Dr H Schnerr Leatherhead Food International Ltd., Randalls Road, Leatherhead, Surrey, KT22 7RY +44...
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Dr H Schnerr Leatherhead Food International Ltd., Randalls Road, Leatherhead, Surrey, KT22 7RY +44 (0) 1372 822203 [email protected] Biosensor Technology An Innovative Option for the Food Sector
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Transcript of Dr H Schnerr Leatherhead Food International Ltd., Randalls Road, Leatherhead, Surrey, KT22 7RY +44...
Dr H SchnerrLeatherhead Food International Ltd., Randalls Road, Leatherhead, Surrey, KT22 7RY +44 (0) 1372 822203 [email protected]
www.leatherheadfood.com
Biosensor Technology
An Innovative Option for the Food Sector
SPR Suppliers
Company Instrument Website
Biacore Biacore http://www.biacore.com
Affinity Sensors IAsys http://www.affinity-sensors.com
IBIS Technologies IBIS http://www.ibis-spr.nl
Texas Instruments SPREETA http://www.ti.com
Analytical µ-Systems BIOSUPLAR http://www.biosuplar.de
GWC technologies SPRimager http://www.gwctechnologies.com
Reichert Refractometer-SR7000
http://www.reichertai.com
INIX http://www.inex.org.uk
Objectives of the Experiment
Concentration Analysis: How MUCH? Active Concentration
Solution Equilibrium Inhibition
Affinity Analysis: How STRONG?
KD, KA
Relative Ranking
Yes/No Data Ligand Fishing
Kinetic Rate Analysis: How FAST? ka, kd KD = kd/ka, KA = ka/kd
SPR technology
• Label-free• Real-time • Unique, high quality data on molecular
interactions• Simple assay design• Robust and reproducible• Walk-away automation• Minimal sample preparation• Rapid method
Binding Event Real-time Sensorgram
Biomolecular Binding in Real Time
Biomolecular Binding in Real Time
Principle of Detection
1
RU
Time
1
YYYY
YYYY
1
LED CCD
Sensor chip
Flow cell
Polarisedlight
Reflectedlight
Reflected lightintensity
Reflected lightintensity
Angleof reflection
Angleof reflection
Direct measurement Indirect measurement
Inhibition in solution assay (ISA)
Surface competition assay (SCA)Direct Binding Assay (DBA)
Flexibility in Assay Design
Food Quality Food Safety Food Related Studies Life Science/ Drug D iscovery
A ssay Flexib ility
Assays Relevant to IndustryFood Quality
VitaminsBiotinFolic AcidB12
Pantophenic Acid (B5)
AuthenticityNonmilk Protein in Milk PowderGMO Testing
Process-dependent Food ChangePasteurisation of Milk
Food Quality Food Safety Food Related Studies Life Science/ Drug D iscovery
A ssay Flexib ility
Assays Relevant to IndustryFood Safety
MycotoxinsDONAflatoxin
Antibiotics, growth promoters and veterinarydrug residues
StreptomycinChloramphenicol-agonists
Food PathogensSalmonellaE. coli O157:H7
Food Quality Food Safety Food Related Studies Life Science/ Drug D iscovery
A ssay Flexib ility
Assays Relevant to IndustryFood Related Studies
Protein-Polysaccharide-Interactions-casein and -/-carrageenan
Binding Interaction Studies of Food PathogensSalmonella and Collagen/Muscle ProteinsE. coli O157:H7 and Collagen
Interactions with membranes, receptors etc.Looking for Bioactive Appetite Suppressants
Food Quality Food Safety Food Related Studies Life Science/ Drug D iscovery
A ssay Flexib ility
Assays Relevant to IndustryLife Science/Drug Discovery
ProteomicsLigand FishingSPR/MS
Antibody CharacterisationIsotypingAffinityKinethic
Target IDElucidate Disease MechanismsDefine Drugs or Diagnostic Markers
• Sensor Development
- Biotin/Folic Acid
• Pathogen Detection
- Salmonella
• Allergen Testing• Interaction studies
- Polysaccharide - Protein
• Method Evaluation & Validation• Routine Analysis
- Qflex®Kits for Vitamins
LFI and Biosensors
• SPR detects binding events as changes in mass at the chip surface
• Real-time kinetic measurements• Label-free technology• Qualitative rankings• Flexibility in assay design • Minimal sample preparation• One platform for many different assays
Summary
Biosensor Technology
An Innovative Option for the Food Sector
