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IS 7826 (1984): Specification for Ginger Oleoresin [FAD 9:Spices and Condiments]

IS 7826 : 1984

Indian Standard

SPECIFICATION FOR

GINGER OLEORESIN

( First Revision )

First Reprim SEPTEMBER I996

UDC 664.55 : 633.825

BUREAU OF INDIAN STANDARDS

MANAK BHAVAN. 9 BAHADUR SHAH ZAFAR MARG

NEW DEI.HI 110002

Gr3 September I984

(Reaffirmed - 2012)

IS t 7826 - 1984

Indian Standard SPECIFICATION FOR GINGER OLEORESIN

( First Revision )

Spices and Condiments Sectional Committee, AFDC 21

C3airman Rq!vesenting

SHRI HARBANS SINW Ministry of Agriculture ( Department of Agriculture and Cooperation ), New Delhi

M#t?lbWS

AQRIOULTURAL MARKETING Directorate of Marketing and Inspection ( Ministry ADVNER TO GOVERNXENT OF of Rural Development ), Farldabad INDIA

DIREOTOR OF LABORATORIES ( Altarnalc ) DR K. V. AEMED BAVAPPA Central Plantation Crop Research Institute ( ICAR ),

Kaseraged ,_

SHRI DAYA NAND All India Food Preservers Association, New Delhi BRIG DEVINDR~ SrNWi Technical Standardization Committee ( Food

Stuffs ) ( Ministry of Agriculture ), New Delhi SIIRI I3. K. NANDI ( Alfemale )

SUI 0~ P. DIXA~UJA Export Inspection Council of India ( Ministry of

DIREOTOBOB RE~EAROH Conlmerce ), Calcutta.

The I$;otrPlanter’s Association of Southern India, ,

SERI V. B. GANATRA The Bombay Kariana, Colour and Chemical Merchants Association, Bombay

SHRI AJIT K. MAROHANT ( Altarnafr ) Smu C. K. GEORGE Directorate of Cocoa,. Arecanut and Spicea

Development ( Mmistry of Agriculture ), Calicut

SHRI M. S. LAYBH~Y~ANAOEIAR ( Altcrnata ) SHI~I K. V. GEORGE Cardamom Board, Ernakulam SHRI S. K. JAIN

SHRI R. L. MITRA ( Ahrmatr ) ,Botanical survey of India, Calcutta

DR C. K AMPANNA Indian Council of AgriculturaI Research, New Delhi SHRI CHARANDAS V. MARIWALA The Pepper & Ginger Merchant Association,

Bombiy SRRI JAYANTILAL M. GANDHI ( Altrrnatc )

SJUCI J. V. MARIWALA Spices Export Promotion Council,Ernakulam Snnr J. M. GANDHI ( Ahamafc )

( Confinurd on pap 2 )

@ Copyright 1984 BUREAU 0.F INDIAN STANDARDS

This publication is protected under tire Indian Copyri$U Act ( XIV of 1957 ) and reproduction in whole or in part by any means except with written publisher shall be deemed to be an infringement of copyright t!

ermission of the un er the said Act.

. .

. .

r$ I f826 - 1984

( Continuedfrom @ga 1 )

Mmbtrs

SHRI K. U. MATEAI

Da A. G. MATHEW

Representing

Directorate of Health Services, Government of Kerala, Trivandrum

Regional Research Laboratory ( CSIR ), Trivandrum

SI~KI C. S. NARAYANAN ( Al~ermfr ) Smu AJIT K. MEROHAHT Laljee Godhoo & Company, Bombay DR A. G. NAIK-KVRADI~ Dehydrated Products Manufacturer’s Association,

New Delhi DR V. PAUL Hindustan Lever Limited, Bombay SRRI M. RAJOPADHYA State Trading Corporation of India Ltd, New Delhi - _

SHRI K. C. ANANTE I Allarnalr I ) SARI K. GANGADHARAN ( Ahemafe II )

SHRI S. RAMA~WAYY Directorate General of Technical Development ( Ministy of Industries ), New Delhi

SHNI S. N. PANDIEY ( Alteraalr ) SEOR~CTARY Central Committee for Food Standards ( Ministry of

Health and Family Welfare ), New Delhi AE~I~TANT SECRETARY ( Alternate )

SHRI KISIXOHE KUWA~ SHAMJI Indian Pepper and Spice Trade Association, Cochin SHRX MINIKANT V. KXIONA ( Altematr )

SHRI S. B. SINoH Department of Health, Government of Uttar Pradesh, Lucknow

BRIG R. N. VRRYA Quartermaster General’s Branch, Army

LT-COL P. J. CEERIAN ( Allurt~atc ) Headquarters, New Delhi

SHBI P. R. VIRHWAYXBHAR 4~ Central Warehousing Corporation, New Delhi SHRI K. G. MBNON ( Alternate )

SRRI T. PVRNANANDAY, Director General, ISI ( Ew-o&io Mmber ) Director ( Agri & Food ) ( Scnefory,)

Oleoresins Subcommittee, AFDC 21 : 4

DR A. G. MATHEW Regional Research Trivandrum

Laboratory ( CSlR ),

Mnnbns

AGRICULTURAL MARKETINQ Directorate of Marketing & Inspection, Faridabad ~;DV;~ER TO GOV~~~WYENT or

SHRI V. CHA~RYOVLY ( AIfmatr ) SIJRI D. ANIL KVMAB Biological 6 Qrganic Chemicals, Hyderabad DR Cl. K. GEOR~IQ Direhttctz of Cocoa Arecanut Spices Development,

SHRI C. V. JACOB Synthite Industrial Chemicals‘Private Ltd, Cochin SERI R. JAOADIDI~E~ Chillies Export House Limited, Virudhunagar SHRI P. B. KVRUP Techno Chemical Industries Ltd; Calicut DR K. LARIRI

DR S. R. MOORTY ( Altwnatr ) Biological Evans Limited, Hyderabad

SRRI A. K. MARATEIC Saiba Industries Private Limited, Bombay SRRI K. V. MARJWALA The Bombay Oil Industries Private Limited,

DR V. S. SAL~I ( Ahmalr ) Bombay

( Conlinfred ox pusc 10 )

2

IS : 7626 - 1964

SPECIFICATION FOR GINGER OLEORESIN

,( First Revision )

0. FOREWORD

0.1 This Indian Standard ( First Revision ) was adopted by the Indian Standards Institution on 30 April 1984, after the draft finalized by the Spices and Condiments Sectional Committee had been approved by the AgricuItura1 and Food Products Division Council.

0.2 Spices in the form of oIeoresins are now increasingly used by food, pharmaceutical and flavour industries. The oleoresins are preferred as their quality, flavour and strength can be standardized, for better uni- formity of the end product.

0.3 Ginger oleoresin, a reddish brown to dark brown product, is obtained by extraction of dry ginger with pure solvent and subsequent careful removal of the solvent by distillation. It is a mixture of essential oil ( oil of ginger ) and the non-volatile ether extract consisting of pungent principle and some resinous materials.

0.4 Originally this Indian Standard was published in 1975. As a result of knowledge and experience gained with manufacture of ginger oleoresin and technical data gathered, this standard has been revised to include additional solvent of ethyl acetate and certain changes in Table 1 of this standard.

0.5 This standard is subject to the restrictions imposed under the Prevention of Food Adulteration Act, 1954, and the Rules framed thereunder, wherever applicable.

0.6 For the purpose of deciding whether a particular requirement of this standard is complied with, the final value, observed or calculated, expressing the result of a test or analysis, shall be rounded off in accord- ance with IS : 2-1960*. The number of significant places retained in the rounded off value should be the same as that of the specified value in this standard.

*Ruler for rounding off numerical values ( rev&i).

3

ISr7826- 1984

1. SCOPE

1.1 Tlljs standard prescribes the requirements and the methods of sampl- ing and test for ginger oleoresin.

2. TERMINOLOGY

2.1 For the purpose of this standard, the definitions given under 2 of IS : 326-1968’ shall apply,

3. REQUPREMENTS

3.1 Raw Material - Ginger oleoresin shall be obtained by extraction of the ground dried { unless specified otherwise ) rhizomes of <ingi&er ojkinak Roscos ( family zin~iberaceae ) .

3.2 Solvents - The solvents used, either singly or in mixture, shall be as follows*

a) Isopropanol IS : 2631-1976t b) Trichloroethylene IS : 245-1970: c) Acetone IS : 170.19768 ~

d) Ethylene dichloride IS : 869-19761) e) Rectified spirit IS : 323-19597 f) Ethyl acetate IS : 229.1972**

3.3 Description - The material shall be viscous dark brown or reddish brown liquid, with the characteristic odour and flavour of dry ginger and free from extraneous matter and adulterants.

3.4 Odour and Taste - Odour shall be spicy, sweet and aromatic, Taste shall be warm, pungent, biting and cool. The method for assess. ment of odour and taste shall be in accordance with Appendix A,

3.3 The material shall also comply with the requirements given in Table ,I.

*M&odrc of sampling and test for natural and synthetic perfumery material, ( firzi IWlJion ).

tSpecification for isopropyl alcohol. fSpecification for trichloroethylene, technical ( second rrtision ). SSpecification far acetone ( srcond rWiJiOn ).

1ISpeciGcation for ethylene dichloride ( second rsoision ). qSpecifcation for rectified spirit ( rtviscd). **Specification for ethyl acetate.

4

IS : 78iS - 1984

TABLE 1 REQUIREMENTS FOR GINGER OLEORESIN

( Cfuuscs 0.4, 3.5 and 6.1 )

SL CHARAOTERISTIO REQUI~EMEBT APPENDIX No.

METHOD OB TEST, REF TO

#-------,

(1) (21

i) Essential oil

a) Content, percent ( u/m )

b) Specific gravity at 3o”C/3O”C

c) Optical rotation at 30°C

d) ,“,eFOa$ve index ” 1

ii) Ginger01 content. per- cent by mass, Mitt

iii) Residual solvent, mg/ mg, Max

a) Acetone, ethylene dichloride or tri- chloroethylene

b) Isopropanol, or ethyl acetate

c) Hexane

(3) (4)

16 to 35 i -

0.8640 to 0.8758 -

30” to 60’

1,488 0 to 1.497 O,* ( set Note )

15 B

’ Cl No. of 1s1:95;:2-

(5)

-

-

-

Cl No. of’ IS : 326-

1968t

(6)

16.7

5

6

7 .

_-

30 -I I(

50 1

- C

25 j

NOTE - Correction factor for refractive index is WOO0 4% = per degree centigrad.

*Specification for black pepper oleoresin (first revision ). tMethods of sampling and test for natural and synthetic perfumery materials (J*sr

revision ) . L

4. PACKING AND MARKING

4.1 Packing - The material shall be supplied in tightly closed glass, pure aluminium or suitably lined contaicers or containers of high density polyethylene food grade, which shall be nearly full.

4.1.1 The material shall be protected from light and stored in a cool place.

5

IS I 7826 - 1984

4.2 Marking - The container shall be marked with the following pa’rticulars:

a) Name of the material;

b) Manufacturer’s name an*d trade-mark, if any;

c) Net mass of the material when packed; and

d) Batch or code number.

5. SAMPLING

5.1 Representative samples of the material, sufficient to give a composite sample for triplicate determinations, shall be drawn from the containers selected from the lot as prescribed under 3 of IS : 326-1968*.

5.2 Tests for all the characteristics shall be conducted on the composite sample.

5.3 Criteria for Conformity - The lot shall be declared to have conformed to this specification if the results of tests on composite sample meet the corresponding requirements.

6. TESTS METHODS

6.1 Tests shall be conducted as prescribed in 3.4 and co1 4, 5 and 6 of Table I.

6.2 Quality of Reagents - Unless specified otherwise, pure chemicals and distilled water ( see IS : 1070-19771_ ) shall be employed in tests.

NOTE - ‘ Pure chemicals ‘ shall mean chemicals that do not contain impurities which affect the results of analysis.

APPENDIX A ( Clause 3.4 )

ASSESSMENT OFT ODOUR AND TASTE

A-O. OUTLINE OF THE METHOD

A-0.1 The odour and taste of the material is evaluated by comparing with a prepared standard oleoresin.

A-l. PROCEDURE

A-l.1 Preparation of Standard Olesresin - Extract ground, dried, unbleached, ginger by Soxhlet extraction with ethanol or preferably the solvent used by the manufacturer. Distill of ethanol or the solvent completely using high vacuum towards the end.

- *Methods of sampling and test for natural and synthetic perfumery materials (iitJt

reuision ) . tSpecification for water for general laboratory use ( scca~drcuision ).

6

IS : 7826 - 1984

A-1.2 Odour - Test the odour quality of the sample by comparing with the standard oleoresin by smelling strip technique ( see IS : 2284- 1963* ). The material shall be deemed to have passed the test if it gives no off odours but oniy the typical odour of dry ginger. ’

A-1.3 ‘Taste - Weigh out in a beaker 4.5 g of the neutral soup base containing 18 parts of sodium chloride, 22 parts of castor sugar ahd 60 parts of corn flour. Mix into a smooth cream with 10 ml of cold water. Add, while stirring well, 100 ml of boiling water. Place the beaker on a hot-plate and bring the soup to boil for 1 minute. Add 20 mg of sample and stir thoroughly. A similar soup mixed with the standard oleoresin shall be prepared for comparison. The material shall be deemed to have passed the test if the soup has no off-taste.

APPENDIX B [ Tuble 1, Item (ii) ]

DETERMINATION OF GINGEROL CONTENT BY TLC ( THIN LAYER CHROMA’i’OGRAPHY ) METHOD

B-9. OUTLINE OF THE METHOD

B-O.1 Gingerols and other pungent principles of ginger can be separated on TLC silica plates and estimated calorimetrically by reaction with Folin-Denis reagent.

B-l. APPARATUS

B-l.1 TLC Complete Set

B-l.2 Spectropbotometer - Preferably having silica gel1 of 10 mm light path, or a calorimeter.

B-2. REAGENTS

B-2.1 Benzene-Methanol, Reagent Grade ( Developing Solvent ) - Ratio of 10 : 1 ( benzene:methanol ).

B-2.2 Venillin, Reagent Grade - Prepared ‘by dissolving 0.1 g of venillin in 1 000 ml of distilled water.

B-2.3 Sodium Carbonate Solution - Saturated.

B-2.4 Alcohol - 95 percent. _

*Method for olfactory assessment of natural and synthetic petfumery matkrial.

7

IS: 7826 - 1984

B-2.5 Folin-Denis Reagent - Reflux 750 ml of distilled water, 100 g of sodium tungstate, 20 g of phosphomolybdic acid and 5 ml of phos- phoric acid for 2 hours. Cod the mixture and dilute to 1 litre with distilled water.

B-2.6 Silica Gel-G with Binder

B-3. ESTIMATION

B-3.1 Preparation of TLC Plates - Mix 30 g of silica gel containing calcium sulphate as binder with 60 ml of distilled water. Pour the siurry into a TLC spreader adjusted to a thickness of 150 p and spread over 5 to 6 glass plates of 20 x 20 cm. Dry the plates in air for 4 to 5 hours, later activate by drying in an oven at 100 to 105°C for 30 minutes and store in a desiccator.

B-3.2 Preparation of Standard Graph - Pipette out 0.1 to 0.6 ml of standard solution of venillin ( corresponding to 10 to 60 pg of venillin ) mix with 1* ml of Folin-Denis reagent and 1 ml of saturated sodium carbonate solution and make up to 5 ml. Determine the optical density. Then draw a standard graph for ginger01 after multiplying the optical densities obtained at different concentrations with factor 3221152, plotting the quantity of venillin against optical density.

B-3.3 Weigh accurately 5 g of the material, dissolve rn alcohol and make up to 100 ml in a volumetric flask. Spot 3 to 5 ~1 of this solution on a TLC plate and develop with the solvent in an all glass chamber which has been thoroughly saturated with the vapours of the solvent. Keep the plate in the chamber for 5 to 10 minutes after the solvent has reached the maximum height. Expose the plate for 10 to 15 minutes to drive off the solvent and lightly spray with Folin-Denis reagent. Two major blue spots develop, .the spot with lower Rf ( 0.54 to 0.71 ) being waxes and colouring matter and that with lower Rf ( 0.36 to 0.49 ) being gingerols. Scoop the area representing gingerols into a lo-ml centrifuge tube with a small stainless steel scooper or spatula encircling the spot as well as 0.1 to 0.2 cm beyond the spot. Add 3 ml of distilled water and shake well. Pipette 1 ml of Folin-Denis reagent into the tube and mix well. After 3 to 4 minutes add 1 ml of saturated sodium carbonate solution. Mix thoroughly for 5 minutes and keep for 1 hour. Prepare a reagent blank using approximately same area from the silica gel plate and mixing with the reagent. Centrifuge the tubes after 1 hour to separate solids. Read the optical density in a spectrophotometer, at 72.5 rnp or a calorimeter using 660 to G90 mp. Determine the amount of cingcrols in the spot from the standard graph ( see B-3.2) derived by using venillin at different concentrations.

8

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