Introduction to Lab

Safety

Dr. G. Ciptadi, .

Genetics and Breding/ Bioscience Lab.

Dr. Marjuki : Nutrition Lab

Dr. Lilik Eka R. Anim Tech. Prod.

General lab rules

Email: ciptadi@ub.ac.id Blog:gatotciptadi.lecturer.ub.ac.id

Is this what your lab looks like?

Lab Safety:

Principles and practices

employed to protect laboratory

personnel and the environment

from exposure or infection

while working with living

organisms, biological

materials, or agentsX

Lab. Facilities and Safety

Back ground

• Lab. Function ?

teaching and research

• Important to:

Rule Lab. (standart)

Induction (new equipment and staff)

Sharing facilities of lab.

Occupational health and Safety (OHS)

SOP (Standart Operational Procedure)

OH and S Procedure, Responsibilities Staf

and Student

• Comply with safe working procedure: follow safe work

procedure established, OH&S direction

• Use of appropriate personal protective equipment and safety system: where personal protective equipment, use other safety

and emergency equipment provided

• Asssist with the preparation of risk asssesment: when requested, assist the supervisor/staff

• Report OH and S problems:reprort worksplace hazard to supervisor

report injury or illness arising from worksplace activities

Weeks Pokok bahasan Sub pokok bahasan Klas F Kelas

H

1. Pendahuluan/I -Kontrak kuliah , ruang lingkup MK.,

peraturan kerja di laboratorium,

Gatot Gatot

2. Kemanan Lab -Keamanan di laboratorium. Gatot Gatot

3. Kalibrasi peralatan -

Kemanana bhn kimia

-Pengenalan, penanganan dan kalibrasi peralatan

lab.dan bahan kimia

Gatot Gatot

4. Penanganan sampel

(pakan, hasil, produk

dan hasil samping

peternakan, cairan

rumen, darah,

-- Pengenalan macam dan fungsi kertas saring,

milipore, dialisis, serta aplikasinya dalam suatu

eksperimen Persiapan, dan pengambilan

sampel. - Penanganan sampel (Chilling,

Freezing, Drying)

Hartutik Marjuk

i

5. produk fermentasi Produksi gas, NH3, VFA, sintesis protein

mikroba

Hartutik Marjuk

i

6. Instrumentasi analisis

kecernaan in vitro, in

sacco dan in vivo

Prinsip kerja peralatan, pengertian dan cara

penentuan kecernaan in vitro, in sacco dan in

vivo, pakan

Hartutik Marjuk

i

7. sda Prinsip kerja peralatan, pengertian dan cara

penentuan kecernaan in vitro, in sacco dan in

vivo.

Hartutik Marjuk

i

8. UTS UTS UTS UTS

NSF North Mississippi GK-8

9 Spektrofotometri Prinsip kerja, macam dan fungsi

spektrofotometri.

KLAS F Klas

H

Imam T Lilik

R

10 Kromatografi Prinsip kerja, macam dan fungsi

kromatografi.

Imam T Lilik R

11 Elektroforesis Prinsip kerja, macam dan fungsi

elektroforesis.

Gatot Gatot

12 Haemocytometer dan pH

meter, darah

Prinsip kerja dan fungsi

haemocytometer

Imam T Lilik

13 sda Prinsip kerja, macam dan fungsi

pH meter

Imam T Lilik

R

14 Energi dan analisis serat Bomb kalorimeter, dan analisis

serat

Hartutik Marjuki

15 Analisis produk hasil

ternak

Teknik analis telur, susu, daging

dan kulit

Imam T Lilik R

16 UAS UAS

JADWAL RENCANA PRAKTIKUM* Weeks Tanggal Materi Tempat Waktu (Koordinator)

III Pengenalan alat Penimbangan ,

digital dan manual (lab dan

lapang)

Lab. NMT

IV Pengenalan pH Meter,

hemositometer

Lab THT

V

VI Pengenalan alat analisi in vitro,

in sacco, in vivo, produksi gas,

NH3., analisis serat., bom

kalorimeter

Lab. NMT

VII Teknik pengambilan sampel

pakan, feces, urine , cairan

rumen, darah

Lab

sumber

sekar

XIII, IX UTS

X Pengenalan dan penjelasan ttg

fungsi spektrometri

Lab THT )

XI Pengenalan Kromatografi Lab THT (

SAFETY ISSUESP E N I L A I A N :

I. Individual :

• UTS 30 %

• UAS 30 %

• Tugas praktek 20 %

I. Kelompok:

• Diskusi/kuis 10 %

• tugas terstruktur 10 %

• There is always risk!

–The risk must be identified

–The risk is evaluated

–The risk must be measured

–Plan to minimize the risk

Lab Safety

Goal:

Provide the highest practical protection

and the lowest practical exposure

Safe laboratory procedures and

training are needed for everyone!

Important of Lab Safety:

TO PREVENT:

– adverse health effects from exposure to chemicals/bio-

materials

– exposure to organisms, diseases, etc. in laboratories

– laboratory equipment hazards - if not maintained properly

Lab Safety Rules

1. Always follow the teacher’s directions and only do lab work when a teacher is present.

2. Conduct yourself in a responsible manner at all times. No Horseplay!

3. Do not touch any equipment, chemicals, or other materials until told to do so.

4. Do not eat food, drink beverages, or chew gum in the lab. Do not use lab glassware as food or beverage containers

5. Report ALL accidents to your assistance lecturer immediately, even if you think it is minor.

General lab rules• For the chemicals you are working with, you should

be familiar with:

– the standard operating procedure for using that chemical in

your lab (the protocol)

– the hazards associated with that chemical

– the Protokol/procedure required for using that chemical

– storage requirements

– waste disposal procedures

– the procedures to be followed in the event of an emergency

General lab rules (cont.)• Care and followed lab rules exist (Safety

Issues)

• Avoid working alone in the lab

• If you must work after hours or on weekends:

– make arrangements with others in the building to

check in with you periodically.

• Let someone know you are working alone, and make

arrangements to call and check in periodically.

– avoid conducting hazardous experiments during

this time.