January 2006, Volume - 1 Post-it - Genetix...

1

January 2006, Volume - 1


Genetix Biotech Product Update

REPLI-g KitFor High fidelity whole genome application from small or

precious samples, based on Multiple Displacement

Amplification (MDA) technology,

Benefitsl Reproducible whole genome amplification from a

variety of starting materials — including genomic DNA,

fresh or dried blood, buffy coat, and morel Highly uniform amplification — across the whole

genome with minimal amplification biasl Only one sample collection required — obtain unlimited

DNA for all your downstream applicationsl Uniform DNA yield — enabling direct use in downstream

applications without quantification

Now Whole Genome Amplification (WGA) is not a Difficult Task!

Eliminating Sample Limitations

Applications: REPLI-g amplified genomic can be used in a

variety of downstream applications, including:l SNP and STR genotyping analysis RFLP analysisl Comparative genome hybridization

WGA - Single Tube

Protocol

AlkalineDenature/Lysis

Neutralize

Repli-g Mix

Incubate at 30°C

>40 µg DNA

Post-itQiagen .................................... 1-4

Biometra ..................................... 1

MBI,Qiagen ................................ 4

Microflex, Amresco ...................... 4

Ambion, MBI ............................... 5

Spectrum, Cayman ...................... 6

Ambion, Amresco ........................ 6

Tosoh-Bioscience, Virogen ............ 6

Kimble Pharma ............................ 6

Vivascience ................................. 7

AB-Biodisk .................................. 8

Nunc .......................................... 9

Nunc, Nalgene, Peprotech ......... 10

Nalgene ............................... 11-12

mRNA Purification and cDNASynthesis in the same wellTurboCapture mRNA Kit – For rapid and easy mRNA purification

from cultured cells

l Speed — few protocol steps and minimal hands-on timel Simplicity — mRNA purification and downstream cDNA synthesis and PCR in

the same platel Versatility — mRNA purification also available in 8- and 96-well formatsl Automatable — kit is compatible with robotic workstations

TurboCapture mRNA Kits provide a fast and simple procedure for purifying mRNA.

Large numbers of samples can be processed simultaneously, making the kits

ideal for high-throughput applications such as compound screening and target

validation (e.g., by siRNA screening).

TurboCapture mRNA Kits are available in three formats:l TurboCapture 8 mRNA Kit — for mRNA purification in 8-well stripsl TurboCapture 96 mRNA Kit — for mRNA purification in 96-well platesl TurboCapture 384 mRNA Kit — for mRNA purification in 384-well plates

Fast and Easy Solutions for

Protein synthesis &

FractionationEasyXpressTM Protein Synthesis Kits – Fast and Efficient invitro synthesis of recombinant proteins

l Fast procedure - go from gene to protein in a single dayl High yields - up to 600 ug protein per ml reaction volume in just one hourl A scalable, convenient procedure - pre-aliquoted extracts, color-coded

reagents, and easy-to-follow protocolsl Significant time and cost-savings

- no cloning, transformation,

fermentation, or specialized

equipment required

The EasyXpress product range

has now been expanded with the

launch of several new kits:l EasyXpress biotin labeling kit-

guaranteed efficient detection or

site directed biotinylation for

interaction studiesl EasyXpress large-scale protein

synthesis kits-upto 5 mg purified

protein in just 4 hours.l EasyXpress protein Synthesis

Insect Kit-fully active and

correctly folded mammalian

proteins with posttranslational

modfifications.

& Improved

l Motorized heated lidl Unique: Motorized plate lifterl Small footprint, external controllerl Designed specifically for robotic systemsl Integrated cycler with external controllerl Proven Peltier technologyl Lid opening angle 1100

l Adjustable lid pressurel Small footprintl Available with 96 well (silver) or 384 well

(aluminum) blockl Low power consumptionl Communication with PC via serial RS232

protocoll Instrument provides extensive status report

T-Robot Thermocycler –Specially designed for integration in roboticenvironments

l With T-GRADIENT 12 different annealing

temperatures can be tested in single run.

l Temperature gradient of up to 40ºC

l Silver block for fast heating and cooling

l Easy to use software featuring gradient

l Software Touchdown. Time increment. Pause. Alarm. Online help. Temp.

Increment. Gradient calculator.

l Program memory Total capacity: 1500 steps 10 directories with 100 pgms

each, maximum 99 steps per program, maximum 99 cycles per loop

lBlock 96 well: silver, 48 well: silver, 384 well: Aluminum, in situ: Aluminum

lCapacity TGradient 96: 96 x 0.2ml tubes; 96 well Microplates; Strips

TGradient 48: 48 tubes 0.5ml

T-Gradient ThermocyclerFind the perfect annealing temperature in a single run

Test your new primer pairs with unknown

annealing temperature quickly and

optimize new protocols in short time

Three independent Thermocyclers in one

housing

Three different block formates available: 20

well 48 & combi welll T3000 based on latest Peltier Technology to

achieve excellent heating and cooling rates.l Better programming due to completely

revised software.l Bigger memory with program storage in

individual subdirectories.l Runs three programs at same time

Three heated lids with

automatic pressure

control (smart Lid

Technology)

Context sensitive soft

keys.

Program stored in

individual subdirectories

Small, Strong and hot!

New

Please askfor new

catalogs

NEW

NEW

NEW

RE-Newsletter-Volume1FF-October.pmd 1/7/2006, 2:49 PM1

2


A general-use kit for reproducible fractionation and enrichment., Separate proteins according to their cellular location., Efficiently isolate and subfractionate active nucleic acid binding proteins., Fast, efficient albumin and igG depletion of serum and plasma samples., Separation of phosphorylated and unphosphorylated proteins., Highly Specific isolation of Glycoproteins

Qproteome kits - making the proteome more manageableA major problem facing protein researchers is the sheer complexity of the proteome. Purification and analysisof low-abundance species is especially problematic. QIAGEN has used its expertise in sample preparation todevelop the Qproteome range of kits, for standardized and reproducible protein fraction and depletion.

Qproteome Kit Offers:• Reduced sample complexity according to highly specific separation criteria — efficient

isolation of targeted subsets of proteins for easier analysis of low-abundance species• Simple, easy-to-use kit formats — no specialized equipment required• Highly reproducible, standardized separation, reliable results, time after time• Intact, native-conformation proteins — suitable for enzymatic assays and other activity-

based downstream applications

Cat No. Qproteome kit Protein categories Sample size Fractions isolated Price/Rs.

37900 Qproteome Bacterial Protein Prep Kit Soluble bacterial proteins 25 ml bacterial culture/ml lysis buffer Soluble bacterial proteins37901 Qproteome Mammalian Protein Prep Kit Total mammalian proteins 5–10 x 106 cells per ml lysis buffer Total mammalian proteins37512 Qproteome Soluble Protein Separation Kit Whole proteome 106–107 cells or 500 µl protein sample 0.5–4 mg protein in 6 fractions 16,834/-

(total protein 0.5–4 mg) separated according to protein solubility37502 Qproteome Cell Compartment Kit Organelle-/cell compartment-specific 5 x 106 cells 0.5–1 mg protein divided between 16,834/-

cytosolic, membrane, nuclear, & cytoskeletal protein fractions

37582 Qproteome Nuclear Subfractionation Kit Nuclear and nucleic-acid binding proteins 5 x 106 – 1 x 107 cells 1.5-2.5 mg total protein divided 20,287/-between cytosolic proteins,“insoluble” nuclear proteins (e.g.,histones), and 3 nucleic-acid–binding protein subfractions (50-100 µg each)

37541 Qproteome Total Glycoprotein Kit Glycosylated proteins 107 cells or 50 µl serum Separate enriched fractions 25,466/-37551 Qproteome Mannose containing 20-150 µg 25,466/-

Glycoprotein Kit glycoproteins carrying lectin-37561 Qproteome Sialic Glycoprotein Kit specific glycan moieties 25,466/-37571 Qproteome O-Glycan 25,466/-

Glycoprotein Kit

37521 Qproteome Albumin/IgG Depletion Kit Serum and plasma proteins 25 µl serum or plasma 0.5–0.8 mg albumin and IgG-free 18,560/-serum or plasma protein sample

37101 Phosphoprotein Purification Kit Phosphorylated proteins 1 x 107 cells Unphosphorylated proteins and 22,733/-phosphorylated proteins (typically10% total protein)

Efficient Fractionation of a Cell Lysate

Fractionation of an NIH-3T3 cell lysate sample

using the Soluble Protein Separation Kit. CL:

cleared lysate;M: markers.

kDa M CL 1 2 3 4 5 6

200 –

97 –

66 –

55 –

36 –

31 –

21 –

14 –

6 –

Fraction Number

Cell pellet (approx.5 x 106 cells)

Add ExtractionBuffer CE1


Add Benzonase &Extraction Buffer CE3


Fraction 4Cytoskeletal proteins

Fraction 1CytosolicProteins



Cell Compartment KitFractionation Procedure

Catalog No. Name Format Size282-2 Anaplasma Antibody Test Kit, cELISA cELISA, stripwell 2 plates282-5 Anaplasma Antibody Test Kit, cELISA cELISA, stripwell 5 plates273-2 Babesia caballi Antibody Test Kit, cELISA cELISA, stripwell 2 plates274-2 Babesia equi Antibody Test Kit, cELISA cELISA, stripwell 2 plates288-100 Bluetongue Virus Antibody Test Kit AGID 100 tests287-2 Bluetongue Virus Antibody Test Kit, cELISA cELISA, stripwell 2 plates287-5 Bluetongue Virus Antibody Test Kit, cELISA cELISA 5 plates284 Bovine Leukemia Virus Antibody Test Kit ELISA, stripwell 1 plate284-5 Bovine Leukemia Virus Antibody Test Kit ELISA, stripwell 5 plates

298 Bovine Spongiform Encephalopathy Antigen Test Kit, Immunohistochemistry Immunohistochemistry 50 tests275-5 Brucella Antibody Test Kit, ELISA ELISA, stripwell 5 plates289-2 Caprine Arthritis-Encephalitis Virus Antibody Test Kit, cELISA cELISA, stripwell 2 plates289-5 Caprine Arthritis-Encephalitis Virus Antibody Test Kit, cELISA cELISA, stripwell 5 plates400-200 Equine Infectious Anemia Virus Antibody Test Kit AGID 200 tests299-10 Escherichia coli Antigen Test Kit (K99 Pilitest) Latex agglutination 10 tests299-25 Escherichia coli Antigen Test Kit (K99 Pilitest) Latex agglutination 25 tests294 Malignant Catarrhal Fever Virus Antibody Test Kit cELISA, stripwell 1 plate280-2 Neospora caninum Antibody Test Kit, cELISA cELISA, stripwell 2 plates280-5 Neospora caninum Antibody Test Kit, cELISA cELISA, stripwell 5 platesTSE SET Prion Protein Detection Kit, IHC Immunohistochemistry 50 te

Veterinary Diagnostic

Test Kits & Reagents

FastLane Cell cDNA Kit It allows highly sensitive and reproducible results in real-time, two-step RT-

PCR as well as accurate detection over a wide linear range from 1 x 105 cells

down to one cell.l No RNA purification - from cells to cDNA in only 4 steps and less than

45 minutesl Short and simple workflow - easy parallel processing of several samplesl High sensitivity - detection of even low-abundance transcriptsl Detection of RNA only - accurate gene expression analysis with less

effort

l Reproducible Analysis of a Single cDNA Sample

No RNA purification For high-speed preparation of cDNA for use in real-time RT-PCR.

cDNA prepared from

HeLa ACC cells using

the FastLane Cell cDNA

Kit was analyzed 16-

times on the ABI PRISM

7700 using the

QuantiTect Probe PCR

Kit and the QuantiTect

Gene

Cancer genes were analyzed by real-time, two-step RT-PCR with SYBR Green detection

using QuantiTect Primer Assays. The cell lysis and RT steps were performed using either the

FastLane Cell cDNA Kit or a similar kit from Supplier AIV.

FastLane Cell cDNA Procedure

Applications

l Validation of siRNA-mediated gene knockdown

l Selection of potential drugs

l Detection of stimulated gene regulation

l Other gene expression analyses

NEW

NEW


3


Suitable for collecting and

dispensing distilled water,

reagents, acids.

Integrated Solutions – Automated Sample Preparation

Models of efficiency – the affordable BioSprint 15 and BioSprint 96BioSprint workstations and kits give you:l A convenient automated procedure-saving

time and effortl A cost-efficient solution-economically priced

workstations for up to 15 or 96 samples per

runl Fast startup and immediate results-with easy

to use, preinstalled BioSprint protocolsl Flexibility and versatility-use the protocols

supplied or easily design your ownl Rapid purification of high quality genomic DNA-

ready to use in sensitive downstream

applications

Efficient Processing of Magnetic Particles

The BioSprint SystemBiosprint workstation and kits provide all you need

for rapid and cost-efficient automated sample

preparation-whatever your throughput needs. The

BioSprint System uses proven MagAttract

magnetic particle technology for purification of

high quality genome DNA for genotyping

applications.

Flurometric 384 well

HDAC Assay kit

Versatilel Analyze all HDACs from

yeast to humanQuantitative: MeasureHDAC activity froml Cell lysatel Purified proteinsl IP proteinsRapid: Process multiplesamplesl Simultaneouslyl Quicklyl ConvenientlyKit Componentsl 384 well black platel HDAC assay bufferl Activator solutionl HDAC assay standardl Trichostatin Al HeLa Nuclear extract

New

Well 1 Well 2

List Price

Rs. 2553/-

Carboys with Spigot,

low-density polyethylene:polypropylene spigot andscrew closure

Genetix Offers

The BioRobot EZ1 workstation purifies high-quality

nucleic acids from clinically relevant samples, such

as whole blood. 1-6 samples are processed in 15-20

minutes. Nucleic acids in sample lysates are isolated

in one step through their binding to the silica surface

of magnetic particles.

Reagents are supplied in pre-filled EZ1 Reagent

Cartridges, which ensures speed and convenience in

loading the BioRobot EZ1. Protocols for nucleic acid

purification are supplied on pre-programmed EZ1

Cards. These protocols provide both on-screen

instructions for the operator and operating commands

for the workstation.

BioRobot EZ1 Workstation delivers:l Rapid purification of high-quality nucleic acids from

a wide range of sample typesl Easy to use workstation requires minimal user

interactionl Affordable, slimline workstationl Flexibility and versatility to suit different types of

lab

BioRobot EZ1Workstation

l Safer, faster operation-process 6 samples in 15-20

minutes using pre-filled Reagentl Simple, walkway automation- just load the workable

and let it go!l Cartridges and pre-programmed EZ1 cards

Programming the workstation is as easy as using a

credit card

1. Insert the card containing the desired protocol into

the IC card slot and start the workstation

2. Add your samples (1-6) and prefilled and sealed

reagent Cartridge to the workstation. Samples are

processed automatically.

3. Remove from the workstation highly pure NA at a

concentration suited to your downstream application

in 1.5 ml microtubes.

A Inserting an EZ1 Card into the BioRobot EZ1. B Loading

sample tubes, elution tubes, filter-tips, and EZ1 Reagent

Cartridges.

EZ1 Kits related to Biorobot EZ1 Workstation

l EZ1 DNA Blood Kits

l EZ1 DNA Tissue Kit

l EZ1 RNA Cell Mini Kit

l EZ1 RNA Tissue Mini Kits

l EZ1 Virus Mini Kit

3steps

For

Rs. 1277/-

Nunc is with you all the way...Great Choice - Great Flexibility

Choice of Surface matters

PolySorp, with high affinity to molecules of a hydrophobic natureMediSorp, has a surface chemistry intermediate between PolySorp and MediSorp. Provides low background signalwith sample containing serum.MaxiSorp with high affinity to molecules with mixed hydrophilic/hydrophobic domains.

MultiSorp, with high affinity to hydrophilic molecules

l Choice of F,C and U bottomsl Raised rims on the wellsl Certified binding homogenicityl Alphanumeric orientation systeml When centrifuging Microwell plates use spacer plate as support.l Fit standard equipment

Plates intended for quantitative and qualitative solidphase immunoassay (eg. ELISA) and binding assay

Walkaway purification of

Nucleic acids has never

been so easy!


4


Remember Santa CruzRemember Santa CruzRemember Santa CruzRemember Santa CruzRemember Santa Cruz When You DemandQuality AntibodiesQuality AntibodiesQuality AntibodiesQuality AntibodiesQuality Antibodies

l Phospho-Specific antibodiesl Intracellular FCM antibodiesl Multicolor FCM systeml Viral Proteinl Bacterial Proteinl Staining Systeml Isotype –Specific secondary antibodiesl F(ab’)2 Secondary antibodiesl Mounting Medial Whole Cell lysatel Acetylation Specific antibodies

And many more...Characterized for WB, IP, FCM & ELISA

RESTRICTION ENDONUCLEASES, NICKING & HOMING ENZYMES

New REases Catalog# Units Prototype Specificity 5'=>3'

LguI ER1931 100

ER1932 500

CseI ER1901 100

ER1902 500

EcoRII ER1921 200

ER1922 1000

HpyF3I ER1881 500

ER1882 2500

PscI ER1871 200

ER1872 1000

SgsI ER1891 300

ER1892 1500

TstI ER1911 100 TstI ^(8/13)CAC(N)6TCC(12/7)^

SapI GCTCTTC(1/4)^

HgaI GACGC(5/10)^

EcoRII ^CC(A/T)GG

DdeI C^TNAG

BspLU11I A^CATGT

AscI GG^CGCGCC

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Supreno is a comfortable nitrileexamination glove formulated forsuperior strength and durability.Textured fingers offer better gripping inwet conditions. Interior polymer coatingmakes Supreno easy to don. Onlinechlorination processing means moreconsistent quality in every box, everytime.Prodduct Code:025Sizes & Quantity:SU-INTXS - XL; 100/BOX l 1000/CASE

Made from a soft polychloropreneformulation, NeoPro offers the barrierprotection of a synthetic with a fit andfeel like natural rubber latex. Morepuncture resistant than latex. Resists abroad range of chemical and biologicalhazards.Prodduct Code:Sizes & Quantity:NP-INTXS - XL; 100/BOX l 1000/CASE

Diamond Grip PlusTM is made from soft,premium natural rubber latex for acomfortable fit. Fully textured forenhanced sensitivity and control.Manufactured with integrated polymertechnologies for powder-free donningand product consistency.Prodduct Code:Sizes & Quantity:DGP-INTXS - XL; 100/BOX l 1000/CASE

Micro One is a highly versatileexamination glove that performs wellin demanding conditions. Made fromsoft latex, Micro One has a natural fitfor reduced hand fatigue. Lightlypowdered to go on easily. Smoothsurface allows for uncompromisedsensitivity.Prodduct Code:Sizes & Quantity:MO-INTXS - XL; 100/BOX l 1000/CASE

CE4 12” Hand Specific Gloves areWashed in microprocessor controlled,two-in-one, washer/dryer launderingsystem to provide very low surfaceparticulates, ionic extractables and non-volatile residues. These gloves areMulti-Class cleanroom compatibility.

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ISO 13485 certifiedEN 374 certifiedEN 455 certified

AMRESCO’s HTS NEXT GEL™ Kit

Save Time - Analyze up to

200 protein samples in 2 hours

Combination of a unique blend of

agarose & NEXT GEL™ Running

Buffer and can be used to analyze

SDS-denatured proteins using

standard horizontal gel apparatus

l Easy To Use - As simple as a

standard agarose, horizontal gell Non-Toxic & Biodegradable – A

unique blend of agarose

combined with the NEXT GEL™

Running Buffer, the HTS NEXT

GEL™ Kit contains no acrylamidel High throughput analysis - Up to

200 samples per gell Contains all reagents required to

run up to 5 gels (up to 1000

samples)l Cost-effective & Easy to use

protein analysisl Separates large proteins (0.2-10

million Daltons) depending on

the concentration of agarose

used (2% agarose yeilds 50 kD -

10 million kD; 4% agarose yeilds

14 kD - 212 kD)

Applications:

Protein expression library screening

Drug screening

Quality control stability studies

Cell and tissue extracts and lysates

Cell supernatants (activated vs. non-

activated)

Fractions from proteins

chromatography by HPLC or FPLC

MAIN PHOTO - HTS NEXT

GELTM (4%)- Stained withCoomassie R-250 Lane 1: MWStandards (wide) (Code: K494)(212 kDa-14.4 kDa). Remaininglanes represent random samplesof the following proteins: BSA (65kDa); Lactate Dehydrogenase (35kDa); Lysozyme (14.4 kDa); LiverHomogenate (14-100 kDa).

INSERT PHOTO - HTS NEXT

GELTM (4%)-Stained withCoomassie R-250 Proteinsamples used: MW Standard(Wide) (Code: K494) (212 kDa-14.4 kDa)’ MW Standards (M/L)(Code: J450) (97 kDa-14.4 kDa)’BSA (65kDa)’ CarbonicAnhydrase (35 kDa).

ZiP™ Reversible Protein Detection KitDestain in less than 5 minutes – Results in 15 minutes!

AMRESCO’s ZiP™ Reversible Protein Detection Kit allows for visualization

of proteins in a polyacrylamide gel that is fast and completely reversible.

5µl of AMRESCO wide rangeprotein marker (Code: K494)was run in each well of a 10%NEXT GELTM and stained usingthe ZIPTM Reversible ProteinDetection Kit. The gel imagewas documented by scanningon a flatbed scanner with a darkbackground placed over top.

l Allows for the obtaining of sequence

data from proteins not detected on

transfer membranesl Offers an established method of

detection when structural analysis is

requiredl Provides a completely reversible

detection method compatible with

most downstream applicationsl Easy to use protocol

Application:

Protein detection

Sequencing

Mass spectrometry

Western blotting

2X PCR MASTER MIXFast and efficient PCR: simply add DNA template and primers

Fermentas 2X PCR Master Mix is a premixed 2X concentrated solution of

Taq DNA polymerase, PCR buffer, MgCl2 and dNTPs. The mixture is optimized

for consistent and efficient routine PCR amplifications.

Features:l Convenient: single tube, single pipetting step.l Ready-to-use: simply add DNA template and primers.l Scaleable: sufficient for 100 standard PCR reactions of 50µl volume: can

be scaled down or up as required.l Pre-aliquoted into separate vials: low contamination risk.l Comprehensive protocol included.

2X PCR Master Mix Composition:l Taq DNA polymerase (recombinant) in Reaction Buffer: 0.05u/µll MgCl2: 4mMl dNTPs (dATP, dCTP, dGTP, dTTP): 0.4mM of each.

Stability of 2X PCR MasterMix. Amplification of 950 bpsingle copy gene from 0.1µghuman genomic DNA using2X PCR Master Mix in 50µlreaction mixture.M - GeneRuler™ 100bp DNALadder Plus1 - Classical PCR with 1.25uTaq DNA Polymerase2 - 2X PCR Master Mix (-20°C)3 - 2X PCR Master Mix (after15 freeze-thaw cycles)

Features:l Fast - disruption of up to 192 samples in as little as 2-4 minutesl Cross-contamination-free - closed system prevents tube-to-tube

carryoverl Reproducible - highly standardized disruption methodsl Flexible - compatible with different sample types and disruption

buffersl Integrated - part of QIAGEN’s tissue-management system from

collection/stabilization to purification

Applications

The TissueLyser is well suited for high-throughput processing of animal

tissues. This is in contrast to rotor–stator homogenization, where samples

must be processed individually, and the rotor–stator homogenizer must

be cleaned after each sample to prevent cross-contamination.

Rat liver, lung, spleen, and kidney tissue was stabilized inRNAlater RNA Stabilization Reagent for 1 year at –20°C andthen processed in lysis buffer using theTissueLyser. TotalRNA was purified using the RNeasy Mini Kit and analyzedby formaldehyde agarose electrophoresis.

TissueLyser SystemFor high-throughput disruption of awide range of biological samples

TissueLyser withTissueLyser AdapterSet (96-well)

TissueLyser AdapterSet (24-tube)

Liver Lung Spleen Kidney

NEW


5


A Three in one kitTRI Reagent® Now available from Ambion

This is a complete, ready-to-use reagent for the isolation of total

RNA or the simultaneous isolation of RNA, DNA, and protein from

a variety of biological samples. Starting material can be either fresh

or frozen tissue, or cell samples of almost any size. It is an

economical alternative to the use of nucleic acid isolation kits.

l Simple—A single reagent for isolation of DNA-free RNA, RNA-

free DNA, and proteinl Productive—Obtain higher yields than with traditional

guanidine thiocyanate or cesium chloride methodsl Versatile—Works with many sample sources, including

human, plant, yeast, bacterial, and viral samplesl Flexible—Easily scalable depending on starting material and

desired yieldl Efficient—Samples processed in about 1 hour

Starting Material RNA Yield

Liver tissue 6-10 µg/mg tissue

Spleen tissue 6-10 µg/mg tissue

Kidney tissue 3-4 µg/mg tissue

Skeletal muscle or brain tissue 1-1.5 µg/mg tissue

Placenta 1-4 µg/mg tissue

Cultured epithelial cells 8-15 µg/106 cells

Fibroblasts 5-7 µg/106 cells

Target Preparation for Affymetrix GeneChip Analysis

New!—MessageAmp™ II-Biotin Enhanced Single

Round aRNA Amplification Kit

Features/Benefits

l Detect up to 9% more genes due to increase in Percent Present Calls

l Up to 10,000 fold amplification from just a single round

l Single round of amplification from as little as 50 ng of input RNA

l Optimized NTP mix containing biotinylated UTP reduces set up time and

maximizes labeling

l Highly reproducible results

l Reduce reaction costs by as much as 45%

Optimized IVT reaction, which includes, Bio-11-UTP in a new NTP mixes

l Obtain sufficient amount of amplified RNA from a single round of

amplification with only 50 ng of input RNA

l Biotinylated nucleotide included in the optimized NTP mix, reducing set

up time and maximizing labeling

l MegaScript® IVT reaction ensures optimal representation of the gene

expression profile

Higher Yields from as Little as 50 ng Input RNA

Optimization of the IVT step of the MessageAmpII-Biotin Kit protocol produced significantly higheryields after both a 14 h (overnight) and 4 h IVTreaction (single round of amplification) than thoseobtained with Company A’s system. Amplificationof just 50 ng of input RNA after a 14 hr IVTreaction yields enough cRNA (aRNA) for use onan Affymetrix GeneChip®. All reactions wereperformed in triplicate.

Downstream application:

RT-PCR, amplification for array analysis,

hybridization assays, or in vitro translation.

PCR, Southern blotting, and cloning. Isolated

proteins can be used in SDS-PAGE and

Western blots.

50 ng

Input

Single Round

Amplification

GeneChip

Analysis

aRNA Yield from Nine Different Sources. Four different amount of totalRNA from nine different sample types were amplified using theMessageAmpTM II-Biotin Enhanced Kit (Cat# 1791). Average aRNA yieldfrom triplicate reactions are shown for 4 or 14 hr in vitro transcription (IVT)reactions. Note that there is a ~3-fold difference in aRNA yield betweensome samples. With most RNA sources. 50-100 ng of input total RNAamplified with the MassageAmp II-Biotin Enhanced Kit using a 14 hr IVTincubation will yield enough labeled aRNA for microarray hybridization

What’s the Difference?

The MessageAmp II-Biotin Enhanced Kit

incorporates several improvements to the

in vitro transcription (IVT) step of the

MessageAmp II protocol. First the IVT

reaction has been optimized for the highest

yield of biotinylated amplified RNA (aRNA).

The most significant enhancement is

observed at the 50-100 ng input level where

you can obtain enough aRNA for a

GeneChip hybridization after one round of

amplification. This is a 20-50% increase in

yield over MessageAmp II.

Also for high yields, ease-of-use, and cost

savings, a biotinylated nucleotide has been

incorporated into an optimized NTP mix

for the new MessageAmp II Biotin

Enchanced kit.

V

RecoverAll™ Total Nucleic Acid

Isolation Kit for FFPEl Optimized for isolation of total nucleic acids, including microRNAs,

from FFPE tissuel No overnight Proteinase K digestion required—deparaffinize in

the morning and perform qRT-PCR in the afternoonl Obtain typical yields of >50% that of unfixed tissue from the same

sample sourcel Recovered nucleic acids are suitable for qRT-PCR, qPCR, mutation

screening, and microarray analysis

Deparaffinize in the Morning, Perform qRT-PCR in the Afternoon

Maximize Yield from Archived and Freshly Fixed Tissue

Gene Expression from FFPE Samples

Isolating DNA from FFPE Samples

Obtain microRNA for Expression Profiling

Yield of RNA from Archived Human FFPE TissueSamples: RecoverAll™ vs. Two Competitor SystemsA 10–20 µm section from each of the abovearchived human tissue blocks was isolated usingeach of three kits: Competitor A (red), Competitor Bkit (blue), and Ambion's RecoverAll Kit (green).Colon, lung, and breast samples were 1–2 yearsold; kidney was 3–5 years old; and bladder was 10–15 years old. Once the RNA was isolated, theconcentration was determined via OD260, and theamount of RNA recovered in µg was calculated. TheRecoverAll Kit yielded the highest recovery of thethree systems for all

Cells-to-Signal™ KitFrom Cells to qRT-PCR in just 5

minutes!

Save time and money with the rapid,

room temp. lysis procedurer Quantitate gene expression in cells — without isolating RNA

r 5 minute cell lysis procedure

r qRT-PCR from as few as 3 cells

r Ideal for monitoring siRNA-induced knockdown

r Easily adapted to high throughput analysis

The Cells-to-Signal Kits contain reagents for 30 or 100 reverse

transcription reactions, including M-MLV RT, oligo(dT)18 primers,

and random decamer primers. Up to 5 x 104 cells per reaction

can be used for the lysis step; 30% of the lysate can then be

used in the RT reaction.

qRT-PCR Detection of GSK-3α Using TaqMan® GeneExpression Assays and Following Cells-to-Signal™ ProtocolHeLa cells were lysed at concentrations of 1000, 200, 40, 8,and 1.6 cells/µl according to the Cells-to-Signal lysisprocedure. 6 µl of cell lysate were used in a 20 µl one-stepRT-PCR using a TaqMan Gene Expression Assay specific forGSK-3α.

LeukoLOCK™ Total RNA Isolation System

The LeukoLOCK™ Total RNA Isolation System is an innovative method for fractionation, stabilization, and extraction

of total RNA from the leukocyte population of whole blood. The LeukoLOCK System incorporates a leukocyte

depletion filter technology to isolate leukocytes from whole blood and Ambion’s RNAlater to stabilize the cells on

the filter. The RNA that is purified from captured leukocytes is inherently depleted of globin mRNA, improving its

utility for expression profiling and other applications

Features and Benefitsl A twenty fold reduction in absolute levels of alpha and beta globin mRNAsl Stabilization of the global expression profilel Eliminates the need to do an additional globin reduction protocol (enzymatic or otherwise)l Provides high quality RNA (1.8-2.1 A260/280 ratios)l Obtain an increase in percent present calls compared to whole bloodl Gives yields of 10-20 µg per 9 ml of blood

Application

RNA isolated from whole blood using the LeukoLOCK system is suitable for both end point and real time RT-PCR as

well as other downstream applications such as Northern blots, and ribonuclease protection assays. In addition,

highly reproducible results after amplification and array analysis were obtained from RNA isolated using the

LeukoLOCK system.

Ambions first kit for purifying WBC populations

Total RNA was isolated from whole blood using the LeukoLOCKSystem. Fractionated WBCs were processed either immediately(Day 0) or after 3 days of storage on the LeukoLOCK filter inRNAlater. The RNA was analyzed in real-time one-step RT-PCRusing MessageSensor RT conditions. Note the increase in Ctvalues presented for globin targets compared to whole blood, andthe stability of the Ct values for the remaining targets in Day 0 vsDay 3 RNA.

Improve Expression Profiles from Blood

GLOBINclear™– mRNA Removal from

Human, Mouse & Rat Samples

GLOBINclearTM Systeml Remove up to 95% of globin mRNA from whole blood total RNAl Detect up to 50% more genes—analyze previously undetected genesl Achieve greater representation of the genome, low 3'/5' ratiosl Avoid RNase H treatments that can degrade mRNA and alter expression profilesl Incorporate procedure with any RNA labeling protocol, including Ambion’s MessageAmp™ II aRNA Amplification

System

It is widely known that expression array data generated from whole blood total RNA samples have reduced detection

sensitivity compared to data from fractionated blood samples. Examination of whole blood total RNA reveals a

Increased Sensitivity with GLOBINclear™-Mouse/RatTriplicate GLOBINclear-Mouse/Rat reactions were performedwith pooled total RNA samples derived from either mouse orrat blood. The processed RNA was then amplified withMessageAmp II-96 to synthesize biotinylated aRNA probe forGeneChip® array analysis. Biotinylated aRNA was thenhybridized to triplicate Affymetrix microarrays (Mouse430A_2or Rat230_2 GeneChips). GLOBINclear processing resulted inan increase in genes, 46% & 56% for rat and mouserespectively.

dominant band in the 600-700 base region. This band

represents globin mRNA, which is expressed at high

levels in red blood cells (RBCs) and reticulocytes (RBC

precursors). Up to 70% of the mRNA (by mass) in whole

blood total RNA is globin transcripts.

Ambion offers a solution to this problem with

GLOBINclear. The GLOBINclear System employs a

novel, non-enzymatic technology that rapidly depletes

up to 95% of the alpha and beta globin mRNA from

total RNA preparations derived from whole blood. The

kit employs a novel hybridization technology that takes

advantage of the strength of biotin/streptavidin binding,

the specificity of nucleic acid hybridization, and the

convenience of magnetic separations

DDNA labeling using Biotin

ULS® reagent. The ULS®reagent binds to N7 of purineresidues to provide a stablecoordination complex betweenthe nucleic acid and the label(biotin or fluorescein).

Sensitivity of detection of labeled DNALabeled lambda/HindIII was used as a hybridization probe in a dot blot ofhomologous DNA (Nylon membrane)a- labeling with Fluorescein ULS Labelling kit, detection with Anti-fluocein-

AP, Fab fragments in combination with the chromogenic detection (NBT/BCIP)

b- labeling with Biotin ULS Labelling kit, detection with Straptavidin-Alkalinephosphate in combination with the chromogenic detection (NBT/BCIP).

Biotin ULS® LABELING and FLUORESCEIN

ULS LABELING KITS :Non-radioactive chemicals labeling of nucleic acids. It uses the Universal Linkage System (ULS®) technique,

which is based on the stable coordinative binding of a platinum complex to nucleic acids. The platinum complex

acts as a linker between a detectable marker (label) molecule, i.e. fluorescein or biotin, and DNA or RNA.

Featuresl One-step reaction.l Fast - only 30min to label the target.l Universal - any nucleic acid, independent of size or structure can be labeled.l Easy to scale up and down. It allows labeling of as little as 25ng or as much as 10µg of nucleic acid in a

single reaction.

Applicationsl For non-radioactive, non-enzymatic direct chemical labeling

of any nucleic acid with biotin (Biotin ULS® Labeling Kit) or

fluorescein (Fluorescein ULS® Labeling Kit):l Single-stranded and double-stranded DNA;l linear or supercoiled DNAs;l oligonucleotides;

l PCR products;

l chromosomes,

l DNA/RNA markers, RNA, PNA;

l siRNA.

l Labeled nucleic acids can be used in:

l spot blot analysis;

l dot blot hybridization;

l Southern blot analysis;

l Northern blot analysis;

l in situ hybridizations;

l comparative genomic hybridization.

NEW

NEW

NEW

The LeukoLockTM kit seperated into two parts-one containing theStabilization and Seperation reagents and the other containing the Isolationreagents. We are offering the following kits in addition to the origional kits.The LeukoLOCKTM Total RNA Isolation Kit Cat. No. 1933

The LeukoLOCKTM Fractionation and Stabilization Kit Cat. No. 1934

NEW

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6


Tosoh BioscienceBiochromotography Scenario

TSK-GEL 5PWprepacked columns

TSK-GEL 5PW Bulksemi-prep. scale

(20, 30 µm av. particle size)

Toyopearl prep(35, 65, 100, 200 µm av.

particle size)

Application support Application and registrationsupport

registration support

continous selectivity for easy scale up

Operating Volume 2 - 200 ml

Column ID 5 - 20 mm

Column length 40 + 20 cm

1 - 50 I

100 - 200 mm

40 + 20 cm

100 - 500 I

> 400 mm

40 + 20 cm

Leading provider and manufacturer of diagnostic test

components for detection of

SARS, HIV, HCV, HBV, HAV,

HDV, Syphilis and other

infectious agents.

NEW Products from VIROGENanti-Glutathione mAb D3

anti-GSK3B mAb 1H8

anti-FAS mAb 5F7

anti-FAS IgM mAb IPO-4

Induces apoptosis better then CH11

anti-CDw150 mAb IPO-3

anti-CD37 mAb IPO-24

anti-p53 mAb BP53-12

anti-CD3 mAb RIV9

anti-CD8 mAb RIV11

anti-CD16 mAb ASH 1975

anti-CD20 mAb B9E9

anti-CD22 mAb MYG13

Spectra/Por® dialysis tubing from

Spectrum Laboratories®.Spectra/Por® dialysis tubing is made of either regenerated cellulose

(RC) or cellulose ester (CE). Cellulose has long been used for dialysis

as it is uncharged and does not readily

absorb solutes. Further, the selectivity of

cellulose membranes is not altered greatly

by many chemicals or reasonable pH and

temperature ranges. Processed cellulose

has crystalline regions and these regions

cross-link chains to introduce structural

integrity to the cellulose. Depending upon

how the cellulose is processed the number

of crystalline areas varies and the resultant

regions between the cross-links can act

like size-selective pores.

Many Spectra/Por® membranes are

available with molecular weight cut offs

(MWCO) from as little as 0.1 kDa all the way up to 300 kDa (CE

membranes come in 0.1, 0.5, 1, 10, 100 and

300 kDa MWCOs, basic RC membranes

come in 3.5, 6–8 and 12–14 kDa MWCOs and

more expensive RC membranes have a range

of MWCOs from 1–50 kDa). MWCO values

are established by Spectrum® and represent

the size at which a solute is 90 % retain during

a test period.

Spectra/Por®

RC Dialysis Membrane

Spectra/Por®

CE-Membrane

Spectra/Por® 2

Dialysis Membrane

MWCO-12,000 - 14,000

Spectra/Por®

Membrane Tubing

Spectra/Por® is

supplied in an

essentially ready-to-go

format, no boiling in

bicarbonate buffer is

needed. Spectra/Por®

1–6 merely need

soaking in distilled

water for 30 minutes

whereas other tubing is

supplied pre-hydrated

vAntibodiesv Apoptosisv Signal Transductionv Cyclooxygenasev Endocrinologyv Enzyme Immunoassayv Leukotrienesv Lipids Lipoxygenasev Neurochemistry

Cayman serves you infollowing areas

RNA Research Essentials!

RNAlater® Tissue Collection: RNA

Stabilization Solution

3 Simplifies sample collection -

one reagent immediately

inactivates RNases and

stabilizes RNA within tissues or

cells

3 More flexibility - no need to freeze

samples in liquid nitrogen or rush

samples back to the lab freezer

3 Eliminates the need to freeze and

grind most tissue samples

3 Perfect for tissue collection "in

the field"

3 Flexible tissue storage - RNA is

stable for 1 day at 37°C, 1 week

at 25°C, 1 month at 4°C or

indefinitely at -20°C

3 Compatible with many RNA

isolation procedures including all

of Ambion's RNA isolation kits

RNaseZap® RNase Decontamina-tion Solution

3 Completely removes RNase

contamination from glass and

plastic surfaces

3 RNaseZap excels at removing

high levels of RNase

contamination whereas similar

products fail

3 Proven effective at removing high

concentrations of dried-on

RNase A

3 Ideal for cleaning work surfaces,

pipetters, and equipment that

must be RNase-free

RNAlater® Tissue Collection: RNAStabilization Solution

RNase-free Tubes and Tips

3 Tubes have a positive seal design, withstands -90° to 120°C

3 Available in a variety of sizes

3 Non-stick microfuge tubes are available with a low binding surface

3 PCR Tubes are functionally tested by PCR

Now mailing tissue samples

from collection site is possible

Removes RNase contamina-

tion from glass and plastic

surfaces without autoclavingGuaranteed RNase- and DNase-free

New! 15 ml and 50 ml Conical Tubes

Ambion’s RNase-free Conical Tubes are provided assembled with green

screw caps. The tubes have a printed writing space and volume marks

on the sidewalls for convenience. These polypropylene tubes are sterile,

nuclease-free, nonpyrogenic, and can be autoclaved to 121°C.

Microfuge Tubes

RNase-free microfuge tubes are available in three sizes, and each is

marked with convenient graduations. Nonstick RNase-free tubes are

useful for handling low concentrations of RNA and/or DNA because they

minimize nonspecific binding to the tube.

Elution Tubes

These 2 ml tubes can accommodate RNAqueous® and

Poly(A)Purist•Espin cartridges.

Dome Cap PCR Tubes. The thin-walled, dome-cap PCR tubes allow

uniform contact with the thermal cycler temperature block resulting in

superior well-to-tube temperature transfer and rapid cycle times.

Frosted Lid PCR Tubes

The thin-walled, flat, frosted cap provides a suitable surface for detailed

labeling.

Barrier Tips

Ambion's barrier tips prevent cross-contamination of samples by means

of an aerosol filter. They are RNase- and DNase-free and are ideal for

handling RNA, PCR applications, and for working with radioisotopes or

toxic chemicals.

Tips are compatible with Eppendorf®, Finnpipet, Oxford Benchmate®,

Pipetman•E Proline®, Rainin, and Socorex pipettors.

3 DEPC and non-DEPC treated water

3 High quality

All water products are certified to be nuclease free.DEPC-treated water is autoclaved pre- and post-packaging to ensure sterility and inactivation ofDEPC; available in 100 ml, 500 ml and 1 liter sizes.

Certified nu-

clease free-water

is also available

with Ambion

Cat# 9930

Tubes have either

dome caps (0.5

ml size) or frosted

lids (0.2 and 0.5

ml sizes).

Tubes can

accomodate

RNAqueous®

spin cartridges.

15 ml and 50 ml

Conical Tubes

Marked with

graduations and

are available in

three sizes.

Blue-BANDit™

• No More Methanol and Acetic Acid

• Sensitivity Below 20 ng of Protein/Band

• Hands-Off! Simple Staining/Destaining

Procedure

AMRESCO’s Blue-BANDit™ stain is a conve-nient alternative

to traditional Coomassie® Blue staining procedures.

Staining results are clear and precise. Environmentally

friendly, this ready-to-use stain does not contain methanol

and ace-tic acid and does not require hazardous sol-vents

for destaining. Blue-BANDit™ staining exhibits sensitivity

below 20 ng of protein per band. Packaged as a 1X ready-

to-use solution, Blue-BANDit™ only requires water for the

prewashing and destaining steps.

PHOTO LEGEND: Results of an 8%

polyacryla-mide gel loaded with

serial dilutions of High Range Protein

Marker (40-212 kDa) using 1X TG-

SDS buffer and visualized with Blue-

BANDit TM (Gel A) and Standard

Coomassie® Blue G-250 (Gel B).

v Nitric Oxidev Nuclear Receptorsv Oxidative Injuryv Oxylipinsv Phospholipasev Photo Detectionv Prostaglandinsv Fatty Acidsv Steroids

and must be stored at 4 °C. One concern is that these membranes contain

trace amounts of heavy metals and sulfur although Spectrum® do also

produce specific cleaning solutions and supply Spectra/Por® 7 which

comes with minimal contaminants. Membranes can be sterilized and

also have two year shelf lives. Finally, Spectra/Por® tubing is supplied in

different widths depending upon the scale of dialysis you plan.

l Easy to usel Stable Surface chemistryl Compatible with automated microarray equipmentsl Low background fluorescencel Polymer slide for protein microarrays Ideal where very low

concentrations are to be quantifiedl Low background fluorescencel Shelf life 5 years Improved signal to noise ratio versus glass slidesl Batch to Batch Consistancy

Protein MicroArray SlidesThese slides are specifically desinded for protein

microarray. The protein molecules are bound to the

surface by a stable, non-covalent interaction. It is

not necessary to perform any protein modification

steps before using the slides. The MaxiSorp surface

is applicable for many protein array, such as

antibody,antigen and peptide assays

Barcoded

slides available

on request

NEW

96 CytoWell™ Plates

for cell based assayOptical Bottom

l Black cell culture plates with transparentbottoms.

l Excellent optical quality.l Thin 50 µm film bottoms for high.

magnification and resolution microscopyand imaging.

l Flat bottoms across the plates facilitateautomatic imaging.

l Flatness conforms with requirements forconfocal microscopy.

l High gas transfer rates facilitate cellculture of fastidious cells.

l Surface treatment ensures uniform, stablecell attachment and growth.

l Very low auto fluorescence.l UV-transparent.l 96 well footprint compatible with standard

equipment and automated systems.

Robust high performance plates

for automated handling and imaging

Also available in 384 Well Plate

format from January 2006

Literature Available on Request

1 Lineage tracing and Charactrization of

insulin-secreting cells guarated from

adult pancreatic aciner cells.

1 Large-Scale Production of Recombinant

Viruses by Use of a Large Culture

Vessels with Active Gassing.

NEW


7


Vivapure - the next step in protein purificationVivascience Protein Purification The separation of pure proteins from complex mixtures is a key process in biomedical research and other biological disciplines. Vivascience offers Vivapure spin

columns based on an innovative and powerful membrane adsorber technology for the purification of proteins.

Vivapure AdenoPACK™ 500 - for Adenovirus

purification and concentration

Kit for adenovirus purification and concentration : In only 2 hours 3 x 1013 purified virus particles are recovered

from 500 ml cell culture. This easy to use kit replaces lengthy and inefficient cesium chloride density gradient

methods and is the most cost-effective kit in the market for purifying 3 x 1013 viral particles

Handling overview - Purified virus in about 2 hours

Add Benzonase

and clarify viralsupernatant

Load clearedviral supernatant

Wash Elute Concentrate

Membrane adsorber technology:

Membrane adsorbers are ideal for virus purification. Large flow

through pores allows unrestricted adenovirus access and

convective transport speeds purification.

Fluorescent cell assay

Photography: kindly provided by Dr. Lux, University Hospital and

University of Applied Sciences, Mannheim. COS-1 cells infected

with Ad5 GFP-constructs after purification and concentration

with Vivapure AdenoPACK 100.

The Vivapure Advantage

l Rapid 2 hour purification protocol

l Fast recovery of 1 - 5 x 1012 VP from 500 ml cell culture

l Easy to use

l Non-toxic buffers

l Fast

l Cost effective

l Centrifuge based

l Parallel processing

l Reproducible

Available in a range of different membrane chemistries

(e.g. range of strong or weak ion exchangers, metal chelate, Protein A, epoxy)

Vivapure Anti-HSA/IgG Kit for Human Albumin and IgG Depletion

Highly specific human albumin and IgG depletion with unique antibody fragments and Protein G

Reveal your proteome - see previously undetectable proteins

Handling overview - Albumin and Albumin/igG removal in 20 minutes

Fill Vivaclearwith Anti-HSA.IgG

Affinity Resin

Mix. sample with Anti-HSA/igG Affinity Resin

incubate for 15 min.

Recover albuminand igG depleted

sample

Wash 1 - 3x with BindingBuffer for

complete samplerecovery

Albumin and IgGdepleted sample isready for analysis

l Highly specific antibody fragments >95% albumin removal from human serum

l No cross reactivity with other human proteins

l Compatible with all downstream proteomics applications due to low salt

concentrations in the buffer

l Fast 20 min. protocol

l Easy-to-use spin column based protocol for parallel processing

l Flexibility in sample volume to be depleted

Human serum was analysed before and after albumin/IgG removal by IEF and 2D PAGE. The comparison of the two

gels clearly demonstrates the increased resolution, leading to the visualisation of lower abundance proteins achieved

with the Vivapure Anti-HSA /IgG Kit.

Vivapure Anti-HSA/IgG Kit Advantages

Antibody based albumin removal Efficient albumin removal with minimal loss of

potential serum biomarkers

Protein G based IgG removal Efficient IgG removal. Also applicable to mouse and

rat samples

20 minute protocol employing spin tubes Quick, suitable for parallel processing

Depleted protein recovered in low salt buffer Sample is ready for next step in fractionation process

Resin packaged as a slurry Flexibility in serum sample volumes

Kit format Ready to use, no wastage

Priced for single use No risk of cross contamination

New&

Improved

Vivapure® C18 Micro spin columns - Improved sample Newpreparation for mass spectrometry:

It contain an innovative C18 membrane and are easily handled in the centrifuge. They are ideally suited for

concentration, purification and desalting of peptides prior to analysis.

Vivapure® C18 - Benefits

l Easy centrifugal procedure

l Minimal hands on time

l High loading capacity (up to 200 µl)

l Low elution volume (3 µl)

l Direct elution with matrix possible

l Highly reproducible

l Convenient parallel processing

Desalting and sample concentration with Vivapure® C18 Micro spin columns gave rise

to a better resolution and an increased number of signals suitable for protein dentification

Handling overview

Equilibrate Bind Wash Elute Purified sample

New

Vivapure Epoxy Protein Coupling Kit - Create

your own affinity matrix for purifying your desired

protein

Coupling affinity ligands with epoxy groups

The Vivapure Epoxy Protein Coupling Kit is a new tool for protein

immobilization for creating any desired affinity spin column. This ready-to-use

kit utilizes an epoxy membrane adsorber that is fitted into a spin column for easy

and quick handling

Immobilization of proteins. Amino acids possessing either amino-, hydroxyl- or

thiol-groups can be immobilized with the epoxy group.

The Vivapure Advantagel Fast results - only 3 hours couplingl Mild coupling conditionsl Ready-to-use kit

Handling overview

CustomProteinSample

Dilute 1:1withBuffer A

EquilibrateMembrane

LoadDiluteProteinsample

flow-through

Pipetteflowthroughontomembrane;incubate 3hr at RT

Wash Customproteincoupled toVivapureEpoxy MiniSpin Column

New

Vivacell 100 utilizes:l Pressurel Centrifugel Pressure-shake

Vivacell 100

20 - 100 ml samples

Vivacell 100 is the latest member of the Vivacell family and bridges the volume range between

the Vivacell 70 and the Vivacell 250. The patented vertical membrane design allows highest

performance and unmatched flexibility. Vivacell 100 is a unique and

innovative concentrator for volumes from 20 ml to 100 ml, which

utilizes pressure, centrifuge or pressure-shake to rapidly concentrate

even samples with very high particle loading. Vivacell 100 is designed

for centrifugal concentration of samples up to 100 ml which makes

it the largest centrifugal unit available. At the same time, the new

construction design allows for maximum centrifugal force of 4,000xg

to be used for even faster concentration.

Vivaspin 15R

2 - 15 ml samples

Vivaspin 15R is the latest member of the Vivaspin product family with all

the unique features of Vivascience concentrators ncluding a patented

vertical membrane and a dead stop. Vivaspin 15R is targeting the volume

segment 2 to 15 ml with a modified regenerated cellulose membrane;

Hydrosart®. This membrane is ideal where extremely high recovery with

very low adsorption is needed, for example in applications such as desalting

and concentration of Ig fractions.

l Ultimate recovery at low Adsorption (95-98%)

l Extremely short concentration time (30x in 15 min.)

l Convenient application protocol with easy handling

l Easy scale-up to Vivaflow 200 with Hydrosart® membrane for

volumes up to 5 litres

l Very small hold up volume (< 20 µl)

Spin Recover

Direct

upscale of

AdenoPACK 100

NEW

NEW

NEW

NEW

NEW


8


Etest® trademark now enjoys internationalrecognition and It is the first in a new generationof novel and patented products for Anti-microbialResistance Testing.Etest comprises a predefined gradient ofantibiotic concentrations on a plastic strip.It is used to determine the exact MinimumInhibitory Concentration (MIC) of antibiotics,antifungal agents and antimycobacterial agents,in µµµµµg/ml.It is a cost-effective tool for generating MICsacross 15 dilutions. It can also be used in amacromethod format to optimise resistancedetection. Over 100 anti-biotics are nowavailable in the product range for testing of

aerobic bacteria and fastidious organisms. Etest promotes the rationaluse of antibiotics by providing accurate results to guide the therapy ofindividual patients and to validate empiric drug regimens on a unit specificbasis. It is also widely used in resistance surveillances and clinical trials.

Media for the antibiotic testing varies fromorganism to organism

Configuration of Epsilometer

The Etest comprises of a thin impervious test car-

rier (5x50) with a continuous exponential gradi-

ent of antibiotics immobilized on one side and a

reading and interpretive scale on the other side.

The antibiotic gradient can cover a broad con-

centration range

corresponding to

approximately 20

MIC two-fold dilu-

tions. The slopes

and concentration

ranges can be op-

timally designed to

correspond to clini-

cally relevant MIC

ranges and break-

points selected for

categorization of

s u s c e p t i b i l i t y

groups.

Epsilometer test Principle: The antibiotic gradi-

ent is applied onto the surface of an inoculated

agar plate. After the required period of incuba-

tion whereby bacterial growth becomes visible an

inhibition ellipse is seen. The zone edge intersects

the lenth of the graded carrier at a position where

a specific concentration of the antibiotic causes

an inhibition and cessation of bacterial growth.

The value named the inhibitory concentration (IC)

is a direct measure of the susceptibility of the

micro organism for the particular antibiotic.

Storage and Handling of Etest Strips

1. Etest strips are supplied in a plastic blister pack-

age with 10 units in

each of 10 individu-

ally sealed com-

partments. Each

package contains

100 units of one an-

tibiotic.

2. All packages, in-

cluding unopened blister compartments, must

be stored in a freezer at -20°C until the expiry

date.

3. Always store unused strips in airtight contain-

ers e.g. storage tube with silica gel. Ensure that

the desiccant is blue during storage and be-

fore use.

4. Reactivate the desiccant when it turns light blue

or white, remove the cap, replace the

Styrofoam with a plug of cotton wool and heat

the tube to 160oC for

one hr. cool the tube,

replace the cap and

store in dry place.

5. Mark the storage

tube with the label

from the blister

pack,showing the expiry date,batch no and

antibiotic code.

6. Store the tube at –20oc and always allow it to

reach room temprature before opening.

7. If stored at –70oc,leave the tubes in the

Styrofoam outer packaging during equilibra-

tion to room temprature to prevent the caps

from popping out of the tubes.

8. Strips in airtight storage tubes have the same

expiry date as the origional package, if prop-

AB BIODISK develops new product concepts and processes for manufacturing unique high quality diagnostic tests. The company’sproprietary technology enables biochemical reagents to be accurately dispensed in picogram quantities in stable dry-chemistryformats. This innovative technique is used to produce antibiotic gradients for products like Etest®, System.

erly stored.handled

and always kept dry.

Application

Open the package us-

ing forceps or your fin-

gers, remove the re-

quired number of Etest

strips and place them on a dry clean surface e.g.

a petri-dish or in the Etest applicator tray Use a

manual applicator, vacuum pen or forceps to ap-

ply the strip to the inoculated agar surface with

the MIC scale facing upwards (towards the

opening of the plate) and the handle at the rim of

the plate. Ensure the whole strip is in complete

contact with the agar surface. Do not place the

strip upside down as no inhibition ellipse will form

because the antibiotic

will not diffuse across

the non-porous plastic

strip. If air pockets are

seen under the strip, re-

move them by pressing

gently on the strip (with-

out moving it) with forceps or an applicator stick

moving from the low concentration upwards.

Small bubbles will not affect results. Once ap-

plied, the strip cannot be moved because of

instantaneous release of antibiotic into the

agar.

Epsilometer test procedure: The procedure for

using the Etest for antimicrobial susceptibility test-

ing consists of three major steps.

An agar plate with a suitable test medium is in-

oculated according to standard practice. This may

include flooding swabbing or seeding the test

plate with the microorganism. The inoculum used

may be a suspension of an overnight culture, the

so-called ICS inoculum, or a 0.5 McFarland sus-

pension of a log phase culture. Test carriers are

then applied in an optimal pattern as shown in fig

3 with the concentration maxima nearest the cir-

cumference of the petridish. The plate is immedi-

ately incubated in the required atmosphere for

15-18hrs. After incubation inhibitory concentra-

tion are directly read from the graded test carri-

ers. Results can be reported as a quantitative

value, i.e. an inhibitory concentration of x µg/ml

and or a susceptibility group as defined by spe-

cific MIC/IC correlated break points.

Product µµµµµg/ml Cat No.

Amikacin 0.016-256 AB5100 0138

Amoxicillin 0.016-256 AB5100 0098

Amoxicillin/clavulanic acid (2/1) 0.016-256 AB5100 0108

Ampicillin 0.016-256 AB5100 0158

Ampicillin/sulbactam (2/1) 0.016-256 AB5100 0188

Azithromycin 0.016-256 AB5100 0168

Aztreonam 0.016-256 AB5100 0178

Bacitracin 0.016-256 AB5100 2868

Benzylpenicillin (high range) 0.016-256 AB5100 0258

Benzylpenicillin (low range) 0.002-32 AB5100 0268

Cefaclor 0.016-256 AB5100 0458

Cefalexin 0.016-256 AB5100 3018

Cefdinir 0.016-256 AB5100 2888

Cefditoren 0.002-32 AB5100 3448

Cefepime (high range) 0.016-256 AB5100 0508

Cefepime (low range) 0.002-32 AB5100 0498

Cefixime 0.016-256 AB5100 2998

Cefodizime 0.016-256 AB5100 0518

Cefoperazone 0.016-256 AB5100 2958

Cefoperazone/sulbactam (2/1) 0.016-256 AB5100 2938

Cefotaxime (high range) 0.016-256 AB5100 0558

Cefotaxime (low range) 0.002-32 AB5100 0568

Cefotetan 0.016-256 AB5100 0638

Cefoxitin 0.016-256 AB5100 0658

Cefpirome 0.016-256 AB5100 0648

Cefpodoxime 0.016-256 AB5100 0588

Cefprozil 0.016-256 AB5100 2668

Ceftazidime 0.016-256 AB5100 0678

Ceftibuten 0.016-256 AB5100 0718

Ceftizoxime (high range) 0.016-256 AB5100 2658

Ceftizoxime (low range) 0.002-32 AB5100 2738

Ceftriaxone (high range) 0.016-256 AB5100 0668

Ceftriaxone (low range) 0.002-32 AB5100 0708

Cefuroxime 0.016-256 AB5100 0698

Cephalothin 0.016-256 AB5100 0358

Chloramphenicol 0.016-256 AB5100 0758

Ciprofloxacin 0.002-32 AB5100 0868

Product µµµµµg/ml Cat No.Amphotericin B 0.002-32 AB5100 2638

Flucytosine 0.002-32 AB5100 1098

Fluconazole 0.016-256 AB5100 1088

Itraconazole 0.002-32 AB5100 2588

Ketoconazole 0.002-32 AB5100 2598

Voriconazole 0.002-32 AB5100 3288

Etest® For Antifungal

ETEST ESBL FOR CONFORMATION OF EXTENDED SPECTRUM BETA-LACTAMASE

Product µµµµµ/ml Cat. No.

Cefotaxime/cefotaxime + clavulanic acid (4µ/mL) 0.25-16/0.016-1 AB5100 3228Ceftazidime/ceftazidime + clavulanic acid (4µg/mL) 0.5-32/0.064-4 AB5100 3258Cefepime/cefepime + clavulanic acid (4µg/mL) 0.25-16/0.064-4 AB5100 3478ETEST MBL FOR DETECTION OF METALLO BETA-LACTAMASE

Imipenem/imipenem + EDTA 4-256/1-64 AB5100 3428

Etest® For Antimycobacterial

Clarithromycin 0.016-256 AB5100 0878

Clindamycin 0.016-256 AB5100 0958

Colistin 0.064-1024 AB5100 3338

Doxycycline 0.016-256 AB5100 0978

Enrofloxacin 0.002-32 AB5100 2898

Ertapenem 0.002-32 AB5100 3168

Erythromycin 0.016-256 AB5100 1058

Ethambutol 0.016-256 AB5100 2778

Ethionamide 0.016-256 AB5100 2758

Fosfomycin 0.064-1024 AB5100 2918

Fusidic acid 0.016-256 AB5100 1158

Gatifloxacin 0.002-32 AB5100 3028

Gentamicin (high range) 0.064-1024 AB5100 1278

Gentamicin (low range) 0.016-256 AB5100 1258

Imipenem 0.002-32 AB5100 1368

Isoniazide 0.016-256 AB5100 2798

Kanamycin 0.016-256 AB5100 2788

Levofloxacin 0.002-32 AB5100 2748

Linezolid 0.016-256 AB5100 3138

Mecillinam 0.016-256 AB5100 1378

Meropenem 0.002-32 AB5100 1388

Metronidazole 0.016-256 AB5100 3008

Minocycline 0.016-256 AB5100 1608

Moxifloxacin 0.002-32 AB5100 2908

Mupirocin 0.064-1024 AB5100 1638

Nalidixic acid 0.016-256 AB5100 1658

Netilmicin 0.016-256 AB5100 1758

Nitrofurantoin 0.032-512 AB5100 3048

Norfloxacin 0.016-256 AB5100 1958

Ofloxacin 0.002-32 AB5100 1968

Oxacillin 0.016-256 AB5100 2058

Pefloxacin 0.016-256 AB5100 2088

Phenoxymethylpenicillin 0.016-256 AB5100 2138

Piperacillin 0.016-256 AB5100 2158

Piperacillin/tazobactam (4 ¼g/mL) 0.016-256 AB5100 2148

Polymyxin 0.064-1024 AB5100 3348

Quinupristin/dalfopristin 0.002-32 AB5100 2878

Rifampicin (high range) 0.016-256 AB5100 2178

Rifampicin (low range) 0.002-32 AB5100 2608

Roxithromycin 0.016-256 AB5100 2648

Sparfloxacin 0.002-32 AB5100 2578

Spectinomycin 0.064-1024 AB5100 2928

Streptomycin (high range) 0.064-1024 AB5100 2688

Streptomycin (low range) 0.016-256 AB5100 2188

Sulfadiazine 0.064-1024 AB5100 3438

Sulfamethoxazole 0.064-1024 AB5100 3418

Teicoplanin 0.016-256 AB5100 2208

Tetracycline 0.016-256 AB5100 2258

Ticarcillin 0.016-256 AB5100 2238

Ticarcillin/clavulanic acid (2 ¼g/mL) 0.016-256 AB5100 2268

Tobramycin (high range) 0.064-1024 AB5100 3318

Tobramycin (low range) 0.016-256 AB5100 2278

Trimethoprim 0.002-32 AB5100 2368

Trimethoprim/sulfamethoxazole (1/19) 0.002-32 AB5100 2448

Vancomycin 0.016-256 AB5100 2558

Meet with Next Generation Cryo Tubes

FEATURESl The vials are clicked into the low profile micoplate format rack to

allow for automatic manipulation and automated transport without

the risk of loosing samples

l The cap is designed with a socket to allow automated manipulation

through a click of the screwdriver into the cap

l Designed for low temperature storage of biological materials

l Developed for automated handling

l Dense storage format for optimized freezer utilization

l Working volume of 0.5 or 1.0 ml

l CryoBank™ low protein adsorption surface for storage of cells and

proteins

l Bank-It™ for low DNA adsorption surface for storage of DNA

l Unique 14 x 14 array 2D Code

l CE-marked for diagnostic use

l Sterility assurance level 10-6 on the CryoBank vials

l DNAse/RNAse free

l Non-pyrogenic according to the LAL-test (only valid for CryoBank™)

l Non-toxic according to USP class VI test

l Conform to IATA requirements for the transport of diagnostic specimens, UN packing instruction 602 and 650

Innovative Storage Technology forPharmagenomics / Cell Banking / Biobanks

CryoBank™ and Bank-It™

A range of accessories is provided to

facilitate easy handling and storage

Microplate rack

Nunc MicroPlate Storage

Racks in 4 different colours

for storage of CryoBankTM

or Bank-ItTM plates

Storage Box

White cardbord for

169 tubes

Scew Driver

For mannual unscrewing

and fastening of the caps

Handheld electric

scewing heads

The cap is prepared for cap

manipulation with a spring

ball bearing socket

The low binding surface used by Nunc ensures maximum sample recovery

Chose Products

of Your Interest

NEW


9


Multidish 4 for IVF Polystyrene. With LidMouse embryo tested Ask for Cat.No 144444

Please observe that the Cat.No 176740 is not mouse embryo testedl Intended use of this 4 well dish is in vitro fertilizationl Same surface, design and packaging as Cat. No. 176740l CE marked according to Medical Devices Directive 93/42/EECl 510K, i.e. produced and marketed in compliance with the FDA

requirements for IVF productsl Full batch control of all components in the final product giving full

traceability

l Easy handling with one hand only Multidish 4 for IVF Nunclon™?

l Same surface, design and packaging as Cat. No. 176740

l CE marked according to Medical Devices Directive 93/42/EEC

l 510K, i.e. produced and marketed in compliance with the FDA

requirements for IVF products

l Full batch control of all components in the final product giving full traceability

l Easy handling with one hand only

l Unique certification based on a 1-cell stage mouse embryo toxicity test; the certificate confirms sterility (SAL 10-

6), non-pyrogenicity and that the material has passed USP class VI test

l The embryo toxicity test is a release test and the product will only be sold after it has passed the test

l Unique certification based on a 1-cell stage mouse embryo toxicity test; the certificate confirms sterility (SAL 10-

6), non-pyrogenicity and that the material has passed USP class VI test

l The embryo toxicity test is a release test and the product will only be sold after it has passed the test

150628

140685

140675

142475

142485

152035

152034

176600

150687

152640

176597

176740

Low Cell Binding Plates and DishesSterile. Polystyrene. With Lid

l Low Cell Binding surface is based on a polymer presenting a phosphorylcholine moiety to the

cells, thereby mimicking the surface of a cell membrane

l Biologically inert and biocompatible

Useful for:

l Culture of oocytes, e.g. Xenopus

l Colony assay for cancer cells

l Isolation of macrophages

l Detection of cytokines and other cell secretions

l Culture of embryonic bodies

l Culture of spheroids, e.g. HepG2

Applications

l Cell Culture

FluoroNunc™/LumiNunc™ Plates Nunclon™Polystyrene. Sterile. External dimensions 128 x 86 mm

l MicroWell™ Plates for fluorescence/ luminescence

based cell assaysl Nunclon™? certifiedl Plate edges and lids are designed to reduce

evaporationl Raised well rims reduce risk of cross contaminationl Fit standard equipmentl White plates for minimum autofluorescence and

autoluminescencel White plates give maximum reflectionl Black plates give minimum background in

fluorescence readingl Black plates allow minimum scattering of light

Applicationsl Cell Cloning Studiesl Cloningl Fluorescence Based Assays

Accessories for

Nunc Cell

Factories

LARGE-SCALE CELL CULTURE PRODUCTIONCell Factories for Active Gassing Nunclon™Length 335 m, width 205 mm

l Accessories for

filling and

emptying the Cell

Factories with

media

l Under a LAF-

Bench, the sterile

funnel may be

used

CELL FACTORY HAND MANIPULATOR FOR CF10 AND CF40l The Nunc Hand Manipulators are designed to manually handle a Cell Factory 10 with a growth area of 6,320

cm 2 respectively a Cell Factory 40 with a growth area of 25,280 cm 2l The CF10 Hand Manipulator comes as a tabletop model with firm base, while

l The CF40 Hand manipulator comes with wheels and a foot brake

BENEFITSl Low initial investment costsl Reduced lifetime operating costsl Reduced validation complexityl Low risk of cross-contaminationl Easy upscalingl Faster to market

EPA-TEC GEN. 9

Automatic Cell

Factory Manipulator

l The AFCM is an electronically and pneumatically

controlled unit which automates the filling and

emptying of medium or cell suspension into 4 x 3

Nunc Cell Factory 10 or 4 x 1 Nunc Cell Factory 40

l Included with the AFCM are 5 assembly racks with

carts and 1 standard set of spare parts

l For industrial scale production, for example

vaccines, monoclonal antibodies or

pharmaceuticalsl The patented Gas-flow system secures a

controlled atmosphere in the culture trays by equal

distribution of user-specified gas mix actively

pumped through the pre-mounted filterl Gas is equally distributed between trays as well

as in each individual trayl For culturing and nursing of cells via controlled

gas distribution - especially oxygen demanding

and pH-sensitive cells may benefit from the

controlled atmospherel Certified Nunclon™? surface treatment ensures

excellent conditions for cell attachment and growthl Growth kinetics unaltered from laboratory scale

culturel Available in 4, 10 and 40 tray versions for easy

scale-up, low contamination risk and Compact

designl Compatible with existing manually and automated

handling equipment from Nunc

Applicationsl Cell Culture, Large Scale Cell Culturel Monoclonal Antibody Productionl Upscaling

EPA-TEC Shakerl Serves to detach cells by performing continuous

horizontal acceleration and deceleration movements

to the Cell Factory/liquid systeml The frequency of the shaking movements can be

controlled through the operating panel. The Shaker

can either be directly controlled or preset by the timerl Built to hold a rack with 4 x 3 Nunc Cell Factory 10 or

4 x 1 Nunc Cell Factory 40

Shaker Length 2300 mmwidth 545 mmHeight 1140 mmWeight 360 kg

Operating Load PowerPanel Shake Timer1

Move1 Timer2, ch. AMove2 Timer2, ch. B

Stop Emergency StopElectric High Voltage AC 400 Volt/0.75KWCabinet Low Voltage AC/DC 24 Volt

Vacuum Pump

A Virtually Two Dimen-

sional.. 2D MicroHex™l Microscopic polystyrene non-porous

hexagons for adherent cell culturel Certified surface treatment ensures ex-

cellent condi-

tions for cell

at tachment

and growthl 2D MicroHex

can be kept

in suspen-

sion at low

stirring speed

due to the shape and low weight of the

particles. This facilitates the initial cell

attachment and results in a uniform cov-

erage of all carriersl 2D MicroHex are solid, non-swelling,

non-porous and non-absorbing par-

ticles. Therefore, no reagents can be

trapped in the carriers, as is the case

with gel particlesl 2D MicroHex have the well-known prop-

erties of Nunc cell culture plastics, in-

cluding easy trypsinization of cellsl Very low displacement volume/unit sur-

face area

Microcarriersfor large scale production

Barcoded CryoTubes™Pad-print/Ink Jet is done by printing directly on the tube

l Withstand temperature changes, like freezing in liquid nitrogen and

the subse-quent thawing

l Standard code Interleaved 2 of 5

l Available from 1.8 to 4.5 ml Label - the unique Nunc label withstands

many chemicals e.g. toluene, acetone and DMSO

l Withstand temperature changes like freezing and thawing

l Resistant to autoclaving

l Available as external and internal thread and from 1.0 to 4.5 ml

l Available with code 128, Interleaved 2 and 5 or code 39

Applications

l Cryogenic Storage

l Gas Phase Freezing

l Liquid Nitrogen Freezing

l Mechanical Freezing

l Sample Identification

NUNCVarious Competitors

L929 Cell Adsoption to CryoTube (3 days incubation)

NUNC V/s Competitors

NEW

NEW

NEWNEW


10


Streptavidin Passively Coated Plate and Module96 wells per frame. Polystyrene. External dimensions 128 x 86 mm

l Ideal for binding of biotinylated biomolecules, such as peptides, antibodies, oligonucleotides or haptens

l Streptavidin is passively coated on an area of 154 mm² (area covered by a volume of 200 µl)

l Binding capacity for biotin of at least 13 pmol/well*

l Stable at room temperature

l General coating protocol available on request

l Available in C96 plates or C8 well strips

Applications

l Covalent Immobilisation, ELISA

* Depending on size or sterical properties of a given biomolecule the actual moler binding

capacity might be different

Immobilizer™ Plates and

Modules Streptavidinl Instant coupling of biotinylated bio-moleculesl Streptavidin is covalently bound to the surfacel Ready to use - no blocking stepsl Excellent binding capacity (e.g. F96 clear 20 pmol/

well*)l Minimal surface leachingl Reaction site easily accessiblel Low non-specific bindingl High signal to noise ratiol Long shelf-life at room temperature (minimum 18

months)l Low reagent consumptionl White and black plates for luminescence or

fluorescence applications

l Depending on size or sterical properties of a givenbiomolecule the actual molar binding capacitymight be different.

Coupling of a biotinylated protein to the covalently bound

streptadivin. After a pre-wash, simply add the biotinylated target

molecule in an appropriate buffer. In a short incubation step, the

biotinylated molecule will bind to the streptavidin molecule.

Immobilizer™ Plates Nickel-Chelate96 wells per plate. External dimensions 128 x 86 mm

l For detection and quantification of 6xhistidine-tagged

fusion proteinsl Covalently coupled Nickel-Chelate on the surfacel No blocking stepsl Low detection limitl Low backgroundl High signal to noise ratiol Stable at room temperature (for minimum 1 year)l Low reagent consumptionl Reaction site easily accessiblel White and black plates for luminescence or


Applicationsl Covalent Immobilisation

Coupling of a 6xHIS tagged protein/peptide to the Nunc

Immobilizer Nickel-Chelate Plate.

His = histidine

Immobilizer™ Plates Glutathione96 wells per plate. External dimensions 128 x 86 mm

l For detection and quantification of GST-tagged fusion

proteinsl Covalently coupled glutathione on the surfacel Ready to use - no activation steps or blocking stepsl Low detection limitl Low background noisel High signal to noise ratiosl Stable at room temperature (1 year)l Low reagent consumptionl Reaction site easily accessiblel White and black plates for luminescence and


Applications

Covalent Immobilisation

Coupling of a GST-tagged protein/peptide to the Nunc

ImmobilizerTM, Glutathione Plates.

GSH = Glutathione

GST = Glutathione-S-transferase

Applicationsl Covalent Immobilisation

InVitro PETG Roller BottlesFor industrial scale production of vaccines, monoclonal

antibodies or pharmaceuticals Moulded of durable PETG

Excellent substrate for adherent cells Quick-action

ergonomic closure reduces wrist stain and increases

productivity Wide range of sizes with surface areas from

1050 cm² to 4200 cm² Available in both Standard and the

patented Expanded Surface (XPS) Roller Bottle Easy-to-

read graduations for medium fills

l For industrial scale production of vaccines, monoclonal

antibodies or pharmaceuticals

l Moulded of durable PETG

l Excellent substrate for adherent cells

l Quick-action ergonomic closure reduces wrist stain and

increases productivity

l Wide range of sizes with surface areas from 1050 cm²

to 4200 cm²

l Available in both Standard and the patented Expanded

Surface (XPS) Roller Bottle

l Easy-to-read graduations for medium fills

Recently Introduced

l Holds 24 ounces

l Weighs 121 grams

l Sports a sleek, slender profile

l Allows for easy one handed operation

l Opens and closes with flip-top lid to eliminate 99% of leaks

l Let’s you enjoy the flavors of your beverage, not your bottle

l 0025901024 is Slate Blue

l 0025902024 is Sage Green

l Extremely durable

l Resistant to staining

l Resistant to retaining odors

l Recommended for “extreme” adventures

l Dishwasher Safe (top rack only)

l Withstands temperatures from -135ºC (-211ºF) to 135ºC (275ºF)

OTGIntroducing the New

You asked for it, we created it - the new

“On the Go”

bottle from NALGENE!

Made with

Polycarbonate

The Nalgene OTG

ELISA DEVELOPMENT KITSHuman IL-4 ELISA KitHuman IL-8 ELISA KitHuman INF-gamma ELISA KitRat IL-2 ELISA KitRat IL-4 ELISA KitRat IL-10 ELISA KitRat IL-12p40 ELISA KitHuman PDGF-BB ELISA KitHuman EGF ELISA KitHuman FGF-basic ELISA KitHuman VEGF ELISA KitMurine IL-2 ELISA KitRat IFN-gamma ELISA Development KitHuman IL-1-alpha ELISA KitHuman IL-2 ELISA KitHuman GRO-beta ELISA KitMurine MIP-1-alpha ELISA KitMurine JE (MCP-1) ELISA KitHuman IL-3 ELISA KitHuman sRANKL ELISA kitHuman CD40 Ligand ELISA KitHuman TWEAK ELISA KitHuman IL-5 ELISA KitHuman IL-6 ELISA KitHuman EOTAXIN-3 ELISA KitHuman IL-10 ELISA KitHuman IL-20 Elisa KitHuman CXCL16 ELISA KitMurine sRANKL ELISA KitHuman IL-17E ELISA KitHuman Resistin ELISA KitHuman EG-VEGF ELISA KitHuman TNF-alpha ELISA KitHuman IFN-gamma ELISA KitHuman GM-CSF ELISA KitHuman MCP-1 ELISA KitHuman RANTES ELISA Development KitHuman SCF ELISA KitHuman MIP-1 alpha ELISA Development KitHuman IP-10 ELISA KitHuman TPO ELISA KitMurine IL-3 ELISA KitMurine IL-4 ELISA KitMurine TNF-alpha ELISA KitMurine GM-CSF ELISA KitRat TNF alpha ELISA Development KitMurine SCF ELSIA KitMurine IL-1-alpha ELISA KitHuman IL-17A ELISA KitRat IL-1-beta ELISA Development KitHuman IL-1-beta ELISA KitHuman IL-12 ELISA KitMurine IL-12 ELISA KitMurine IFN-gamma ELISA KitMurine VEGF ELISA Kit

NEW

NEW

NEW

NEW

NEW

NEW


11


Nucleofection combines the advantages of various

transfection techniques:

1. Straight delivery into the nucleus ensures that expression

starts shortly after transfection and nucleofection of even

non-dividing cells is possible.

2. Upon cell division, DNA located in the cytoplasm can

be transferred into the nucleus, therefore increasing

transfection efficiency even further with time.

3. As DNA is also efficiently delivered into the cytoplasm it

guarantees successful siRNA experiments.

From nucleofection to protein expression in just a few hours

As the DNA can directly enter the cell nucleus, expression

of the transfected protein can be detected shortly after

nucleofection. In cell lines, transfection efficiencies of over

50% can be detected after just 2-4 hours. Even in primary

cells, including non-dividing cells, transgene expression

can be seen shortly after nucleofection. In unstimulated,

resting T cells for example, 15% of the cells express the

transfected gene after just one hour reaching up to 70%

after 16 hours.

Transient protein production offers.

Transient protein expression systems have gained

increasing relevance as they enable fast and flexible protein

production. Protein amounts of up to 10 mg can be

produced within a few days in contrast to production periods

up to several months using stably transfected cell clones.

Transient protein production offers:

l Fast production of small scale protein amounts

l Low costs for cell maintenance

In the past gene transfer into serum-free cultivated

mammalian cells suitable for protein production was

hampered by inefficient transfection methods. The

Nucleofector technology represents the ideal tool for gene

transfer even into hard-to-transfect cell lines like suspension

CHO cells. It has now been successfully tested and

validated for its use in transient protein production.

Benefit from transient protein production using

nucleofection:

l suitable for sCHO, sHEK-293, BHK-21, NS0 and many

other cellsl Applicable even under serum-free conditionsl High transfection efficienciesl Low DNA amounts

Numerous advantages of the Nucleofector technology

High transfection efficiencies: Transfection efficiencies of

over 50% can be obtained in most cell lines as well as in

primary cells. For some primary cells, such as human

dermal fibroblasts, efficiencies exceed 90%.

Short time until analysis: In most cell types, expression of

the transfected gene can be analyzed after just 2-4 hours,

rather than the standard 24 to 48 hours. This means that

Nucleofection - a revolution in transfection

DNA delivery straightinto the nucleus – idealfor transfection of non-dividing cellsNucleofector technology

achieves an efficient direct

transport of the DNA into the

cell nucleus. Consequently

transfection of cells using the

non-viral Nucleofector

technology is no longer

dependent on cell division i.e.

even non-dividing cells such

as resting blood cells or

neurons can be transfected

non-virally with high

efficiencies. The use of viral

systems for gene transfer into

non-dividing cells is therefore

no longer necessary.

From nucleofection to protein expression in just

a few hours.

As the DNA can directly enter the cell nucleus,

expression of the transfected protein can be detected

shortly after nucleofection. In cell lines, transfection

efficiencies of over 50% can be detected after just 2-4

hours. Even in primary cells, including non-dividing

cells, transgene expression can be seen shortly after

nucleofection. In unstimulated, resting T cells for

example, 15% of the cells express the transfected gene

after just one hour reaching up to 70% after 16 hours.

Mission #1: Transfect mouse T cellsIt took just a little bribery and the mouse unveiled the secret totransfecting its T cells. It’s not a trick, it’s nucleofection!Now available for the first time: non-viral transfection of mouse T cellsl up to 40% efficiencyl evaluated for most common

mouse strains: C57BL/6 andBALB/c

l maintenance of functionality

(e.g. stimulation)

Mission #2: Light up your cellsHappy cells shine bright when they are feeling vectorized withpmaxFP. It’s blindingly easy!NEW! pmaxFP fluorescent protein ex-pression vectorsl generation of fluorescent fusion

proteinsl very bright green, yellow or red

fluorescencel license free use for research

purposesl license free use for For-Profit

entities during the first sixmonths after purchase

l suitability for stable expression

Mission #3: Transfect humanmonocytesCatch of the day: human monocytes freshly prepared fornucleofection.Now available for the first time: non-viraltransfection of human monocytes!l Up to 60% efficiency.l High viability.l One technology for DNA and siRNA.l Complete solution - optimized culture

medium included

Mission #4: Protection againstmycoplasmaAntibiotics impressively demonstrate their determination to keepmycoplasma away from your cells.Use amaxa’s highly efficient antibi-otics to keep your cell culture myco-plasma-free!l New antibiotic formulations

against mycoplasmainfection.

l Protection or cure for primarycells or cell line cultures.

l No cytotoxic effects onprimary cells or cell lines.

l Compatible with nucleofection.

Mission #5: Transfect mammalianepithelial cellsWhatever epithelial cells you might have to take care of, theirwelfare couldn’t be commited to trustier hands than those of ourtwo experts.

New! amaxa’s Basic Epithelial CellNucleofector Kit for the transfection ofprimary mammalian epithelial cellsl transfection efficiencies of up to

80%l fast and easy optimization

l applicable for different mammalian

epithelial cells

Mission #6: Transfection of cancerand blood cell linesWith our easy strikers, cell lines formerly known as hard-to-transfect become a pure pleasure to work with. Make every shotcount!

There’s no better non-viraltransfection system for hard-to-transfect cell lines than amaxa’sNucleofector technologyl up to 98% transfection efficiency

e.g. for U-2 OS.

l Complete protocols including

cell culture details.

l up to 81% viability e.g. for A549.

Mission #7: Transfection ofmammalian neuronsIt’s the right combination of electropower and solution skills that keepsyour neurons excited for days.

NEW! amaxa offers first non-viraland highly efficient transfectionsolution for neurons, neural stemcells, astrocytes and oligodendro-cytesl Transfection efficiencies of up to 70%.

l Maintenance of functionality.

l Basic Neuron Kit for virtually all mammalian neurons.

Mission #8: Transfection of cell linesBrilliant transfections of hard-to-transfect cell lines from theproduction line with high efficiencies - almost visible to the nakedeye.

There´s no better non-viraltransfection system for hard-to-transfect cell lines thanamaxa´s Nucleofector

technology:l More than 300 cell lines successfully transfectedl Up to 98% transfection efficiencyl Complete protocols including cell culture details for more than

30 cell linesl Up to 81% viabilityl Optimization for all cell lines within 2 weeks

Mission #9: Transfect mammalianendothelial cellsNeither stifling heat nor spacious constriction can keep our cell-miner team from completing their transfection mission. They reallydig endothelial cells!

New! amaxa’s BasicEndothelial Cell NucleofectorKit for the transfection ofprimary mammalianendothelial cellsl Suitable for virtually any primary mammalian endothelial cell

type.l Fast and easy optimization.l Successfully tested for human, porcine and sheep endothelial

cells.

l Applicable for different mammalian tissue origins.

Mission #10: Transfection of cell lines

Our team’s markmanship is the reason why with nucleofectionthe DNA always enters the nucleus of the target cells.

amaxa’s Nucleofectortechnology - the best non-viral transfection systemfor difficult to transfectcell linesl Up to 84% transfection

efficiency, e.g. for RBL-1l Optimized protocols including cell culture detailsl Up to 94% viability, e.g. for COS-1l Optimization for all cell lines within 2 weeks

Mission #11: Transfection of humanmacrophagesNEW! The first efficient non-viraltransfection method for humanmacrophagesl Up to 60% transfection

efficiencyl maintenance of functionality

(e.g. activation)l Up to 90% viability after 24

hoursl for transfection of resting macrophages

Mission #12: Transfection of difficult-

to-transfect cell linesTransfecting difficult-to-transfect cell lines can be hugefun. It just takes know-how,practice and subtle fingers.Contact our experts to stay onthe ball.

Nucleofector technology- non-viral transfectionof difficult-to-transfect

cell lines!l Up to 85% transfection efficiency, e.g. for Saos-2l Optimized protocols including cell culture detailsl Up to 90% viability, e.g. for KG-1l Optimization for all cell lines within 2 weeks

Mission #13: Transfection of mousemacrophagessThe taming of the mousemacrophages was a major stepto getting them nucleofected.Our cell-charmers will be happyto tell you how it works!

New! The first efficientnon-viral transfectionmethod for mousemacrophagesl Up to 50% transfection efficiency.l Evaluated for macrophages from C57BL/6 and BALB/c strains.l For transfection of resting bone marrow derived macrophages.l Maintenance of functionality.

AMAXA’s Mission 2005

Nucleofection is superior to lipofection fortransient protein production.

In a comparative study the Nucleofector technology (amaxa)

and the lipofection reagent Lipofectamine™ 2000 (Invitrogen)

have been tested in regard to their suitability for transient

protein production. Nucleofection achieves 10-30 times higher

expression rates in CHO cells compared to cells transfected

with lipofection. A similar ratio has been determined for both

the specific and the volumetric productivities.

Combines cells of interest, expression

vector & the appropriate cell type

specific Nucleofector solution &

transfer to an amaxa certified cuvette.

Insert the cuvette into the Nucleofector and choose the cell type specific

Nucleofector program. Press the start button X after a few seconds

nucleofection is completed. Rinse the cuvette with culture medium and

transfer the cells into the culture flask. For 1x10 cells, ten cuvettes have

to be nucleofected.

Culture cells for several days

Harvest of

protein

Why Amaxas Nucleofector Technology is better than Conventional Electroporation!

experiments can be performed much more quickly, and

often completed within a single day.

Simple method: The Nucleofector technology is based

on electroporation, making it a quick method which can

be performed in less than an hour. It consists of the

Nucleofector device, which delivers the electrical

parameters, and Nucleofector Solutions, in which the cells

are contained while the electrical program is executed.

Consequently, the Nucleofector technology requires

minimal optimization (if any) by the user, making it the ideal

tool for gene transfer into primary cells, even by

inexperienced users.

The Industry standard forlabs and research settingFeatures:

Low in lint & low extrable wipers.Non-abresive wipers wont scratch ondelicate surface when used wet

Absorbent wipers hence easily wipe0-up liquid & dustMultifold despensing format enables one at a timedispensing which controls usage.Linguard Anti-Static Polyshield on each pop-up box helpsto further reduce the lint and static buildup.Suggested Area of use: Cleaning & wiping delicatesurfaces such as beakers, lenses, slides, opticle lenses,opticle instruments,surfaces glass layer etc.

For cleanroom applicationKimtech Pure CL 4 WipersSuitable for ISO Class 4 or higher

cleanroomFeatures:100% polypropylene costructionAcid, base & solvent resistantThermal bonding, no adhesive or binders

Delivered in anti-stat bagCan be autoclaved and ETO sterilized

Kimtech Pure CL5 WipersSuitable for ISO Class 5 or higher cleanroomFeaturesOur lowest extractable offeringMade from continuous filament rayon/polyster spun lace blend for a cloth like feel

Contain no binders or surfactantsHigh water absorbancy rate, plus excellent absorptionefficiency and capacityCan be autoclaved and ETO sterilizedSuggested areas to be used: Wiping Bio- reactors,wiping instruments, wiping inlet & outlet pipes

For Clean ManufacturingFeatures:

Made from Polypropylene constructionPerfect for use with oil and other solventsResistant to most of the chemicals andsolvents.Available in different dispensing formatslike: Roll, Flat sheet,Multi-fold, Quarter-fold

Suggested Area of use: Cleaning & wiping media andbio vessels, internal mechinary parts, tool & equipment,at packing points to provide finishing, mopping upspillages on floor.

For Versatile wipingFeatures:Designed to perform the wipingfunction which helps improveproductivity and performanceMade from advancedHYDROKNIT material which

deliver products that are absorbant, higher in strenth andhave more bulk.Bio degradable hence reduce disposable cost andenvironment friendlySuggested Area of Use: WYPALL wipers are an idealproduct to replace your manufactured rags (cloth towelsor rags).Available in different dispensing format like: Roll, Flatsheet, Multi fold, Quarter fold.

Kimberly- Clark ProfessionalPHARMACEUTICAL/BIOTECH

WORKPLACE PRODUCTS

SOLUTION TO ASSIST YOU TO

MAINTAIN BIOCLEANLINESS


12


PES SFCA CN NYL

PES (polyethersulfone) Is fast

and clean. PES is low protein

binding, so there is less change

of removing critical protein from your media. It is

hydrophilic, so no external wetting agents or

surfactants are needed, resulting in low

extractables. And it is fast, so you spend less time

waiting.

SFCA (surfactant-free cellulose

acetate) — It use wetting agents,

typically polyglycol ethers, to

overcome their natural hydrophobicity. These

substances have been shown to be toxic to

specific cell lines in vitro. The NALGENE

surfactant-free cellulose acetate (SFCA)

membrane is the ideal choice if you are growing

sensitive cells. SFCA is also low protein binding.

CN (cellulose nitrate) — CN filter

membranes have been around

since the 1940s. NALGENE CN

membranes are TRITON X® -free and have

excellent wetting properties. CN provides fast flow

rates for buffers and other aqueous solutions. CN

membranes are ideal for filtering and clarifying

solutions when protein binding is not a concern.

NYL (nylon) — NYL has very low

levels of extractables but binds

protein. It’s inherently hydrophilic

so it requires no wetting agents. And Nylon as

better alcohol resistance than any other MF75

Series membrane. Its toughness makes Nylon the

best membrane for special applications.

NALGENE MF75TM PESThe fastest tissue culture

membrane

NALGENE MF75TM SFCAThe cleaner CA membrane

for tissue culture application

NALGENE MF75TM CNThe proven membrane forgeneral-purpose filtration

NALGENE MF75TM NYLThe best membrane

for specializedapplications

NALENGE MF75TM filters are available in different sizes

Ask for it!

Why NALGENE Vacuum Disposable Filter Unitsare better than Competitor

Disposable

Unbeatable speed, low binding and easy to use!

Fast, Low binding and easy to use.

Supor is a registered trademark of Pall Corporation

Supor machV is an extremely clean, fast-flowing

asymmetric PES membrane that provides outstanding

throughput and low protein binding of cell culture media

and other solutions.

These great new filters feature:

l The fast flowing filter to save you time in the lab.

l Easy-to-use ergonomic shape

l Lot identification right on the product

l Guaranteed leak proof closure system

l NALGENE Certified performance

Available in 150mL, 250mL, 500mL, and 1L sizes.

Make Your Lab MORE Productive by Using...NALGENE® MF75™ Filters with New Pall Supor® machV PES Membrane

Filter most difficult to filter fluid such as SERUM very EASILY

Each Filter Unit has a side-arm with a cellulosic vent plug, a quick-

disconnect tubing adapter, and a leakproof threaded closure for

the receiver. Graduations are provided on both the upper and

lower receiver unit, allowing for easy sample assessment. The

Bottle Top Filters are designed to securely screw onto glass media

bottles. All MF75 Series Filters are gamma sterilized and

individually wrapped in easy-to-open bags.

NALGENE Filtration. So much variety, you are sure to be satisfied.Four different membranes and a wide variety of filter sizes, NALGENE offers the tastiest selection of filter units for any lab application.

Choose the best membrane for your application

Product Volume Nalgen M P S

Disposable 115ml 219.00 NA NA NA

150 ml 290.00 865.00 504.00 942.00

250 ml 402.00 900.00 504.00 -

500 ml 761.00 1500.00 790.00 1875.00

1000 ml 928.00 1591.00 790.00 1875.00

Bottle Top 150 ml 278.00 818.00 NA 550.00

250 ml NA 980.00 NA NA

500 ml 390.00 1360.00 NA 608.00

1000 ml 637.00 NA NA 1192.00

Available membrane-

SFCA PVDF PES CA

PES PES Nylon

CN

Nylon

…Because of its Maximum Membrane & Volume Range with LOWEST PRICES

N ALG EN E C orn ing M illiporeSupor m ach V PES m em brane h

PES m em brane h O ld G en M ill ipore h

C N m em brane h h h (m ixed ester)C A m em brane h

SFC A m em brane h

N YL m em brane h h

U nit S tability Excellent Poor PoorC ertif ication of Q uality in each case h h

C ertif ied for pyrogenic ity h h

C ertif ied for sterility h h

C ertif ied for retention h h

C ertif ied for bubble point h h

C ertif ied for f low h h

C ertif ied for m ateria ls h h

Post U se Integrity Testing av ailable h

Shelf li fe (Sterili ty) 5 years 2 years 3 yearsG uaranteed Leakproof C losure h

50m m m em brane diam eter h h

75m m m em brane diam eter h h (70m m ) h (70m m )90m m m em brane diam eter h 1000m l only N oC olour coded collar h h

Pore size identif ied h (on upper) h (on upper) h (on collar)M em brane identif ied h (on upper) h (on upper) h (on collar)C atalog num ber identif ied h (on upper) h (on bag) h (on bag)Lot num ber identif ied h (on upper) h (on bag) h (on bag)C onstruction W elded Friction f it W eldedP ictogram instructions on each bag h

Vacuum tubing adapter A ttached D etached A ttached

New

NALGENE

Metabolic Cage For MiceSpecifically designed for urine and faeces collection from a single mouse

Allow efficient seperation and collection of urine and faeces

New design reduces evaporation and sample loss

l 75% more urine collection and current

metabolic cages means you get adequate

sample volume from even one mouse

l smaller chamber volume and collection tube

design minimizes evaporation

l Proven funnel & cone design with steeper angle

seperates urine & faces into seperate collection

tubes

l Non wetting funnel & cone material ensures

immediate complete seperation of urine &

faces.

l Self-standing for single cage use (Single cage

stand not required)

l Available separately, With support plate will fit

in new Nalgene Metabolic Cage Racks.

NEW

NEW


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