Microfluidic Paper-based Biomolecule Preconcentrator Based ... · Microfluidic Paper-based...
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SUPPLEMENTARY INFORMATION: Microfluidic Paper-based Biomolecule Preconcentrator Based on Ion Concentration Polarization Sung Il Han a* , Kyo Seon Hwang b* , Rhokyun Kwak b** and Jeong Hoon Lee a** 1. Nafion patterning on various adhesive tapes Figure S1. Patterned Nafion on various adhesive tapes. (a) 3M cellophane tape, (b) 3M double-sided tape, (c) kapton tape, and (d) 3M magic tape. 2. Non-specific binding of preconcentrated samples in paper Figure S2. In the same experimental setup used in Fig. 4b, we confirmed the preconcentrated fluorescent dyes are still free from non-specific binding to the paper. As can be seen, when the applied voltage is turned off at 300 sec, all preconcentrated dyes are washed out by lateral fluid flow. Supporting Video 2 is also available for preconcentration action (t <300 sec) and washing action (t >300 sec). ! t < 300 sec : V = 50 V ! t > 300 sec : V = off 1 mm 0 s 60 sec 120 sec 300s 200s 100s 330s 360s 420s patterned Nafion lateral fluid flow Electronic Supplementary Material (ESI) for Lab on a Chip. This journal is © The Royal Society of Chemistry 2016
Transcript of Microfluidic Paper-based Biomolecule Preconcentrator Based ... · Microfluidic Paper-based...
SUPPLEMENTARY INFORMATION:
Microfluidic Paper-based Biomolecule Preconcentrator Based on Ion Concentration Polarization
Sung Il Hana*, Kyo Seon Hwangb*, Rhokyun Kwakb** and Jeong Hoon Leea**
1. Nafion patterning on various adhesive tapes
Figure S1. Patterned Nafion on various adhesive tapes. (a) 3M cellophane tape, (b) 3M double-sided tape, (c) kapton tape, and (d) 3M magic tape. 2. Non-specific binding of preconcentrated samples in paper
Figure S2. In the same experimental setup used in Fig. 4b, we confirmed the preconcentrated fluorescent dyes are still free from non-specific binding to the paper. As can be seen, when the applied voltage is turned off at 300 sec, all preconcentrated dyes are washed out by lateral fluid flow. Supporting Video 2 is also available for preconcentration action (t <300 sec) and washing action (t >300 sec).
! t < 300 sec : V = 50 V!! t > 300 sec : V = off ! 1 mm!
0 s! 60 sec! 120 sec! 300s!200s!100s! 330s! 360s! 420s!
patterned Nafion!
late
ral fl
uid
flow!
Electronic Supplementary Material (ESI) for Lab on a Chip.This journal is © The Royal Society of Chemistry 2016
