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Transcript of Protein Synthesis Transcription Translation ...
Protein Synthesis
• Transcription
• Translation
http://www-class.unl.edu/biochem/gp2/m_biology/animation/gene/gene_a1.html
Animation – overview of transcription/translation
http://www-class.unl.edu/biochem/gp2/m_biology/animation/gene/gene_a2.htmlTranscription - detail
http://www-class.unl.edu/biochem/gp2/m_biology/animation/gene/gene_a3.htmlTranslation - detail
Types of RNA
• mRNA
• tRNA
• rRNA
3 steps of transcription•Promoter Region
•RNA Polymerase
•Primary Transcript is made from the coding strand of DNA
•Terminator Region
Transcription Animation
RNA Processing
mRNA has a short life span
•A cap is added
•A poly-A tail is added
Protect enzymes from breaking down mRNA in the cytoplasm
•Introns
•Exons
Splicing is the process of removing introns and rejoining cut ends
The Genetic Code (pg 237)
Combine DNA nucleotides to code 20 different “words” (amino acids)
The Genetic Code
Translation
http://www-class.unl.edu/biochem/gp2/m_biology/animation/gene/gene_a3.htmlTranslation Animation – detailed:
Making a Protein
1. DNA serves as a template in for making RNA in the nucleus
2. RNA goes to the cytoplasm
3. RNA attaches to the ribosome
4. tRNA contains an anticodon that is complementary to the codon of the mRNA
5. Amino acids are bonded together to make a polypeptide
THEDOGBITTHECATTHEDOGBITTHECAR
POINT MUTATION:
A change in a single base pair in DNA
THE DOG BIT THE CATTHE DOG BIT THE CAR
Genetic Mutations
Genetic Mutations
THEDOGBITTHECATTHE DOB ITT HEC AT
If a single base is added or deleted from DNA, this is a FRAMESHIFT MUTATION, because it shifts the reading of codons by one base.
A different sequence of codons and amino acids results.
Genetic Mutations
Genetic Mutations
DNA BASE SEQUENCES:
Normal Hemoglobin GGG CTT CTT TTT
Sickled Hemoglobin GGG CAT CTT TTT
(Sickle-cell anemia)Point Mutation or Frameshift Mutation?
Sickle Cell Anemia
POINT MUTATION OR FRAMESHIFT MUTATION?
The 20 Amino AcidsGeneral Structure
(Variable or R group)
CODON
BINGO!
VIRUSES
• Living or Not?• No cells• Tiny particles• Replicate• Evolve• Do not carry out own
metabolism• Do not respond to
environment• Different structures
http://www.newtown.k12.ct.us/~royalk/viruses.htm
How do viruses replicate?
• Host cell enzymes replicate viral DNA
• Viral genes transcribed and translated on host ribosomes
• Proteins reassemble into virus particles
• The cells lyses (splits)when there are many viruses in the host cell; they infect other cells
Lytic Infections Lysogenic Infections
• Viral DNA inserts into cellular DNA• Viral DNA is therefore copied when
the host cell replicates• No production of new viruses• Stress to host can activate cell to
enter into lytic cyle (example: starvation)
Protein structure• Primary• Secondary• Tertiary• Quaternary
Chromosome Structure
DNA helix
nucleosomes
30 nm solenoid
30 nm solenoid
chromatin loops
scaffold-associated condensed loops
Chromatin
30 nm solenoid
chromatin loops
scaffold-associated condensed loops
metaphase chromosome
scaffold-associated condensed loops
How nucleosomes affect transcription
DNA wrapped around
histone proteinstwo H2Atwo H2Btwo H3two H4
nucleosome particle in a 30nm fiber
histone octamer
How nucleosomes affect transcription
• (+)-charged lysines of histone proteins interact with (-)-charged phosphates of DNA
+
+
+
+
• histone acetylation is correlated with transcriptional activity
• reversible acetylation of lysines neutralizes charges
• DNA decondenses from histone octamer
• DNA is tightly wrapped around histone octamer
Ac
Ac Ac
Ac
+
N-O-C-CH3
O||
|H
lysine
acetylated lysine
Transcription activators can promote histone acetylation
TATAHRE
nuclear hormone receptors
TATA
co-Activator(s)
coA HAT
Histone Acetyl Transferase
TFIID
histone acetylation
Copyright © The National Human Genome Research Institute