NON-HUMAN PRIMATE CELL ISOLATION · Typical EasySep™ Non-Human Primate T Cell Isolation Profile...
2
NON-HUMAN PRIMATE CELL ISOLATION Use EasySep ™ for a Smarter Way to Isolate Cells Scientists Helping Scientists ™ | WWW.STEMCELL.COM Non-human primate cell isolation has never been this fast and easy. Isolate non-human primate cells in as little as 20 minutes with EasySep ™ , a powerful immunomagnetic cell isolation platform. Isolated cells are highly purified, untouched, and ready for immediate downstream use. Learn more at www.EasySep.com. Custom Cell Separation Kits Are you trying to isolate other non-human primate cell types? Explore our custom cell separation kits to obtain non-human primate cells from your sample. • EasySep ™ Non-Human Primate Custom Positive Selection Kit (Catalog #18809) • EasySep ™ Non-Human Primate Custom Enrichment Kit (Catalog #19809) Choose the Cell Isolation Platform That Best Fits Your Needs Why Use EasySep ™ to Isolate Non-Human Primate Cells? FAST AND EASY. Isolate immune cells in as little as 20 minutes from peripheral blood mononuclear cells (PBMCs). COLUMN-FREE. With no columns or washes, the simple EasySep ™ pour-off is gentle on your cells. HIGH PURITY. Obtain highly purified cells that are immediately ready for downstream functional studies. EasySep ™ Kits for Non-Human Primate Cell Isolation Cell Type Catalog # T Cell 19581* CD4 + T Cell 19582* CD8 + T Cell 19583* *These kits have been verified for use with PBMCs from rhesus and cynomolgus macaques. EasySep ™ Manual Cell Separation RoboSep-S ™ & RoboSep ™ -16 Fully Automated Cell Separation
Transcript of NON-HUMAN PRIMATE CELL ISOLATION · Typical EasySep™ Non-Human Primate T Cell Isolation Profile...
NON-HUMAN PRIMATE CELL ISOLATIONUse EasySep™ for a Smarter Way to Isolate Cells
Scientists Helping Scientists™ | WWW.STEMCELL.COM
Non-human primate cell isolation has never been this fast and easy. Isolate non-human primate cells in as little as 20 minutes with EasySep™, a powerful immunomagnetic cell isolation platform. Isolated cells are highly purified, untouched, and ready for immediate downstream use.
Learn more at www.EasySep.com.
Custom Cell Separation KitsAre you trying to isolate other non-human primate cell types?
Explore our custom cell separation kits to obtain non-human primate cells from your sample.
• EasySep™ Non-Human Primate Custom Positive Selection Kit (Catalog #18809)
• EasySep™ Non-Human Primate Custom Enrichment Kit (Catalog #19809)
Choose the Cell Isolation Platform That Best Fits Your Needs
Why Use EasySep™ to Isolate Non-Human Primate Cells?
FAST AND EASY. Isolate immune cells in as little as
20 minutes from peripheral blood mononuclear cells (PBMCs).
COLUMN-FREE. With no columns or washes, the simple
EasySep™ pour-off is gentle on your cells.
HIGH PURITY. Obtain highly purified cells that are
immediately ready for downstream functional studies.
EasySep™ Kits for Non-Human Primate Cell Isolation
Cell Type Catalog #
T Cell 19581*
CD4+ T Cell 19582*
CD8+ T Cell 19583*
*These kits have been verified for use with PBMCs from rhesus and cynomolgus macaques.
EasySep™ Manual Cell Separation
RoboSep-S™ & RoboSep™-16 Fully Automated Cell Separation
NON-HUMAN PRIMATE CELL ISOLATION
Copyright © 2019 by STEMCELL Technologies Inc. All rights reserved including graphics and images. STEMCELL Technologies & Design, STEMCELL Shield Design, Scientists Helping Scientists, EasySep, and RoboSep
are trademarks of STEMCELL Technologies Canada Inc. All other trademarks are the property of their respective holders. While STEMCELL has made all reasonable efforts to ensure that the information provided by
STEMCELL and its suppliers is correct, it makes no warranties or representations as to the accuracy or completeness of such information.
STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR
THERAPEUTIC USES UNLESS OTHERWISE STATED.
TOLL FREE PHONE 1 800 667 0322 • PHONE +1 604 877 0713 • [email protected] • [email protected]
FOR GLOBAL CONTACT DETAILS VISIT WWW.STEMCELL.COM DOCUMENT #29943 VERSION 3.0.0 SEPT 2019
How Does EasySep™ Work?
Typical EasySep™ Non-Human Primate Cell Isolation Protocol (Negative Selection)
Highly Purified Cells
EasySe
pTM
EasySe
pTM
1
Add EasySep™ Isolation
Cocktail to single-cell
suspension.
2
Add EasySep™
magnetic particlesto cell suspension.
4
Pour off desired cells
into a new tube.
3
Place tube in EasySep™
Magnet and incubate
5 minutes*.
CellSuspension
Incubate10 minutes
Incubate5 minutes
EasySep TM
EasySepTM
Typical EasySep™ Non-Human Primate T Cell Isolation Profile
In the above example, starting with rhesus macaque PBMCs, the T cell content (within the CD45+ gate) of start and final isolated fractions are 55.7% and 93.9%, respectively, and the ratio of CD4+ to CD8+ cells is maintained.
For a complete list of EasySep™ cell isolation kits, please visit www.stemcell.com
EasySe
pTM
EasySe
pTM
1
Add EasySep™ Isolation
Cocktail to single-cell
suspension.
2
Add EasySep™
magnetic particlesto cell suspension.
4
Pour off desired cells
into a new tube.
3
Place tube in EasySep™
Magnet and incubate
5 minutes*.
CellSuspension
Incubate10 minutes
Incubate5 minutes
EasySep TM
EasySepTM
EasySe
pTM
EasySe
pTM
1
Add EasySep™ Isolation
Cocktail to single-cell
suspension.
2
Add EasySep™
magnetic particlesto cell suspension.
4
Pour off desired cells
into a new tube.
3
Place tube in EasySep™
Magnet and incubate
5 minutes*.
CellSuspension
Incubate10 minutes
Incubate5 minutes
EasySep TM
EasySepTM
EasySe
pTM
EasySe
pTM
1
Add EasySep™ Isolation
Cocktail to single-cell
suspension.
2
Add EasySep™
magnetic particlesto cell suspension.
4
Pour off desired cells
into a new tube.
3
Place tube in EasySep™
Magnet and incubate
5 minutes*.
CellSuspension
Incubate10 minutes
Incubate5 minutes
EasySep TM
EasySepTM
EasySe
pTM
EasySe
pTM
1
Add EasySep™ Isolation
Cocktail to single-cell
suspension.
2
Add EasySep™
magnetic particlesto cell suspension.
4
Pour off desired cells
into a new tube.
3
Place tube in EasySep™
Magnet and incubate
5 minutes*.
CellSuspension
Incubate10 minutes
Incubate5 minutes
EasySep TM
EasySepTM
EasySe
pTM
EasySe
pTM
1
Add EasySep™ Isolation
Cocktail to single-cell
suspension.
2
Add EasySep™
magnetic particlesto cell suspension.
4
Pour off desired cells
into a new tube.
3
Place tube in EasySep™
Magnet and incubate
5 minutes*.
CellSuspension
Incubate10 minutes
Incubate5 minutes
EasySep TM
EasySepTM
EasySe
pTM
EasySe
pTM
1
Add EasySep™ Isolation
Cocktail to single-cell
suspension.
2
Add EasySep™
magnetic particlesto cell suspension.
4
Pour off desired cells
into a new tube.
3
Place tube in EasySep™
Magnet and incubate
5 minutes*.
CellSuspension
Incubate10 minutes
Incubate5 minutes
EasySep TM
EasySepTM
EasySe
pTM
EasySe
pTM
1
Add EasySep™ Isolation
Cocktail to single-cell
suspension.
2
Add EasySep™
magnetic particlesto cell suspension.
4
Pour off desired cells
into a new tube.
3
Place tube in EasySep™
Magnet and incubate
5 minutes*.
CellSuspension
Incubate10 minutes
Incubate5 minutes
EasySep TM
EasySepTM
EasySe
pTM
EasySe
pTM
1
Add EasySep™ Isolation
Cocktail to single-cell
suspension.
2
Add EasySep™
magnetic particlesto cell suspension.
4
Pour off desired cells
into a new tube.
3
Place tube in EasySep™
Magnet and incubate
5 minutes*.
CellSuspension
Incubate10 minutes
Incubate5 minutes
EasySep TM
EasySepTM
EasySe
pTM
EasySe
pTM
1
Add EasySep™ Isolation
Cocktail to single-cell
suspension.
2
Add EasySep™
magnetic particlesto cell suspension.
4
Pour off desired cells
into a new tube.
3
Place tube in EasySep™
Magnet and incubate
5 minutes*.
CellSuspension
Incubate10 minutes
Incubate5 minutes
EasySep TM
EasySepTM
EasySe
pTM
EasySe
pTM
1
Add EasySep™ Isolation
Cocktail to single-cell
suspension.
2
Add EasySep™
magnetic particlesto cell suspension.
4
Pour off desired cells
into a new tube.
3
Place tube in EasySep™
Magnet and incubate
5 minutes*.
CellSuspension
Incubate10 minutes
Incubate5 minutes
EasySep TM
EasySepTM
*Time for each kit will vary depending on the exact isolation protocol and magnet used.
