Mult iNA

ShimadzuMicrochip Electrophoresis System for DNA/RNA Analysis

C297-E062B

M i c r o c h i p E l e c t r o p h o r e s i s S y s t e m f o r D N A / R N A A n a l y s i s

M u l t i N A

Bringing together Shimadzu microchip technology and automatic analysis technology provides

an alternative to agarose gel electrophoresis that is cheaper, faster, and more sensitive than

ever before. Combined with even greater analytical precision, this easy-to-use equipment lifts

electrophoresis systems to a new level.

This next-generation microchip electrophoresis system will bring about revolutionary changes

in life science laboratories.

P 4 - Features

P 6 - Analysis Procedures

P 7 - MultiNA Control & Viewer Software

P 8 - Applications

P 10 - MultiNA Microchip & MultiNA Reagent KitContents

Shimadzu Microchip Electrophoresis System for DNA/RNA Analysis

MultiNA

4

Features

Why is a Laboratory Revolution on the Way?Are you frustrated with agarose gel electrophoresis?

Smiling and adjacent ladder patterns indicate only approximate size

Agarose provides lower sensitivity and it is hazardous with ethidium bromide staining

Data management is tedious

Numerous manual operations take up too much time and effort

It takes too long to get any data

Precast gels are expensive

5

With Shimadzu’s renowned microchip technology, a new electrophoresis analysis platform provides an alternative to address your problems with agarose gel electrophoresis.

Yes, MultiNA!!

Feature 1Lower Analysis CostsA sophisticated reusable microchip achieves lower running costs for consumables per analysis than agarose gel electrophoresis. (*1) Based on list price in Japan markets, Shimadzu calculations indicate costs of 25 yen ( US 0.20*) per DNA analysis and 35 yen per RNA analysis ( US 0.30*). (*1): Shimadzu survey comparison results of list prices of commercial products.

Feature 2Greater SpeedHigh-speed automatic operation for up to 120 analyses (*2)

75 seconds/analysis cycle time (*3)

Simply set up the samples and the separation buffer for automated analysis of up to 120 samples. Up to four microchips can be placed for parallel processing of analysis pretreatment and electrophoresis, resulting in a cycle time of just 75 seconds per analysis. (*2) An analysis schedule can include up to 120 samples while the instrument can accept a maximum of 108 samples (96 + 12 extra). (*3) For DNA standard analysis (DNA-1000 kit/premixed) using four microchips. The 75 seconds/analysis cycle time does not include time required for carrying out the first wash, final wash and initial analysis.

Feature 3High Sensitivity DetectionThis instrument is equipped with a LED-excited fluorescence detector. It�s approximately 10 times more sensitive than ethidium bromide staining (based on Shimadzu’s own study)

Feature 5Outstanding Ease of UseThe control and viewer software provides a refined GUI that makes analysis operation extremely simple. First make an analysis schedule, and then simply load the reagents and samples into the instrument and click the [Start] button on the screen to start the analysis. A wide variety of useful functions enables users to perform analysis schedule design and data processing more quickly and simply.

Feature 4High Resolution and High ReproducibilitySelect the proper separation buffer that suits the sample. Internal lower and upper markers in the reagent kit ensures high analysis reliability and reproducibility.

SS

*1 US = 120 Japanese YenS

6

Analysis Procedures

Analysis Procedures

1Run the MultiNA control software on the PC and simply click the appropriate wells. You can then register the sample sheet in the analysis schedule.

3Place the microchips and the prepared samples and reagents on their prescribed positions in the instrument.

4 Click the [Start] button on the PC.

2 Prepare the samples and reagents.

Leave the rest up to the instrument. All operations are handled automatically,

including separation buffer filling, sample applying, electrophoresis detection,

microchip washing, and data analysis.

7

MultiNA Control & Viewer Software

MultiNA Control & Viewer SoftwareSize markers automatically correct electrophoresis results for automatic size prediction and quantitation. Moreover, users can perform either automatic or manual reanalysis of their data. Even if analysis of all samples is not yet finished, analysis results of completed samples can be confirmed. Confirm current operating status with a quick glance at the software�s well status display, analysis schedule list, and chip status display. The separation electropherogram is displayed in real time of the analysis. Extensive operation and maintenance functions are provided, including analysis performance check functions.User-friendly data processing functions handle importing sample sheets and exporting analysis data in CSV format.

Start analysis button Analysis schedule list

Sample status display

Well status display

Reagent information

Real-time electropherogram display

Display data file button

Gel image

Chip status display

Sample well display

Electropherogram

8

Applications

DNA ApplicationSingle or multiplex PCR products, restriction digests, synthetic oligonucleotides such as primers, and plasmid insert routine checks (size analysis and quantitative analysis)

Sample Report

DNA-500 Ladder AnalysisBlue: PCR amplification product obtained using Shimadzu’s Norovirus Detection Kit

G1(86bp) G2(98bp)

Red: 25 bp DNA ladder

DNA-1000 Ladder Analysis

DNA-2500 Ladder Analysis

Color: phiX174 Hinc II digest (TaKaRa) Final concentration: 10 ng/�lRed: phiX174-Haelll digest (Promega) Final concentration: 10 ng/�l

pGEM DNA markers (Promega)Final concentration: 10 ng/�l

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9

RNA ApplicationExpression analysis, transcriptome analysis, quality check of in vitro synthesized mRNA in a cell-free protein synthesis system, and purity checks on extracted RNA (ribosomal RNA subunit ratio and mRNA analysis)

Sample Report

Example Using RNA KitRNA6000 ladder (Ambion)Final concentration: 25 ng/��l

Example Using RNA KitRat heart total RNA (Ambion)Final concentration: 25 ng/��l

10

MultiNA Microchip & MultiNA Reagent Kit

MultiNA MicrochipA special coating, which is applied to the high-purity quartz chip created using Shimadzu�s unique photolithography technologies, allows an enormous number of reuses and ensures high-quality analysis.

MultiNA Reagent Kit

Part No.292-27900-91

Part NameMicrochip, Type DR-C

Reagent Kit TypeDNA-500 kit (25 bp to 500 bp)DNA-1000 kit (100 bp to 1,000 bp)DNA-2500 kit (100 bp to 2,500 bp)RNA kit (up to 28S rRNA)

Kit Contents(1) Separation buffer(2) Marker (low molecular, high molecular)*Kit does not include fluorescent dye (SYBR¤ Gold (DNA Analysis) and SYBR¤ Green2 (RNA Analysis) are recommended).

Part No.292-27910-91

Part NameDNA-500 kit (1,000 analyses)

292-27911-91 DNA-1000 kit (1,000 analyses)292-27912-91 DNA-2500 kit (1,000 analyses)292-27913-91 RNA kit (1,000 analyses)

11

SpecificationsInstrument

Item

Sample rack

Pretreatment

Electrophoresis Voltage

Analysis Cycle time

Detection Method

Separation Size Range

Maximum Salt Concentration

Min. Detection Limit

Resolution

Quantitation Range

Quantitation Accuracy

QuantitationRepeatability

Microchip

Specification

External DimensionsWeightPower Supply

Sizing Accuracy

96 wells (12 x 8), minimum application volume required: 5�L (on-chip mixing, with aluminum seal), 6�L (premixed, with aluminum seal)

Automatic sample injection, automatic separation buffer replenishing, automatic chip cleaning

Max. rated voltage: 1.5kV, max. current: 250�A

75s (using four chips) * DNA standard analysis (DNA-1000/premixed). This does not include time required for carrying out the first and final wash and initial analysis.

DNA analysis: 0.5ng/�L to 50ng/�L (at 10mM Tris-HCI, containing 50mM KCl and1.5mM MgCl2)RNA analysis: 25ng/�L to 500ng/�L (total RNA), 25ng/�L to 250ng/�L (mRNA) (10mM Tris-HCI buffer, containing 1mM EDTA)

DNA analysis: 0.2ng/�L (at 10mM Tris-HCI buffer, containing 50mM KCl and 1.5mM MgCl2)RNA analysis: 5ng/�L (total RNA), 25 ng/�L (mRNA) (at 10mM Tris-HCI buffer, containing 1mM EDTA)

DNA analysis: –30% (at 10mM Tris-HCI buffer, containing 50mM KCL)

W 415 mm x D 545 mm x H 508 mm43 kg100 V to 120 V, 220 V to 240 (CE Marking) 300 VA max.

RNA analysis: CV10% or less(CV20% or less for eukaryotic-origin total RNA at concentrations of 150ng/�L or more)

LED-excited fluorescence detector (470nm excitation wavelength) *Class 1 LED product

25bp to 500bp (DNA-500Kit)100bp to 1000bp (DNA-1000Kit)100bp to 2500bp (DNA-2500Kit)Up to 28S rRNA (5.0 knt) (RNAKit)

5bp (25bp to100bp), 5% (100bp to 500bp), 10% (500bp to 1000bp), 20% (1000bp to 2500bp)–5bp (25bp to 100bp), –5% (100bp to 500bp), –15% (DNA-1000, DNA-2500)

DNA analysis: 10mM Tris-HCI containing125mM KCl or NaCl max.RNA analysis: 10mM Tris-HCI, containing1mM EDTA max.

Quartz, 23mm separation channel length, on-chip electrodes (insert up to four microchips)

* Note) Control PC is not supplied with the instrument. Purchase the control PC separately.

SYBR¤ is a registered trademark of Invitrogen Corporation.Microsoft¤ and Windows¤ are registered trademarks of Microsoft Corporation.Pentium¤ is a registered trademark of Intel Corporation and its subsidiaries.

* Note) The analysis performance specifications above are based on Shimadzu standard analysis conditions and standard samples.* Note) Reagent kits and microchips are not included as part of the MultiNA instrument�s standard accessories.

Controller and Viewer SoftwareItem

Controller

Reports

Minimum requirements for PC

Data Processing

SpecificationCreating analysis schedules, realtime control, automatic analysis pretreatment, automatic analysis post-treatment, automatic error processing, analysis log management, analysis performance checks

Batch display/detailed display of gel images/pherograms, automatic quantitation and size prediction by size markers, data searching, data import/export, manual editing and re-analysis

Multilevel data display, tree display of samples/files, RNA structural comparison, analysis performance check results, analysis log

Intel¤ Pentium¤ 3 or compatible processor, 1GHz minimum (Requires 512 MB memory minimum, 40 GB hard disk minimum, and display resolution of 1024 x 768 pixels minimum)OS: Windows¤ XP SP2Ethernet (100BASE-TX, single-port minimum)*Expansion LAN port and LAN cable are not supplied with the instrument. Users should purchase these separately.

Printed in Japan 3295-04815-30A-IK

The contents of this brochure are subject to change without notice.

SHIMADZU CORPORATION. International Marketing Division3. Kanda-Nishikicho 1-chome, Chiyoda-ku, Tokyo 101-8448, Japan Phone: 81(3)3219-5641 Fax. 81(3)3219-5710URL http://www.shimadzu.com

JQA-0376

Founded in 1875, Shimadzu Corporation, a leader in the development of advanced technologies, has a distinguished history of innovation built on the foundation of contributing to society through science and technology. We maintain a global network of sales, service, technical support and applications centers on six continents, and have established long-term relationships with a host of highly trained distributors located in over 100 countries. For information about Shimadzu, and to contact your local office, please visit our Web site at www.shimadzu.com

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