NEW Kinetex Biphenyl - Phenomenex Kinetex Biphenyl • Remarkable Separation Power...

download NEW Kinetex Biphenyl - Phenomenex Kinetex Biphenyl • Remarkable Separation Power • Rugged and Reliable

of 12

  • date post

    09-Mar-2019
  • Category

    Documents

  • view

    214
  • download

    0

Embed Size (px)

Transcript of NEW Kinetex Biphenyl - Phenomenex Kinetex Biphenyl • Remarkable Separation Power...

Core-Shell Technology

Biphenyl TM

S

TMS

Biphenyl

TMS

TMS

Biphenyl

TMSTMS

Selectivity that a C18 just cant give you!

Kinetex Biphenyl NEW

Remarkable Separation Power

Rugged and Reliable

100 % Aqueous Stable

2

Kinetex Core-Shell AdvantageKinetex Core-Shell Technology produces increased efficiencies over traditional,

fully porous columns on the market, yielding remarkable chromatographic resolu-

tion, higher peak capacities, and greater sensitivity, so labs can get the most out

of every HPLC/UHPLC analysis.

Less Band Broadening

Kinetex Core-Shell Fully Porous

More Band Broadening

2014 Phenomenex, Inc. All rights reserved.

If you are not completely satisfied with Kinetex core-shell columns, send in your comparative data to a similar product with the Kinetex column within 45 days for a FULL REFUND.

3

Core-Shell Technology

Biphenyl

TMSTMS

Biphenyl

TMSTMS

Biphe

nyl

TMS

TMS

Bip

heny

l

TMS

TMSIntroducing

the High Performance Kinetex Biphenyl!

Transform your reversed phase analysis ............................ pp. 4-5Comprehensive pain panel testing ..................................... p. 6Extended lifetime with biological samples .......................... p. 7Wide range of Kinetex selectivities ..................................... p. 8Full scalability from HPLC to UHPLC ................................. p. 9Reproducibility and quality you can rely on ........................ p. 10Ordering information ........................................................... p. 11

Core-Shell Technology

Phenomenex l WEB: www.phenomenex.com

4

Transform Your Reversed Phase AnalysisThink high performance, enhanced retention, and the ability to go where a tra-

ditional C18 cant. The Kinetex Biphenyl offers the high performance benefits

of a core-shell particle with a unique stationary phase capable of becoming

the go-to selectivity for reversed phase method development. Use Kinetex

Biphenyl columns to get enhanced retention, higher sensitivity, and overall

better results; especially for aromatic compounds.

Waters and SunFire are registered trademarks of Waters Corporation. Phenomenex is in no way affiliated with Waters Corporation. Comparative separations may not be representative of all applications.

Conditions for all columns:Column: Kinetex 2.6 m Biphenyl

Waters SunFire 3.5 m C18Dimensions: 50 x 2.1 mm

Mobile Phase: A: 5 mM Ammonium acetate with 0.1% Acetic acid

B: Methanol with 5 mM Ammonium acetate with 0.1% Acetic acid

Gradient: Time (min)0 2 5

5.2 8

8.01 11

%B2 2

80 98 98 2 2

Flow Rate: 0.45 mL/minTemperature: 40 C

Detection: MS/MS (AB SCIEX API 4000)Sample: 1. 15-Acetyldeoxynivalenol

2. DAS 3. FB1 4. HT2 Toxin 5. FB2 6. T2 Toxin 7. Aflatoxin M1 8. Aflatoxin G2 9. Ochratoxin A 10. Aflatoxin G1 11. Aflatoxin B2 12. Aflatoxin B1

Waters SunFire 3.5 m C18

Inte

nsity

, cp

sIn

tens

ity, c

ps

0.0

5.0e5

1.0e6

1.5e6

1.7e6

5 6 7 min

21951

1

7

10

1112

2

435,6

8,9

Ap

p ID

219

51A

pp

ID 2

1952

21952

0.0

5.0e5

1.0e6

1.5e6

1.7e6

5 6 7 min

18,7

1011

12,2

43 6

9,5

Enhanced Retention and Sensitivity

Mycotoxins Kinetex 2.6 m Biphenyl

5

Core-Shell Technology

01 2 3 4 5 6 min

%

100

Enhanced Separation Power

Fused-Core is a registered trademark of Advanced Materials Technology, Inc. Phenomenex is in no way affiliated with Advanced Materials Technology, Inc. Comparative separations may not be representative of all applications.

Fused-Core 2.7 m C18

Inte

nsity

, cp

s

Inte

nsity

, cp

s

Kinetex Biphenyl is a high efficiency core-shell product capable of adding

extra separation power to your analysis of non-polar and polar compounds.

Imagine what you could do with this new chromatography solution.

1 2 3 min

21992

0.0

2.0e4

4.0e4

1 2 3 min

21993

0.0

2.0e4

4.0e4

Ap

p ID

219

92

Ap

p ID

219

93

Conditions for all columns:Column: Kinetex 2.6 m Biphenyl

Fused-Core 2.7 m C18Dimensions: 50 x 2.1 mm

Mobile Phase: A: Water with 0.1 % Formic acidB: Methanol with 0.1 % Formic acid

Gradient: Time (min)0

0.5 2

4.5 4.51 5.5 5.51

7

%B10 10 25 80 85 85 10 10

Flow Rate: 0.6 mL/minTemperature: 40 C

Detection: MS/MS (AB SCIEX API 4000)Sample: 1. Morphine

2. Hydromorphone 3. Norhydrocodone

Opiate Isomers Kinetex 2.6 m Biphenyl

Customer Example - Polar Therapeutic Drug Kinetex 5 m Biphenyl vs. Fully Porous 5 m C18

Share your Kinetex success stories at:

www.phenomenex.com/KinetexShare

Impressive Increase in Resolution

~2x Increase in Retention

BiphenylC18

Phenomenex l WEB: www.phenomenex.com

6

Comprehensive Pain Panel Testing

Samples: 1. Morphine 2. Oxymorphone 3. Hydromorphone 4. Naloxone 5. Codeine 6. 6-MAM 7. Oxycodone 8. Naltrexone 9. Hydrocodone 10. Norfentanyl11. Tramadol12. Normeperidine13. Meperidine14. Fentanyl15. Propoxyphene16. Norpropoxyphene17. EDDP18. Methadone

Samples: 1. Flurazepam 2. Midazolam 3. Lorazepam 4. Clonazepam 5. Oxazepam 6. Hydroxyalprazolam 7. Nordiazepam 8. Flunitrazepam 9. Temazepam10. Alprazolam11. Diazepam

Samples: 1. Amphetamine 2. Methamphetamine 3. MDA 4. MDMA 5. MDEA 6. Benzoylecgonine 7. Meprobamate 8. PCP 9. Carisoprodol10. Sufentanil

Pain Panel Screen Kinetex 2.6 m Biphenyl

Opiates Benzodiazepines Other Drugs of Abuse

Inte

nsity

, cp

sIn

tens

ity, c

ps

Inte

nsity

, cp

s

Inte

nsity

, cp

s

Conditions for all separations:Column: Kinetex 2.6 m Biphenyl

Dimensions: 50 x 3.0 mmPart No.: 00B-4622-Y0

Mobile Phase: A: Water with 0.1 % Formic AcidB: Methanol with 0.1 % Formic Acid

Gradient: Time (min)0

0.5 2

4.5 4.51 5.5

5.51 7

%B10 10 25 80 85 85 10 10

Flow Rate: 0.6 mL/minTemperature: 40 C

Detection: MS/MS (AB SCIEX API 5000)

Important methods demand high performance and complete reliability. If a

system or column interrupts the flow of samples, time and money can be lost.

The new Kinetex Biphenyl ensures that your comprehensive screens display

the needed performance with a ruggedness and reliability that will keep you

at ease.

22108

1 2 3 4 5 6 min0.0

1.0e6

2.0e6

1 2 3 4 5 6 min0

1.0e5

2.0e5

3.0e5

4.0e5

5.0e5

6.0e5

7.0e5

22109

1 2 3 4 5 6 min

22110

0

2.0e5

4.0e5

6.0e5

8.0e5

1.0e6

1.2e6

1.4e6

Ap

p ID

221

08

Ap

p ID

221

09

Ap

p ID

221

10

Full Panel

Find more Clinical applications at:

www.phenomenex.com/ClinicalApps

22104

0 1 2 3 4 5 6 min0

2.0e5

4.0e5

6.0e5

8.0e5

1.0e6

1.2e6

1.4e6

1.6e6

1.8e6

2.0e6

2.2e6

Ap

p ID

221

04

7

Core-Shell Technology

Improved Retention of Clinical SamplesPain Panel - Opiates

Conditions for all columns:Column: Kinetex 2.6 m Biphenyl

Fused-Core 2.7 m C18Dimensions: 50 x 2.1 mm

Mobile Phase: A: Water with 0.1 % Formic acidB: Methanol with 0.1 % Formic acid

Gradient: Time (min)0 4

4.5 4.51

6

%B5 95 95 5 5

Flow Rate: 0.5 mL/minTemperature: 40 C

Detection: MS/MS (AB SCIEX API 4000)Sample: 1. Buprenorphine

2. Carisoprodol 3. Codeine 4. Fentanyl 5. Hydrocodone 6. Hydromorphone 7. Meperidine 8. Meprobamate 9. Methadone 10. Morphine

11. Naloxone 12. Naltrexone 13. Norbuprenorphine 14. Norfentanyl 15. Normeperidine 16. Oxycodone 17. Oxymorphone 18. Tramadol

Inte

nsity

, cp

s

Ret

entio

n Ti

me

(min

)

Kinetex Biphenyl Stability in Urine (First 1000 injections)

Inte

nsity

, cp

s

Fused-Core is a registered trademark of Advanced Materials Technology, Inc. Phenomenex is in no way affiliated with Advanced Materials Technology, Inc. Comparative separations may not be representative of all applications.

Kinetex 2.6 m Biphenyl Fused-Core 2.7 m C18

1 2 3 4 5 min

21972

0.0

2.0e5

4.0e5

6.0e5

1 2 3 4 5 min

21974

0,0

2,0e5

4,0e5

6,0e5

7-Aminoclonazepam7-AminoflunitrazepamAlprazolamAmphetamineBenzoylecgonineBuprenorphineClonazepamCodeineEstazolamFlunitrazepamHydrocodoneHydromorphoneLorazepamMDMAMethadoneMidazolamMorphine