Effect of ultrasound on meroplanktonic larvae GUIMARÃES. C 1 ; LA CRUZ. L. T 1 ; NEVES. F. M 1 ; ADAMOWSKI, J. C 2 ; ANDRADE. M. A. B 3 ; LOPES. R. M 1

1 Laboratory of Plankton Systems, Oceanography Institute, University of São Paulo, São Paulo, SP, Brazil 2 Ultrasound,Laboratory, Polytechnic School of the University of São Paulo, SP, Brazil 3 Physics Institute, University of São Paulo, SP, Brazil

Biofouling = +

How does ultrasound affect organisms?

-Promissing method -Reportedly effective on biofilm removal and control -Environmental friendly

Piezoeletric transducers: 28.800 kHz 3D Optical system

Cyprid larvae Materials and methods

introduction

University of São Paulo

1 – Experimental setup: 2 – Example of a frame obtained by one camera 3 – Example of a trajectory 4 – The ultrasound transducer

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Recurrences plots showing the difference of displacement patterns: 5 – Without ultrasound 6 – 5 kPa 7 – 10 kPa 8 – 15 kPa

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Email: c.guimaraes@usp.br

Chemical methods

Physical methods

Solutions?

Good alternative

Transducer

Cyprid larvae

High acoustic pressure =

Less efficient exploring behaviour

Exposition to ultrasound

Velocity Step length Steep turning angles

Recurrence plots:displacement dynamics

White bands: non-estacionary state and transitions between different dynamics

White bands = Random displacement

Results