Methods in Enzymology

Volume 75

Cumulative Subject IndexVolumes XXXI, XXXII, XXXIV-LX

Martha G. Dennis

Edward A . Dennis

VOLUME XXXI

BIOMEMBRANES (PART A)

Section I . Multiple Fractions from a Single TissueSection II . Isolation of Purified Subcellular Fractions and Derived Mem-

branes (from Mammalian Tissue Excluding Nerve)A. Plasma MembraneB. Golgi ComplexC. Rough and Smooth Microsome sD. NucleiE. Mitochondri aF. Lysosome s

G. Peroxisomes

H. Secretory Granule sI. Specialized Cell Fractions

Section III . Subcellular Fractions Derived from Nerve TissueSection IV. Cell Fractions Derived from Plant Tissue

A. General AspectsB. Specific Organelles and Derived Component s

Section V. Preparations Derived from Unicellular OrganismsSection VI . General Methodology

VOLUME XXXII

BIOMEMBRANES (PART B)

Section I. Characterization of Membranes and Membrane ComponentsA. Electron MicroscopyB. Protein Components of MembranesC. Phospholipid Components of Membrane sD. Biophysical Techniques

Section II . Isolation of Selected Membrane ComponentsA. From AnimalsB. From Plant sC. From Unicellular Organism s

Section III . Reconstitution of Membranes or Membrane ComplexesSection IV. Model Membranes

Section V. Isolation and Culture of Cell sA. Basic Methodology for Cell Cultur eB. Isolation of Specific Cell Type sC. Isolation and Culture of Specific Cell Type sD. Membranes and Membrane Variants from Microorganism s

VOLUME XXXI V

AFFINITY TECHNIQUE S

Section I . Introductio nSection II . Coupling Reactions and General MethodologySection III . Attachment of Specific Ligands and Methods for Specific Pro-

teinsA. Coenzymes and Cofactor sB. Sugars and Derivative sC. Amino Acids and PeptidesD. Nucleic Acids, Nucleotides, and Derivative sE. Other Systems

Section IV . Purification of Synthetic Macromolecule sSection V . Purification of ReceptorsSection VI . Immunological Approache s

VOLUME XXXV

LIPIDS (PART B)

Section I. Fatty Acid SynthesisSection II. Fatty Acid Activation and OxidationSection III. HMG-CoA Enzyme sSection IV . HydrolasesSection V . Miscellaneous EnzymesSection VI . General Analytical MethodsSection VII . Synthesis or Preparation of SubstratesSection VIII . Preparation of Single Cell sSection IX. In Vivo and Perfusion Techniques

VOLUME XXXVI

HORMONE ACTION (PART A : STEROID HORMONES)

Section I . Hormone-Binding Proteins and Assays for Steroid Hormone sSection II . Serum-Binding Proteins for Steroid and Thyroid Hormone sSection III . Cytoplasmic Receptors for Steroid Hormones

Section N. Nuclear Receptors for Steroid Hormone sSection V. Purification of Receptor for Steroid Hormone sSection VI. Steroid Hormone Effects on Biochemical Processe sSection VII. Isolation of Biologically Active Metabolites of Steroid an d

Thyroid Hormones

VOLUME XXXVII

HORMONE ACTION (PART B : PEPTIDE HORMONES)

Section I . Hormone AssaysSection II . Hormone ReceptorsSection III. Evaluation of Biological Effects of Hormone sSection IV . Purification and Synthesis of Hormone s

VOLUME XXXVIII

HORMONE ACTION (PART C : CYCLIC NUCLEOTIDES )

Section I . Extraction and Purification of Cyclic NucleotidesSection II . Assay of Cyclic NucleotidesSection III . Biosynthesis of Cyclic NucleotidesSection IV. Degradation of Cyclic NucleotidesSection V. Cyclic Nucleotide-Dependent Protein Kinases and Binding Pro-

teinsSection VI . Synthetic Derivatives of Cyclic Nucleotides and Their Pre-

cursors

VOLUME XXXI X

HORMONE ACTION (PART D : ISOLATED CELLS, TISSUES ,AND ORGAN SYSTEMS)

Section I. KidneySection II . LiverSection III . HeartSection N. Skeletal and Smooth MuscleSection V. Endocrine and Reproductive TissueSection VI . Neural TissueSection VII . Exocrine TissueSection VIII . Vascular TissueSection IX. Unicellular Organism sSection X . Substrate and Ion Fluxes

VOLUME X L

HORMONE ACTION (PART E : NUCLEAR STRUCTURE ANDFUNCTION)

Section I . The Cell Nucleus and Cell DivisionSection II . The Cell Nucleus and Chromatin Protein sSection III. General Methods for Evaluating Hormone Effect s

VOLUME XLI

CARBOHYDRATE METABOLISM (PART B )

Section I . Analytical Method sSection II . Enzyme Assay ProceduresSection III. Preparation of SubstratesSection IV . Oxidation-Reduction EnzymesSection V . Epimerases and Isomerase sSection VI . Miscellaneous Enzymes

VOLUME XLI I

CARBOHYDRATE METABOLISM (PART C )

Section I. KinasesSection II. AldolasesSection III . DehydratasesSection IV. Phosphatase sSection V . MutasesSection VI . Carboxylases and Decarboxylase sSection VII . Glycosidases

VOLUME XLIII

ANTIBIOTIC S

Section I. Methods for the Study of Antibiotic sSection II . Antibiotic Biosynthesis

A. Aminoglycoside AntibioticsB. ß-Lactam Antibiotic sC. ErythromycinD. IndolmycinE. NovobiocinF. Nucleoside Antibiotic sG. PatulinH. Peptide AntibioticsI. Tetracyclines

Section III . Antibiotic Inactivation and ModificationA. Aminoglycoside Antibiotic sB. ß-Lactam AntibioticsC. ChloramphenicolD. ClindamycinE. Nucleoside AntibioticsF. Peptide Antibiotics

VOLUME XLI V

IMMOBILIZED ENZYMES

Section I. General Comment sSection II. Immobilization Technique s

A. Covalent Couplin gB. AdsorptionC. Entrapment and Related Technique sD. AggregationE. MiscellaneousF. Other Techniques and General Classificatio n

Section III . Assay Procedure sSection IV. Characterization by Physical Method sSection V . Characterization by Chemical MethodsSection VI. Kinetics of Immobilized Enzyme sSection VII. Multistep Enzyme SystemsSection VIII . The Application of Immobilized Enzymes to Fundamental

Studies in Biochemistr ySection IX . Application of Immobilized Enzyme s

A. Analytical Are aB. Medical AreaC. Enzyme Engineering (Enzyme Technology)D. Application of Immobilized Enzymes in Organic Chemistry

Section X . Immobilized CoenzymesSection XI . Miscellaneous

VOLUME XLV

PROTEOLYTIC ENZYMES (PART B )

Section I . General AspectsSection II . Blood Clotting Enzyme s

A. Enzymes of Blood CoagulationB. Snake Venom Enzymes with Coagulating Activitie s

Section III . Enzymes of Clot Lysis

Section IV. Kallikreins

Section V. Proteases from Gametes and Developing EmbryosSection VI . Dipeptidase s

Section VII. Endopeptidases

Section VIII. Exopeptidases

A. Aminopeptidases

B. Carboxypeptidases

Section IX. Naturally Occurring Protease InhibitorsA. Specific Inhibitors of Clotting and Lysis in BloodB. Inhibitors from Bacteria

C. Inhibitors from Plant sD. Protease Inhibitors from Various Sources

VOLUME XLV I

AFFINITY LABELING

Section I. General Methodolog ySection II . Specific Procedures for Enzymes, Antibodies, and Other Pro-

teinsA. Proteolytic SystemsB. Nucleotide and Nucleic Acid System sC. Carbohydrate SystemsD. Amino Acid SystemsE. Steroid SystemsF. AntibodiesG. Other ProteinsH. Receptors and Transport Systems

Section III . Specific Procedures for Nucleic Acids and Ribosomal Systems

VOLUME XLVII

ENZYME STRUCTURE (PART E)

Section I . Amino Acid Analysi sSection II . End Group Method sSection III . Chain SeparationSection IV . Cleavage of Disulfide Bond sSection V. Selective Cleavage by Chemical MethodsSection VI . Selective Cleavage with EnzymesSection VII . Separation of PeptidesSection VIII . Sequence Analysi sSection IX. Chemical Modificatio nSection X. Methods of Peptide Synthesis

VOLUME XLVIII

ENZYME STRUCTURE (PART F)

Section I . Molecular Weight Determinations and Related ProceduresSection II . Interactions

VOLUME XLIX

ENZYME STRUCTURE (PART G)

Section I . Conformation and TransitionsSection II . Conformation : Optical SpectroscopySection III. Resonance Techniques

VOLUME L

COMPLEX CARBOHYDRATES (PART C)Section I . Analytical MethodsSection II . PreparationsSection III . Carbohydrate-Binding ProteinsSection W. Biosynthesi sSection V. Degradation

VOLUME LI

PURINE AND PYRIMIDINE NUCLEOTIDE METABOLIS M

BIOSYNTHETIC ENZYME SSection I . Activation of Ribose PhosphateSection II . De Novo Pyrimidine Biosynthesi s

A. Single Enzyme sB. Enzyme Complexe s

Section III. De Novo Purine Biosynthesi sSection W. Deoxynucleotide SynthesisDEGRADATIVE AND SALVAGE ENZYMESSection V. Nucleotidases and NucleosidasesSection VI . Pyrimidine Metabolizing Enzyme s

A. KinasesB. Deaminase sC. Phosphorylases and trans-Deoxyribosylase

Section VII . Purine Metabolizing EnzymesA. KinasesB. DeaminasesC. Phosphorylase sD. Phosphoribosyltransferase sE. General Methods

VOLUME LI I

BIOMEMBRANES (PART C : BIOLOGICAL OXIDATIONS)

Section I . General OverviewSection II . Microsomal Electron Transport and Cytochrome P-45 0

SystemsA. Complex and Resolved SystemsB. ReconstitutionC. General MethodsD. Specific Assay Methods

Section III. Other Hemoprotein SystemsA. Genera lB. Hemoglobin and MyoglobinC. Catalases and Peroxidase s

VOLUME LIII

BIOMEMBRANES (PART D: BIOLOGICAL OXIDATIONS)

Section I . Electron Transfer Complexe sSection II. CytochromesSection III. Nonheme MetalloproteinsSection IV . Flavoprotein sSection V . QuinonesSection VI . Other Microbial Electron Transport Systems

VOLUME LIV

BIOMEMBRANES (PART E : BIOLOGICAL OXIDATIONS)

VOLUME LV

BIOMEMBRANES (PART F : BIOENERGETICS )

Section I. Organelles and MembranesSection II. ATP Synthesis and Regulatio n

A. Phosphorylation Reactions and RegulationB. ATPase ComplexesC. Coupling FactorsD. Specialized Reagent sE. Model Systems

Section III . Membrane Potential and Intramitochondrial p HSection IV . Reconstitution

VOLUME LVI

BIOMEMBRANES (PART G : BIOENERGETICS)

Section I. Biogenesis of MitochondriaSection II . Genetic ApproachesSection III . Membrane Organization

A. Compartmentalizatio nB. Sidedness Reagents

Section IV. TransportA. Methods for Measuring Transpor tB. Isolation of Carriers and Reconstitutio n

Section V . Specialized Technique sA. Functio nB. Structure

VOLUME LVII

BIOLUMINESCENCE AND CHEMILUMINESCENC E

Section I. Firefly LuciferaseSection II . Bacterial LuciferaseSection III . Renilla reniformis LuciferaseSection IV. AequorinSection V . Cypridin aSection VI. Earthworm BioluminescenceSection VII. Pholas dactylusSection VIII . Chemiluminescent Technique sSection IX . Instrumentation and Method s

VOLUME LVIII

CELL CULTURE

Section I . Basic MethodsA. Laboratory Requirements and MediaB. General Cell Culture Techniques

Section II . Specialized TechniquesA. MetabolismB. Genetics, Hybridization, and Transformatio nC. Virus Preparation

Section III . Specific Cell Lines

VOLUME LIX

NUCLEIC ACIDS AND PROTEIN SYNTHESIS (PART G )

Section I . Transfer RNA and Aminoacyl-tRNA Synthetase sSection H. Ribosomes

VOLUME L X

NUCLEIC ACIDS AND PROTEIN SYNTHESIS (PART H)

Section I . Initiation of Protein SynthesisSection II . Peptide Chain Elongation