SUPPLEMENTARY INFORMATION:

Microfluidic Paper-based Biomolecule Preconcentrator Based on Ion Concentration Polarization

Sung Il Hana*, Kyo Seon Hwangb*, Rhokyun Kwakb** and Jeong Hoon Leea**

1. Nafion patterning on various adhesive tapes

Figure S1. Patterned Nafion on various adhesive tapes. (a) 3M cellophane tape, (b) 3M double-sided tape, (c) kapton tape, and (d) 3M magic tape. 2. Non-specific binding of preconcentrated samples in paper

Figure S2. In the same experimental setup used in Fig. 4b, we confirmed the preconcentrated fluorescent dyes are still free from non-specific binding to the paper. As can be seen, when the applied voltage is turned off at 300 sec, all preconcentrated dyes are washed out by lateral fluid flow. Supporting Video 2 is also available for preconcentration action (t <300 sec) and washing action (t >300 sec).

! t < 300 sec : V = 50 V!! t > 300 sec : V = off ! 1 mm!

0 s! 60 sec! 120 sec! 300s!200s!100s! 330s! 360s! 420s!

patterned Nafion!

late

ral fl

uid

flow!

Electronic Supplementary Material (ESI) for Lab on a Chip.This journal is © The Royal Society of Chemistry 2016