7/30/2019 Proposal Research Gibran NEWEdited

1/15

SABIRAN

P 63097

Supervisor : Dr. NORHANA ARSAD

Department of Electric, Electronic and System Engineering,Faculty of Engineering and Built Environment,

The National University of Malaysia.

7/30/2019 Proposal Research Gibran NEWEdited

2/15

OUTLINE Introduction

I. The problem statementII. Objectives

Literature review Methodology

I. Experimental set upII. Data collection plansIII. Proposed analysis of dataIV. Experimental setup

Expected Result

Conclusion In Progress Appendices

I. TimelineII. List of references

III. instruments

7/30/2019 Proposal Research Gibran NEWEdited

3/15

INTRODUCTION Carcinogen is a type of ?? before becomes cancerwhere

the level of nitrite for a cancer patient is higher thannormal person.

Therefore, detection and monitoring of nitrite areimportant as an initial action to make sure this terminaldisease can be treated earlier.

Nitrite is ubiquitous within environmental, food,industrial and physiological system. (Moorcroft et al.

2001) The toxicity of nitrite is primary due to the fact that it can

react with secondary or tertiary amines present in humanbody to form nitrosamines, which are known to be

carcinogens and nitrite can react with iron(III) of

7/30/2019 Proposal Research Gibran NEWEdited

4/15

Figure 1 Salivary concentrations of total nitric oxide (NO),

nitrates (NO3), and nitrites (NO2) in healthy and oralsquamous cell carcinoma (OSCC).Source:Bahar et al 2007, Gokul. 2012

concentration of nitrite in human saliva can be as

biomarker for screening of oral cancer. ()

7/30/2019 Proposal Research Gibran NEWEdited

5/15

I. The problem statementComplicated and larger cell required for nitritedetermination

-fiber optic SMA Z-flow cell can provide simple and lowvolume sample for optical nitrite determination inmedical and life science application.

to design and develop an optical sensor for tracing nitrite. to analyse the fiber optic SMA Z-flow cell for nitrite

determination. to study types of injection effect for optical absorbance. to develop non-invasive method for cancer screening test.

II. Objectives

7/30/2019 Proposal Research Gibran NEWEdited

6/15

LITERATURE REVIEWSpectrophotometry is a quantitative measurement of the reflectionor transmission properties of a material as a function of wavelength

in UV/VIS range. The ratio of the intensity of light entering thesample (I0) to that exiting the sample (I) at a particular wavelengthis defined as the transmittance (T) or (%T), absorbance (A)(Upstone 2006)

The linear relationship betweenconcentration and absorbance is stated

in Beer-Lambert law. Absorptivity (),

Concentration (c) and path lengt (l)

7/30/2019 Proposal Research Gibran NEWEdited

7/15

METHODOLOGY

There are three experimental configurations that

purposed in the research :1. Stopped-flow injection2. Continuous Injection3. Reverse flow injection

1. Stopped Flow Injection

7/30/2019 Proposal Research Gibran NEWEdited

8/152. Continuous Injection

3. Reverse Flow Injection

7/30/2019 Proposal Research Gibran NEWEdited

9/15

I. Experimental Setup

1. Light source P in

broadband laser LED

3. Z-flow injection Flow rate Temperature Time Colorimetric

Acidiccondition 4. Data acquisitiono P outo Absorbanceo Transmittanceo shiftingo

Attenuation

2. Fiber optics Silica

POF Diameter size Length

7/30/2019 Proposal Research Gibran NEWEdited

10/15

II. Data collection plansData acquisition by using SpectraSuite software,a) Absorbance of allwavelengths for standard solution.

b) Using double beam spectrophotometer.c) Absorbance in every 5 minutes for 50 minutes persample.

d) Absorbance under acidic condition.e) Transmittance value.

f) Absorbance and transmittance under modifiedcolorimetric.

III. Proposed analysis of dataa) Mathematical error analysis for absorbance,

transmittance and concentration.b) Time response analysis.c) Sensitivity analysis.

d) Uncertain analysis.

7/30/2019 Proposal Research Gibran NEWEdited

11/15

EXPECTED RESULT

Simple design of nitrite detection system

Better sensitivity optical nitrite sensor

Low and large volume sample of the sensor

Nitrite sensor in medical application

CONCLUSIONThe goal this study is to investigate of fiber optic SMA Z-flow cell foroptical nitrite sensor to achieve better performance in low volumesample in medical and life science application.This study is to investigate the fiber optic SMA Z-flow cell with

various methods in detecting nitrate for cancer screening application.

7/30/2019 Proposal Research Gibran NEWEdited

12/15

In Progress Nitrite detection for cancer screening application

using spectrophotometric- submitting to Journal ofBiomedical

Study of Nitrite Optical Sensor by Comparinginjection methods for cancer screening application-submitting to

7/30/2019 Proposal Research Gibran NEWEdited

13/15

I. TimelineAPPENDICES

Title:SPECTROPHOTOMETRIC USING FIBER OPTIC SMA Z-FLOW CELL FOR NITRITE DETECTION

No Task

Year 1 (2011/2012) Year 2 (2012/2013)

9 11 1 3 5 7 9 11 1 3 5 7

10 12 2 4 6 8 10 12 2 4 6 8

1Literature review about the theory, types of

nitrite optical sensor2 Define problem statement

3 Design experimental set up

4 Experimental 1 (stopped flow injection)

5

Experimental 2 (Continuous Injection)6 Experimental 3 (Reverse flow injection)

7 Data analysis

8 Writing up the thesis

9 Thesis submission

7/30/2019 Proposal Research Gibran NEWEdited

14/15

II. List of references

Bahar, G., Feinmesser, R., Shpitzer, T., Popovtzer, A. & Nagler, R. M. 2007.Salivary Analysis in Oral Cancer Patients. Cancer 109(1): 54-59.

Burakham, R., Oshima, M., Grudpan, K. & Motomizu, S. 2004. SimpleFlow-Injection System for the Simultaneous Determination of Nitrite andNitrate in Water Samples. Talanta 64(5): 1259-1265.

Gokul, S. 2012. Salivary Diagnostics in Oral Cancer. Dlm. SalivaryDiagnostics in Oral Cancer. http://www.intechopen.com/books/oral-cancer/salivary-diagnostics-in-oral-cancer

Moorcroft, M. J., Davis, J. & Compton, R. G. 2001. Detection andDetermination of Nitrate and Nitrite: A Review. Talanta 54(5): 785-803.

Upstone, S. L. 2006. Ultraviolet/Visible Light AbsorptionSpectrophotometry in Clinical Chemistry. Encyclopedia of AnalyticalChemistry

http://www.intechopen.com/books/oral-cancer/salivary-diagnostics-in-oral-cancerhttp://www.intechopen.com/books/oral-cancer/salivary-diagnostics-in-oral-cancerhttp://www.intechopen.com/books/oral-cancer/salivary-diagnostics-in-oral-cancerhttp://www.intechopen.com/books/oral-cancer/salivary-diagnostics-in-oral-cancerhttp://www.intechopen.com/books/oral-cancer/salivary-diagnostics-in-oral-cancerhttp://www.intechopen.com/books/oral-cancer/salivary-diagnostics-in-oral-cancerhttp://www.intechopen.com/books/oral-cancer/salivary-diagnostics-in-oral-cancerhttp://www.intechopen.com/books/oral-cancer/salivary-diagnostics-in-oral-cancerhttp://www.intechopen.com/books/oral-cancer/salivary-diagnostics-in-oral-cancerhttp://www.intechopen.com/books/oral-cancer/salivary-diagnostics-in-oral-cancerhttp://www.intechopen.com/books/oral-cancer/salivary-diagnostics-in-oral-cancerhttp://www.intechopen.com/books/oral-cancer/salivary-diagnostics-in-oral-cancerhttp://www.intechopen.com/books/oral-cancer/salivary-diagnostics-in-oral-cancerhttp://www.intechopen.com/books/oral-cancer/salivary-diagnostics-in-oral-cancerhttp://www.intechopen.com/books/oral-cancer/salivary-diagnostics-in-oral-cancerhttp://www.intechopen.com/books/oral-cancer/salivary-diagnostics-in-oral-cancerhttp://www.intechopen.com/books/oral-cancer/salivary-diagnostics-in-oral-cancerhttp://www.intechopen.com/books/oral-cancer/salivary-diagnostics-in-oral-cancerhttp://www.intechopen.com/books/oral-cancer/salivary-diagnostics-in-oral-cancer

7/30/2019 Proposal Research Gibran NEWEdited

15/15

THANK YOU