REVERSE PHASE HPLC COLUMNS - Genetix Biotech …genetixbiotech.com/pdf/G-Chrom.pdfSample: 1) Urasil...

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CAT# Product Description Price( )CAT# Product Description Price( )

3252016-17

TM OPTISIL UNIVERSAL COLUMNSTMULTRASIL HYBRID COLUMNS

REVERSE PHASE HPLC COLUMNS

WWW.GENETIXBIOTECH.COM

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TMOptisil HPLC Columns

TMOptisil Column TMOptisil HPLC columns for chemists in the fields of pharmaceuticals, food, drug control, environmental,

agrochemicals cosmetic and academic labs. TMOptisil columns provide the best performance in peak shape, efficiency and resolution. We offer a full line of HPLC

columns to meet HPLC separation and purification needs from analytical to preparative scale. Through continuous

innovation, G-Chrom offers customers the best tools and solutions for separation, purification, and analysis of

organic and bio-organic compounds.

SEM Pictures of Optisil Particles

TMThe Physical Characteristics of Optisil Phases

Phase C18 C8 Phenyl CN NH2 SiO2 CN Diol

Particle size 3, 5, 10 µm 3, 5, 10 µm 3, 5, 10 µm 3, 5, 10 µm 3, 5, 10 µm 3, 5, 10 µm 3, 5, 10 µm 3, 5, 10 µm

Pore Size 120 Å 120 Å 120 Å 120 Å 120 Å 120 Å 120 Å 120 Å

Surface Area 320m2/g 320m2/g 320m2/g 320m2/g 320m2/g 320m2/g 320m2/g 320m2/g

Carbon Loading 17% 12% 13% 7% 6% 0 7% 8%

End-capping Yes Yes Yes Yes No No No No

pH Stability 1.5-10.0 1.5-10.0 1.5-10.0 1.5-9.0 / / 1.5-9.0 /

TMOptisil HPLC Columns Properties:n Ultra high purity (purity > 99.999% SiO2) porous silica particles

n High performance over wide pH range from pH 1.5 to 10.0

n Proprietary surface modification for smooth, uniform and inert silica surface

n Long column lifetime and more injections

n Unique bonding chemistry and end-capping technology

n Exceptional lot-to-lot reproducibility

n Excellent peak shape, especially for polar, acidic and basic compounds

n Available in analytical and prep types, of all dimensions and sample sizes

n Series of stationary reversed phase and normal phases for wide selectivity

n Best value – high premium products with very competitive price

TMOptisil Packing MaterialsOur Optisil HPLC columns are made from spherical, totally porous, and ultra

high purity (>99.999%) type B silica particles. Our proprietary surface

modification before bonding generates a very smooth and uniform surface

with less acidic surface silanols.

Ultra pure and totally porous type B silican Ultra pure, spherical, and totally porous silica

n Narrow pore size and particles size distribution

n Proprietary surface modification to ensure uniform and inert surface

n High surface area for strong retention of hydrophobic and polar compounds

n Enhanced mechanical stability

n Range of particle size from 3 µm to 10 µm for analytical to preparative applications



3272016-17

Optisil C

18 & C

8 Colum

ns

Comparison of Retention of C18 and C8 on Neutral Compounds

min0 5 10 15 20 25 30 35

mAU

0

25

50

75

100

125

150

175

min0 5 10 15 20 25 30 35

mAU

0

50

100

150

200

250

1

2

1

3

3

4

45

6

5

6

TMOptisil C18

TMOptisil C8

Comparison of Retention of C18 and C8

Column: 4.6 x 150 mm, 5 μm

Mobile: 30% water/70% ACN

Temperature: 25 ºC

Flow: 1 ml/min

Detector: 215 nm

Sample: 1) Urasil 2) Ethylbenzene

3) Propylbenzene 4) Butylbenzene

5) Amylbezene 6) Heptylbenzene

min0 2.5 5 7.5 10 12.5 15 17.5

min0 2.5 5 7.5 10 12.5 15 17.5

1

2

3

4

5

67

4

1

2

3

5

67

TMOptisil C18

TMOptisil C8

TMColumn: Optisil XB-C18 and XB-C8, 4.6 x 150 mm, 5μm

Mobile: 78% 20 mM phosphate, pH 2.7 22% MeOH

Flow: 1 ml/min

Detector: 215 nm

Temp: 40 ºC

Samples: 1) Thiourea 2) Benzylamine

3) 3-Aminoquinoline 4) Phthalazine

5) Caffeine 6) 4-Ethylaniline

7) Benzyl alcohol

Separation of Organic Acids

min0 5 10 15 20 25 30 35

1

2

3

4

5

6

7

Column: Optisil C18, 4.6 x 150 mm, 5 µm

Mobile: 25% MeOH/75% 20mM phosphate, pH 2.7

Flow rate: 1 ml/min

Detector: 230 nm

Temp: 40 ¥

Sample: 1) Thiourea 2) 2-Nitrobenzoic acid

3) 4-Hydroxybenzoic acid 4) Phthalic acid

5) 3-Cyanobenzoic acid 6) 2-Acetoxybenzoic acid

7) Benzoic acid

TMAnalysis of Acids: Optisil C18 phase also provides excellent performance for organic acids.

Separation of tricyclic antidepressants (TCAs) at pH 7.0

min0 2 4 6 8 10 12 14 16 18

1 2

3

4 5

Column: Optisil C 1 8 , 4.6 x 150 mm, 5µm

Mobile: 20% 20 mM phosphate, pH 7.0/80%MeOH

Flow rate: 1 ml/min

Detector: 215 nm

Temp: 25 ¥

Sample: 1) Uracil 2) Propranolol 3) Nortriptyline 4) Amitriptyline 5) Trimipramine

Analysis of Strong Bases at Middle pH: The analysis of strong basic compounds

at mid pH provides a further evaluation of the quality of C18 phase because

strong basic compounds such as tricyclic antidepressants (TCAs) are notorious

for poor peak shapes and irreversible adsorption on traditional C18 packing.

The separation of TCAs at pH 7.0 is one of the most important performance

comparisons to clearly distinguish the difference of quality of the columns from

manufacturers. OptisilTM C18 column provides the best peak shape and

resolution for these antidepressants. The high surface coverage and minimal ion

and uniform ion exchange sites of the surface contribute to the excellent peak

shape and chromatographic performance.

TMG-Chrom Optisil C8 - Less Retentive than C18n Selectivity choices for method development optimization

n Good peak shape for basic, acidic and neutral compounds

n High performance over a wide pH range

n Particle sizes from 3 to 10 µm

n Long lifetime with extra dense bonding and double end-capping

TMOptisil C18—Most universal C18 columnTMOptisil C18 columns, featuring high surface coverage and exhaustive end-capping, are an excellent choice as a general-purpose C18 column for a wide variety of organic

compounds. The bonding chemistry is optimized specifically for our base material. Our proprietary double end-capping leads to optimal performance with neutral, polar,

acidic, basic, and chelating compounds.



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il U

HP

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Ultr

asil

HP

LC C

olum

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UHPLC column means high column efficiency, symmetry peak shape and good resolution, and TMfaster than the common column. Optisil UHPLC (1.8µm) column based on our strong

TMresearch advantage. There are many different bonding phases in Optisil UHPLC family. Now we

have these bonding phases: C18, AQ-C18. C8, Phenyl, C18, Polar-RP, C18.

Ultra Resolution: better resolution than that of a normal column that is longer, thicker and has TMmore packing quantity than Optisil UHPLC.

Ultra speed: UHPLC offers more information in unit time and higher speed due to its small

particles.

Sensitivity: higher N, narrower peak width (W), higher peak height. The system sensitivity of 1.8µm

UHPLC is 70% and 40% higher than the system sensitivity of 5µm and 3.5µm.

TMOptisil UHPLC Series: High Efficiency 1.8µ column

TMUltrasil HPLC Columns form the new premium HPLC column series. Compared to mid-range TMpriced Optisil series of premium quality, hybrid silica-polymer particles based UltrasilTM

HPLC columns are our next generation products with superior performance, especially

excellent peak shape for strong bases, and extended lifetime at high pH range, making those

columns most universal HPLC columns for a wide pH range applications. Hybrid silica-TM TMpolymer particles based Ultrasil particles are based on our best seller Optisil series ultra

high purity silica particles, but the surface is further modified with a unique hybrid TMorganic/inorganic polymer using our state-of-art Smooth Coat Surface modification

technique; as a result, the hybrid silica-polymer particles based UltrasilTM particles exhibit

both silica particle properties of high mechanical strength and high efficiency and organic

polymer properties of inertness and resistance to alkali dissolution. Hybrid silica-polymer

particles based UltrasilTM HPLC columns represent our world leading technology of high

premium and high quality HPLC columns in the market. Besides hybrid surface on silica

materials, we also developed PS/DVB polymer based materials for saccharide separation in

UltrasilTM columns, including Saccharide-H and Saccharide-Ca.

Smooth surface of the silica particles

Unique hybrid organic/inorganic polymer

the siloxane bond (Si-O-Si)

O H

S i

O H

S i

O H

S i

O H

O H

O H

O H

O

O

O

O O

O O O O O

O O O

O O O O

O O

O O O O

O O

S i

S i

O H

S i

S i

S i

S i S i

S i S i

S i S i

S i S i

S i

S i

S i

S i S i

O H

S i

TMUltrasil HPLC: The Hybrid Column



3292016-17

Ultrasil H

PLC

Colum

ns

TMHybrid Particles Based UltrasilTMCharacteristics of hybrid particles based Ultrasil columns:

n Wide pH range from 1.0 to 12.5

n Silica particle properties of high mechanical strength and

high efficiency and organic polymer properties of

inertness and resistance to alkali dissolution

n Long column lifetime 5 times of similar product such as

Gemini

n Excellent peak shape for strong bases

Pore Width (Å) 40 60 80 100 200 400 600

0 0

2

4

6

8

BJH Adsorpon dV/dlog(w) Pore Volume

Silica pore size before surface modificaon

Pore V

olu

me (cm

3/g)

�i�ica �o�� i�� a�t�� ac� �o�i� cation

Lifetime Test at Extreme ConditionTMAfter lifetime test at pH 11.5 for a month, the hybrid particle based Ultrasil column shows no apparent efficiency drop.

Unprecedented Peak Shape

At mid pH, the strong bases usually exhibit bad tailing due to the secondary interaction between the analytes and the surface silanols. Because of our Smooth

Coating technique, the hybrid layer totally covers the surface silanols and blocks the access of analytes to surface silanols. The improved bonding and end-capping

further reduce silanol activity. As a result, hybrid particles based UltrasilTM columns show unprecedented peak shapes.

amitriptylineN=17280T=1.28

f

0.00 1.90 3.80 5.70 7.60 9.50 11.40 13.30 15.20

[Unit: min]

amitriptylineN=9410T=1.93

f

0.000 0.900 1.800 2.700 3.600 4.500 5.400 6.300 7.200 8.100

TMThe detection of amitriptyline by Ultrasil C18 The detection of Amitriptyline by Competitor



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Info

rmat

ion

Dimensions Particle Size C18 (Cat. #) C8 (Cat. #) Price (`)

2.1 mm x 100 mm 3µm 21011-10100 21011-20100 30,000

4.6 mm x 150mm 3µm 14011-10100 14011-20100 30,500

4.6 mm x 250mm 3µm 34011-10100 34011-20100 30,500

2.1 mm x 100 mm 5µm 21012-10100 21012-20100 30,000

4.6 mm x 150mm 5µm 14012-10100 14012-20100 30,500

4.6 mm x 250mm 5µm 34012-10100 34012-20100 30,500

Ultrasil HPLC Ordering Information

Optisil UHPLC Ordering Information

Optisil HPLC Ordering Information

Dimensions Particle Size Optimate C18 (Cat. #) Optimate C8 (Cat. #) Price (`)

2.1 mm x 100 mm 3µm 21012-10200 21012-20200 23,200

4.6 mm x 150mm 3µm 14012-10200 14012-20200 23,200

4.6 mm x 250mm 3µm 34012-10200 34012-20200 23,200

2.1 mm x 100 mm 5µm 21013-10200 21013-20200 23,000

4.6 mm x 150mm 5µm 14013-10200 14013-20200 23,000

4.6 mm x 250mm 5µm 34013-10200 34013-20200 23,000

Dimensions Particle Size C18 (Cat. #) Price (`)

2.1 mm x 100 mm 1.8µm 21011-10200 30,500

2.1 mm x 150mm 1.8µm 41011-10200 30,500

4.6 mm x 100mm 1.8µm 93011-10200 30,500

4.6mm × 150mm 1.8µm 14011-10200 30,500

REVERSE PHASE HPLC COLUMNS - Genetix Biotech …genetixbiotech.com/pdf/G-Chrom.pdfSample: 1) Urasil...

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