROPHOTOMETER, signal corresponding to the difference between the transmitted radiation of a reference material and that of a sample at selected wavelengths are measured.

1.3 Colorimetric and Spectrophotometric methods provide a simple means for

determining minute quantities of substances. 2 THEORY OF COLORIMETRY & SPECTROPHOTOMETRY 2.1 When light (monochromatic or heterogeneous) falls on a homogeneous medium, a

portion of the incident light is reflected, a portion is absorbed within the medium, and the remainder is transmitted. If the intensity of the incident light is expressed by Io, that of the absorbed light by Ia, that of the transmitted light by It and that of the reflected light by Ir then:

Io = Ia + It + Ir (1)

Ir is usually eliminated by the use of comparison cells, as it is a mere 4% only, rendering.

Io = Ia + It (2)

2.2 As per Lambert's Law when monochromatic light passes through a transparent medium, intensity of the emitted light decreases exponentially as the thickness of the absorbing medium increases arithmetically, expressed by

It = Io.e-kl (3)

Where Io is the intensity of the incident light falling upon an absorbing medium of thickness l unit. It is the intensity of the transmitted light and k is a constant for the wavelength and the absorbing medium used.

It = Io 10 -0.4343kl = Io.10-kl (4)

SL 210 ANNEXURE-A

A 2

Where K = k / 2.3026 and is usually termed the ABSORPTION COEFFICIENT generally defined as the reciprocal of the thickness (l cm) required to reduce the light to 1/10 of its intensity. The ratio It /Io is the fraction of the incident light transmitted by a thickness l unit of the medium and is termed the TRANSMITTANCE T. Its reciprocal Io/It is the OPACITY and the ABSORBANCE A of the medium (formerly called the OPTICAL DENSITY OD) is given by:

A = log (Io / It) (5)

2.3 As per Beer's law the intensity of a beam of monochromatic light decreases exponentially as the concentration of the absorbing substance increases arithmetically. This may be written in the form:

It = Io.e-k'c = Io.10-0.4343k'c = Io.10 -K'c (6)

Where c is the concentration, and k' and K' are constants. Combining equation (5) and (6), we have:

It = Io = 10-acl or log (Io / It) = acl (7) 2.4 This is the fundamental equation of COLORIMETRY and

SPECTROPHOTOMETRY, and is the principle of the famous BEER-LAMBERT LAW. The value of A will clearly depend upon the method of expression of the concentration. If c is expressed in mole L-1 and l in centimeters then a is given symbol e and is called the MOLAR ABSORPTION COEFFICIENT or molar absorptivity. The specific absorption coefficient es (sometimes termed absorbency index) may be defined as the absorption per unit thickness (path length) and unit concentration.

Thus there is a relationship between the ABSORBANCE A, the TRANSMITTANCE T, and the molar absorption coefficient, since:

A = cl = log (Io / It) = log 1/T = - log T (8)

2.5 SPECTROPHOTOMETERS are often calibrated to read directly in ABSORBANCE

and also in PERCENTAGE TRANSMITTANCE. For colorimetric measurements Io is usually understood as the intensity of the light transmitted by the pure solvent, or the intensity of the light entering the solution. It is the intensity of the light emerging from the solution or transmitted by the solution. It will be noted that:

The MOLAR ABSORPTION COEFFICIENT is the specific absorption coefficient for a concentration of I mole L-1 and a path length of 1 cm.

= A / cl (9)

SL 210 ANNEXURE-A

A 3

2.6 If two solutions of a colored substance with concentrations of c1 and c2 are placed in an instrument in which the thickness of the layers can be altered and measured easily which also allows a comparison of the transmitted light and both layers have the same colour intensity:

It1 = Io.10-l1C1 = It2 = Io.10-l2c2 (10)

Where l1 and l2 are the lengths of the columns of solutions with concentrations c1 and c2 respectively when the system is optically balanced. Under these conditions, as per Beer's Law:

l1 c1 = l2 c2 (11) 2.7 If the concentration c1 is known concentration Cx of an unknown solution can be

calculated from:

Cx = [log (Io / It)] / l (12)

When MOLAR ABSORBTION COEFFICIENT, which depends on wavelengths of incident light, temperature and solvent, employed is known and matched cells are used (i.e.c1 is constant).

c a log (Io/ It) or c a log (1/T) or C a A (13)

Hence, by plotting A (or log (1/T)), as ordinate, against concentration as abscissa, a straight line will be obtained and this will pass through the point c=0,A=0 (T=100%). This calibration line may then be used to determine unknown concentrations of solutions of the same. Material after measurement of absorbances.

NOTE

THIS THEORY OF COLORIMETRY AND SPECTROPHOTOMETRY IS MEANT ONLY AS AN INTRODUCTION TO THE SUBJECT. THIS IS NEITHER EXHAUSTIVE NOR COMPLETE.

Reference: G.H. JEFFERY, J.BASSETT, J.MENDHAM and R.C.DENNEY Vogel's Text book of Quantitative Chemical Analysis, 5th Edition LONGMAN SCIENTIFIC & TECHNICAL, LONGMAN GROUP UK LTD., HARLOW, 1989.

SL 210 ANNEXURE-C

C-1

DOUBLE BEAM UV-VIS SPECTROPHOTOMETER SL 210 SALIENT FEATURES AND BENEFITS

Smallest form factor double beam spectrophotometer with a high-resolution bandwidth

Comes with automatic wavelength calibration, programmable wavelength for lamp

changeover

Lamp selection enables conserving the life of the two lamps, deuterium and tungsten

Menu driven microprocessor based software makes the stand-alone unit user

friendly, the feature enhanced by the soft touch keypad and display.

Stand alone unit offers an advanced technology with data processing features for scanning discrete wavelength, concentration determination by standards and e-factor method, time scan with minimum time interval of 1.0 sec, auto-zero facility, self check and self diagnostic modes.

Data in the form of graph and table can be printed directly onto a printer.

PC compatible through USB

Multitasking user friendly PC software provides the analysis to run all the features

available in the stand alone unit and additionally has techniques to view, analyze and display upto 4th order derivative spectra, overlay of maximum ten spectra, peak picking facilities, % concentration mode, auto-zero facility, spectra zooming, smoothening, data saving and printer

Software is 21 CFR Part 11 compliant with password method protection; Instrument

Log Book etc. cGLP compliance is addressed.

SL 210 ANNEXURE-C

C-2

SPECIFICATIONS PARAMETERS

SPECTRAL Range 190 to 1100 nm Bandwidth 1.8nm Accuracy 0.5 nm Repeatability 0.2nm Readability 0.1nm PHOTOMETRIC System Double beam optics Range 3.000Abs Accuracy 0.005Abs at 1.0Abs 0.010Abs at 1.5Abs Repeatability 0.002Abs at 1.0Abs Stability (Baseline) 0.003Abs/hr. after 2hour warm-up Stray Light < 0.05%T at 220nm with Nal 10g/L Readability %T 0.01 Abs 0.0001 LIGHT SOURCE Deuterium Lamp (D2) & Tungsten (W) Halogen Lamp MONOCHROMATOR Concave holographic grating with 1200 lines/mm DETECTOR Photo Diode CONTROL Microprocessor and Microcontroller (Computer-Optional) DATA PRESENTATION Display 4 line 20 character Dot matrix backlight LCD module Hard Copy On Printer (Centronics) DATA PROCESSING Microprocessor Mode Spectrum, Discrete l, Concentration, e-factor, Time Scan

and Auto zero PC Mode Spectrum, Overlay, Time Scan, Discrete l, Ratiometric,

Concentration, e-factor, Upto 4th order derivative, Peak Picking, Spectra Zooming, Data saving, Retrieving and Printing

PC CONNECTIVITY USB Interface SAMPLE ATTACHMENT Standard Fixed 10mm path holders for Reference & Sample Optional Fixed multiple cell holders for Reference & Sample

accommodating 10 to 100mm cuvettes Fixed cell holder for Reference & 6 position motorized 10mm path cell holder for sample Fixed cell holder to accommodate microcuvettes

SL 210 ANNEXURE-C

C-3

POWER REQUIREMENT 90-260 V, (50-60) Hz, 1f, Max.170VA PHYSICAL DIMENSIONS Size (WXDXH) 500 x 375 x 180 Weight in kg Approx. 15 ACCESSORIES SUPPLIED With Instrument 10mm path length cuvettes At extra cost PC, UV-VIS spectra Treats Software, Multiple path length

cuvette holder, 10, 20, 40, 50 & 100mm path length cuvettes Microcuvettes and Printer

APPLICATIONS

This Instrument finds place for any spectrophotometric or colorimetric measurement where applications require very high-resolution bandwidth and cGLP compliance is mandatory.

Micronutrients like N, P, K, S, Ca, Mg, Zn, Cu, B, Mo, etc., in Agricultural soil, Plants

etc.

Organic compounds in Biological matter

Glucose, Fructose, Carbohydrates, Proteins etc., in Foods.

Edible dyes, alcohols etc., in Beverages

Purity of constituents in Pharmaceuticals

Toxic elements like Cadmium, Lead, Mercury and Arsenic etc., in Effluents.

Constituents in compositions used in Metallurgy, Fertilizer, Pesticide, Chemical, Petro Chemical, Steel, Cement, Glass & Other Industries.

Life science applications

SL 210 ANNEXURE-E

E 1

CARE & MAINTENANCE OF CELLS

(CUVETTES OR PRECISION OPTICAL CELLS)

1. CARE & MAINTENANCE OF CELLS 1.1 GENERAL Use cells-appropriate to the spectral region of interest & application envisaged and

free from scratches, etch marks & other physical defects arising due to improper handling - in perfectly clean condition, to make full use of the accuracy and sensitivity of the electro-optical analytical instruments in which they are being employed.

1.2 CHOICE OF CELLS For measurements in the visible region use cells of special optical glass. For measurements in the Ultra-Violet region of less than 360 nm wavelength use

quartz cells. For measurements in the Ultra-Violet region of less than 220 nm wavelength, only

certain varieties of silica cells are satisfactory, such as those marketed under trade names Spectrosil, Supersil, Ultrasil, supersil, etc.

1.3 CARE OF CELLS DURING USE 1.3.1 Always clean the cells thoroughly and rinse at least once with a portion of the sample,

before filling the sample, for use.

1.3.2 Always wipe the exposed surfaces of the cells dry and free from lint & finger prints, using lintless cloth or soft tissue paper.

1.3.3 Any glass (including vitreous silica) surface can rapidly absorb contamination. Gross

contamination can occur from solvent evaporation, dust or grease. Such films or dust particles decrease the transmission and may contaminate the sample. For example, cells left standing on a laboratory bench for one hour show a drop in transmission up to 0.5%. It is important, therefore, to clean cells immediately after use and prior to use.

1.3.4 While testing proteins or similar organic substances, rinse cells frequently to prevent

sample build-up. Never allow such samples to dry within the cells. 1.3.5 Immediately after use empty and rinse the cells with the solvent, clean with a mild

cleaning agent and store in distilled water, for short time storage. Do not leave solutions, particularly strong alkalis, in the cells for periods longer

than necessary.

SL 210 ANNEXURE-E

E 2

1.3.6 Always handle wet cells using tongs or tweezers, the ends of which are covered by soft, inert material like rubber or PTFE and grasp the cells only on the ground non-optical surfaces. The use of clean cotton gloves when handling dry cells prevents perspiration from fingers spreading to the polished faces of the cells.

NOTE

Ensure no bubbles cling to the inner surfaces of the cells. Finger marks/ prints absorb very strongly light in UV region.

1.3.7 Never use a brush or any instrument, which might scratch the optical surfaces.

Scratches and etching do not necessarily render a cell useless but can reduce transmission and cause light scattering. Always remove solid contamination by wet cleaning method like ultrasonic agitation and never by mechanical means.

1.3.8 Keep the cells in their original cases, with their etched surfaces on the top, when not

in use, for long time. 1.4 CLEANING METHODS 1.4.1 Cleaning procedures vary with the type of cell to be cleaned, accuracy required and

type of contamination occurring from previous use. Use a cleaning method simplest of the ones indicated below appropriate to the application.

1.4.2 A number of cleaning methods are listed in increasing order of severity. Whichever

cleaning procedure is adopted, it is important that, once cleaning has commenced, do not allow the surfaces to dry until the cleaning procedure has been completed.

1.4.3 Mild cleaning methods 1.4.3.1 Method-1 Boil the cells for 15 minutes in a 2% solution of a detergent and follow it with

adequate rinsing in distilled water and Ethanol before drying. 1.4.3.2 Method-2 Take 1 gram of Potassium Dichromate, add a little distilled water and very slowly add

approximately 100 ml of concentrated H2SO4. Keep the cells in this acidic solution for a maximum period of 12 hours. Wash the cells thoroughly with distilled water before use.

NOTE

Cells may get damaged if excess Fluoride is present in the water that is added to Potassium Dichromate.

SL 210 ANNEXURE-E

E 3

1.4.3.3 Method - 3

Immerse the cells and leave overnight in freshly prepared Chromic Acid and rinse thoroughly with distilled water before use.

NOTE

Potassium Dichromate dissolved in concentrated Nitric Acid is preferred to a Chromic - Sulphuric mixture because of its greater oxidizing capacity and mobility.

1.4.3.4 Method-4 Add 30 ml concentrated Hydrochloric Acid to 10 ml concentrated Nitric Acid (Acqua

Regia). Fill the cells with the mixture and allow to stand for 5 to 10 minutes. Thoroughly wash the cells with distilled water before use. Repeat the procedure if necessary.

NOTE

These methods are generally adequate for cells, which are to be used in the visible and infrared spectral regions. Cells, which are to be used in the ultra-violet region, may not be adequately cleaned by these methods.

1.4.4 General method for cleaning cells used in the Ultra-Violet region 1.4.4.1 Immerse cells in concentrated Nitric Acid and agitate either ultrasonically or by

boiling for 10 to 15 minutes. Rinse repeatedly with distilled water followed by rinse in absolute Ethanol and dry.

NOTE

Correct application of this method should give transmission variations of less than 5%.

1.4.4.2 Cells cleaned by this method normally require matching to give sets of cells with a

transmission variation of less than 1.5% at 240 nm.

NOTE B.S. 3875: 1965 requires that matched type 1 cells show a transmission variation of less than 1.5% at 240 nm. Cells cleaned by this procedure may vary in transmission due to different degrees of cleanliness but not because of material variations.

SL 210 ANNEXURE-E

E 4

1.4.5 Method for cleaning cells to ensure reproducible Ultra-Violet transmission 1.4.5.1 Synthetic fused silica cells like Spectrosil do not exhibit the variations in

transmission shown by other forms of vitreous silica. Such cells will match automatically, without selection.

NOTE

This inherent uniformity of transmission will be lost if cells with transmitting plates of Spectrosil are not scrupulously clean.

1.4.5.2 Method 1.4.5.2.1 Remove all traces of liquid previously used in the cells, wash in water, agitate in

concentrated Nitric Acid (boiling or ultrasonic bath) and subsequently rinse in distilled water.

1.4.5.2.2 Immerse the cells in hot (80 to 90oC) saturated solution of Tri-Sodium

Orthophosphate for 10 minutes, with repeated decantation. 1.4.5.2.3 Rinse in several changes of hot distilled water. 1.4.5.2.4 Immerse, with repeated decantation, in boiling distilled water for 10 minutes. 1.4.5.2.5 Rinse in cool reagent grade Ethanol. 1.4.5.2.6 Rinse thoroughly, at least twice, in hot, freshly distilled Ethanol. Allow to dry in

clean dust-free air.

NOTE Vitreous silica or inert plastic (e.g. PTFE) should be used for operation of steps 1.4.5.2.4 onwards since glass vessels may introduce contamination.

This is only one of a number of possible methods and is not recommended for repeated use since slight etching of the transmitting plates can occur with prolonged immersions. However it demonstrates effectively the results of efficient cleaning.

This method is capable of cleaning Spectrosil cells to a degree such that the variation in transmission at 240 nm will be less than 1%.

SL 210 ANNEXURE-E

E 5

1.4.6 Special methods 1.4.6.1 Method - 1 If stubborn organic contamination is present and it is not possible to obtain the

required level of transmission by operation of steps of 1.4.5, use hot Perchloric Acid instead of Orthophosphate in 1.4.5.2.2.

CAUTION

Perchloric acid is a dangerous liquid and due care should be exercised in its use.

1.4.6.2 Method - 2 1.4.6.2.1 Excellent results can be obtained by cleaning the transmitting plates of demountable

cells using the Ethanol-Nitric acid reaction. Transmission variations of less than 0.5% at 240 bn can be achieved for Spectrosil plates using this method carried out in a fume cupboard.

1.4.6.2.2 Place the plates in a shallow, curved-bottomed Vitreosil or porcelain dish with not

more than 0.5 ml, Ethanol in a fume cupboard. Add about five times this volume of cold concentrated Nitric Acid and cover the dish immediately with a large, inverted beaker. Close the fume cupboard. After the reaction has subsided carry out the recommended rinsing and drying procedure.

CAUTION

This method is as DANGEROUS as it is effective. Any Ethanol Nitric Acid mixture reacts violently, immediately or after a delay, producing a large volume of noxious fumes. Therefore it should only be used if the dangers involved are fully understood.

NOTE

To obtain the maximum benefit from any cleaning method, the final stages of rinsing and drying are critical.

Copious rinsing in distilled water is recommended followed by a final rinse in absolute Ethanol or other pure readily volatile and miscible with water solvent, before drying. Where possible, drying should be accelerated by gentle heat or vacuum. Drying the cells with a jet of air is not recommended but may be used if adequate precautions are taken (e.g. using appropriate filters, drying agents, etc.).

SL 210 ANNEXURE-E

E 6

CAUTION These cleaning methods can safely be applied to cells of all-fused construction. Cemented cells and those incorporating intermediate glasses in their construction may be damaged and may even disintegrate using some of the processes described.

1.4.7 Checking the cleanliness of cells

A clean cell filled with distilled water should have the following absorbance percentage Transmission values relative to air.

Cell type Wavelength (nm) Absorbance

(Abs.) %T

Optical Glass 650 < 0.035 > 90 Silica 240 < 0.093 > 80

SL 210 ANNEXURE-F

F-1

NOTE

DISPOSE THE MATERIAL IN THE SAFE ENVIRONMENT AFTER USE OR HANDOVER TO OUR NEAREST SALES OFFICES

INSTRUMENT WARRANTY CARD

Instrument Model Sl.No.

Customers Ref. No. Date

Elico Delivery Note No. Date

PLEASE PRINT This Instrument is warranted against defects in material and workmanship for a period of one year from the date of shipment.

For ELICO LIMITED

1. Please fill in, separate at perforation and mail the self addressed Instrument Registration Card within 21 days of the date of Delivery Note.

2. Please inform any change in your address.

3. This warranty shall not apply to fuses, lamps, batteries, glass & fragile items and to defects resulting from improper location, installation, operation or battery discharge.

4. This warranty becomes void if the Instrument is serviced by Agencies other than ELICO Sales & Support Centres or resold by the original customer.

5. Suggestions for improvement of the instrument and Documentation provided will be highly appreciated.

INSTRUMENT WARRANTY CARD

Instrument: Model: Sl.No.:

Customers Ref. No.: Date:

Elico Delivery Note No.: Date:

Signature:

Name:

Customer Address:

Date:

Signature:

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Branch/Dealer Address:

Date: PLEASE PRINT

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NIR SPECTROPHOTOMETER ION ANALYSER

DIODE ARRAY SPECTROPHOTOMETER pH METERS / ANALYSER

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CERTIFICATE

ELICO LIMITED certifies that this instrument meets its published specifications at the time of shipment from the factory after due to calibration.

WARRANTY

This instrument is warranted against defects in material and workmanship for a period of ONE YEAR from the date of shipment (except for those components, whose warranty shall be as indicated specifically in the manual). During the warranty period ELICO LIMITED will at its option, either repair or replace products which prove to be defect

SL210 Users Manual

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