PROTOCOL FOR MOUSE PLASMA - Diapharma...PROTOCOL FOR MOUSE PLASMA TECHNOTHROMBIN TGA WITH MOUSE...
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MTGA01_001 06/2006 PROTOCOL FOR MOUSE PLASMA TECHNOTHROMBIN TGA WITH MOUSE PLASMA - INFLUENCE OF USING DIFFERENT PLASMA DILUTIONS 1:2 diluted mouse plasma: TECHNOTHROMBIN ® TGA substrate(SUB) 8F52000 Sample : Thomas Perkmann, MD 1:1 dilution of mouse plasma + trigger C high + TC® substrate 0 5000 10000 15000 20000 25000 30000 35000 1 4 7 10 13 16 19 22 25 28 31 34 37 40 43 46 49 52 55 58 min RFU/min 1:1 dilution of mouse plasma + trigger C high + TC® substrate pooled normal plasma from C57/Bl6 mice; the plasma was diluted 1:2 with TGA-buffer and 40 μl of the dilution was used for the TGA as described for human plasma samples. Reaction mix : 40 μl plasma or plasma-dilution 10 μl trigger C high (TC®) 50 μl substrate (TC®) A 1:2 dilution of the mouse plasma is recommended to obtain measurable thrombin generation! Undiluted mouse plasma: TECHNOTHROMBIN ® TGA substrate(SUB) 8F52000 undiluted mouse plasma + trigger C high + TC® substrate 0 35000 undiluted mouse plasma + trigger C high + TC® substrate 30000 10000 5000 15000 20000 25000 1 4 7 10 13 16 19 22 25 28 31 34 37 40 43 46 49 52 55 58 min RFU/min Thomas Perkmann, MD
Transcript of PROTOCOL FOR MOUSE PLASMA - Diapharma...PROTOCOL FOR MOUSE PLASMA TECHNOTHROMBIN TGA WITH MOUSE...
MTGA01_001 06/2006
PROTOCOL FOR MOUSE PLASMA
TECHNOTHROMBIN TGA WITH MOUSE PLASMA - INFLUENCE OF USING DIFFERENT PLASMA DILUTIONS
1:2 diluted mouse plasma: TECHNOTHROMBIN® TGA substrate(SUB) 8F52000
Sample:
Thomas Perkmann, MD
1:1 dilution of mouse plasma + trigger C high + TC® substrate
0
5000
10000
15000
20000
25000
30000
35000
1 4 7 10 13 16 19 22 25 28 31 34 37 40 43 46 49 52 55 58
min
RF
U/
min
1:1 dilution of mouseplasma + trigger Chigh + TC® substrate
pooled normal plasma from C57/Bl6 mice; the plasma was diluted 1:2 with TGA-buffer and 40 µl of the dilution was used for the TGA as described for human plasma samples.
Reaction mix: 40 µl plasma or plasma-dilution 10 µl trigger C high (TC®) 50 µl substrate (TC®)
A 1:2 dilution of the mouse plasma is recommended to obtain measurable thrombin generation!
Undiluted mouse plasma: TECHNOTHROMBIN® TGA substrate(SUB) 8F52000
undiluted mouse plasma + trigger C high + TC® substrate
0
35000
undiluted mouse plasma +trigger C high + TC®substrate
30000
10000
5000
15000
20000
25000
1 4 7 10 13 16 19 22 25 28 31 34 37 40 43 46 49 52 55 58
min
RF
U/
min
Thomas Perkmann, MD
tfister
Typewritten Text
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