Concepts of Geneticselearning.kocw.net/KOCW/document/2013/gacheon/... · Concepts of Genetics ....
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Chapter 5 Lecture
Concepts of Genetics Tenth Edition
Chromosome Mapping in Eukaryotes
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Linked Genes
Mendel’s experiments
Linked Genes
Gene location
Genes on separate chromosomes
Genes on the same chromosome
Gamete types
Equal numbers of all possible allele combinations
More parental combinations than recombinant combinations
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Independent Assortment vs. Gene Linkage
Example from Drosophila Red eyes, x Pink eyes Beige body Ebony body RRBB rrbb F1: Red eyes, Beige body
RrBb
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Independent Assortment vs. Gene Linkage
Testcross: cross to individual of known genotype
F1:Red eyes X Pink eyes Beige body Ebony body RrBb rrbb
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Independent Assortment vs. Gene Linkage
F2 phenotype Number of Offspring
Expected for Unlinked Genes
Red eyes Beige body
398 250
Pink eyes Ebony body
382 250
Red eyes Ebony body
108 250
Pink eyes Beige body
112 250
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Independent Assortment vs. Gene Linkage
F1:Red eyes X Pink eyes Beige body Ebony body RrBb rrbb
RrBb Red Beige
Rrbb Red Ebony
rrBb Pink Beige
rrbb Pink Ebony
rb
RB Rb rB rb
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Independent Assortment vs. Gene Linkage
Coupling or Cis Configuration
If genes are linked:
Red eyes, x Pink eyes Beige body Ebony body
R B R B
r b r b
F1: Red eyes, Beige body R B
r b
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F1: Red eyes, Beige body
Independent Assortment vs. Gene Linkage
R B r b
R B
r b r B
X
R b
Four types of gametes are produced Parental Recombinant
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Independent Assortment vs. Gene Linkage
F1:Red eyes X Pink eyes Beige body Ebony body R B r b r b r b
R B
r b R b r B r b
R B r b R b r B
r b r b r b r b
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Independent Assortment vs. Gene Linkage
F2 phenotype Number of Offspring
Chromosome arrangement
Red eyes Beige body
398 RB//rb Parental
Pink eyes Ebony body
382 rb//rb Parental
Red eyes Ebony body
108 Rb//rb Recombinant
Pink eyes Beige body
112 rB//rb Recombinant
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Genetic Map Units
1% recombination = 1 map unit = 1 centimorgan
108 + 112 x 100 = 22% 1000 These genes are located 22 map units
apart on the same chromosome.
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5.1 Genes Linked on the Same Chromosome Segregate Together
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Genes on the same chromosome demonstrate linkage in genetic crosses. In the absence of recombination all genes on a chromosome would show complete linkage, or non-independent assortment. linkage is 100%, recombination rate is 0%.
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5.2 Crossing Over Serves as the Basis of Determining the Distance between Genes in Chromosome Mapping
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Morgan and Crossing Over
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Alfred Sturtevant demonstrated that the frequency of recombination could be used to order genes along a chromosome
Alfred Henry Sturtevant 1891-1970
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Sturtevant noticed that the recombination rate between loci on the X chromosome varied. He produced the first genetic map where the distance between two loci = % recombination is expressed in terms of map units or centimorgans (cM). 1% recombination = 1 m.u. = 1 cM
Yellow white Miniature
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Single Crossovers
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5.3 Determining the Gene Sequence in Mapping Requires the Analysis of Multiple Crossovers
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Ordering three genes (three-point mapping) must take into account that multiple crossovers can occur per chromosome
Double crossover has a lower rate than single crossover in general.
Single crossover between A and B: 10% Single crossover between B and C: 20% Double crossover: 10% x 20%=2% if the two events are independent.
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A three-point mapping cross involving the yellow, white, and echinus genes in Drosophila melanogaster
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Calculate distance between loci. Map distance between two genes = % of crossovers involving the two genes
Between y and w: 1.5%+0.06%=1.56% Between y and ec: 4%+0.06%=4.06%
F2 pheno=gamete genotype Obs. flies % w+ y+ ec+ 4685 94.4% w y ec 4759 w+ y+ ec 207 4.0% w y ec+ 193 w+ y ec 70 1.5% w y+ ec+ 80 w+ y ec+ 3 0.06% w y+ ec 3
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A Mapping Problem in Maize
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5.4 As the Distance Between Two Genes Increases, the Results of Mapping Experiments Become Less Accurate
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When the distance between two genes is large, the % recombinants does not directly reflect map distance. That is, mapping becomes inaccurate because 2, 3, and more exchanges go undetected.
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5.5 Drosophila Genes Have Been Extensively Mapped
Actually, the Drosophila genome has been sequenced
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5.6 Lod Score Analysis and Somatic Cell Hybridization Were Historically Important in Creating Human Chromosome Maps
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5.7 Chromosome Mapping is Now Possible Using DNA Markers and Annotated Computer Databases
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5.8 Crossing Over Involves a Physical Exchange between Chromatids
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Recombination Occurs between Mitotic Chromosomes
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5.9 Exchanges Also Occur between Sister Chromatids